Article
Food Science & Technology
Faidra Syropoulou, Foteini F. Parlapani, Stefanos Kakasis, George-John E. Nychas, Ioannis S. Boziaris
Summary: Primary processing and storage impact the microbial communities of gutted and filleted fish compared to whole fish. High Resolution Sequencing analysis can differentiate bacteria isolated from fish products and reveal contamination due to handling and processing, assisting stakeholders in addressing issues related to fish microbial quality or safety promptly.
Article
Microbiology
Faidra Syropoulou, Dimitrios A. Anagnostopoulos, Foteini F. Parlapani, Evangelia Karamani, Anastasios Stamatiou, Kostas Tzokas, George-John E. Nychas, Ioannis S. Boziaris
Summary: This study examined the bacterial communities of whole and filleted European sea bass under different storage temperatures and atmosphere conditions. The results showed that handling, temperature, and atmosphere conditions strongly influenced the shelf-life of the fish. Pseudomonas was found to be the dominant bacteria throughout most of the storage period, but there were variations in the microbiota profile at different stages of storage. The study provides valuable information for developing strategies to extend the shelf-life of sea bass.
Article
Fisheries
Socorro Toxqui-Rodriguez, Fernando Naya-Catala, Ariadna Sitja-Bobadilla, M. Carla Piazzon, Jaume Perez-Sanchez
Summary: This study compared different sequencing platforms for microbial characterization in fish gut mucosal samples. The results showed that MinION is a reliable and accurate tool for assessing intestinal bacteria communities, with good correlation with Illumina results. However, less abundant but biologically important taxa may be masked compared to Illumina sequencing.
Article
Biotechnology & Applied Microbiology
Aurelien Maillet, Agnes Bouju-Albert, Steven Roblin, Pauline Vaissie, Sebastien Leuillet, Xavier Dousset, Emmanuel Jaffres, Jerome Combrisson, Herve Prevost
Summary: Amplicon sequencing was used to analyze bacterial communities associated with cold-smoked salmon products and processing plant surfaces. The study found that DNA extraction methods influenced the observed microbiota composition in cold-smoked salmon, while cleaning and disinfection procedures affected surface microbiota richness and diversity. Genus-level analysis identified dominant bacteria and beta-diversity analyses indicated that surface topology and sampling methods affected bacterial community composition. Standardization of methodology and analytical validation are important for accurate 16S rDNA metabarcoding surveys.
Article
Fisheries
Maria F. Morales-Rivera, Diego Valenzuela-Miranda, Valentina Valenzuela-Munoz, Gustavo Nunez-Acuna, Ruben Avendano-Herrera, Cristian Gallardo-Escarate
Summary: This study aimed to characterize the bacterial community associated with Caligus rogercresseyi in Chilean salmon aquaculture. It found that sea lice can act as potential reservoirs for fish diseases in the aquatic environment.
Article
Fisheries
Lucsame Gruneck, Vasana Jinatham, Phatthanaphong Therdtatha, Siam Popluechai
Summary: This study investigated the differences in gut microbiota profiles of healthy Siamese fighting fish at different life stages. The results showed significant differences in overall bacterial profiles between young and adult fish, as well as changes in bacterial diversity with age. The study also identified variations in the abundance of Proteobacteria phylum members and their predicted functions between young and adult fish, suggesting their potential role in improving fish growth. These findings contribute to a better understanding of the role of gut microbiota and its association with host factors.
Article
Biotechnology & Applied Microbiology
T. P. R. A. Legrand, M. L. Wos-Oxley, J. W. Wynne, L. S. Weyrich, A. P. A. Oxley
Summary: This study evaluated the microbial viability of fish gut microbiota in digesta and mucosal samples using a modified PMA protocol and 16S rRNA gene sequencing. The findings suggest that important bacterial members may not be active constituents in the fish gut microbiota, and both sample type and PMA treatment influence the bacterial communities found in the fish gut microbiota.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Fisheries
Olga Burtseva, Anna Kublanovskaya, Tatiana Fedorenko, Elena Lobakova, Konstantin Chekanov
Summary: This study revealed the previously undescribed bacterial diversity in the White Sea fish gut microbiota using 16S rRNA metabarcoding, showing specific patterns of bacteria common in the water of this region.
Article
Environmental Sciences
Aoyun Li, Yajing Wang, Yuanyuan He, Bingxian Liu, Mudassar Iqbal, Khalid Mehmood, Tariq Jamil, Yung-Fu Chang, Lianmei Hu, Ying Li, Jianying Guo, Jiaqiang Pan, Zhaoxin Tang, Hui Zhang
Summary: Fluorine and its compounds from industrial production and coal combustion can cause contamination of air, water, and soil, posing a threat to public health through accumulation in animals, plants, and humans via food chain. Exposure to fluoride resulted in damaged intestinal structure and altered gut microbial composition in ducks.
Article
Biotechnology & Applied Microbiology
Shoichiro Kameoka, Daisuke Motooka, Satoshi Watanabe, Ryuichi Kubo, Nicolas Jung, Yuki Midorikawa, Natsuko O. Shinozaki, Yu Sawai, Aya K. Takeda, Shota Nakamura
Summary: The study compared 192 Japanese volunteers' fecal 16S analysis data using modified V1-V2 and standard V3-V4 primers. Results showed that the bacterial composition derived from the V34 region might differ from the actual abundance, suggesting that the modified V12 primer set is more desirable for analyzing the Japanese gut microbiota.
Article
Microbiology
Lucia Diaz-Abad, Natassia Bacco-Mannina, Fernando Miguel Madeira, Ester A. Serrao, Aissa Regalla, Ana R. Patricio, Pedro R. Frade
Summary: The study characterized the gut microbiome of green sea turtles and their food items, finding Proteobacteria, Bacteroidetes, and Firmicutes as the most abundant bacterial phyla. Two red algae bacterial indicators were also identified in the cloacal compartments, suggesting the ingestion of microbial components that assist in digesting these food items.
Article
Neurosciences
Wei Cui, Li Xu, Lin Huang, Yang Tian, Yan Yang, Yamei Li, Qian Yu
Summary: The study aims to explore the characteristics of gut microbiota changes over time after stroke. The researchers compared the clinical data and gut microbiota of stroke patients and healthy subjects in two phases. The results showed that gut dysbiosis still existed in patients in the subacute phase and convalescent phase of stroke, and gradually improved with the recovery of stroke. There is a strong correlation between gut microbiota and cognitive function after stroke.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Medicine, Research & Experimental
Rui-zhi Yang, Min Liang, Song Lin, Jing Weng, Jia-min Hu, Shi-zhu Lin, Xiao-dan Wu, Kai Zeng
Summary: This study investigated the effect of general anesthetics on the lung microbiome and found that continuous inhalation of isoflurane altered the diversity and composition of the lung microbiota in rats, resulting in post-anesthesia depression.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo
Summary: Accessibility to next-generation sequencing (NGS) has enabled the profiling of microbial communities. Choosing the right 16S rRNA hypervariable region for sequencing is critical. This study evaluated the performance of different hypervariable regions in male urinary microbiota profiling and found that V1V2 region is more suitable.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shuangshuang Wang, Feng Song, Haoyu Gu, Zhilong Shu, Xiaowen Wei, Ke Zhang, Yuxiang Zhou, Lanrui Jiang, Zefei Wang, Jienan Li, Haibo Luo, Weibo Liang
Summary: This study investigated the gut microbiota of healthy adults with different traits. The results showed differences in gut microbiota composition between male and female subjects. Additionally, the gut microbiota composition was able to distinguish between different BMI groups. At the phylum level, specific phyla were found to be increased in different BMI groups for both males and females. At the genus level, several distinct genera were identified across BMI groups. The accuracy of predicting body type using a linear regression model was 74%.
MICROBIAL CELL FACTORIES
(2022)
Article
Food Science & Technology
Dimitrios A. Anagnostopoulos, Foteini F. Parlapani, Ioannis S. Boziaris
Summary: The modern advances in technology have transformed the study of seafood microbiota, enhancing our understanding in this field. Utilizing modern tools to explore and understand the complexities of seafood spoilage is crucial for improving seafood quality and ensuring value along the seafood value chain.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
S. I. Ekonomou, F. F. Parlapani, M. Kyritsi, C. Hadjichristodoulou, I. S. Boziaris
Summary: This study monitored the storage of vacuum-packed hot smoked rainbow trout fillets from two different smokehouses in Greece at different temperatures and observed changes in microbiology. The shelf-life of the products differed between the two smokehouses. The study also identified and classified microbial communities grown at different pH levels.
Article
Environmental Sciences
Maria Demertzioglou, Savvas Genitsaris, Antonios D. Mazaris, Aris Kyparissis, Dimitra Voutsa, Argyri Kozari, Konstantinos Ar Kormas, Natassa Stefanidou, Matina Katsiapi, Evangelia Michaloudi, Maria Moustaka-Gouni
Summary: The investigation in a European protected wetland revealed that changes in phytoplankton and zooplankton dynamics, particularly the collapse of harmful blooms, led to a mass kill of fauna. High nutrient concentrations and inhibitory ammonia levels played a key role in triggering the collapse of harmful blooms, ultimately causing a shift in the ecosystem dynamics.
ENVIRONMENTAL POLLUTION
(2022)
Article
Agronomy
Evangelia Tsoumalakou, Eleni Mente, Konstantinos A. Kormas, Nikolaos Katsoulas, Nikolaos Vlahos, Panagiotis Kapsis, Efi Levizou
Summary: This study examines the impact of nutrient deficiencies on plant growth in aquaponics, finding that supplementing with iron and iron combined with potassium can increase lettuce and red tilapia production. The results emphasize the importance of monitoring crop functional responses for sustainable aquaponics.
Article
Food Science & Technology
Dimitrios A. Anagnostopoulos, Faidra Syropoulou, Foteini F. Parlapani, Athanasios Tsiartsafis, Athanasios Exadactylos, George-John E. Nychas, Ioannis S. Boziaris
Summary: The microbiota profile of gilthead seabream fillets stored under different conditions was studied using 16S rRNA metabarcoding sequencing. The results showed that storage conditions and temperature had a significant impact on the shelf-life of the fillets. Pseudomonas was the dominant genus at the end of the fillets' shelf-life, but other genera like Shewanella and Carnobacterium were also present in the middle stages of MAP-stored fillets.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Ecology
Konstantinos Kormas, Eleni Nikouli, Vasiliki Kousteni, Dimitrios Damalas
Summary: Fish microbiome science is progressing fast, but research on natural fish populations is lacking. This study analyzed the midgut bacterial microbiota of 12 fish species from the Gyaros Island marine protected area and found that the diversity of gut bacteria was significant and varied among different fish species.
Article
Microbiology
Foteini F. Parlapani, Dimitrios A. Anagnostopoulos, Evangelia Karamani, Athanasios Mallouchos, Serkos A. Haroutounian, Ioannis S. Boziaris
Summary: Microbial spoilage is the main reason for quality deterioration in seafood, particularly due to psychotropic Pseudomonas strains that produce volatile organic compounds (VOC). This study investigated the growth and VOC production of seven Pseudomonas strains associated with spoiled fish on model fish substrate at different storage temperatures. The results showed strain-dependent VOC profiles and revealed potential Chemical Spoilage Indices (CSI). This research contributes to understanding the metabolic activity of specific Pseudomonas strains and provides potential CSI for rapid evaluation of fish spoilage/freshness status.
Article
Fisheries
Nikolaos Vlahos, Efi Levizou, Efstathia Patsea, Kalliopi Tasiou, Panagiotis Berillis, Efthimia Antonopoulou, Vlasoula Bekiari, Natalia Martou, Konstantinos Morfesis, Diamanto Lazari, Christodoulos-Stefanos Chatzimavroudis, Nikos Krigas, Konstantinos Kormas, Elena Mente
Summary: Brackish aquaponics using euryhaline fish and halophyte plants have potential as sustainable method for functional food production and high nutritional, commercial, and pharmaceutical value. The study found that sea bass exhibited better growth performance and feed conversion rate at 8 ppt and 14 ppt salinity levels compared to 20 ppt, while rock samphire showed better growth and survival at 8 ppt. Nitrate and phosphorous removal efficiency were higher at 8 ppt and 14 ppt, and essential oil yield of rock samphire was highest at 14 ppt. Overall, this research demonstrates the satisfactory growth performance of sea bass and rock samphire in a tailored brackish aquaponic system.
Article
Chemistry, Multidisciplinary
Nikolaos Vlahos, Panagiotis Berillis, Efi Levizou, Efstathia Patsea, Nikolas Panteli, Maria Demertzioglou, Konstantinos Morfesis, Georgia Voudouri, Nikos Krigas, Kostantinos Kormas, Efthimia Antonopoulou, Eleni Mente
Summary: This study investigated the effect of different salinities on the growth performance and survival rate of sea bream and rock samphire in a brackish aquaponic system. The results showed that salinity 8 ppt had the best growth performance for both sea bream and rock samphire. Exposure to different salinities also resulted in changes in the expression of Hsps and MAPKs.
APPLIED SCIENCES-BASEL
(2023)
Article
Microbiology
Jonathan Rhoades, Stamatia Fotiadou, Georgia Paschalidou, Theodoti Papadimitriou, Avelino Alvarez Ordonez, Konstantinos Kormas, Elisabeth Vardaka, Eleni Likotrafiti
Summary: This study examined the microbial population and presence of cyanobacterial toxins in commercially available spirulina products. Potentially pathogenic bacteria were detected in the products, along with microcystin toxins at levels that could exceed the recommended daily limits. The study highlights microbiological safety issues associated with commercial spirulina products produced in open ponds.
Article
Food Science & Technology
Dimitrios A. Anagnostopoulos, Foteini F. Parlapani, Evangelia Tsara, Maria G. Eirinaki, Despoina Kokioumi, Evdoxia Ampatzidou, Ioannis S. Boziaris
Summary: The aim of this study was to evaluate the effect of hurdles such as a(w) and pH, as well as storage atmosphere, on the microbiological and sensory changes of minimally processed European sea bass fillets. The results showed that the shelf-life of brined fillets stored aerobically was 6 days, while vacuum and MAP storage extended it to 12 days. The use of acetic or citric acid as marinades also increased the shelf-life, with acetic acid achieving 30 and 40 days under aerobic and reduced oxygen conditions, respectively.
Article
Agriculture, Dairy & Animal Science
Nikolas Panteli, Konstantinos Feidantsis, Maria Demertzioglou, Vasiliki Paralika, Stelios Karapanagiotis, Constantinos C. Mylonas, Konstantinos Ar. Kormas, Eleni Mente, Pavlos Makridis, Efthimia Antonopoulou
Summary: Probiotic treatment with Phaeobacter inhibens enhances growth and development of greater amberjack through modulation of cellular signaling pathways involved in metamorphosis. The probiotic treatment promotes protein synthesis and hypertrophic growth, while reducing MAPKs levels. It also induces apoptosis and autophagy for structural remodeling processes, and increases enzymatic activities for energy demands of metamorphosis. These findings demonstrate the importance of probiotics in enhancing fish development and growth.
Article
Microbiology
Dimitris Rigas, Nikos Grivas, Aikaterini Nelli, Evangelia Gouva, Ioannis Skoufos, Konstantinos Kormas, Athina Tzora, Ilias Lagkouvardos
Summary: The use of antibiotics in open-water aquaculture can affect the growth of fish by disrupting the gut microbiota and increasing the abundance of parasites. This study highlights the need for interventions to restore a healthy and protective gut microbiome.
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.