Article
Biochemistry & Molecular Biology
Antonio Galindo, Vicente J. Planelles-Herrero, Gianluca Degliesposti, Sean Munro
Summary: TRAPP complexes, which are nucleotide exchange factors, play vital roles in membrane traffic and autophagy. TRAPPII activates Rab11, while TRAPPIII activates Rab1, with shared core subunits affecting nucleotide exchange and specific large subunits essential for in vivo activity. Cryo-EM structures reveal the assembly of TRAPPIII complex, showing how TRAPPIII-specific subunits hold the catalytic core and large arms determine specificity. The lower resolution structure of TRAPPII suggests evolution from a single ur-TRAPP.
Article
Cell Biology
Azumi Yoshimura, Stephanie Miserey-Lenkei, Evelyne Coudrier, Bruno Goud
Summary: Arp2/3 inhibition affects the acetylated microtubule network and induces abnormal accumulation of long RAB1/GM130-positive carriers, resulting in a decreased rate of anterograde cargo arrival at the Golgi apparatus.
Article
Plant Sciences
Takehiko Kanazawa, Ryuichi Nishihama, Takashi Ueda
Summary: Eukaryotic cells possess endomembrane organelles that require a tightly regulated membrane trafficking system for transportation and localization. This study discovered that a mutation in a COPI subunit affects the morphology of oil bodies in Marchantia polymorpha, suggesting the involvement of cell wall biosynthesis in shaping these organelles.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Yoko Ito, Tomohiro Uemura
Summary: Compared to the static image of plants, the world inside each cell is dynamic. Visualizing the working components inside cells using super-resolution microscopy is crucial for understanding cellular events, particularly in the study of plant membranes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Galina Beznoussenko, Hee-Seok Kweon, Irina S. Sesorova, Alexander A. Mironov
Summary: This study compared the explanatory power of the cisterna maturation-progression model and the kiss-and-run model for the intra-Golgi transport. The authors found that their observations were more in line with the kiss-and-run model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Joanne Chia, Shyi-Chyi Wang, Sheena Wee, David James Gill, Felicia Tay, Srinivasaraghavan Kannan, Chandra S. Verma, Jayantha Gunaratne, Frederic A. Bard
Summary: The Src tyrosine kinase promotes the relocation of GALNTs enzymes by phosphorylating GBF1, inducing tubular transport carriers formation. This mechanism of tyrosine phosphorylation-mediated regulation may be a conserved and widespread mechanism in GEF-Arf complexes.
Review
Cell Biology
Akihiko Nakano
Summary: The structure of the Golgi apparatus varies among different species, but its functions remain conserved. In addition to the core layers of cisternae, the Golgi is accompanied by neighboring compartments on its cis and trans sides. High-resolution live imaging has revealed striking similarities in the behaviors of these compartments, indicating the presence of conserved mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Virology
Maria C. Gimenez, Yesica R. Frontini-Lopez, Cristian A. Pocognoni, Julieta S. Roldan, Clara Garcia Samartino, Marina Uhart, Maria Colombo, Mauricio R. Terebiznik, Laura R. Delgui
Summary: This article investigates the replication mechanism of infectious bursal disease virus (IBDV) in host cells. The study found that IBDV establishes replication complexes on endosomes associated with the Golgi complex by hijacking the endocytic pathway. Additionally, the study discovered that the Rab1b-GBF1-ARF1 axis plays a crucial role in IBDV replication. These findings contribute significantly to the understanding of birnavirus-host cell interactions and the proposed evolutionary connection role of birnaviruses.
JOURNAL OF VIROLOGY
(2022)
Review
Immunology
Tomohiko Taguchi, Kojiro Mukai, Eiko Takaya, Ruri Shindo
Summary: DNA is present in the nucleus and mitochondria of eukaryotic cells, but can also be found in the cytosol in certain instances. The cytosolic DNA consists of self DNA leaked from the nucleus or mitochondria, as well as non-self DNA from DNA viruses, which triggers the host immune response. Recent studies have identified key molecules, cGAS and STING, in this immune response, with STING playing a crucial role in triggering various cellular responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lilian do Amaral, Neife Aparecida Guinaim dos Santos, Flavia Malvestio Sisti, Elaine Del Bel, Antonio Cardozo dos Santos
Summary: This study demonstrates the neurotrophic potential of doxycycline by inducing neurite outgrowth through activation of the trkA receptor and downstream signaling pathways, without inducing the expression of NGF. Additionally, doxycycline increased the expression of proteins involved in axonal and synaptic plasticity, suggesting a potential role in restoring neuronal connectivity lost at the initial phase of neurodegeneration.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Review
Biochemistry & Molecular Biology
Jose angel Martinez-Menarguez, Emma Martinez-Alonso, Mireia Cara-Esteban, Monica Tomas
Summary: Parkinson's disease is the second most common neurodegenerative disease characterized by the loss of dopaminergic neurons and the formation of Lewy bodies in neurons. Rab1 is a key protein involved in maintaining cellular function and signaling pathways, directly linked to the pathogenesis of PD and other neurodegenerative diseases. This GTPase is considered a potential candidate for therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Emi Ito, Tomohiro Uemura
Summary: This article mainly introduces the basic principles of membrane traffic and its diversity in plants. Through the study of regulators in the trans-Golgi network, it summarizes their functions in different transport pathways and their roles in plant development and environmental stimuli.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Aaron M. N. Joiner, Ben P. Phillips, Kumar Yugandhar, Ethan J. Sanford, Marcus B. Smolka, Haiyuan Yu, Elizabeth A. Miller, J. Christopher Fromme
Summary: The study revealed the regulation of Rab1 activation by the TRAPPIII complex, with the Trs85 subunit serving as a membrane anchor for the entire complex via its amphipathic helix. These findings provide a structural understanding of Rab activation on organelle and vesicle membranes.
Article
Biochemistry & Molecular Biology
Ahmed Elkamhawy, Jiyu Woo, Noha A. Gouda, Jushin Kim, Hossam Nada, Eun Joo Roh, Ki Duk Park, Jungsook Cho, Kyeong Lee
Summary: In this study, 24 melatonin analogues were synthesized as novel MAO-B inhibitors, with 12 compounds showing high inhibition rates. Compounds 3n and 3r exhibited good selectivity indices and have potential roles in the treatment of oxidative stress in Parkinson's disease.
Article
Endocrinology & Metabolism
Miroslav P. Milev, Djenann Saint-Dic, Khashayar Zardoui, Thomas Klopstock, Christopher Law, Felix Distelmaier, Michael Sacher
Summary: TANGO2 variants lead to a complex disease phenotype involving muscle breakdown, encephalopathy, seizures, and defects in membrane trafficking and mitochondrial function. The dual role of TANGO2 in membrane trafficking and mitochondria physiology partially explains the phenotype of affected individuals.
JOURNAL OF INHERITED METABOLIC DISEASE
(2021)
Article
Medicine, General & Internal
Jose Manuel Sanchez-Villalobos, Alfredo Serne-Berna, Juan Salinas-Ramos, Pedro Pablo Escolar-Perez, Emma Martinez-Alonso, Daniel G. Achel, Miguel Alcaraz
Summary: The study retrospectively analyzed the local control and survival of breast cancer brain metastases patients treated with VMAT-RS, showing that treatment modality and patient baseline situation significantly influence patient survival.
Article
Clinical Neurology
Angel Aledo-Serrano, Alfredo Serna-Berna, Juan Salinas-Ramos, Emma Martinez-Alonso, Miguel Alcaraz-Banos, Jose Manuel Sanchez-Villalobos, Jose Antonio Perez-Vicente
Summary: The study found that levetiracetam is the most commonly used antiseizure medication in patients with brain metastasis-related epilepsy treated with radiosurgery, but common psychiatric comorbidities in this population may influence the choice of medication.
Article
Nutrition & Dietetics
Alejandro Villasanta-Gonzalez, Juan Francisco Alcala-Diaz, Cristina Vals-Delgado, Antonio Pablo Arenas, Magdalena P. Cardelo, Juan Luis Romero-Cabrera, Fernando Rodriguez-Cantalejo, Javier Delgado-Lista, Maria M. Malagon, Pablo Perez-Martinez, Matthias B. Schulze, Antonio Camargo, Jose Lopez-Miranda
Summary: The study identified a plasma fatty acid profile associated with the development of T2DM, which can be used as a predictive biomarker. Patients with higher FA scores were more likely to develop T2DM and had lower insulin sensitivity and higher hepatic insulin resistance.
EUROPEAN JOURNAL OF NUTRITION
(2022)
Review
Biochemistry & Molecular Biology
Jose angel Martinez-Menarguez, Emma Martinez-Alonso, Mireia Cara-Esteban, Monica Tomas
Summary: Parkinson's disease is the second most common neurodegenerative disease characterized by the loss of dopaminergic neurons and the formation of Lewy bodies in neurons. Rab1 is a key protein involved in maintaining cellular function and signaling pathways, directly linked to the pathogenesis of PD and other neurodegenerative diseases. This GTPase is considered a potential candidate for therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Ivan Arias de la Rosa, Maria Dolores Lopez-Montilla, Cristobal Roman-Rodriguez, Carlos Perez-Sanchez, Ignacio Gomez-Garcia, Clementina Lopez-Medina, Maria Lourdes Ladehesa-Pineda, Maria Del Carmen Abalos-Aguilera, Desiree Ruiz, Alejandra Maria Patino-Trives, Maria Luque-Tevar, Isabel Anon-Onate, Maria Jose Perez-Galan, Rocio Guzman-arbarroja, Maria M. Malagon, Chary Lopez-Pedrera, Alejandro Escudero-Contreras, Eduardo Collantes-Estevez, Nuria Barbarroja
Summary: The study evaluated the clinical and molecular signs of cardiovascular disease (CVD) in Psoriatic Arthritis (PsA) patients and their relationship with disease features. It also identified a patient profile that could benefit from treatment with methotrexate (MTX) and/or apremilast to reduce CVD risk. The results showed that cardiometabolic comorbidities were associated with disease activity and inflammatory status in PsA patients. Certain CVD-related molecules were found to be altered in these patients and associated with key clinical features. Different patient phenotypes were identified based on the rates of cardiometabolic comorbidities, and their response to treatment varied. The study suggests that novel CVD-related proteins could be therapeutic targets in PsA, and apremilast could be an effective treatment for patients with high CVD risk.
JOURNAL OF INTERNAL MEDICINE
(2022)
Article
Medical Laboratory Technology
Maria Del Carmen Navarro-Ruiz, Jaime Lopez-Alcala, Alberto Diaz-Ruiz, Sandra Diaz Del Moral, Carmen Tercero-Alcazar, Andrea Nieto-Calonge, Jose Lopez-Miranda, Francisco J. Tinahones, Maria M. Malagon, Rocio Guzman-Ruiz
Summary: Obesity is a rapidly growing pathological condition worldwide, with altered lipid accumulation in adipose tissue as one of the main causes. This study explores the molecular mechanisms of adipose tissue dysfunction in conditions of obesity and insulin resistance through the study of protein acetylation modifications. The results reveal different acetylation patterns in subcutaneous and omental adipose tissue, and the impact of acetylation on the function of FABP4, highlighting the molecular complexity of adipose tissue.
TRANSLATIONAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Ivan Arias-de la Rosa, Alejandro Escudero-Contreras, Miriam Ruiz-Ponce, Laura Cuesta-Lopez, Cristobal Roman-Rodriguez, Carlos Perez-Sanchez, Patricia Ruiz-Limon, Rocio Guzman-Ruiz, Fernando Leiva-Cepas, Juan Alcaide, Pedro Segui, Chamaida Plasencia, Ana Martinez-Feito, Pilar Font, Maria C. Abalos, Rafaela Ortega, Maria M. Malagon, Francisco J. Tinahones, Eduardo Collantes-Estevez, Chary Lopez-Pedrera, Nuria Barbarroja
Summary: This study investigated the association between adipose tissue dysfunction, autoimmunity, and disease activity in rheumatoid arthritis (RA). It was found that insulin resistance and altered adipocytokine profile were related to disease activity, the presence of anti-citrullinated protein antibodies (ACPAs), and worse response to therapy in RA. Adipose tissue in the context of arthritis exhibited an inflammatory state with macrophage and B/plasma cell infiltration, and ACPAs could induce inflammation and insulin resistance in macrophages while promoting defective adipocyte differentiation. Biologicals partially restored this defect.
Article
Medicine, General & Internal
Marina Mora-Ortiz, Juan F. Alcala-Diaz, Oriol Alberto Rangel-Zuniga, Antonio Pablo Arenas-de Larriva, Fernando Abollo-Jimenez, Diego Luque-Cordoba, Feliciano Priego-Capote, Maria M. Malagon, Javier Delgado-Lista, Jose M. Ordovas, Pablo Perez-Martinez, Antonio Camargo, Jose Lopez-Miranda
Summary: This study identified 12 endogenous metabolites with the potential to predict T2DM remission. These metabolites, combined with clinical variables, can be used to provide more precise therapy.
Article
Multidisciplinary Sciences
Marta Barradas, Adrian Plaza, Gonzalo Colmenarejo, Iolanda Lazaro, Luis Filipe Costa-Machado, Roberto Martin-Hernandez, Victor Mico, Jose Luis Lopez-Aceituno, Jesus Herranz, Cristina Pantoja, Hector Tejero, Alberto Diaz-Ruiz, Fatima Al-Shahrour, Lidia Daimiel, Viviana Loria-Kohen, Ana Ramirez de Molina, Alejo Efeyan, Manuel Serrano, Oscar J. Pozo, Aleix Sala-Vila, Pablo J. Fernandez-Marcos
Summary: The study shows that fasting can provide protection from chemotherapy-associated toxicity. They found that the profile of fatty acids in erythrocyte membranes and gene expression in peripheral blood mononuclear cells are associated with the benefits of fasting during cancer treatment in mice and patients.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Samuel M. Lee, Jose Muratalla, Saman Karimi, Alberto Diaz-Ruiz, Maria Dolores Frutos, Grace Guzman, Bruno Ramos-Molina, Jose Cordoba-Chacon
Summary: Non-alcoholic steatohepatitis (NASH), characterized by hepatic peroxisome proliferator-activated receptor gamma (PPAR gamma) expression and obesity, was investigated in mice. Hepatocyte-specific deletion of PPAR gamma attenuated NASH progression in male and female mice with high fat, cholesterol, and fructose (HFCF) diet. Transcriptomic and metabolomic analysis showed alterations in amino acid metabolism, and PPAR gamma deletion increased betaine-homocysteine S-methyltransferase expression and reduced homocysteine levels. Additionally, in obese patients, NASH was associated with increased insulin resistance and hepatic PPAR gamma expression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
M. Carmen Navarro-Ruiz, M. Carmen Soler-Vazquez, Alberto Diaz-Ruiz, Juan R. Peinado, Andrea Nieto Calonge, Julia Sanchez-Ceinos, Carmen Tercero-Alcazar, Jaime Lopez-Alcala, Oriol A. Rangel-Zuniga, Antonio Membrives, Jose Lopez-Miranda, Maria M. Malagon, Rocio Guzman-Ruiz
Summary: This study investigated the carbonylome of subcutaneous and omental human adipose tissue in obesity-associated insulin resistance. The results showed differences in carbonylated proteins between different fat depots, highlighting the complexity of oxidative stress at the metabolic level. Furthermore, the study revealed the altered expression and function of carbonylated transferrin, linking oxidative stress to metabolic dysfunction. This research provides insights into the molecular mechanisms underlying adipocyte dysfunction in obesity and identifies potential targets for therapeutic intervention.
Article
Anatomy & Morphology
Mireia Cara-Esteban, Maria Pilar Marin, Emma Martinez-Alonso, Sergio Martinez-Bellver, Vicent Teruel-Marti, Jose A. Martinez-Menarguez, Monica Tomas
Summary: Gastrointestinal disorders are early consequences of Parkinson's disease and significantly affect the enteric nervous system. The Golgi complexes of enteric dopaminergic neurons appear fragmented in a rat model of Parkinson's disease, suggesting their potential as early indicators of the disease.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Cell Biology
Monica Tomas, Emma Martinez-Alonso, Narcisa Martinez-Martinez, Mireia Cara-Esteban, Jose A. Martinez-Menarguez
Summary: Fragmentation of the Golgi ribbon is a common feature of Parkinson's disease and other neurodegenerative diseases, possibly caused by alterations in regulatory proteins. While most information is derived from cellular and animal experimental models, further research on human tissue is needed to better understand the mechanisms underlying these diseases.
HISTOLOGY AND HISTOPATHOLOGY
(2021)