Article
Biotechnology & Applied Microbiology
Juhong Chen, Wenzhao Xu, Zi Wang, Li Tian, Weidong Liu, Ang Ren, Rui Liu, Jing Zhu, Liang Shi
Summary: Mitochondrial pyruvate carriers play a crucial role in regulating the biosynthesis of ganoderic acid in Ganoderma lucidum.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Da-Wei Li, Min Liu, Yue-Qi Leng, Jin-Fang Hu, Sa Deng, Ai-Jing Leng, Xiao-Chi Ma, Ruo-Yu Wang, Jian Zhou, Chao Wang
Summary: This study investigated the bioactive phytochemicals in Ganoderma lucidum and found that its triterpenoids showed inhibitory effects on the enzyme FAAH, indicating their potential for treating neuroinflammation.
Article
Plant Sciences
Xuelian Zhang, Daoshun Wu, Yu Tian, Xiangdong Chen, Jin Lan, Fei Wei, Ye Li, Yun Luo, Xiaobo Sun
Summary: The study found that Ganoderma lucidum polysaccharides (GLP) have a protective role in LPS-induced acute pneumonia. GLP acts through multiple mechanisms, including blocking inflammatory cell infiltration, inhibiting cytokine secretion, suppressing Neuropilin-1 (NRP1) activation, and regulating pneumonocyte apoptosis and autophagy.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Loreto Robles-Hernandez, Nora A. Salas-Salazar, Ana C. Gonzalez-Franco
Summary: This study aimed to purify and characterize the antibacterial activity against plant pathogenic bacteria from culture fluids of Ganoderma lucidum, revealing that the bioactive compounds are small polysaccharides. This is the first report of such polysaccharides produced by G. lucidum with activity against bacterial plant pathogens.
Article
Agricultural Engineering
Xiao-Ting Zhen, Yan Chen, Ya-Ling Yu, Min-Zhen Shi, Tian-Ci Yan, Zi-Xuan Yue, Yu-Xin Gu, Hui Zheng, Jun Cao
Summary: This study developed a simple, green, and low-consumption method for the extraction of ganoderic acids. The effects of different parameters on extraction efficiency were investigated, resulting in the highest yields achieved under specific conditions.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Mohd Hafizur Rehman Ansari, Washim Khan, Rabea Parveen, Sadia Saher, Sayeed Ahmad
Summary: This study aimed to prepare a triterpenoid enriched fraction (TEF) containing ganoderic acid H (GA-H) from Ganoderma lucidum and evaluate its pharmacokinetics, metabolomics, and stability. The results showed that TEF contained bioavailable constituents and remained stable at room temperature for up to one year. This suggests that the GA-H-enriched TEF can be explored as phytopharmaceuticals for different pharmacological properties.
Article
Biochemistry & Molecular Biology
Xiao-Cui Li, Fei Liu, Hai-Guo Su, Cheng Peng, Qin-Mei Zhou, Juan Liu, Yuan-Jin Huang, Li Guo, Liang Xiong
Summary: This study isolated twelve previously undescribed lanostane-type triterpene acids from Ganoderma luteomarginatum, with some compounds showing significant cytotoxicity against human cancer cells. The comparison with previous literature revealed that ganoderic alcohols have stronger cytotoxicity than ganoderic acid derivatives in the genus Ganoderma.
Article
Biotechnology & Applied Microbiology
Wen-Fang Wang, Han Xiao, Jian-Jiang Zhong
Summary: In this study, a new GA, DHLDOA, was successfully synthesized in engineered yeast by oxidation of HLDOA using CYP5139G1 from Ganoderma lucidum. Enzymatic experiments confirmed the activity of CYP5139G1, and strategies to increase DHLDOA production were also explored.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biology
Rui Liu, Ting Zhu, Xin Chen, Zi Wang, Zhengyan Yang, Ang Ren, Liang Shi, Hanshou Yu, Mingwen Zhao
Summary: This study reveals that GSNOR regulates secondary metabolite production in microorganisms through S-nitrosylation of target proteins, specifically catalase. The findings shed light on the physiological functions of S-nitrosylated proteins and provide insights into the mechanism by which GSNOR responds to stress.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Analytical
Livia Oliveira Alves Ferreira, Szilard Molnar, Felipe Terra Martins, Pal Perjesi, Luiz Henrique Keng Queiroz, Caridad Noda-Perez
Summary: The study on symmetrical halogenated bischalcones (SHBCs) and their Michael addition reaction with reduced glutathione (GSH) revealed that the formation of adducts did not directly correlate with their cytotoxic effects on cancer cells.
MICROCHEMICAL JOURNAL
(2021)
Article
Microbiology
Li Meng, Ruyue Zhou, Jialong Lin, Xizhe Zang, Qingji Wang, Panmeng Wang, Li Wang, Zhuang Li, Wei Wang
Summary: Transcriptome and metabolome analyses were conducted to identify transcription factors involved in ganoderic acid (GA) biosynthesis during development of Ganoderma lucidum. It was found that the biosynthesis of GAs and flavonoids significantly changed during fungal development, with 53 potential transcription factor families identified, including homeobox transcription factor and velvet family protein that played important roles in GA biosynthesis. The results are expected to enhance understanding of secondary metabolite biosynthesis and development in fungi.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yun-Li Peng, Yong-Xiang Wang, Yong-Xian Cheng
Summary: The Ganoderma lucidum mushroom, a traditional medicine in China for over 2000 years, was found to contain five undescribed minor meroterpenoids, baoslingzhines F-J (1-5), which possess dihydropyran moiety. Spectroscopic and computational methods were used to determine their structures and stereochemistry. Chiral HPLC separation of the (+)- and (-)-enantiomers was performed. Both enantiomers of baoslingzhines F-J were found to inhibit the expression of fibronectin, collagen I, and alpha-SMA, proteins related to renal fibrosis, in TGF-β1-induced rat kidney proximal tubular cells.
Article
Biochemical Research Methods
Yun Ling, Qing Zhang
Summary: The bark of Ilex rotunda has been used as a traditional Chinese medicine with triterpene saponins (TSs) being the major bioactive ingredients. A method using HPLC-ESI-QTOF-MS/MS was established to identify 26 TSs in BIr, with 23 of them being reported for the first time. This study provides a comprehensive investigation on TSs in BIr, laying the foundation for its further development and utilization.
PHYTOCHEMICAL ANALYSIS
(2021)
Review
Biochemical Research Methods
Mirela Sarbu, Raluca Ica, Alina D. Zamfir
Summary: Gangliosides, abundant in the nervous system, are useful biomarkers for diagnosing NS diseases. This review discusses the use of separation techniques and MS in analyzing gangliosides, particularly microfluidics MS and ion mobility separation MS for studying brain gangliosidome in neurodegenerative diseases and brain cancer. High-performance techniques in ganglioside research are beneficial and offer promising perspectives.
Article
Microbiology
Tao Wu, Jiale Xia, Feng Ge, Hao Qiu, Li Tian, Xiaotian Liu, Rui Liu, Ailiang Jiang, Jing Zhu, Liang Shi, Hanshou Yu, Mingwen Zhao, Ang Ren
Summary: Putrescine (Put) plays a regulatory role in cell growth, and its regulation in fungi, particularly in the biosynthesis of ganoderic acid (GA), involves interactions with ornithine decarboxylase (ODC) proteins and the antizyme (AZ) protein. The target of rapamycin (TOR) can promote Put accumulation and affect GA biosynthesis by inhibiting the level of AZ protein. This study provides insights into the regulatory mechanisms of Put and secondary metabolism in fungi.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Applied
Roya Moghimi, Lida Ghaderi, Hasan Rafati, Atousa Aliahmadi, David Julian McClements
Article
Food Science & Technology
Roya Moghimi, Atousa Aliahmadi, David Julian McClements, Hasan Rafati
LWT-FOOD SCIENCE AND TECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
L. Ghaderi, R. Moghimi, A. Aliahmadi, D. J. McClements, H. Rafati
JOURNAL OF APPLIED MICROBIOLOGY
(2017)
Article
Acoustics
Roya Moghimi, Atousa Aliahmadi, Hasan Rafati
ULTRASONICS SONOCHEMISTRY
(2017)
Article
Chemistry, Physical
Roya Moghimi, Atousa Aliahmadi, Hasan Rafati, Hamid Reza Abtahi, Shahideh Amini, Mohamad Mehdi Feizabadi
JOURNAL OF MOLECULAR LIQUIDS
(2018)
Article
Biochemistry & Molecular Biology
Somayeh Keypour, Hossein Riahi, Naser Safaie, Ali Borhani
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
(2014)
Article
Food Science & Technology
Zeinab Mazarei, Hasan Rafati
LWT-FOOD SCIENCE AND TECHNOLOGY
(2019)
Article
Plant Sciences
Somayeh Keypour, Mohammad Reza Asef
AUSTRALASIAN PLANT PATHOLOGY
(2020)
Article
Forestry
Somayeh Keypour, Hossein Riahi, Mohammad Reza Asef, Jafar Abdollahzadeh, Ali Borhani, Naser Safaie
Article
Chemistry, Medicinal
K. Habibi, H. Sepehri, L. Delphi, M. H. Mirjalili, H. Rafati
Article
Chemistry, Applied
Roya Moghimi, Atousa Aliahmadi, Hasan Rafati
CARBOHYDRATE POLYMERS
(2017)
Article
Pharmacology & Pharmacy
Parisa Seyedemadi, Mehdi Rahnema, Mohammad Reza Bigdeli, Shahrebano Oryan, Hassan Rafati
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2016)
Meeting Abstract
Public, Environmental & Occupational Health
Pardis Irankhahi, Hossein Riyahi, Alireza Ghassem Pour, Farzaneh Zandi, Parisa Savedoroudi, Somayeh Keypour, Saeed Ali Mousazadeh
IRANIAN JOURNAL OF PUBLIC HEALTH
(2016)
Article
Chemistry, Medicinal
H. Rafati, F. Mirzajani
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.