Fenugreek-based exosome like nanoparticles: A point of care vehicle for alleviating the pain intensity produced at primary dysmenorrheal condition in female of child bearing age

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Authors

Amoolya Sree¹ , Tamilvanan Shunmugaperumal¹

1. NIPER, Guwahati

Conference / event

Exotech, October 2022 (Virtual)

Poster summary

Fenugreek-based exosome like-nanoparticles (FEN-ELN) was prepared as a point of care vehicle for alleviating the pain intensity produced at primary dysmenorrheal condition. FEN-ELN, generated using polymer precipitation technique was screened to study the critical attributes which are likely to affect the quality and yield. Further, several characterization studies (size, zeta potential, trigonelline content, etc.) were conducted to evaluate the generated FEN-ELN. The successfully isolated FEN-ELN had an average diameter of 122.2 nm, negative surface charge of −21.9 mV and a concentration of 8.3 × 1010 particles/mL. Both the total protein and RNA concentration were deemed satisfactory.

Keywords

Exosome like-nanoparticles, Fenugreek, Primary dysmenorrhea, RP-HPLC

Research areas

Pharmacy and Pharmacology

References

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2. 2. Kim, J., Lee, Y. H., Wang, J., Kim, Y. K., & Kwon, I. K. (2022). Isolation and characterization of ginseng-derived exosome-like nanoparticles with sucrose cushioning followed by ultracentrifugation. SN Applied Sciences, 4(2), 63.
3. 3. Kalarikkal, S. P., Prasad, D., Kasiappan, R., Chaudhari, S. R., & Sundaram, G. M. (2020). A cost-effective polyethylene glycol-based method for the isolation of functional edible nanoparticles from ginger rhizomes. Scientific reports, 10(1), 1-12.

Funding

No data provided

Supplemental files

1. FEN-ELN formation checked by Nanoparticle Tracking Analyzer   Download

Additional information

Competing interests

No competing interests were disclosed.

Data availability statement

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

Creative Commons license

Copyright © 2023 Sree et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.