Article
Biochemistry & Molecular Biology
Xiaowan Feng, Hui Zhang, Phoency F. H. Lai, Zhiqiang Xiong, Lianzhong Ai
Summary: A pyruvated exopolysaccharide named LPE-1 was isolated and characterized from the fermentation broth of Lactobacillus plantarum AR307. LPE-1 was found to contain Galp and Glcp at a molar ratio of 2:1, with a molecular weight of 605 kDa. The presence of pyruvate group in LPE-1 plays a significant role in improving the viscosity and plasticity of dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Jinyu Zhu, Zhongfang Tan, Zhong Zhang, Xianming Shi
Summary: This study investigated the chemical structure, physicochemical properties, antioxidant capacity, antibacterial ability, and anti-biofilm formation activity of an exopolysaccharide (EPS) produced by Lactobacillus curvatus SJTUF 62116. The results showed that EPS-1 is mainly composed of glucose and mannose, and exhibits strong antioxidant and antibacterial properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Nutrition & Dietetics
Jiayuan Zhao, Lihan Wang, Shasha Cheng, Yu Zhang, Mo Yang, Ruxue Fang, Hongxuan Li, Chaoxin Man, Yujun Jiang
Summary: The study found that exopolysaccharides (EPS) from Lactobacillus plantarum JY039 can improve the adhesion ability of Lactobacillus paracasei JY062 to cells and have the potential to prevent and alleviate type 2 diabetes (T2D). The EPS and L. paracasei JY062 can also alter the structure of the intestinal microbiota, enhance intestinal barrier function, promote gut hormone secretion, and reduce inflammation.
Article
Pharmacology & Pharmacy
Renato Kovacs, Lorand Erdelyi, Ferenc Fenyvesi, Noemi Balla, Fruzsina Kovacs, Gyoergy Vamosi, Agnes Klusoczki, Alexandra Gyoengyoesi, Ildiko Bacskay, Miklos Vecsernyes, Judit Varadi
Summary: The antimicrobial effect of chitosan and its derivatives has been confirmed on various bacteria and fungi. The tests conducted on pathogenic microorganisms revealed the inhibitory effect mechanism and concentration dependence of chitosan. By adding commercially available chitosan derivatives to a probiotic strain, it was determined that chitosan has a viabilty decreasing effect at concentrations above the minimum inhibitory concentration (MIC). The study also identified increased cell membrane permeability and irreversible interaction between low molecular weight chitosan and bacterial cells.
Article
Food Science & Technology
Junyong Wang, Jing Zhang, Henan Guo, Qiang Cheng, Zaheer Abbas, Yucui Tong, Tiantian Yang, Yichen Zhou, Haosen Zhang, Xubiao Wei, Dayong Si, Rijun Zhang
Summary: The yield of exopolysaccharide (EPS) from Lactobacillus plantarum R301 was optimized using a single-factor experiment and response surface methodology (RSM), resulting in a 0.85-fold increase. The significant factors affecting EPS production were determined to be MgSO4 concentration, initial pH, and inoculation size. The EPS demonstrated strong antioxidant and anti-inflammatory activities, making it a potential multifunctional food additive in the food industry.
Article
Chemistry, Applied
Rebeca Penalva, Ana Luisa Martinez-Lopez, Carlos Gamazo, Carlos J. Gonzalez-Navarro, Carolina Gonzalez-Ferrero, Raquel Virto-Resano, Ana Brotons-Canto, Ana Isabel Vitas, Maria Collantes, Ivan Penuelas, Juan M. Irache
Summary: This study demonstrates the use of casein-chitosan microparticles to encapsulate Lactobacillus plantarum and their potential in targeting the distal areas of the gut and stimulating the immune system. The microparticles showed high protection and stability, and could reach the distal ileum and colon in vivo. Daily administration of the microparticles also resulted in enhanced immune responses.
FOOD HYDROCOLLOIDS
(2023)
Article
Food Science & Technology
Xuefang Zhao, Qi Liang
Summary: This study evaluated the effects of probiotic Lactobacillus plantarum MC5 on the quality, antioxidant activity, and storage stability of yogurt. The results showed that adding MC5 can improve the rheological properties, texture, and antioxidant activity of yogurt. The study also found that MC5 can promote the growth of Streptococcus thermophilus. Therefore, L. plantarum MC5 has potential for industrial application in milk fermentation.
Article
Biochemistry & Molecular Biology
Kippeum Lee, Hyeon Ji Kim, Soo A. Kim, Soo-Dong Park, Jae-Jung Shim, Jung-Lyoul Lee
Summary: This study explored the role of exopolysaccharides (EPS) produced by Lactobacillus plantarum HY7714 in regulating tight junctions in intestinal epithelial cells and increasing moisture retention in human dermal fibroblasts cells. The results demonstrated that HY7714 EPS effectively controlled intestinal tight junctions and improved UVB-induced cytotoxicity and hydration capacity in skin cells, showing its potential as an anti-aging molecule in skin and a therapeutic agent against skin diseases.
Article
Nutrition & Dietetics
Jie Yang, Yue Sun, Tengqi Gao, Yue Wu, Hao Sun, Qingzheng Zhu, Chunsheng Liu, Chuang Zhou, Yongbin Han, Yang Tao
Summary: This study shows that lactic acid bacteria can effectively ferment apple juice, resulting in increased viable cell counts, lactic acid content, and improved antioxidant and antibacterial capacities. After storage, the fermented apple juice still retains its antibacterial and in vitro antioxidant activities.
FRONTIERS IN NUTRITION
(2022)
Article
Microbiology
Muhammad Saleem, Samiullah Malik, Hafiza Mahreen Mehwish, Muhammad Waqas Ali, Nazim Hussain, Mohsin Khurshid, Muhammad Shahid Riaz Rajoka, Yougen Chen
Summary: The study demonstrated that EPS produced by Lactobacillus Plantarum S123 has promising biological features, including antibacterial, antioxidant, water holding, oil holding, flocculation, and emulsifying activities. These findings suggest its potential use in functional food products.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Masafumi Noda, Narandalai Danshiitsoodol, Takemasa Sakaguchi, Keishi Kanno, Masanori Sugiyama
Summary: Lactobacillus plantarum SN35N secretes negatively charged acidic exopolysaccharide (EPS) which inhibits the catalytic activity of hyaluronidase and shows inhibitory effect against influenza virus and feline calicivirus, making it a promising lead for developing anti-inflammatory and antiviral substances.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Biochemistry & Molecular Biology
Sameh Samir Ali, Eman Elgibally, Maha A. Khalil, Jianzhong Sun, Abd El-Raheem R. El-Shanshoury
Summary: This study extracted an exopolysaccharide (EPS) from Azotobacter salinestris AZ-6, isolated from soil cultivated with leguminous plants. The EPS showed potential application value in the food industry and pharmaceutical applications due to its cytotoxicity against the MCF-7 tumor cell line, moderate cytotoxicity towards HepG-2 cell line, and potent antioxidant and antibacterial properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Yunpeng Xu, Hongxin Wang, Guangqing Mu, Xuemei Zhu
Summary: This study used a mixed starter to effectively degrade alpha(S)-casein in skimmed milk and reduce its antigenicity. Label-free quantitative proteomics showed that the mixed starter more efficiently degraded the epitopes of major milk allergens and influenced the digestion pattern of the fermented milk. Additionally, molecular simulation predicted that new epitopes produced during fermentation could induce an immune response.
Article
Biochemistry & Molecular Biology
Thanapon Charoenwongpaiboon, Karan Wangpaiboon, Prapasri Septham, Nittaya Jiamvoraphong, Surapol Issaragrisil, Rath Pichyangkura, Chanchao Lorthongpanich
Summary: This study investigates the physicochemical properties and bioactivity of levan synthesized by recombinant levansucrase. The use of ultrasonication alters the physical and chemical properties of levan, enhancing its antiproliferative activity and Toll-like receptor expression. Sonicated levan shows potential for the development of cancer therapies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Giulia Bisson, Clara Comuzzi, Elisa Giordani, Denise Poletti, Marta Boaro, Marilena Marino
Summary: This study characterized the chemical and biological properties of an exopolysaccharide EPS_B3 produced by Leuconostoc mesenteroides strain isolated from an Italian cheese. The EPS_B3 displayed antimicrobial, antibiofilm, bifidogenic, antioxidant, and DNA-protective activities. It showed potential applications in functional foods, especially for its antimicrobial activity and ability to improve the viability of bifidobacteria in probiotic foods. Further research is needed to assess its ability to reach the gastrointestinal tract intact.
CARBOHYDRATE POLYMERS
(2023)
Article
Food Science & Technology
Mengke Yao, Min Zhang, Tiantian Lai, Zhennai Yang
Summary: In this study, exopolysaccharide (EPS) produced by Lactiplantibacillus plantarum NMGL2 from traditional fermented dairy cheese was purified and characterized. The purified EPS had a low molecular weight and high degradation temperature, and exhibited a specific microstructure. Furthermore, the EPS demonstrated regulatory effects on the gut microbiota and showed potential applications in the food and health care industry.
Article
Chemistry, Applied
Gang Chen, Chenyu Wu, Xiaojie Chen, Zhennai Yang, Huqing Yang
Summary: The study found that high hydrostatic pressure treatment led to changes in the structure and immunoreactivity of BLG, with pressure affecting the IgE binding ability, but temperature and pressure increases promoting BLG unfolding.
Review
Food Science & Technology
Ge-Ge Hu, Jing Liu, Yi-Hui Wang, Zhen-Nai Yang, Hong-Bo Shao
Summary: In recent years, double protein dairy products have gained popularity in the market. These products are made by replacing parts of animal protein with plant protein and using specific production methods. Studies have found that these products have improved the functional properties and taste of plant protein. This review examines the current state of research on double protein dairy products and suggests strategies for utilizing plant protein in food applications.
Article
Biotechnology & Applied Microbiology
Dong Han, Hongmin Zhen, Xiaoyan Liu, Justyna Zulewska, Zhennai Yang
Summary: This study used both rRNA and gDNA sequencing on mouse cecal contents and found differences in microbial composition and functional predictions between the two methods. rRNA-derived sequencing improved identification capability of specific microorganisms and was more suitable for functional predictions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Zhang Yang, Zidan Gao, Zhennai Yang, Yifan Zhang, Hongqun Chen, Xuexia Yang, Xuming Fang, Yingwu Zhu, Jiayan Zhang, Fu Ouyang, Jun Li, Gang Cai, Yuan Li, Xiang Lin, Ruihan Ni, Chong Xia, Ruihua Wang, Xiaofang Shi, Lan Chu
Summary: This study found that Lactobacillus plantarum-derived extracellular vesicles (LEVs) could reduce apoptosis in ischemic neurons, potentially through the miR-101a-3p/c-Fos/TGF-beta axis. Additionally, has-miR-101-3p may be a potential marker for neurological recovery in ischemic stroke patients.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Food Science & Technology
Katarzyna Kielczewska, Aneta Dabrowska, Marika Magdalena Bielecka, Bogdan Dec, Maria Baranowska, Justyna Ziajka, Yang Zhennai, Justyna Zulewska
Summary: Milk enriched with casein and serum protein preparations can enhance the pH, viscosity, and heat stability of non-fermented milks. Whey protein-enriched milk received the highest scores in sensory evaluation.
Article
Microbiology
Abid Sarwar, Sam Al-Dalali, Tariq Aziz, Zhennai Yang, Jalal Ud Din, Ayaz Ali Khan, Zubaida Daudzai, Quratulain Syed, Rubina Nelofer, Nazif Ullah Qazi, Zhang Jian, Anas S. Dablool
Summary: The addition of S. boulardii and inulin in fermented yogurt resulted in higher antioxidant and volatile compounds, making it a favorable choice for consumers.
Review
Food Science & Technology
Dong Han, Justyna Zulewska, Ke Xiong, Zhennai Yang
Summary: This review discusses the concept and current research on synbiotics, which are a dietary mixture of probiotic microorganisms and prebiotic substrates. Studies using microbial cultures, cell lines, animal models, and human subjects have shown that these combinations have metabolic properties that can promote beneficial activities, making them potentially useful for improving human health and for therapeutic purposes. However, there are challenges to the widespread use of these combinations, such as practical application, inconsistent results, unclear mechanisms, and legal hurdles.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Tariq Aziz, Muhammad Naveed, Abid Sarwar, Syeda Izma Makhdoom, Muhammad Saad Mughal, Urooj Ali, Zhennai Yang, Muhammad Shahzad, Manal Y. Sameeh, Mashael W. Alruways, Anas S. Dablool, Abdulraheem Ali Almalki, Abdulhakeem S. Alamri, Majid Alhomrani
Summary: This study reveals the important role of Lactiplantibacillus plantarum 13-3 in improving the condition of the human digestive system, enhancing immunity, and serving as a potential probiotic and bacteriocin-producing strain in food processing. The stability and quality of its genome have been assessed, and its probiotic-producing components, secondary metabolites, phage prediction sites, pathogenicity, and carbohydrate-producing enzymes have been analyzed computationally. The study demonstrates that L. plantarum 13-3 is nonpathogenic with various important functions and plays a significant role in food processing.
Article
Materials Science, Multidisciplinary
Yang Fei, Zhennai Yang, Sobia Niazi, Gang Chen, Muhammad Adnan Nasir, Imran Mahmood Khan, Abdur Rehman, Rana Muhammad Aadil, Monica Trif, Viorica Cosier
Summary: Peptides have diverse bioactive functions and can be applied to various tissues and materials, expanding the possibilities for coatings and films. Enzymatic hydrolysis of beta-lactoglobulin (b-LG) assisted by high hydrostatic pressure (HHP) increases the hydrolysis degree of b-LG and reduces its immunoreactivity, making it suitable for practical applications in coatings and films.
Article
Nutrition & Dietetics
Ruokun Yi, Xin Zhou, Tongji Liu, Rui Xue, Zhennai Yang
Summary: LP-KFY02 can ameliorate low-fiber diet-induced constipation in mice by regulating intestinal flora, increasing serum levels of gut motility-related neuroendocrine factors, and improving intestinal mucosal barrier function and colon tissue damage.
FRONTIERS IN NUTRITION
(2022)
Article
Microbiology
Tariq Aziz, Xingyu Hu, Abid Sarwar, Muhammad Naveed, Muhammad Aqib Shabbir, Ayaz Ali Khan, Taqweem Ulhaq, Muhammad Shahzad, Yang Zhennai, Ashwag Shami, Manal Y. Sameeh, Sahar A. Alshareef, Manal Abdulbari Tashkandi, Rewaa S. Jalal
Summary: Sufficient intake of probiotics can improve digestion, protect against pathogens, and enhance immunity. Non-dairy probiotic alternatives have become popular due to milk allergies and lactose intolerance. This study evaluated the antioxidant and probiotic properties of oat and soy milk fermented with Lactiplantibacillus plantarum strains, and found promising probiotic, antibacterial, and antioxidant properties. These findings can contribute to the development of dairy-free probiotic replacements for individuals with dietary or allergic restrictions.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Min Zhang, Ji Wang, Zhennai Yang
Summary: This study demonstrated that the EPS produced by L. plantarum YW11 has potent immunomodulatory and antitumor activities, showing significant inhibition of tumor growth and protection against organ damages induced by tumor cells.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Hua Zhao, Zhe Zheng, Man Zhang, Yihui Wang, Min Zhang, Zhennai Yang
Summary: By optimizing both medium composition and fermentation conditions, the milk-clotting enzyme produced by Bacillus amyloliquefaciens GSBa-1 was successfully scaled up from a 0.25-L flask to a 5-L bioreactor and exhibited improved milk-clotting activity. Response surface methodology was used to optimize the growth conditions of GSBa-1 and kinetic models for microbial growth, product formation, and substrate consumption were constructed.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yihui Wang, Miao Cai, Hong Zeng, Hua Zhao, Min Zhang, Zhennai Yang
Summary: The study found that bromelain can hydrolyze casein to obtain the highest iron-chelating activity, and the casein peptide fraction (CPF) obtained through ultrafiltration has high iron-chelating activity. Further analysis showed that the chelation of iron with CPF led to better structural and thermal stability, less toxicity to cells, and better iron absorption compared to ferrous sulfate. Western blot analysis also confirmed the increased expression of iron transporter proteins.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2022)
