Molecular and Clinical Aspects of Protein Aggregation Assays in Neurodegenerative Diseases

Diameter of the vial plays a crucial role in the amyloid fibril formation: Role of interface area between hydrophilic-hydrophobic surfaces

(2017) Jayaraman Jayamani et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

High-Throughput Screening Methodology to Identify Alpha-Synuclein Aggregation Inhibitors

(2017) Jordi Pujols et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Prion seeding activity and infectivity in skin samples from patients with sporadic Creutzfeldt-Jakob disease

(2017) Christina D. Orrú et al.   Science Translational Medicine

High diagnostic value of second generation CSF RT-QuIC across the wide spectrum of CJD prions

(2017) Alessia Franceschini et al.   Scientific Reports

Diagnosis of Human Prion Disease Using Real-Time Quaking-Induced Conversion Testing of Olfactory Mucosa and Cerebrospinal Fluid Samples

(2017) Matilde Bongianni et al.   JAMA Neurology

Protein aggregation into insoluble deposits protects from oxidative stress

(2017) Anita Carija et al.   Redox Biology

Anti-Prion Screening for Acridine, Dextran, and Tannic Acid using Real Time–Quaking Induced Conversion: A Comparison with PrPSc-Infected Cell Screening

(2017) Jae Wook Hyeon et al.   PLoS One

Heterotypic seeding of Tau fibrillization by pre-aggregated Abeta provides potent seeds for prion-like seeding and propagation of Tau-pathology in vivo

(2016) Bruno Vasconcelos et al.   ACTA NEUROPATHOLOGICA

Comparative analysis of cerebrospinal fluid biomarkers in the differential diagnosis of neurodegenerative dementia

(2016) Franc Llorens et al.   Alzheimers & Dementia

Aggregated α-Synuclein Increases SOD1 Oligomerization in a Mouse Model of Amyotrophic Lateral Sclerosis

(2016) Yvonne Koch et al.   AMERICAN JOURNAL OF PATHOLOGY

Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt-jakob disease: An international study

(2016) Lynne I. McGuire et al.   ANNALS OF NEUROLOGY

Proteopathic Strains and the Heterogeneity of Neurodegenerative Diseases

(2016) Lary C. Walker   Annual Review of Genetics

Cross-seeding between Aβ40 and Aβ42 in Alzheimer's disease

(2016) Joyce Tran et al.   FEBS LETTERS

Identification of new molecular alterations in fatal familial insomnia

(2016) Franc Llorens et al.   HUMAN MOLECULAR GENETICS

The real-time quaking-induced conversion assay for detection of human prion disease and study of other protein misfolding diseases

(2016) Matthias Schmitz et al.   Nature Protocols

Advanced tests for early and accurate diagnosis of Creutzfeldt–Jakob disease

(2016) Gianluigi Zanusso et al.   Nature Reviews Neurology

Environmental and genetic factors support the dissociation between α-synuclein aggregation and toxicity

(2016) Anna Villar-Piqué et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detection of prions in blood from patients with variant Creutzfeldt-Jakob disease

(2016) L. Concha-Marambio et al.   Science Translational Medicine

Repositioning tolcapone as a potent inhibitor of transthyretin amyloidogenesis and associated cellular toxicity

(2016) Ricardo Sant'Anna et al.   Nature Communications

Progress in CSF biomarker discovery in sCJD

(2016) Franc Llorens et al.   Oncotarget

Detection and Quantification of CWD Prions in Fixed Paraffin Embedded Tissues by Real-Time Quaking-Induced Conversion

(2016) Clare E. Hoover et al.   Scientific Reports

Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

(2016) Graham Fairfoul et al.   Annals of Clinical and Translational Neurology

Application of an in vitro-amplification assay as a novel pre-screening test for compounds inhibiting the aggregation of prion protein scrapie

(2016) Matthias Schmitz et al.   Scientific Reports

Neurofilaments in blood and CSF for diagnosis and prediction of onset in Creutzfeldt-Jakob disease

(2016) Petra Steinacker et al.   Scientific Reports

In Vivo Seeding and Cross-Seeding of Localized Amyloidosis

(2015) Marie E. Oskarsson et al.   AMERICAN JOURNAL OF PATHOLOGY

Insights into the consequences of co-polymerisation in the early stages of IAPP and Aβ peptide assembly from mass spectrometry

(2015) Lydia M. Young et al.   ANALYST

Pathological Interface Between Oligomeric Alpha-Synuclein and Tau in Synucleinopathies

(2015) Urmi Sengupta et al.   BIOLOGICAL PSYCHIATRY

β-Sheet interfering molecules acting against β-amyloid aggregation and fibrillogenesis

(2015) Antonio Francioso et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Inhibition of protein aggregation and amyloid formation by small molecules

(2015) Andrew J Doig et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Effect of acidic and basic pH on Thioflavin T absorbance and fluorescence

(2015) Ellen V. Hackl et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Stability and Reproducibility Underscore Utility of RT-QuIC for Diagnosis of Creutzfeldt-Jakob Disease

(2015) Maria Cramm et al.   MOLECULAR NEUROBIOLOGY

Validation of 14-3-3 Protein as a Marker in Sporadic Creutzfeldt-Jakob Disease Diagnostic

(2015) Matthias Schmitz et al.   MOLECULAR NEUROBIOLOGY

Intracerebral injection of preformed synthetic tau fibrils initiates widespread tauopathy and neuronal loss in the brains of tau transgenic mice

(2015) Eve Peeraer et al.   NEUROBIOLOGY OF DISEASE

Rapid and Sensitive RT-QuIC Detection of Human Creutzfeldt-Jakob Disease Using Cerebrospinal Fluid

(2015) Christina D. Orrú et al.   mBio

A Structural and Functional Comparison Between Infectious and Non-Infectious Autocatalytic Recombinant PrP Conformers

(2015) Geoffrey P. Noble et al.   PLoS Pathogens

Bank Vole Prion Protein As an Apparently Universal Substrate for RT-QuIC-Based Detection and Discrimination of Prion Strains

(2015) Christina D. Orrú et al.   PLoS Pathogens

Amplification of Tau Fibrils from Minute Quantities of Seeds

(2014) Virginia Meyer et al.   BIOCHEMISTRY

Exogenous amyloidogenic proteins function as seeds in amyloid β-protein aggregation

(2014) Kenjiro Ono et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

Anti-amyloid Compounds Inhibit α-Synuclein Aggregation Induced by Protein Misfolding Cyclic Amplification (PMCA)

(2014) Maria Eugenia Herva et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Conformation Determines the Seeding Potencies of Native and Recombinant Tau Aggregates

(2014) Benjamin Falcon et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Conformational Properties of Prion Strains Can Be Transmitted to Recombinant Prion Protein Fibrils in Real-Time Quaking-Induced Conversion

(2014) K. Sano et al.   JOURNAL OF VIROLOGY

Characteristic CSF Prion Seeding Efficiency in Humans with Prion Diseases

(2014) Maria Cramm et al.   MOLECULAR NEUROBIOLOGY

Prions in the Urine of Patients with Variant Creutzfeldt–Jakob Disease

(2014) Fabio Moda et al.   NEW ENGLAND JOURNAL OF MEDICINE

Elements Modulating the Prion Species Barrier and Its Passage Consequences

(2014) Juan-Maria Torres et al.   PLoS One

In vitro aggregation assays for the characterization of α-synuclein prion-like properties

(2014) Joanna Narkiewicz et al.   Prion

Emerging novel concept of chaperone therapies for protein misfolding diseases

(2014) Yoshiyuki SUZUKI   PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES

Prevalence of mixed pathologies in the aging brain

(2014) Jasmin Rahimi et al.   Alzheimers Research & Therapy

Detection of Misfolded Aβ Oligomers for Sensitive Biochemical Diagnosis of Alzheimer’s Disease

(2014) Natalia Salvadores et al.   Cell Reports

The prion protein protease sensitivity, stability and seeding activity in variably protease sensitive prionopathy brain tissue suggests molecular overlaps with sporadic Creutzfeldt-Jakob disease

(2014) Alexander H Peden et al.   Acta Neuropathologica Communications

High-Throughput Biophysical Analysis of Protein Therapeutics to Examine Interrelationships Between Aggregate Formation and Conformational Stability

(2013) Rajoshi Chaudhuri et al.   AAPS Journal

Protein amyloids develop an intrinsic fluorescence signature during aggregation

(2013) Fiona T. S. Chan et al.   ANALYST

Lewy body extracts from Parkinson disease brains trigger α-synuclein pathology and neurodegeneration in mice and monkeys

(2013) Ariadna Recasens et al.   ANNALS OF NEUROLOGY

Design, synthesis and biological activity of multifunctional α,β-unsaturated carbonyl scaffolds for Alzheimer’s disease

(2013) Seema Bag et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Prion-like spreading of pathological α-synuclein in brain

(2013) Masami Masuda-Suzukake et al.   BRAIN

Proteomic data from human cell cultures refine mechanisms of chaperone-mediated protein homeostasis

(2013) Andrija Finka et al.   CELL STRESS & CHAPERONES

A Label-Free, Quantitative Assay of Amyloid Fibril Growth Based on Intrinsic Fluorescence

(2013) Dorothea Pinotsi et al.   CHEMBIOCHEM

Applying chaperones to protein-misfolding disorders: Molecular chaperones against α-synuclein in Parkinson's disease

(2013) Ali Chaari et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Infrared Microspectroscopy Detects Protein Misfolding Cyclic Amplification (PMCA)-induced Conformational Alterations in Hamster Scrapie Progeny Seeds

(2013) Martin L. Daus et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Early Detection of Abnormal Prion Protein in Genetic Human Prion Diseases Now Possible Using Real-Time QUIC Assay

(2013) Kazunori Sano et al.   PLoS One

Assessing the causes and consequences of co-polymerization in amyloid formation

(2013) Claire J Sarell et al.   Prion

Recombinant Human Prion Protein Inhibits Prion Propagation in vitro

(2013) Jue Yuan et al.   Scientific Reports

The Evolution of Transmissible Prions: The Role of Deformed Templating

(2013) Natallia Makarava et al.   PLoS Pathogens

Cross-Seeding of Misfolded Proteins: Implications for Etiology and Pathogenesis of Protein Misfolding Diseases

(2013) Rodrigo Morales et al.   PLoS Pathogens

Establishing quantitative real-time quaking-induced conversion (qRT-QuIC) for highly sensitive detection and quantification of PrPSc in prion-infected tissues

(2013) Song Shi et al.   Acta Neuropathologica Communications

Dye-Binding Assays for Evaluation of the Effects of Small Molecule Inhibitors on Amyloid (Aβ) Self-Assembly

(2012) Laramie P. Jameson et al.   ACS Chemical Neuroscience

Real time quaking-induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt-Jakob disease

(2012) Lynne I. McGuire et al.   ANNALS OF NEUROLOGY

Cross-Seeding of Fibrils from Two Types of Insulin Induces New Amyloid Strains

(2012) Weronika Surmacz-Chwedoruk et al.   BIOCHEMISTRY

The Amyloid State of Proteins in Human Diseases

(2012) David Eisenberg et al.   CELL

Protein aggregation: Mechanisms and functional consequences

(2012) Gaetano Invernizzi et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Discovery of Novel Inhibitors of Amyloid β-Peptide 1–42 Aggregation

(2012) Laura C. López et al.   JOURNAL OF MEDICINAL CHEMISTRY

Cross-seeding effects of amyloid β-protein and α-synuclein

(2012) Kenjiro Ono et al.   JOURNAL OF NEUROCHEMISTRY

Using bacterial inclusion bodies to screen for amyloid aggregation inhibitors

(2012) Anna Villar-Piqué et al.   Microbial Cell Factories

PaxDb, a Database of Protein Abundance Averages Across All Three Domains of Life

(2012) M. Wang et al.   MOLECULAR & CELLULAR PROTEOMICS

New generation QuIC assays for prion seeding activity

(2012) Christina D. Orru et al.   Prion

Distinguishing crystal-like amyloid fibrils and glass-like amorphous aggregates from their kinetics of formation

(2012) Y. Yoshimura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Japanese Mutant Aβ (ΔE22-Aβ1−39) Forms Fibrils Instantaneously, with Low-Thioflavin T Fluorescence: Seeding of Wild-Type Aβ1−40into Atypical Fibrils by ΔE22-Aβ1−39

(2011) Adam L. Cloe et al.   BIOCHEMISTRY

Mechanism of amyloidogenesis: nucleation-dependent fibrillation versus double-concerted fibrillation

(2011) Ghi-Bom Bhak et al.   BMB Reports

Role of metal ions in aggregation of intrinsically disordered proteins in neurodegenerative diseases

(2011) Leonid Breydo et al.   Metallomics

Molecular chaperones in protein folding and proteostasis

(2011) F. Ulrich Hartl et al.   NATURE

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion

(2011) Ryuichiro Atarashi et al.   NATURE MEDICINE

In vivo demonstration that  -synuclein oligomers are toxic

(2011) B. Winner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accelerated High Fidelity Prion Amplification Within and Across Prion Species Barriers

(2011) Kristi M. Green et al.   PLoS Pathogens

Ultra-Efficient PrPSc Amplification Highlights Potentialities and Pitfalls of PMCA Technology

(2011) Gian Mario Cosseddu et al.   PLoS Pathogens

Metal Emissions and Urban Incident Parkinson Disease: A Community Health Study of Medicare Beneficiaries by Using Geographic Information Systems

(2010) A. W. Willis et al.   AMERICAN JOURNAL OF EPIDEMIOLOGY

Strategies to increase the reproducibility of protein fibrillization in plate reader assays

(2010) Lise Giehm et al.   ANALYTICAL BIOCHEMISTRY

Molecular mechanism of Thioflavin-T binding to amyloid fibrils

(2010) Matthew Biancalana et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Early detection of amyloid aggregation using intrinsic fluorescence

(2010) Olaf J. Rolinski et al.   BIOSENSORS & BIOELECTRONICS

Mammalian Prions Generated from Bacterially Expressed Prion Protein in the Absence of Any Mammalian Cofactors

(2010) Jae-Il Kim et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Role of Protein Sequence and Amino Acid Composition in Amyloid Formation: Scrambling and Backward Reading of IAPP Amyloid Fibrils

(2010) Raimon Sabaté et al.   JOURNAL OF MOLECULAR BIOLOGY

Coinfecting Prion Strains Compete for a Limiting Cellular Resource

(2010) R. A. Shikiya et al.   JOURNAL OF VIROLOGY

Pharmacological chaperone for the structured domain of human prion protein

(2010) Andrew J. Nicoll et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays

(2010) Jason M. Wilham et al.   PLoS Pathogens

Updated clinical diagnostic criteria for sporadic Creutzfeldt-Jakob disease

(2009) I. Zerr et al.   BRAIN

The role of glycophosphatidylinositol anchor in the amplification of the scrapie isoform of prion protein in vitro

(2009) Jae-Il Kim et al.   FEBS LETTERS

Crowded Cell-like Environment Accelerates the Nucleation Step of Amyloidogenic Protein Misfolding

(2009) Zheng Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Interactions of thioflavin T with serum albumins: Spectroscopic analyses

(2009) Priyankar Sen et al.   SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

A novel human disease with abnormal prion protein sensitive to protease

(2008) Pierluigi Gambetti et al.   ANNALS OF NEUROLOGY

Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1

(2008) Jung-Ho Lee et al.   BIOCHEMICAL JOURNAL

A comparative study of microbubble generation by mechanical agitation and sonication

(2008) Qingyi Xu et al.   Innovative Food Science & Emerging Technologies

Concomitant TAR-DNA-Binding Protein 43 Pathology Is Present in Alzheimer Disease and Corticobasal Degeneration but Not in Other Tauopathies

(2008) Kunihiro Uryu et al.   JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY

Protein aggregation: Pathways, induction factors and analysis

(2008) Hanns-Christian Mahler et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

On the binding of Thioflavin-T to HET-s amyloid fibrils assembled at pH 2

(2008) Raimon Sabaté et al.   JOURNAL OF STRUCTURAL BIOLOGY

Coexistence of Creutzfeldt-Jakob disease, Lewy body disease, and Alzheimer's disease pathology: An autopsy case showing typical clinical features of Creutzfeldt-Jakob disease

(2008) Takashi Haraguchi et al.   NEUROPATHOLOGY

Extrinsic Fluorescent Dyes as Tools for Protein Characterization

(2008) Andrea Hawe et al.   PHARMACEUTICAL RESEARCH

The Efficacy of Tetracyclines in Peripheral and Intracerebral Prion Infection

(2008) Ada De Luigi et al.   PLoS One

Mechanisms of prion protein assembly into amyloid

(2008) J. Stohr et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Folding versus aggregation: Polypeptide conformations on competing pathways

(2007) Thomas R. Jahn et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS