Intrinsically disordered proteins in crowded milieu: when chaos prevails within the cellular gumbo

Labeling for Quantitative Comparison of Imaging Measurements in Vitro and in Cells

(2018) Caitlin M. Davis et al.   BIOCHEMISTRY

Convergence of Artificial Protein Polymers and Intrinsically Disordered Proteins

(2018) Michael Dzuricky et al.   BIOCHEMISTRY

In Aqua Veritas: The Indispensable yet Mostly Ignored Role of Water in Phase Separation and Membrane-less Organelles

(2018) Boris Y. Zaslavsky et al.   BIOCHEMISTRY

Disorder-to-order transitions in the molten globule-like Golgi Reassembly and Stacking Protein

(2018) Luís F.S. Mendes et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Intrinsically Disordered Protein Exhibits Both Compaction and Expansion under Macromolecular Crowding

(2018) Anthony Banks et al.   BIOPHYSICAL JOURNAL

Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation

(2018) Veronica H. Ryan et al.   MOLECULAR CELL

Protein plasticity driven by disorder and collapse governs the heterogeneous binding of CytR to DNA

(2018) Sneha Munshi et al.   NUCLEIC ACIDS RESEARCH

Dual roles for ATP in the regulation of phase separated protein aggregates in Xenopus oocyte nucleoli

(2018) Michael H Hayes et al.   eLife

Extreme disorder in an ultrahigh-affinity protein complex

(2018) Alessandro Borgia et al.   NATURE

Role of solvent properties of water in crowding effects induced by macromolecular agents and osmolytes

(2017) L. A. Ferreira et al.   Molecular BioSystems

Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity

(2017) Joseph R. Simon et al.   Nature Chemistry

Direct observation of structure and dynamics during phase separation of an elastomeric protein

(2017) Sean E. Reichheld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

(2017) Jacob P. Brady et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Soft interactions and volume exclusion by polymeric crowders can stabilize or destabilize transient structure in disordered proteins depending on polymer concentration

(2017) Farai I. Rusinga et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Intrinsically Disordered Proteome of Human Membrane-Less Organelles

(2017) April L. Darling et al.   PROTEOMICS

ATP controls the crowd

(2017) Allyson M. Rice et al.   SCIENCE

ATP as a biological hydrotrope

(2017) Avinash Patel et al.   SCIENCE

Liquid–liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein Tau

(2017) Susmitha Ambadipudi et al.   Nature Communications

Globular Protein Folding In Vitro and In Vivo

(2016) Martin Gruebele et al.   Annual Review of Biophysics

ReAsH as a Quantitative Probe of In-Cell Protein Dynamics

(2016) Hannah Gelman et al.   BIOCHEMISTRY

Protein Composition Determines the Effect of Crowding on the Properties of Disordered Proteins

(2016) Cayla M. Miller et al.   BIOPHYSICAL JOURNAL

Phase separation in biology; functional organization of a higher order

(2016) Diana M. Mitrea et al.   Cell Communication and Signaling

Macromolecular and Small Molecular Crowding Have Similar Effects on α-Synuclein Structure

(2016) Jia Bai et al.   CHEMPHYSCHEM

The Unstructured N-terminal Region ofArabidopsisGroup 4 Late Embryogenesis Abundant (LEA) Proteins Is Required for Folding and for Chaperone-like Activity under Water Deficit

(2016) Cesar L. Cuevas-Velazquez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A J-modulated protonless NMR experiment characterizes the conformational ensemble of the intrinsically disordered protein WIP

(2016) Eva Rozentur-Shkop et al.   JOURNAL OF BIOMOLECULAR NMR

Protein unfolding in crowded milieu: what crowding can do to a protein undergoing unfolding?

(2016) Olga V. Stepanenko et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Mechanistic insights into mammalian stress granule dynamics

(2016) Marc D. Panas et al.   JOURNAL OF CELL BIOLOGY

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Structural disorder of monomeric α-synuclein persists in mammalian cells

(2016) Francois-Xavier Theillet et al.   NATURE

Principles and Properties of Stress Granules

(2016) David S.W. Protter et al.   TRENDS IN CELL BIOLOGY

New structural insights into Golgi Reassembly and Stacking Protein (GRASP) in solution

(2016) Luís F. S. Mendes et al.   Scientific Reports

How can macromolecular crowding inhibit biological reactions? The enhanced formation of DNA nanoparticles

(2016) Sen Hou et al.   Scientific Reports

Folding of the Tau Protein on Microtubules

(2015) Harindranath Kadavath et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Nuclear bodies: the emerging biophysics of nucleoplasmic phases

(2015) Lian Zhu et al.   CURRENT OPINION IN CELL BIOLOGY

Real-time NMR monitoring of biological activities in complex physiological environments

(2015) Matthew J Smith et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Elastin-like polypeptides as models of intrinsically disordered proteins

(2015) Stefan Roberts et al.   FEBS LETTERS

Compartmentalization and Functionality of Nuclear Disorder: Intrinsic Disorder and Protein-Protein Interactions in Intra-Nuclear Compartments

(2015) Fanchi Meng et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Beyond the Excluded Volume Effects: Mechanistic Complexity of the Crowded Milieu

(2015) Irina Kuznetsova et al.   MOLECULES

Encoding phase transitions

(2015) Alex S. Holehouse et al.   NATURE MATERIALS

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells

(2015) Iwo König et al.   NATURE METHODS

Analysis of the size dependence of macromolecular crowding shows that smaller is better

(2015) Kim A. Sharp   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Selective observation of the disordered import signal of a globular protein by in-cell NMR: The example of frataxins

(2015) Matija Popovic et al.   PROTEIN SCIENCE

Hydrogen exchange of disordered proteins inEscherichia coli

(2015) Austin E. Smith et al.   PROTEIN SCIENCE

Molecular weight–gyration radius relation of globular proteins: a comparison of light scattering, small-angle X-ray scattering and structure-based data

(2015) Detlef-M. Smilgies et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Live Cell NMR

(2014) Darón I. Freedberg et al.   Annual Review of Biophysics

Folding of an Unfolded Protein by Macromolecular Crowding in Vitro

(2014) Jörgen Ådén et al.   BIOCHEMISTRY

Molecular Description of the LCST Behavior of an Elastin-Like Polypeptide

(2014) Nan K. Li et al.   BIOMACROMOLECULES

Minimal Effects of Macromolecular Crowding on an Intrinsically Disordered Protein: A Small-Angle Neutron Scattering Study

(2014) David P. Goldenberg et al.   BIOPHYSICAL JOURNAL

Conditionally and Transiently Disordered Proteins: Awakening Cryptic Disorder To Regulate Protein Function

(2014) Ursula Jakob et al.   CHEMICAL REVIEWS

Pathological Unfoldomics of Uncontrolled Chaos: Intrinsically Disordered Proteins and Human Diseases

(2014) Vladimir N. Uversky et al.   CHEMICAL REVIEWS

Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates

(2014) Vladimir N. Uversky et al.   FEBS LETTERS

Synthetic crowding agent dextran causes excluded volume interactions exclusively to tracer protein apoazurin

(2014) Alexander Christiansen et al.   FEBS LETTERS

What Macromolecular Crowding Can Do to a Protein

(2014) Irina Kuznetsova et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A Frustrated Binding Interface for Intrinsically Disordered Proteins

(2014) Per Jemth et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Assemblages: Functional units formed by cellular phase separation

(2014) Jeffrey A. Toretsky et al.   JOURNAL OF CELL BIOLOGY

Protein Folding Dynamics in the Cell

(2014) Irisbel Guzman et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Single-molecule spectroscopy reveals polymer effects of disordered proteins in crowded environments

(2014) Andrea Soranno et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The structural and functional signatures of proteins that undergo multiple events of post-translational modification

(2014) Vikas Pejaver et al.   PROTEIN SCIENCE

GRASP65 controls the cis Golgi integrity in vivo

(2014) T. Veenendaal et al.   Biology Open

Quantification of Excluded Volume Effects on the Folding Landscape of Pseudomonas aeruginosa Apoazurin In Vitro

(2013) Alexander Christiansen et al.   BIOPHYSICAL JOURNAL

Dual Specificity Kinase DYRK3 Couples Stress Granule Condensation/Dissolution to mTORC1 Signaling

(2013) Frank Wippich et al.   CELL

Alternative splicing of intrinsically disordered regions and rewiring of protein interactions

(2013) Marija Buljan et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Intrinsic Disorder-based Protein Interactions and their Modulators

(2013) Vladimir N. Uversky   CURRENT PHARMACEUTICAL DESIGN

Phase transitions and size scaling of membrane-less organelles

(2013) Clifford P. Brangwynne   JOURNAL OF CELL BIOLOGY

Molecular Crowding Stabilizes Both the Intrinsically Disordered Calcium-Free State and the Folded Calcium-Bound State of a Repeat in Toxin (RTX) Protein

(2013) Ana-Cristina Sotomayor-Pérez et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ligand Concentration Regulates the Pathways of Coupled Protein Folding and Binding

(2013) Kyle G. Daniels et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization

(2013) Timothy Jarvela et al.   MOLECULAR BIOLOGY OF THE CELL

A nuclear F-actin scaffold stabilizes ribonucleoprotein droplets against gravity in large cells

(2013) Marina Feric et al.   NATURE CELL BIOLOGY

A decade and a half of protein intrinsic disorder: Biology still waits for physics

(2013) Vladimir N. Uversky   PROTEIN SCIENCE

Stress granules and cell signaling: more than just a passing phase?

(2013) Nancy Kedersha et al.   TRENDS IN BIOCHEMICAL SCIENCES

Myelin Membrane Assembly Is Driven by a Phase Transition of Myelin Basic Proteins Into a Cohesive Protein Meshwork

(2013) Shweta Aggarwal et al.   PLOS BIOLOGY

Bacterial in-cell NMR of human α-synuclein: a disordered monomer by nature?: Figure 1

(2012) Andres Binolfi et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Unusual biophysics of intrinsically disordered proteins

(2012) Vladimir N. Uversky   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Rapid Perturbation of Free-Energy Landscapes: From In Vitro to In Vivo

(2012) Hannah Gelman et al.   CHEMISTRY-A EUROPEAN JOURNAL

Hydration Dynamics of a Halophilic Protein in Folded and Unfolded States

(2012) Johan Qvist et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Tissue-Specific Splicing of Disordered Segments that Embed Binding Motifs Rewires Protein Interaction Networks

(2012) Marija Buljan et al.   MOLECULAR CELL

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Effects of Molecular Crowding on the Dynamics of Intrinsically Disordered Proteins

(2012) Elio A. Cino et al.   PLoS One

Exploring the binding diversity of intrinsically disordered proteins involved in one-to-many binding

(2012) Wei-Lun Hsu et al.   PROTEIN SCIENCE

Conditional disorder in chaperone action

(2012) James C.A. Bardwell et al.   TRENDS IN BIOCHEMICAL SCIENCES

Intrinsically disordered proteins may escape unwanted interactions via functional misfolding

(2011) Vladimir N. Uversky   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Effects of Macromolecular Crowding on an Intrinsically Disordered Protein Characterized by Small-Angle Neutron Scattering with Contrast Matching

(2011) Daniel Johansen et al.   BIOPHYSICAL JOURNAL

Calcium-induced Folding of Intrinsically Disordered Repeat-in-Toxin (RTX) Motifs via Changes of Protein Charges and Oligomerization States

(2011) Ana Cristina Sotomayor-Pérez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Macromolecular Crowding Fails To Fold a Globular Protein in Cells

(2011) Alexander P. Schlesinger et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Comparative Analysis of Viscosity of Complex Liquids and Cytoplasm of Mammalian Cells at the Nanoscale

(2011) Tomasz Kalwarczyk et al.   NANO LETTERS

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biogenesis and function of nuclear bodies

(2011) Yuntao S. Mao et al.   TRENDS IN GENETICS

Small-angle neutron scattering and contrast variation: a powerful combination for studying biological structures

(2010) William T. Heller   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Coacervation of tropoelastin

(2010) Giselle C. Yeo et al.   ADVANCES IN COLLOID AND INTERFACE SCIENCE

Probing the Folding Intermediate ofBacillus subtilisRNase P Protein by Nuclear Magnetic Resonance

(2010) Yu-Chu Chang et al.   BIOCHEMISTRY

Osmolyte-Induced Folding of an Intrinsically Disordered Protein: Folding Mechanism in the Absence of Ligand

(2010) Yu-Chu Chang et al.   BIOCHEMISTRY

Factors Defining Effects of Macromolecular Crowding on Protein Stability: An in Vitro/in Silico Case Study Using Cytochromec

(2010) Alexander Christiansen et al.   BIOCHEMISTRY

Understanding protein non-folding

(2010) Vladimir N. Uversky et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Multitude of binding modes attainable by intrinsically disordered proteins: a portrait gallery of disorder-based complexes

(2010) Vladimir N. Uversky   CHEMICAL SOCIETY REVIEWS

Cellular structural biology

(2010) Yutaka Ito et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Targeting intrinsically disordered proteins in neurodegenerative and protein dysfunction diseases: another illustration of the D2concept

(2010) Vladimir N Uversky   Expert Review of Proteomics

Intrinsic disorder in proteins associated with neurodegenerative diseases

(2010) Vladimir et al.   Frontiers in Bioscience-Landmark

Protein folding stability and dynamics imaged in a living cell

(2010) Simon Ebbinghaus et al.   NATURE METHODS

The Role of GRASP65 in Golgi Cisternal Stacking and Cell Cycle Progression

(2010) Danming Tang et al.   TRAFFIC

Biogenesis of Nuclear Bodies

(2010) M. Dundr et al.   Cold Spring Harbor Perspectives in Biology

Close encounters of the third kind: disordered domains and the interactions of proteins

(2009) Peter Tompa et al.   BIOESSAYS

Effect of Macromolecular Crowding on Protein Binding Stability: Modest Stabilization and Significant Biological Consequences

(2009) Jyotica Batra et al.   BIOPHYSICAL JOURNAL

Unfoldomics of human diseases: linking protein intrinsic disorder with diseases

(2009) Vladimir N Uversky et al.   BMC GENOMICS

Linking folding and binding

(2009) Peter E Wright et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The Mysterious Unfoldome: Structureless, Underappreciated, Yet Vital Part of Any Given Proteome

(2009) Vladimir N. Uversky   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Unfoldomics of Human Genetic Diseases: Illustrative Examples of Ordered and Intrinsically Disordered Members of the Human Diseasome

(2009) Uros Midic et al.   PROTEIN AND PEPTIDE LETTERS

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Structural Basis for the Aminoacid Composition of Proteins from Halophilic Archea

(2009) Xavier Tadeo et al.   PLOS BIOLOGY

Macromolecular Crowding and Confinement: Biochemical, Biophysical, and Potential Physiological Consequences

(2008) Huan-Xiang Zhou et al.   Annual Review of Biophysics

Intrinsically Disordered Proteins in Human Diseases: Introducing the D2 Concept

(2008) Vladimir N. Uversky et al.   Annual Review of Biophysics

Macromolecular Crowding and Size Effects on Probe Microviscosity

(2008) Aron B. Goins et al.   BIOPHYSICAL JOURNAL

The unfoldomics decade: an update on intrinsically disordered proteins

(2008) A Keith Dunker et al.   BMC GENOMICS

Flexible nets: disorder and induced fit in the associations of p53 and 14-3-3 with their partners

(2008) Christopher J Oldfield et al.   BMC GENOMICS

LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana

(2008) Michaela Hundertmark et al.   BMC GENOMICS

Function and structure of inherently disordered proteins

(2008) A Keith Dunker et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A careful disorderliness in the proteome: Sites for interaction and targets for future therapies

(2008) Robert B. Russell et al.   FEBS LETTERS

Temperature sensitive peptides: Engineering hyperthermia-directed therapeutics

(2008) J. Andrew Mackay et al.   INTERNATIONAL JOURNAL OF HYPERTHERMIA

NMR observation of Tau in Xenopus oocytes

(2008) Jean-François Bodart et al.   JOURNAL OF MAGNETIC RESONANCE

The Antitermin ation Activity of Bacteriophage λ N Protein is Controlled by the Kinetics of an RNA-Looping-Facilitated Interaction with the Transcription Complex

(2008) Clarke R. Conant et al.   JOURNAL OF MOLECULAR BIOLOGY

Signal transduction via unstructured protein conduits

(2008) A Keith Dunker et al.   Nature Chemical Biology

Mimicking the Plant Cell Interior under Water Stress by Macromolecular Crowding: Disordered Dehydrin Proteins Are Highly Resistant to Structural Collapse

(2008) J.-M. Mouillon et al.   PLANT PHYSIOLOGY

The Enigmatic LEA Proteins and Other Hydrophilins

(2008) M. Battaglia et al.   PLANT PHYSIOLOGY

Intrinsic disorder in scaffold proteins: Getting more from less

(2008) Marc S. Cortese et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Effect of mixed macromolecular crowding agents on protein folding

(2008) Huan-Xiang Zhou   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

BIOCHEMISTRY: Controlled Chaos

(2008) V. N. Uversky et al.   SCIENCE

Fuzzy complexes: polymorphism and structural disorder in protein–protein interactions

(2007) Peter Tompa et al.   TRENDS IN BIOCHEMICAL SCIENCES

Solvent-induced collapse of α-synuclein and acid-denatured cytochrome c

(2002) Artemiza S. Morar et al.   PROTEIN SCIENCE