Chemical modified natural nanogels crosslinked with S Benzyl L cysteine exhibit potent antibacterial activity

---

Authors

Fang-Yu Chung¹ , Yu-Fon Chen²

1. Department of Chemical Engineering, National Cheng Kung University
2. National Taitung University

Conference / event

Annual Meeting of the Polymer Society, January 2023 (Chang Gung University, Taiwan)

Poster summary

Improper use of antibiotics has accelerated the global rise of drug-resistant bacteria, especially strains that form biofilms. With the goal of achieving good biocompatibility while reducing adverse effects, we extracted nucleic acid from kiwifruit and used it as a molecular backbone. After HCl treatment, the DNA increased its opened deoxylriboses. S-Benzyl-L-cysteine (SBLC) was then crosslinked with the aldehyde groups in DNA under emulsion processes, becoming SBLC-modified DNA-HCl nanogels. These natural NGs modified with hydrophobic SBLC were analyzed for antibacterial activities. In cell viability assay, these modified natural NGs revealed low cytotoxicity toward normal cells. In antibacterial assays, these modified natural NGs inhibited bacteria, especially biofilms-formed P. aeruginosa and pathogenic E. coli, while exhibiting bacterial membrane rupture as seen through SEM and ameliorating P. aeruginosa-induced lethality in C. elegans. These modified natural NGs may provide a novel anti-biofilm agent in combating resistant bacterial infection.

Keywords

Natural nanogel, S-Benzyl-L-cysteine, Drug-resistant bacteria, Anti-biofilm agent

Research areas

Chemical Engineering, Microbiology, Nanoengineering, Material Sciences

References

1. ZnO-loaded DNA nanogels as neutrophil extracellular trap-like structures in the treatment of mouse peritonitis. Materials Science and Engineering C 2021, 131, 112484.
2. Effect of oil-water interface and payload-DNA interactions on payload-encapsulated DNA nanogels. Journal of Polymer Research 2022, 29, 8.
3. Naturally derived DNA nanogels as pH- and glutathione-triggered anticancer drug carriers. Materials Science and Engineering C 2020, 114, 111025.

Funding

1. National Science and Technology Council (No. MOST 111 2635 E 143 001)

Supplemental files

No data provided

Additional information

Competing interests

No competing interests were disclosed.

Data availability statement

Data sharing not applicable to this poster as no datasets were generated or analyzed during the current study.

Creative Commons license

Copyright © 2023 Chung et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.