Article
Neurosciences
Cristina E. Maria-Rios, Geoffrey G. Murphy, Jonathan D. Morrow
Summary: There are intrinsic differences in excitability between medium spiny neurons (MSNs) in the NAc core and shell, with shell MSNs being more excitable, having greater input resistance, lower cell capacitance, and faster firing frequency. These differences may be linked to the distinct anatomical characteristics and functional roles of core and shell MSNs in reward learning.
Article
Neurosciences
Rajkamalpreet S. Mann, Brian L. Allman, Susanne Schmid
Summary: Disruptions in the CNTNAP2 gene are associated with language impairments and symptoms of autism spectrum disorder (ASD). Knocking out this gene in rodents leads to ASD-like symptoms, including auditory processing deficits. This study found that CNTNAP2 is crucial for maintaining intrinsic neuronal properties and synaptic wiring in the developing auditory cortex.
JOURNAL OF NEUROPHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Bingxue Li, Songling Li, Honglan Zheng, Zhiqiang Yan
Summary: Research shows that in Drosophila larvae, the MET currents in Cho neurons are mediated by two TRPV channels, NAN and IAV, with the C-terminal residues 857 through 990 of IAV regulating the kinetics of MET currents. Additionally, the NompC channel contributes to the adaptation of auditory transduction currents.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Thawann Malfatti, Barbara Ciralli, Markus M. Hilscher, Steven J. Edwards, Klas Kullander, Richardson N. Leao, Katarina E. Leao
Summary: This study aimed to target specific neuronal populations in the dorsal cochlear nucleus (DCN) using genetic tools, but found limitations in the ability to target excitatory neurons with the CaMKII alpha promoter. Stimulation and activation of certain proteins could increase firing rates of excitatory neurons, potentially aiding in tinnitus research.
Article
Neurosciences
Na Jin, Su-Yue Zhu, Xin-Yu Yang, Cheng Zhen, Yan Li, Huan-Huan Zhang, Ai-Ping Xu, Meng-Ya Wang, Chao Zheng
Summary: Orexin-A potentiates glycine currents in spinal cord ventral horn neurons by activating OX1R, likely through an IP3/Ca2+-dependent PKC signaling pathway.
BRAIN RESEARCH BULLETIN
(2021)
Article
Neurosciences
Yan Li, Xin-Yu Yang, Na Jin, Cheng Zhen, Su-Yue Zhu, Wan-Yu Chu, Huan-Huan Zhang, Ai-Ping Xu, Jie Wu, Meng-Ya Wang, Chao Zheng
Summary: This study aimed to investigate the effects of pilocarpine on glycine receptors in spinal cord neurons and the underlying mechanisms. The results showed that pilocarpine increased glycine currents through the activation of M3-AChRs and IP3/Ca2+/PKC pathways.
NEUROSCIENCE LETTERS
(2022)
Article
Neurosciences
A. Ilari, L. Curti, M. Petrella, N. Cannella, A. La Rocca, G. Ranieri, E. Gerace, D. Iezzi, L. Silvestri, G. Mannaioni, R. Ciccocioppo, A. Masi
Summary: Previous studies have shown that moderate ethanol drinking suppresses the spontaneous activity of VTA dopamine neurons while increasing their response to acute ethanol. Chronic ethanol consumption increases GABA input frequency and leads to rewiring of the GABA network in the VTA, potentially driving progression to addiction.
Article
Neurosciences
Lin Lin, Jay Campbell, Donata Oertel, Philip H. Smith
Summary: T-stellate cells in the ventral cochlear nucleus make excitatory connections with each other and inhibitory connections with glycinergic cells, playing a significant role in auditory processing.
JOURNAL OF COMPARATIVE NEUROLOGY
(2022)
Article
Multidisciplinary Sciences
Lutz Gaertner, Thomas Lenarz, Andreas Buechner
Summary: In ABI patients, LEP measurements can predict auditory perception with high sensitivity and have the potential to support fitting. LEP thresholds are highly correlated with behavioral thresholds and most comfortable levels.
Article
Cell Biology
Adrien J. R. Molinas, Lucie D. D. Desmoulins, Roslyn K. K. Davis, Hong Gao, Ryousuke Satou, Andrei V. V. Derbenev, Andrea Zsombok
Summary: Stimulation of hepatic sympathetic nerves increases glucose production and glycogenolysis, and the activity of pre-sympathetic neurons in the PVN and VLM/VMM largely influences sympathetic output. The excitability of liver-related PVN neurons increased in high-fat diet (HFD)-fed mice, and insulin suppressed the firing activity of liver-related PVN and pre-sympathetic VLM/VMM neurons in HFD mice, suggesting that HFD alters the excitability of pre-autonomic neurons as well as their response to insulin.
Article
Biochemistry & Molecular Biology
Milica Gusic, Klaus Benndorf, Christian Sattler
Summary: Our study reveals that each occupied binding site contributes to channel activation in P2X7 receptors. While the occupation of a single binding site can activate the channels, three bound ligands maximally stabilize the open state, suggesting a stepwise activation process for P2X7 receptors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Neurosciences
Christopher M. Lee, Renee N. Sadowski, Susan L. Schantz, Daniel A. Llano
Summary: Early developmental exposure to PCBs leads to long-lasting changes in both inhibitory and excitatory neurotransmission in the auditory cortex, which are independent of peripheral hearing changes.
Article
Instruments & Instrumentation
Ian Delgadillo Bonequi, Abraham Stroschein, Lucas J. Koerner
Summary: This article describes a custom and open-source FPGA-based data acquisition system that is used for electrophysiology and closed-loop feedback experiments. The system combines FPGA acquisition and processing with high-speed analog and digital converters to enable real-time feedback. The digital approach simplifies experimental setup and repeatability by allowing for system identification and in situ tuning of filter bandwidths.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Neurosciences
Dennis J. Weingarten, Eva Sebastian, Jennifer Winkelhoff, Nadine Patschull-Keiner, Alexander U. Fischer, Simon L. Wadle, Eckhard Friauf, Jan J. Hirtz
Summary: This study reveals a glycinergic projection from the ipsilateral cochlear nucleus to principal neurons in the lateral superior olive, providing inhibitory synaptic input to mediate sidebands of neural response.
FRONTIERS IN NEURAL CIRCUITS
(2023)
Article
Biochemistry & Molecular Biology
Yi Zhang, Huan Gui, Zikun Duan, Tian Yu, Jie Zhang, Xiaoli Liang, Chengxi Liu
Summary: Systemic activation of D1 receptors has been found to promote emergence from propofol-induced unconsciousness, with the nucleus accumbens (NAc) showing disinhibited neuronal activity during the recovery phase. D1 receptor modulation on the activity of NAc MSNs plays a vital role in facilitating the emergence from propofol-induced unconsciousness, as shown by whole-cell patch clamp recordings and pharmacological intervention experiments.
NEUROCHEMICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
D. Duzgun Ergun, S. Dursun, N. Pastaci Ozsobaci, M. Naziroglu, D. Ozcelik
Summary: This study elucidated the relationship between hypercapnia and the TRPM2 channel, as well as the effects of zinc, selenium, and glutathione. Experimental results showed that hypercapnia increased oxidative stress, but zinc, selenium, and glutathione were found to protect cells from damage caused by oxidative stress.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yusuf Cagdas Kumbul, Mustafa Naziroglu
Summary: This study investigates the effects of paclitaxel (PAX) and melatonin (MLT) on human laryngeal squamous cancer cells (Hep2) and the underlying mechanisms. The results show that PAX and MLT can induce cell death and apoptosis in Hep2 cells by affecting intracellular Ca2+ and Zn2+ levels. Furthermore, MLT treatment enhances the anticancer effects of PAX and reduces resistance to PAX. The findings also suggest that TRPM2 plays a critical role in this process.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Correction
Biochemistry & Molecular Biology
Zine Kechrid, Malika Hamdiken, Mustafa Naziroglu, Manuel Flores-Arce
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Endocrinology & Metabolism
Mustafa Naziroglu
Summary: Oxidative stress-induced activation of TRPM2 and TRPV4 channels can cause neuronal and cellular injury. This study discovered the potential antagonist properties of carvacrol (CARV) against TRPM2 and TRPV4 activations induced by oxidative stress, suggesting a protective role of CARV in neurodegenerative diseases associated with oxidative stress.
METABOLIC BRAIN DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Ozgur Ocal, Mustafa Naziroglu
Summary: This study investigates the combined treatment of CiSP and EPA in DBTRG cells and finds that the activation of TRPM2 channel can enhance the anticancer, apoptotic, and oxidant actions of CiSP. Therefore, stimulating TRPM2 via EPA could be an effective approach in the treatment of glioblastoma tumors.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Neurosciences
Ozgur Ocal, Aymer Cosar, Mustafa Naziroglu
Summary: This study investigates the protective role of amantadine (AMN) against hypoxia-induced brain injury and apoptosis. The results suggest that AMN attenuates oxidative neurotoxicity, inflammation, and apoptosis by inhibiting the activation of TRPM2 and TRPV4 channels, thus reducing excessive calcium influx and mitochondrial free reactive oxygen species (mitROS) in neurons. These findings provide new insights into the neuroprotective effects of AMN against hypoxia and suggest its potential therapeutic application for neurodegenerative and cerebrovascular diseases associated with mitROS, calcium, and zinc dysregulation.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Ophthalmology
Hatice Daldal, Mustafa Naziroglu
Summary: Rituximab (RTX) may have protective effects on LPS-induced adverse actions in retinal pigment epithelial cells by inhibiting TRPM2 channel.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2022)
Article
Ophthalmology
Hatice Daldal, Mustafa Nazirogluz
Summary: This study investigated the modulatory effect of carvacrol on cell apoptosis and retinal oxidative damage induced by high blood glucose. The results showed that carvacrol alleviated cell death and oxidative stress caused by high blood glucose by inhibiting the activity of the TRPM2 channel.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2022)
Article
Endocrinology & Metabolism
Ahmi Oz, Ramazan Cinar, Mustafa Naziroglu
Summary: This study investigated the cytosolic localization of TRPV1 and its relationship with oxidative neurotoxicity in glial cells. The results showed that TRPV1 was expressed in the perinuclear area of glial cells and not in the cell membrane. In DBTRG cells, capsaicin stimulation activated TRPV1 channels, leading to an increase in intracellular free reactive oxygen species and cell apoptosis. These effects were diminished by the treatment of a TRPV1 blocker.
METABOLIC BRAIN DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Bunyamin Aydin, Mustafa Naziroglu
Summary: This study investigated the effects of selenium and curcumin on STZ-induced neuropathic pain in mice by modulating TRPM7 activity. The results showed that STZ activation of TRPM7 led to neuropathic pain, oxidative neurotoxicity, and apoptosis in the mice. However, treatment with selenium and curcumin reduced these effects by downregulating TRPM7 activity.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yener Akyuva, Mustafa Nazirog
Summary: In this study, we investigated the effects of silver nanoparticles (AgNPs) and cisplatin (CiSP) exposure on glioblastoma cells through the activation of TRPM2 cation channels. We found that the cytotoxic and apoptotic actions of CiSP were enhanced by the treatment of AgNPs, which activated TRPM2. The oxidant actions of AgNPs were mediated by various cellular processes involving mitochondrial membrane depolarization, lipid peroxidation, and ROS generation. The combination of AgNPs and CiSP showed a synergistic action in the treatment of glioblastoma tumor cells through the stimulation of TRPM2.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Muge Mavioglu Kaya, Inan Kaya, Mustafa Naziroglu
Summary: The activation of TRPA1, TRPM2, and TRPV1 channels increases apoptotic and oxidant actions in colon cancer cells. Sambucus ebulus L (SEB) treatment inhibits the actions of these channels, thereby reducing colon tumor growth. Hence, TRPA1, TRPM2, and TRPV1 activations could be potential targets for colon tumor treatment.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Pharmacology & Pharmacy
Kemal Ertilav, Mustafa Naziroglu
Summary: The study found that melittin (MLT) treatment can enhance the cytotoxic effects of cisplatin (CSP) on tumor cells by modulating Ca2+ channels. This enhancement is achieved through the activation of TRPM2 channels, which leads to the accumulation of mitochondrial reactive oxygen species (MitSOX) induced by mitochondrial Ca2+ and Zn2+. The study aimed to investigate the effects of CSP and MLT combination on DBTRG-05MG cells, including cell viability, cell death, apoptosis, and MitSOX levels.
Article
Neurosciences
Kenan Yildizhan, Mustafa Naziroglu
Summary: TRPM2 channel is activated by excessive generation of mitochondrial and cytosolic ROS and influx of intracellular free Ca2+. The inhibitions of NMDA receptor and TRPM2 channel decrease ROS and apoptosis in neurons. The modulator role of memantine and NMDA via modulation of TRPM2 on oxidative neurodegeneration and apoptosis in SH-SY5Y cells was investigated. Inhibition of NMDA receptor via memantine treatment modulated HYP-mediated ROS, apoptosis, and TRPM2-induced excessive Ca2+ and may provide a strategy for protecting HYP-mediated neurodegenerative diseases.
Article
Obstetrics & Gynecology
Yusuf Dal, Mustafa Naziroglu, Mehmet Okan Ozkaya
Summary: This study aimed to investigate the effects of low molecular weight heparin (LMWH) on calcium signaling, oxidative toxicity, and apoptosis in the thrombocytes of recurrent pregnancy loss (RPL) patients via modulation of TRPM2 and TRPV1 channels. The results showed that the concentrations of [Ca2+] (i), cytROS, mitochondrial membrane potential, apoptosis, caspase-3, and caspase-9 were high in the plasma and thrombocytes of RPL patients, but were diminished by the treatments of LMWH, TRPM2, and TRPV1 channel blockers.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2023)