Article
Multidisciplinary Sciences
Janneke Elzinga, Benthe van der Lugt, Clara Belzer, Wilma T. Steegenga
Summary: HT29-MTX-E12 cells grown under SWMS conditions showed increased MUC2 production, stimulated cell growth, and exhibited a more aerobic cell metabolism. This model may have potential applications in future studies.
Article
Biochemistry & Molecular Biology
Xiaowan Feng, Hui Zhang, Phoency F. H. Lai, Zhiqiang Xiong, Lianzhong Ai
Summary: A pyruvated exopolysaccharide named LPE-1 was isolated and characterized from the fermentation broth of Lactobacillus plantarum AR307. LPE-1 was found to contain Galp and Glcp at a molar ratio of 2:1, with a molecular weight of 605 kDa. The presence of pyruvate group in LPE-1 plays a significant role in improving the viscosity and plasticity of dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Mohsen Zommara, Shady El-Ghaish, Thomas Haertle, Jean-Marc Chobert, Mohamed Ghanimah
Summary: In this study, Lactobacillus strains were isolated from conventional Egyptian cheeses. Among them, L. paracasei BD3, L. plantarum BR4, and L. fermentum MR2 showed potential probiotic and technological characteristics, which can be applied in cheese making. This research is significant for improving the quality of cheese using Lactobacillus strains from traditional Egyptian cheeses.
Article
Food Science & Technology
Anas A. Al-Nabulsi, Ziad W. Jaradat, Farah R. Al Qudsi, Lina Elsalem, Tareq M. Osaili, Amin N. Olaimat, Gennaro Esposito, Shao-Quan Liu, Mutamed M. Ayyash
Summary: This study investigates and characterizes the bioactivities of two novel exopolysaccharides, EPS-S and EPS-L, and reveals their potential applications in the food and nutraceutical industries, as they exhibit anticancer, antimicrobial, and enzyme inhibitory activities.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Shu-Min Hu, Jia-Min Zhou, Qing-Qing Zhou, Ping Li, Yuan-Yuan Xie, Tao Zhou, Qing Gu
Summary: Exopolysaccharides (EPS) produced by Lactobacillus rhamnosus ZFM231 isolated from milk were purified and separated into four fractions, showing antioxidant and hypolipidemic activities, with potential applications in the food and pharmaceutical industries.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Nutrition & Dietetics
Jiayuan Zhao, Lihan Wang, Shasha Cheng, Yu Zhang, Mo Yang, Ruxue Fang, Hongxuan Li, Chaoxin Man, Yujun Jiang
Summary: The study found that exopolysaccharides (EPS) from Lactobacillus plantarum JY039 can improve the adhesion ability of Lactobacillus paracasei JY062 to cells and have the potential to prevent and alleviate type 2 diabetes (T2D). The EPS and L. paracasei JY062 can also alter the structure of the intestinal microbiota, enhance intestinal barrier function, promote gut hormone secretion, and reduce inflammation.
Article
Food Science & Technology
Jingfei Hu, Xueqing Tian, Tong Wei, Hangjie Wu, Jing Lu, Mingsheng Lyu, Shujun Wang
Summary: The exopolysaccharide (EPS) extracted from Lactobacillus plajomi PW-7 showed antibacterial activity and antioxidant capacity against Helicobacter pylori. It has a potential application in developing EPS-based foods. The structural characteristics and biological activity of EPS play a key role in its potential applications.
Article
Food Science & Technology
Carlotta Giromini, Antonella Baldi, Raffaella Rebucci, Davide Lanzoni, Martina Policardi, Tamil Selvi Sundaram, Stig Purup
Summary: This study established an in vitro stress model of inflammatory bowel disease using HT29-MTX-E12 cells treated with DSS, and investigated the protective role of short chain fatty acids on intestinal barrier integrity under stress conditions. The results showed that specific concentrations of butyrate and SCFAs-MIX can alleviate DSS-induced epithelial cell damage and promote the expression of related genes.
Article
Biochemistry & Molecular Biology
Nisha Sharma, Shaheen Wasil Kabeer, Inder Pal Singh, Kulbhushan Tikoo
Summary: This study investigated the conjugation of cisplatin with an exopolysaccharide from Lactobacillus gasseri (LG-EPS) and found that the resulting complex showed enhanced and selective anticancer activity against tumor cells overexpressing GLUT1. The complex had increased solubility and better pharmacokinetic profile compared to cisplatin, and its cytotoxicity correlated with GLUT1 expression. The study suggests that this green glycoconjugation can be a safe and effective strategy to broaden the therapeutic potential of cisplatin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Min Zhang, Tiantian Lai, Mengke Yao, Man Zhang, Zhennai Yang
Summary: The study found interactions between EPS produced by LAB and casein, resulting in improved physical properties and bioactivities of their complex. These interaction mechanisms are important for enhancing the functionality of fermented dairy products.
Article
Environmental Sciences
Oceane Reale, Antoine Huguet, Valerie Fessard
Summary: This study evaluated the toxic effects of four lipophilic phycotoxins on Caco-2/HT29-MTX co-cultured cells, revealing that these toxins can reduce cell viability, affect monolayer integrity, and have different impacts on cell types and mucus production. Cell models integrating multiple cell types may improve hazard characterization and provide more accurate descriptions of the modes of action of phycotoxins.
Article
Pharmacology & Pharmacy
Yi Cheng, Chie Watanabe, Yusuke Ando, Satoshi Kitaoka, Yuya Egawa, Tomoya Takashima, Akihiro Matsumoto, Masahiro Murakami
Summary: In this study, researchers developed a partially laminated cell culture model to simulate the mucus layer in the human intestines. Compared to traditional coculture models, this partially laminated model maintained a higher barrier function and adjustable permeability according to the cell ratio. This novel model may have potential applications in vitro for evaluating intestinal mucosal permeability and drug efficacy.
Article
Food Science & Technology
Yuzhu Zhu, Jia-Min Zhou, Wei Liu, Xionge Pi, Qingqing Zhou, Ping Li, Tao Zhou, Qing Gu
Summary: The study identified four types of EPS with different molecular composition from Lactobacillus rhamnosus ZFM231, and evaluated their potential impact on the human gut microbiota through in vitro fermentation model. Results showed that EPS could be effectively degraded by human gut microbiota and fermented to produce short chain fatty acids. Furthermore, EPS mainly increased the abundance of beneficial bacteria such as Ruminococcus, Dorea, Butyricicoccus, and Blautia. This research provides valuable insights into the mechanisms of Lactobacillus rhamnosus EPS in the gastrointestinal tract of the human body.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Yusuke Ueda, Haruta Mogami, Yosuke Kawamura, Masahito Takakura, Asako Inohaya, Eriko Yasuda, Yu Matsuzaka, Yoshitsugu Chigusa, Shinji Ito, Masaki Mandai, Eiji Kondoh
Summary: Cervical excision leads to decreased expression of MUC5B and MUC5AC in cervicovaginal fluid, which may affect cervical function in pregnant women. The study suggests that MUC5B and MUC5AC may serve as barriers against ascending pathogens during pregnancy. This finding has important implications for understanding the role of the cervix in maintaining pregnancy.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Agriculture, Dairy & Animal Science
Yu Zhang, Xiaofei Dai, Haonan Jin, Chaoxin Man, Yujun Jiang
Summary: This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, which showed that optimizing carbon source not only increases EPS production, but also alters EPS monosaccharide composition by enhancing enzyme activity in the synthesis pathways.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Teresa D. Rebaza-Cardenas, Kenneth Silva-Cajaleon, Carlos Sabater, Susana Delgado, Nilda D. Montes-Villanueva, Patricia Ruas-Madiedo
Summary: In this study, lactic acid bacteria were isolated and identified from two traditional Peruvian beverages. The strain Lac. plantarum Ch13 showed potential as a probiotic candidate with better resistance to gastrointestinal conditions and higher adhesion ability compared to a commercial probiotic strain.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Review
Biotechnology & Applied Microbiology
Felipe Gonzalez-Gonzalez, Susana Delgado, Lorena Ruiz, Abelardo Margolles, Patricia Ruas-Madiedo
Summary: Fermentation has traditionally been used for food preservation, but modern industrial processes aim to improve the functional characteristics of the final product. Starter cultures, adjunct cultures, and probiotics play essential roles in achieving high-quality foods with improved nutritional and health-promoting properties. This review focuses on the functional characteristics of these cultures, including their synthesis of metabolites for preservation and safety, their impact on organoleptic properties, and their contributions to nutrition and health improvement.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Brankica Filipic, Milka Malesevic, Zorica Vasiljevic, Katarina Novovic, Milan Kojic, Branko Jovcic
Summary: This study found that 33 out of 98 Achromobacter spp. isolates from pediatric patients were resistant to SXT, and among these, 10 resistant isolates were found to carry the intI1 gene. Genome sequencing revealed a deletion of 86 amino acids in the IntI1S protein compared to canonical IntI1 protein. The results suggest that IntI1S isolates are derived from clinical settings and that cystic fibrosis patients may serve as potential reservoirs for healthcare-associated infections in non-cystic fibrosis patients.
FOLIA MICROBIOLOGICA
(2023)
Article
Virology
Mina Obradovic, Milka Malesevic, Mariagrazia Di Luca, Dusan Kekic, Ina Gajic, Olivia McAuliffe, Horst Neve, Nemanja Stanisavljevic, Goran Vukotic, Milan Kojic
Summary: Klebsiella pneumoniae is a global health threat and phages LASTA and SJM3 show potential in combating pandrug-resistant K. pneumoniae infections. The two phages have narrow host ranges and long latent periods, but are not lysogenic. Genome analysis reveals they belong to the new genus Lastavirus. In tests, both phages demonstrate significant bacterial reduction, with SJM3 performing slightly better.
Article
Microbiology
Katarina Novovic, Snezana Kuzmanovic Nedeljkovic, Mirjana Poledica, Gordana Nikolic, Bojana Grujic, Branko Jovcic, Milan Kojic, Brankica Filipic
Summary: Since the declaration of the COVID-19 pandemic, the disease has overwhelmed the health system, leading to an increase in ICU admissions. This study examines the antibiotic resistance and virulence potential of A. baumannii isolates recovered from COVID-19 patients requiring mechanical ventilation. The results reveal the presence of multidrug-resistant A. baumannii strains with varying levels of antibiotic resistance and virulence potential.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Gastroenterology & Hepatology
Anna Samarra, Maria Esteban-Torres, Raul Cabrera-Rubio, Manuel Bernabeu, Silvia Arboleya, Miguel Gueimonde, Maria Carmen Collado
Summary: Resistance to antibiotics is a global threat, especially for newborns whose immune systems are still developing. The moment of birth exposes infants to antibiotic-resistant microorganisms that can colonize their gut and other body sites. Understanding the vertical transmission of antibiotic resistance from mothers to their offspring is crucial for developing strategies to reduce antibiotic resistance in early life.
Article
Microbiology
Felix Royo, Hector Tames, Guillermo Bordanaba-Florit, Diana Cabrera, Maria Azparren-Angulo, Clara Garcia-Vallicrosa, Abelardo Margolles, Lorena Ruiz, Patricia Ruas-Madiedo, Juan M. Falcon-Perez
Summary: This study used mice as a model and fed them with a bacterial strain of B. adolescentis that can degrade glutamate and convert it into GABA. The bacterium can survive in the gastric tract and the animals reduce their blood glutamate concentration over time. The results suggest that an oral diet with this probiotic-type bacteria could reduce the concentration of glutamate in the blood, providing a reference for clinical trial studies in patients with chronic diseases.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Microbiology
Gonzalo Solis, Miguel Gueimonde
Article
Biochemistry & Molecular Biology
Marinela Sokarda Slavic, Milan Kojic, Aleksandra Margetic, Nemanja Stanisavljevic, Lazar Gardijan, Natasa Bozic, Zoran Vujcic
Summary: Amylase from thermophilic bacterial strain Anoxybacillus vranjensis ST4 was cloned, expressed, and purified successfully. The enzyme showed maximum activity at 60-80°C and was active and stable over a wide pH range (4.0-9.0). It efficiently hydrolyzed starch granules, releasing maltose, maltotriose, and maltopentaose as the main products. These findings indicate that this highly stable and versatile enzyme has great potential for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Marinela Sokarda Slavic, Milan Kojic, Aleksandra Margetic, Marina Ristovic, Marija Pavlovic, Stefan Nikolic, Zoran Vujcic
Summary: The study focused on the impact of the enzyme endo-beta-1,3-1,4-glucanase from Bacillus subtilis 168 on improving the nutritional and bioactive properties of barley beta-glucan. A new procedure for isolating pure beta-glucan from barley flour was developed, enabling it to be free from starch contamination. The cloned enzyme showed high efficiency in degrading beta-glucan and increasing its antioxidant capacities, suggesting potential applications in functional foods.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Aida Zapico, Silvia Arboleya, Nuria Salazar, Carmen Perillan, Sergio Ruiz-Saavedra, Clara G. de los Reyes-Gavilan, Miguel Gueimonde, Sonia Gonzalez
Summary: Non-communicable diseases are prevalent among low-income individuals due to the consumption of processed foods, fat, and sugars. This study evaluated the effects of a nutrition education intervention on sensory perception, health-related parameters, and gut microbiota of low socio-economic individuals. The intervention led to positive changes in dietary intake, reduced consumption of potentially carcinogenic compounds, and improved discrimination and depressive symptoms.
Review
Microbiology
Silvia Saturio, Alejandra Rey, Anna Samarra, Maria Carmen Collado, Marta Suarez, Laura Mantecon, Gonzalo Solis, Miguel Gueimonde, Silvia Arboleya
Summary: The development of the neonate's intestinal microbiome begins at birth when the mother provides the initial microbial inoculum. This microbiome contains antibiotic resistance genes, which are affected by different perinatal factors. Antibiotics, commonly used in pediatrics, can disrupt the early microbiota and increase the levels of antibiotic resistance genes. Understanding the factors influencing the development of the resistome in early life is crucial due to the emergence of antibiotic-resistant microorganisms.
Article
Microbiology
Alicja M. Nogacka, Isabel Cuesta, Miguel Gueimonde, Clara G. de los Reyes-Gavilan
Summary: Breastfeeding is crucial for infant nutrition, and 2-fucosyllactose (2'FL) is an important bioactive component in breastmilk. This study found a synergistic effect between Bifidobacterium bifidum IPLA20048 and Lactobacillus gasseri IPLA20136, where L. gasseri can utilize galactose released from the hydrolysis of 2'FL by B. bifidum. This phenomenon may be common among lactobacilli and bifidobacteria in the infant gut.
Article
Biotechnology & Applied Microbiology
Milka Malesevic, Lazar Gardijan, Marija Miljkovic, Paula M. O'Connor, Nemanja Mirkovic, Branko Jovcic, Paul D. Cotter, Goran Jovanovic, Milan Kojic
Summary: The lactic acid bacterium Lactococcus lactis BGBU1-4 produces a bacteriocin called lactolisterin BU (LBU) which has potent antibacterial activity against Gram-positive pathogens. Additionally, it produces an unknown antibacterial product. It was discovered that LBU breaks down to form a truncated version consisting of 31 amino acids, called LBU1-31, which has 12.5% the antibacterial activity of the full-length LBU. The study also revealed that the N-terminal 24 amino acids of LBU are responsible for antibacterial activity.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sergio Ruiz-Saavedra, Carmen Gonzalez del Rey, Adolfo Suarez, Ylenia Diaz, Aida Zapico, Silvia Arboleya, Nuria Salazar, Miguel Gueimonde, Clara G. de los Reyes-Gavilan, Sonia Gonzalez
Summary: Diet is an important factor that affects the gut microbiota, which in turn plays a role in colorectal cancer development. This study aimed to explore the associations between dietary factors and gut microbiota in different clinical diagnosis groups. The results showed that the intake of red meat and polycyclic aromatic hydrocarbons (PAHs) was associated with changes in the abundance of certain microbial groups. The intake of heterocyclic amines, nitroso compounds, and ethanol was also associated with changes in the abundance of other microbial groups.
Article
Food Science & Technology
Yonghong Ye, Songyan Zheng, Yuanxing Wang
Summary: In this study, the changes of aroma components in Gannan navel orange during growth were systematically studied using HS-SPME-GC-MS. Key aroma components and markers were identified. These findings contribute to a better understanding of the dynamic variation of aroma compounds during navel orange growth and have potential for industrial applications.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Jia Xu, Yayuan Zhang, Mengke Zhang, Xinlin Wei, Yiming Zhou
Summary: This study evaluated the effects of glucosamine selenium on summer-autumn tea, showing that it can enhance nutritional quality, improve sensory characteristics, and increase plant adaptation to environmental changes and abiotic stresses.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Yuanming Chu, Zhaoyang Ding, Jing Xie
Summary: This study investigated the use of ice glazing containing D-sodium erythorbate (DSE) and vacuum packaging to maintain the quality of large yellow croaker during frozen storage. The results showed that vacuum packaging effectively inhibited ice crystal growth and minimized water loss, while the combination of vacuum packaging and 0.3% DSE-infused ice glazing maintained freshness indicators at low levels throughout the 300-day storage period. This combination was considered the most effective in preserving the quality of the fish.
FOOD RESEARCH INTERNATIONAL
(2024)
Review
Food Science & Technology
Md. Ashikur Rahman, Shirin Akter, Md. Ashrafudoulla, Md. Anamul Hasan Chowdhury, A. G. M. Sofi Uddin Mahamud, Si Hong Park, Sang-Do Ha
Summary: This comprehensive review aims to provide insights into the mechanisms and key factors influencing biofilm formation by A. hydrophila in the food industry. It explores the molecular processes involved in various stages of biofilm formation and investigates the impact of intrinsic factors and environmental conditions on biofilm architecture and resilience. The article also highlights the potential of bibliometric analysis in conceptualizing the research landscape and identifying knowledge gaps in A. hydrophila biofilm research.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Chujing Wang, Wenni Tian, Zengliu Song, Qun Wang, Yong Cao, Jie Xiao
Summary: Solid lipid ratio in emulsions has significant effects on colloidal stability, mucus permeability, and bioavailability in vivo. Higher solid lipid ratio improves intestinal stability but reduces mucus permeability.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Dacai Zhong, Liping Kang, Juan Liu, Xiang Li, Li Zhou, Luqi Huang, Zidong Qiu
Summary: In this study, a novel online extraction electrospray ionization mass spectrometry method was developed to comprehensively characterize complex foods. Meanwhile, a characteristic marker screening method and chemometrics modeling were used for the accurate authentication of highly-similar foods. This research has significant implications for ensuring food quality and safety.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Qiannan Zhao, Jinyi Yang, Jiahui Li, Lei Zhang, Xiaohai Yan, Tianli Yue, Yahong Yuan
Summary: This study investigated the transport and hypoglycemic effects of phenolics in mulberry leaves using an in vitro digestion model. The results showed that digested phenolics had higher absorbability and could inhibit glucose digestion and absorption. Phenolics also regulated glucose metabolism. Luteoforol and p-coumaric acid were found to be the primary phenolics strongly correlated with the hypoglycemic ability of mulberry leaves.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Yanyun Cao, Qingling Wang, Jinou Lin, Yin-Yi Ding, Jianzhong Han
Summary: This study investigated the effects of gallic acid (GA) and epigallocatechin gallate (EGCG) at different ratios on the gel properties of calcium induced-whey protein emulsion gel. It was found that GA and EGCG could promote gel formation, increase gel strength, and delay the release of emulsified oil droplets.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Monique Martins Strieder, Vitor Lacerda Sanches, Mauricio Ariel Rostagno
Summary: This study proposed an integrated and automated procedure for extracting, separating, and quantifying bioactive compounds from coffee co-products. By optimizing the extraction, separation, and analysis method, real-time analysis was achieved. The results suggest that coupling different techniques can efficiently extract, separate, and analyze phenolic compounds, providing an integrated method to produce high-added value ingredients for various applications.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Kyu Sang Sim, Hyoyoung Kim, Suel Hye Hur, Tae Woong Na, Ji Hye Lee, Ho Jin Kim
Summary: Geographical origin plays a crucial role in determining the quality and safety of agricultural products. In this study, ICP analysis was used to determine the inorganic elemental content of onions and identify their geographical origin. Chemometric methods were applied to analyze the ICP results, and the accuracy of distinguishing between Korean and Chinese onions was found to be excellent. The findings suggest that this method can be beneficial for identifying agricultural products.
FOOD RESEARCH INTERNATIONAL
(2024)