Article
Biochemistry & Molecular Biology
Li Wang, Dianfu Zhang, Sai Li, Long Wang, Jiaojiao Yin, Zhen Xu, Xuezhen Zhang
Summary: Selenium supplementation within the nutritional range promotes the growth of rainbow trout white muscle by inhibiting protein degradation pathways and facilitating myoblast fusion. This study provides insights into the regulatory role of selenium in fish growth.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Cell Biology
Aleksandra Ulman, Marta Kot, Klaudia Skrzypek, Barbara Szewczyk, Marcin Majka
Summary: Induced pluripotent stem cells are a useful tool for studying human embryo development processes such as myogenesis due to their ability to differentiate into three germ layers. Different protocols for obtaining myogenic cells have been described, with Protocol III found to be the most efficient. CD56 was also found to not be a specific marker for evaluating myogenic differentiation.
Article
Oncology
Peggie Chien, Haibin Xi, April D. Pyle
Summary: This review describes the current state of human pluripotent stem cells (hPSCs) in recapitulating human myogenesis ex vivo, and discusses the potential of differentiating hPSCs into muscle stem cells for regenerative medicine, as well as considerations of stem cell and progenitor cell state and function.
EXPERIMENTAL CELL RESEARCH
(2022)
Review
Cell Biology
Hadi Boukhatmi
Summary: Experiments in Drosophila have been essential in understanding the molecular and cellular mechanisms regulating MuSCs, with a rich genetic toolbox available. The study revealed how AMPs in fruit flies are positioned, acquire identity, and are controlled by environmental signals. Recent identification of adult MuSCs in fruit flies with similar characteristics to vertebrates opens up new possibilities for muscle stem cell biology research.
Article
Developmental Biology
Olga G. Jaime, Jessica Arias, Shreya Pavani, April D. Pyle, Michael R. Hicks
Summary: The co-expression of SIX1 and PAX3 is the most indicative factor for skeletal muscle differentiation in human pluripotent stem cells (hPSCs). Manipulating seeding density, monitoring metabolic secretion, and altering the concentration of CHIR99021 can enhance the emergence of SIX1+PAX3+ precursors in hPSC differentiation. Inhibition of non-myogenic lineages can modulate PAX3 expression independently of SIX1.
Article
Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Qiang An, Rui-Men Zhang, Yao Wei, Yong-Wang Zhang, Le-Yi Wang, Shi-Nan Ma, Er-Kang Zhang, Chao-Xia Zou, Su-Fang Yang, De-Shun Shi, Ying-Ming Wei, Yan-Fei Deng
Summary: In this study, a newly identified circular RNA, circRRAS2, was found to be significantly upregulated during the differentiation phase of bovine muscle stem cells (MuSCs). CircRRAS2 was shown to inhibit MuSCs proliferation and promote myoblast differentiation. Furthermore, chromatin isolation with RNA purification and mass spectrometry identified 52 RNA-binding proteins that could potentially interact with circRRAS2 to regulate muscle cell differentiation. These findings suggest that circRRAS2 may serve as a specific regulator of myogenesis in bovine muscle.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Cell Biology
Jan A. Mennigen, Julianne Magnan, Kenan Touma, Carol Best, Brett M. Culbert, Nicholas J. Bernier, Kathleen M. Gilmour
Summary: Juvenile rainbow trout develop social hierarchies in constrained environments, leading to changes in energy metabolism based on social status. The somatotropic axis, which regulates metabolism and growth, may be involved in mediating these metabolic changes. This study found that social status influenced the expression of growth hormone receptor paralogues, leading to differential activation of anabolic and catabolic pathways in subordinate rainbow trout.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
HoTae Lim, In Young Choi, Sang-Hwan Hyun, Hyesoo Kim, Gabsang Lee
Summary: Using hPSCs to generate skeletal muscle tissues in vitro provides a valuable model for understanding molecular functions and gene regulatory networks involved in muscle differentiation and disease pathology. The combination of established myogenesis protocols and molecular profiling techniques offers insights into normal and disease skeletal muscle development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Joe Ibarra, Tyler Hershenhouse, Luay Almassalha, David Walterhouse, Vadim Backman, Kyle L. Macquarrie
Summary: The study found chromosome-specific differences in the organizational characteristics of chromosomes during normal myogenesis, with chromosome 2 showing increased area occupation and a shift in peripheral localization. Additionally, it was discovered that the positioning of chromosome 2 changes during differentiation, with a preference for positioning along the major nuclear axis only in myotubes, whereas RMS cells do not display such preference.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Ruimen Zhang, Jinling Wang, Zhengzhong Xiao, Chaoxia Zou, Qiang An, Hui Li, Xiaoqing Zhou, Zhuyue Wu, Deshun Shi, Yanfei Deng, Sufang Yang, Yingming Wei
Summary: The study revealed a significant number of differentially expressed genes during buffalo MuSCs differentiation, enriching in crucial biological processes such as cell cycle and p53 signaling pathway. Interference with MYLPF expression was found to significantly inhibit MuSCs differentiation.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Yu Fu, Peng Shang, Bo Zhang, Xiaolong Tian, Ruixue Nie, Ran Zhang, Hao Zhang
Summary: The translation above suggests that the TRPC1 gene may promote muscle growth and development through activating Wnt signaling pathways and play a key role in myogenesis.
Article
Biochemistry & Molecular Biology
Manuel Schmidt, Christine Poser, Christina Janster, Julia von Maltzahn
Summary: The hairpin region of WNT7A in its C-terminus is fully sufficient to induce non-canonical signaling in myogenic cells, leading to hypertrophy, expansion of satellite stem cells, and migration of myoblasts. The linker region in the C-terminus of WNT7A binds to the FZD7 receptor but does not activate non-canonical Wnt signaling. The hairpin and linker regions of WNT7A can independently activate canonical Wnt signaling.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Review
Cell Biology
Ryan E. Kahn, Sudarshan Dayanidhi, Orly Lacham-Kaplan, John A. Hawley
Summary: Skeletal muscle, which accounts for approximately 50% of body mass, displays a robust diurnal rhythm under the control of the suprachiasmatic nucleus (SCN) in the hypothalamus. Synchronization of peripheral clocks in muscles with the central clock is crucial for coordinated physiological functions. Satellite cells (SCs), a local stem cell population within muscles, have their own functional molecular clock that may regulate SC-mediated muscle regeneration. Understanding the interplay between SC-specific molecular clocks, myogenic programs, and cell cycle kinetics is important for muscle regeneration and repair.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Nicole Verdile, Rolando Pasquariello, Gloriana Cardinaletti, Emilio Tibaldi, Tiziana A. L. Brevini, Fulvio Gandolfi
Summary: Aquaculture is expanding due to high demand for fish consumption, prompting efforts to find sustainable alternative feeds and understand intestinal morphology and physiology. Research on intestinal stem-cell niches in fish species and the role of telocytes in intestinal homeostasis has potential to improve sustainability and growth performance in trout farming.
Article
Cell Biology
Qiyuan Yang, Xingwei Liang, Xiaofei Sun, Lupei Zhang, Xing Fu, Carl J. Rogers, Anna Berim, Shuming Zhang, Songbo Wang, Bo Wang, Marc Foretz, Benoit Viollet, David R. Gang, Buel D. Rodgers, Mei-Jun Zhu, Min Du
Editorial Material
Cardiac & Cardiovascular Systems
Buel D. Rodgers
CIRCULATION RESEARCH
(2016)
Article
Endocrinology & Metabolism
Naisi Li, Qiyuan Yang, Ryan G. Walker, Thomas B. Thompson, Min Du, Buel D. Rodgers
Article
Cell Biology
Catherine E. Winbanks, Kate T. Murphy, Bianca C. Bernardo, Hongwei Qian, Yingying Liu, Patricio V. Sepulveda, Claudia Beyer, Adam Hagg, Rachel E. Thomson, Justin L. Chen, Kelly L. Walton, Kate L. Loveland, Julie R. McMullen, Buel D. Rodgers, Craig A. Harrison, Gordon S. Lynch, Paul Gregorevic
SCIENCE TRANSLATIONAL MEDICINE
(2016)
Article
Biochemistry & Molecular Biology
Bo Wang, Zhixiu Wang, Jeanene M. de Avila, Mei-Jun Zhu, Faya Zhang, Noe Alberto Gomez, Liang Zhao, Qiyu Tian, Junxing Zhao, Joseph Maricelli, Hui Zhang, Buel D. Rodgers, Min Du
Article
Physiology
Jordan D. Rehwaldt, Buel D. Rodgers, David C. Lin
JOURNAL OF APPLIED PHYSIOLOGY
(2017)
Article
Physiology
Joseph W. Maricelli, Denali R. Kagel, Yemeserach M. Bishaw, O. Lynne Nelson, David C. Lin, Buel D. Rodgers
JOURNAL OF APPLIED PHYSIOLOGY
(2017)
Article
Food Science & Technology
Songbo Wang, Xingwei Liang, Qiyuan Yang, Xing Fu, Meijun Zhu, B. D. Rodgers, Qingyan Jiang, Michael V. Dodson, Min Du
MOLECULAR NUTRITION & FOOD RESEARCH
(2017)
Article
Medicine, General & Internal
Bo Wang, Xing Fu, Xingwei Liang, Zhixiu Wang, Qiyuan Yang, Tiande Zou, Wei Nie, Junxing Zhao, Pengfei Gao, Mei-Jun Zhu, Jeanene M. de Avila, Joseph Maricelli, Buel D. Rodgers, Min Du
Article
Biotechnology & Applied Microbiology
Joseph W. Maricelli, Yemeserach M. Bishaw, Bo Wang, Min Du, Buel D. Rodgers
HUMAN GENE THERAPY
(2018)
Article
Endocrinology & Metabolism
Wioletta Czaja, Yukiko K. Nakamura, Naisi Li, Jennifer A. Eldridge, David M. DeAvila, Thomas B. Thompson, Buel D. Rodgers
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2019)
Article
Cell Biology
Axel J. Fenwick, Peter O. Awinda, Jacob A. Yarbrough-Jones, Jennifer A. Eldridge, Buel D. Rodgers, Bertrand C. W. Tanner
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2019)
Article
Medicine, Research & Experimental
Buel D. Rodgers, Yemeserach Bishaw, Denali Kagel, Julian N. Ramos, Joseph W. Maricelli
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2020)
Review
Endocrinology & Metabolism
Buel D. Rodgers, Christopher W. Ward
Summary: Muscle wasting diseases are debilitating and often deadly, with current treatment strategies relatively ineffective. Promising treatments in development target disrupting myostatin and activin receptor signaling, as well as involve antibodies and gene therapeutics. Success in these developments could revolutionize the treatment of muscle wasting conditions.
Article
Medicine, Research & Experimental
Buel D. Rodgers, Sarah K. Herring, Dereck R. Carias, Joyce Chen, Agostinho G. Rocha
Summary: A fit-for-purpose model process was developed to validate a GLP-compliant ddPCR assay for AVGN7 gene therapy. The method demonstrated high precision, accuracy, and sensitivity, exceeding established standards in the field.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)