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Molecular characterization of gut microbiome of fasciolosis infected cattle with comparison to healthy cattle

PUBLISHED March 15, 2023 (DOI: https://doi.org/10.54985/peeref.2303p1793037)



Hafiz Muhammad Ishaq1 , Muhammad Usman1 , Sarmad Farogh Arshad1
  1. MNS-University of Agriculture, Multan Pakistan

Conference / event

International Conference on Sustainable Bioeconomy in Livestock and Crop Production, October 2021 (Multan, Pakistan)

Poster summary

Parasitic infestation fasciolosis affect the health of dairy animals as well as decrease the milk production. It has been hypothesized that there is gut microbial dysbiosis in fasciolosis. For a comparative analysis, a total of forty samples were collected (n = 20) from fasciolosis patients and (n = 20) from healthy ones. Fecal bacterial DNA was extracted and amplified V3+V4 region of 16S rRNA gene by using the primers. High through-put sequencing was performed for the characterization of gut microbiota. Compared to healthy animals, regarding the genera, Bifidobacterium and Shigella were significantly higher while Prevotella_9 and Dialister in were sufficiently low. Moreover, the species analysis showed a significantly high abundance of Prevotella copri and Escherichia coli in Fasciolosis group. The results of current study are aligned with proposed hypothesis of gut bacterial dysbiosis in fasciolois. The findings of the current study provided a basic foundation to understand gut microbiota in treatment of fasciolosis, disease pathway, mechanism and probiotic development


Fasciolosis, Gut microbiota, High-throughput, Dysbiosis

Research areas

Veterinary and Animal Sciences


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Supplemental files

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Additional information

Competing interests
No competing interests were disclosed.
Data availability statement
The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.
Creative Commons license
Copyright © 2023 Ishaq et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ishaq, H., Usman, M., Arshad, S. Molecular characterization of gut microbiome of fasciolosis infected cattle with comparison to healthy cattle [not peer reviewed]. Peeref 2023 (poster).
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