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Molecular characterization of gut microbiome of fasciolosis infected cattle with comparison to healthy cattle
发表日期 March 15, 2023 (DOI: https://doi.org/10.54985/peeref.2303p1793037)
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作者
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Hafiz Muhammad Ishaq1 , Muhammad Usman1 , Sarmad Farogh Arshad1
- MNS-University of Agriculture, Multan Pakistan
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会议/活动
- International Conference on Sustainable Bioeconomy in Livestock and Crop Production, October 2021 (Multan, Pakistan)
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海报摘要
- Parasitic infestation fasciolosis affect the health of dairy animals as well as decrease the milk production. It has been hypothesized that there is gut microbial dysbiosis in fasciolosis. For a comparative analysis, a total of forty samples were collected (n = 20) from fasciolosis patients and (n = 20) from healthy ones. Fecal bacterial DNA was extracted and amplified V3+V4 region of 16S rRNA gene by using the primers. High through-put sequencing was performed for the characterization of gut microbiota. Compared to healthy animals, regarding the genera, Bifidobacterium and Shigella were significantly higher while Prevotella_9 and Dialister in were sufficiently low. Moreover, the species analysis showed a significantly high abundance of Prevotella copri and Escherichia coli in Fasciolosis group. The results of current study are aligned with proposed hypothesis of gut bacterial dysbiosis in fasciolois. The findings of the current study provided a basic foundation to understand gut microbiota in treatment of fasciolosis, disease pathway, mechanism and probiotic development
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关键词
- Fasciolosis, Gut microbiota, High-throughput, Dysbiosis
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研究领域
- Veterinary and Animal Sciences
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参考文献
- 暂无数据
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基金
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补充材料
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附加信息
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- 利益冲突
- No competing interests were disclosed.
- 数据可用性声明
- The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.
- 知识共享许可协议
- Copyright © 2023 Ishaq et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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引用
Ishaq, H., Usman, M., Arshad, S. Molecular characterization of gut microbiome of fasciolosis infected cattle with comparison to healthy cattle [not peer reviewed]. Peeref 2023 (poster).
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