Cloning of Pseudomonas aeruginosa formaldehyde dehydrogenase gene into duckweed for future methane phytoremediation

Kok Sing Yee · Jan 18, 2024

This presentation shows a research idea in addressing global warming caused by methane emission. This research suggests the use of phytoremediation to reduce methane gas emission by cloning methanotrophs methane metabolizing gene into high-biomass plants such as duckweed. It offers greater benefits over conventional methane mitigation techniques as it is sustainable, and environmentally friendly and potentially more efficient. Duckweed was selected as the targeted host due to its rapid growth and adaptability, thus enhancing the feasibility of this approach. Transgenic duckweed can be planted in paddy fields or as cattle industry to reduce methane emissions as these sectors are found to release high amount of methane into the atmosphere. This research will be conducted by first isolating fdhA gene from P. aeruginosa, and subsequently construct it into an expression cassette. This expression cassette is further transformed into duckweed using Agrobacterium-mediated plant transformation
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