Article
Microbiology
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study examined the prevalence and antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. The results indicated a low prevalence but presence of drug-resistant strains, highlighting a potential public health risk associated with this pathogen in the area.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Pattarapon Phangwipas, Balamurugan Thangavel, Joong Ho Shin
Summary: Foodborne illnesses are a global health problem and it is important to accurately and rapidly identify foodborne pathogens. This study introduces an automatic device that uses a lateral flow immunoassay strip to detect foodborne pathogens in fresh lettuce. The device is operated using a smartphone application, allowing users to quantify the results. The device can detect Escherichia coli O157:H7 contamination in lettuce with as low as 5 x 10(4) bacteria per 10 g.
Article
Biotechnology & Applied Microbiology
Da-Young Jee, Seung-Yeon Cha, Jae-Won Ha
Summary: Low-energy X-ray treatment can effectively reduce internalized bacteria in leafy greens without affecting product quality.
JOURNAL OF FOOD SAFETY
(2023)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Yangjin Jung, Mengqi Guo, Jingwen Gao, Hyein Jang, Karl R. Matthews
Summary: This study compared the efficacy of commercially available antimicrobials and tap water in preventing cross-contamination during the soaking step of crisping. The results showed that electrolyzed water was the most effective in completely eliminating cross-contamination of foodborne pathogens.
FOOD QUALITY AND SAFETY
(2022)
Article
Food Science & Technology
Yangjin Jung, Mengqi Guo, Jingwen Gao, Hyein Jang, Karl R. Matthews
Summary: The study compared the efficacy of commercially available antimicrobials, including electrolyzed water, lactic acid and phosphoric acid-based, and citric acid-based, with tap water in preventing cross-contamination during the crisping process in a retail setting. The results showed that electrolyzed water was the most effective in reducing contamination.
FOOD QUALITY AND SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Yu Zhang, Hung-Hsin Huang, Luyan Z. Ma, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
Summary: The study evaluated the combined effects of phage FP43 and mild heated slightly acidic hypochlorous water (SAHW) in reducing established biofilms on lettuce contaminated with Escherichia coli O157:H7. The sequential treatment of phage and SAHW significantly decreased the number of adhered cells, but did not completely remove the biofilms. Bacterial regrowth was observed after treatment with SAHW alone. The combination of phage and SAHW can be considered as a promising strategy to control the formation of E. coli O157:H7 biofilms on lettuce.
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Food Science & Technology
Kai Dong, Yuwan Li, Sijia Luo, Fan Zhang, Hanxu Pan, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: The study analyzed the sterilizing effects of different HHP-boosting processes on Escherichia coli O157:H7, finding that linear and fast boosting had the most significant bactericidal effects. The proteins interacting with GroEL under different boost modes and rates were involved in 42 metabolic pathways, particularly ribosome, ABC transporter, oxidative phosphorylation, sulfate assimilation, and cysteine biosynthesis. Key interacting proteins NuoB, NuoF, CysD, ZnuC, and RplX were identified and their interactions with GroEL were verified in vivo.
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Manman Du, Jinbiao Ma, Zhiwei Zhang, Guangzu Wu, Jianguo Wu, Hao Wang, Xinwu Xie, Can Wang
Summary: This study developed a rGO-FET biosensor for the rapid and sensitive detection of pathogenic microorganisms. The biosensor demonstrated good specificity, a wide linear range, and the ability to directly detect real samples without pretreatment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Jiye Li, Jie Wang
Summary: This study demonstrates the use of extinction spectrum to quickly transform the signal of MoS2-NS aggregation and reveals the size-dependent extinction response. Additionally, the size-dependent optical extinction behavior of MoS2-NSs with aptamer-induced dispersion is studied to improve target identification ability. This approach has been successfully employed for label-free detection of Escherichia coli O157:H7.
Article
Biotechnology & Applied Microbiology
Maria Mayara de Souza Grilo, Geany Targino de Souza Pedrosa, Ruthchelly Tavares da Silva, Fernanda Bovo Campagnollo, Donald W. Schaffner, Marciane Magnani
Summary: This study assessed the transfer of norovirus surrogate bacteriophage MS2 from different surfaces to raspberry and pitanga fruits, and evaluated the effect of sanitization and preservation technologies on MS2 survival. The results showed that glass and stainless steel surfaces had the highest viral transfer to raspberry, while glass and polypropylene surfaces had the highest transfer to pitanga. Sodium hypochlorite reduced MS2 titer in both fruits, and thermal treatments reduced MS2 titer in fruit pulps. These findings can inform the management of norovirus risk in fruit processing and handling.
Article
Biotechnology & Applied Microbiology
Matthew J. Igo, Laura K. Strawn, Donald W. Schaffner
Summary: This study analyzed 29 peer-reviewed journal articles to investigate the factors affecting the growth of Listeria monocytogenes on fresh produce. The results showed that factors such as incubation temperature, inoculum concentration, and produce surface characteristics significantly influenced the growth rate of L. monocytogenes. Future research should consider the impact of initial inoculum concentration when conducting growth studies on whole produce.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Biotechnology & Applied Microbiology
Anne-Laure Moyne, Joy Waite-Cusic, Linda J. Harris
Summary: The study found that spraying contaminated water on onions may pose a potential risk of E. coli growth, while inoculation through drip irrigation can reduce the contamination rate of bulb onions to a low level during harvest.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Microbiology
Matthew J. Igo, Donald W. Schaffner
Summary: Here, we present the draft genome sequence of a strain of Klebsiella michiganensis initially identified as Enterobacter aerogenes B199A. This strain has been utilized as a surrogate for Salmonella to investigate handwashing effectiveness and measure cross-contamination to and from various surfaces and foods.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biotechnology & Applied Microbiology
Jiin Jung, Donald w. Schaffner
Summary: This study quantifies the transfer of Salmonella Newport from the surface of cucumber to the edible flesh portion or peeler during peeling and evaluates Enterobacter aerogenes B199A, as well as native mesophilic microbiota, as surrogates for Salmonella transfer. The results show that during peeling, most of the pathogens or their surrogates remain associated with the peeler, and the transfer to cucumber flesh is relatively low.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Biotechnology & Applied Microbiology
Jiin Jung, Donald W. Schaffner
Summary: Baking wheat flour in a household toaster oven can be an effective method to inactivate pathogenic bacteria, despite its low water activity.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Biotechnology & Applied Microbiology
Jiin Jung, Donald W. Schaffner
Summary: This study developed mathematical models to predict the growth of Salmonella on sliced cucumbers under different temperature and relative humidity conditions. The results showed that temperature had an impact on the growth rate of Salmonella, while relative humidity did not. These models will be useful for future microbial risk assessments and predictions of Salmonella behavior on sliced cucumbers.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Food Science & Technology
Jiin Jung, Linda J. Harris
Summary: The addition of lactic acid or peracetic acid during tempering reduces the population of Salmonella or STEC on wheat berries.
Article
Biotechnology & Applied Microbiology
Vanessa Lieberman, Erika M. Estrada, Maeve Swinehart, Yaohua Feng, Linda J. Harris
Summary: By reviewing recipes from online blogs and YouTube videos, typical consumer handling practices for soaked walnut kernels were identified. The fate of foodborne pathogens during soaking and subsequent drying of walnut kernels was determined. The study found that the populations of E. coli, Listeria monocytogenes, and Salmonella increased significantly after 24 hours of soaking at 22 degrees Celsius. To reduce food safety risks, consumer education should emphasize sourcing treated walnuts, kitchen sanitation, hygiene measures, and shorter soaking times or soaking at cooler temperatures.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Plant Sciences
Xi Wu, Anne-laure Moyne, Thais De Melo Ramos, Linda J. Harris, Erin DiCaprio
Summary: The impact of water quality on the survival of human norovirus was studied in irrigation water field run-off and well water from a vegetable production site. The results showed that water quality did not significantly affect the stability of the virus, suggesting that human norovirus is highly stable in water.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Ann C. Vegdahl, W. Clifton Baldwin, Donald W. Schaffner
Summary: This study compared six growth models for three types of bacteria (Salmonella, pathogenic E. coli, and Listeria monocytogenes), and found that different models gave significantly different predictions for the same bacteria. However, the ability of the models to rank risk by truck was generally consistent.
Article
Biotechnology & Applied Microbiology
Yucen Xie, Nitin Nitin, Linda J. Harris
Summary: Factors influencing bacterial transfer between yellow onions and polyurethane or stainless steel surfaces under dry conditions were investigated. The study found that transfer rates were significantly dependent on bacterial species, inoculation levels, inoculum carrier, and transfer direction. The results showed that Rifampin-resistant Enterococcus faecium had higher transfer rates than Salmonella, and transfer from onion to polyurethane was higher than from polyurethane to onion.
Article
Biotechnology & Applied Microbiology
Erika M. Estrada, Anne-laure Moyne, Linda J. Harris
Summary: The genetic diversity of Salmonella isolates from pistachios collected in California was analyzed. Different serovars clustered together in the phylogenetic tree, suggesting a relationship between certain strains and the pistachio environment in California.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Biotechnology & Applied Microbiology
Hanna Louvau, Linda J. Harris
Summary: Raw cashews naturally contaminated with Salmonella were evaluated in relation to a salmonellosis outbreak. The study revealed that one box had four units contaminated with Salmonella while the other box had seven contaminated units. This research provides crucial information for hazard analysis and risk assessments of soaked and fermented cashew products.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Agriculture, Dairy & Animal Science
Shruthi L. Narasimhan, Deepti Salvi, Donald W. Schaffner, Mukund V. Karwe, Juzhong Tan
Summary: This study investigated a novel nonthermal sanitization technique, cold plasma-activated water (PAW), for washing shell eggs. Washing inoculated eggs with PAW achieved a similar microbial reduction as quaternary ammonia (QA) and did not adversely affect the shell structure or cuticle coverage. According to the results, PAW has the potential to be an alternative to commercial sanitizers like QA in the egg-washing industry.
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.