Article
Nanoscience & Nanotechnology
Shabir Hassan, Eduardo Gomez-Reyes, Eduardo Enciso-Martinez, Kun Shi, Jorge Gonzalez Campos, Oscar Yael Perez Soria, Eder Luna-Ceron, Myung Chul Lee, Isaac Garcia-Reyes, Joshua Steakelum, Haziq Jeelani, Luis Enrique Garcia-Rivera, Minsung Cho, Stephanie Sanchez Cortes, Tom Kamperman, Haihang Wang, Jeroen Leijten, Lance Fiondella, Su Ryon Shin
Summary: A combinational multimaterial and embedded bioprinting approach is used to develop implantable constructs that can maintain their structure in vivo. Self-healing and biodegradable colloidal gels are introduced as support baths to improve printing fidelity and provide suitable microenvironments for cell growth and infiltration.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Davide Ribezzi, Marieme Gueye, Sammy Florczak, Franziska Dusi, Dieuwke de Vos, Francesca Manente, Andreas Hierholzer, Martin Fussenegger, Massimiliano Caiazzo, Torsten Blunk, Jos Malda, Riccardo Levato
Summary: A novel technique called Embedded Extrusion-Volumetric Printing (EmVP) is introduced, combining extrusion bioprinting and layer-less, ultra-fast volumetric bioprinting. Light-responsive microgels are developed as bioresins for light-based volumetric bioprinting, providing a microporous environment for cell homing and self-organization. EmVP is used to create complex synthetic biology-inspired intercellular communication models, where adipocyte differentiation is regulated by optogenetic-engineered pancreatic cells.
ADVANCED MATERIALS
(2023)
Review
Materials Science, Multidisciplinary
Patrick Imrie, Jianyong Jin
Summary: Hydrogels have become essential materials for 3D printing in recent years, thanks to their printability, unique mechanical properties, network structure, and biocompatibility. The rise of multimaterial 3D printing has brought the field closer than ever to a new industrial revolution. This review investigates the use of hydrogels in multimaterial 3D printing, discussing various printing methods and applications, as well as advancements in hardware and inks.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Yongcong Fang, Yihan Guo, Bingyan Wu, Zibo Liu, Min Ye, Yuanyuan Xu, Mengke Ji, Li Chen, Bingchuan Lu, Kaiji Nie, Zixuan Wang, Jianbin Luo, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: A reversible ink template-based bioprinting strategy (SPIRIT) is developed to replicate the external geometry and internal structure of complex organs. This strategy utilizes a microgel-based bioink with shear-thinning and self-healing behavior to support embedded 3D printing, resulting in the generation of cardiac tissues and organoids. This technique allows for the rapid replication of complex organ geometry and internal structures, accelerating the biofabrication and therapeutic applications of tissue and organ constructs.
ADVANCED MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Annan Chen, Wanying Wang, Zhengyi Mao, Yunhu He, Shiting Chen, Guo Liu, Jin Su, Pei Feng, Yusheng Shi, Chunze Yan, Jian Lu
Summary: Additive manufacturing (AM) has significant advantages in the fabrication of tissue engineering implants. However, many tissue constructs have anisotropic heterogeneous structures, which pose challenges for conventional AM processes. Multimaterial 3D and 4D bioprinting offer a promising solution for constructing multifunctional implants with heterogeneous structures that can better mimic the host microenvironment.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jigang Huang, Henry Oliver T. Ware, Rihan Hai, Guangbin Shao, Cheng Sun
Summary: A new 3D printing process allows for full degree-of-freedom transformation of each individual building layer, enabling dynamic transformation without the need for time-consuming redesign steps.
ADVANCED MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Article
Engineering, Biomedical
Xin Liu, Xinhuan Wang, Liming Zhang, Lulu Sun, Heran Wang, Hao Zhao, Zhengtao Zhang, Wenli Liu, Yiming Huang, Shen Ji, Jingjinqiu Zhang, Kai Li, Biaobiao Song, Chun Li, Hui Zhang, Song Li, Shu Wang, Xiongfei Zheng, Qi Gu
Summary: This study presents a methodology for fabricating soft vascularized tissue at centimeter scale using multimaterial bioprinting with a customized multistage-temperature-control printer. The printed constructs can support 3D capillary networks to mimic mature and functional liver tissue, and also enable direct surgical anastomosis of blood vessels with the printed pressure-bearing layer. This versatile platform allows for optimal cellularization in engineered tissues through the replication of vasculature networks.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Multidisciplinary Sciences
Shangjing Xin, Kaivalya A. Deo, Jing Dai, Navaneeth Krishna Rajeeva Pandian, David Chimene, Robert M. Moebius, Abhishek Jain, Arum Han, Akhilesh K. Gaharwar, Daniel L. Alge
Summary: An in-depth investigation was conducted on the dissipation process of HMPs during printing, revealing that external resistance and internal physicochemical properties play a key role in the printing outcome. Understanding these mechanisms can help improve the printability of HMPs and enhance their use in 3D bioprinting.
Article
Chemistry, Physical
Mecit Altan Alioglu, Yasar Ozer Yilmaz, Yogendra Pratap Singh, Momoka Nagamine, Nazmiye Celik, Myoung Hwan Kim, Vaibhav Pal, Deepak Gupta, Ibrahim T. Ozbolat
Summary: In this study, a new method called 'Intra-Embedded Bioprinting (IEB)' is introduced, inspired by the Matryoshka doll. The capabilities of IEB are demonstrated in organ modeling, creating miniaturized replicas of a pancreas, a head phantom, and a Matryoshka doll. By using an innovative coupling strategy between extrusion-based and aspiration-assisted bioprinting, a breast tumor model is also successfully bioprinted. The method opens up new possibilities for bioprinting complex-shaped organs with internal anatomical features.
Article
Engineering, Biomedical
Simone Micalizzi, Lara Russo, Chiara Giacomelli, Francesca Montemurro, Carmelo de Maria, Martina Nencioni, Laura Marchetti, Maria Letizia Trincavelli, Giovanni Vozzi
Summary: Tendon and ligament injuries are common clinical problems with high failure rates after surgery. This study proposes a novel biofabrication approach using gradient-based scaffolds to engineer the enthesis, which is the area where tendons and ligaments insert into bones. The scaffolds were evaluated for biocompatibility and their ability to promote cellular adhesion, growth, proliferation, and differentiation. They were also morphologically and mechanically characterized, showing properties comparable to tendons and ligaments in vivo. This biofabrication approach has potential for clinical applications in tissue engineering.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biotechnology & Applied Microbiology
Bastian Boettcher, Astrid Pflieger, Jan Schumacher, Berit Jungnickel, Karl-Heinz Feller
Summary: Bioprinting allows the creation of complex three-dimensional cell laden grafts. However, nutrient transfer is a major challenge. Integrating tubular structures in the grafts can improve nutrient support. Prevascularized grafts show improvements in cell proliferation, especially when dynamically cultured, and the formation of vascular structures can be observed in co-cultures.
BIOENGINEERING-BASEL
(2022)
Article
Engineering, Biomedical
Heran Wang, Kai Guo, Liming Zhang, Huixuan Zhu, Shijie Li, Song Li, Feiyang Gao, Xin Liu, Qi Gu, Lianqing Liu, Xiongfei Zheng
Summary: Bioprinting is a promising technology for tissue manufacturing, focusing on precise assembly of multiple materials. The valve-based consecutive bioprinting (VCB) method presented in this study offers greater mechanical strength and printing efficiency compared to the extrusion-based multi-printhead bioprinting (MPB) method. The VCB method allows for seamless switching of materials at the interface, leading to the fabrication of heterogeneous tissues with complex multimaterial interfaces.
Article
Engineering, Biomedical
Philipp Fisch, Martin Holub, Marcy Zenobi-Wong
Summary: 3D bioprinting has experienced significant growth in various fields, with micro-extrusion bioprinting being the most commonly used method. Researchers have evaluated a novel extrusion technique based on a miniaturized progressive cavity pump (PCP) to improve accuracy and precision in bioink extrusion, achieving higher accuracy and precision compared to conventional pneumatic extrusion systems while maintaining good cell viability.
Article
Materials Science, Multidisciplinary
Yi Zhang, Piyush Kumar, Songwei Lv, Di Xiong, Hongbin Zhao, Zhiqiang Cai, Xiubo Zhao
Summary: 3D bioprinting technology combines computing science, biology, and material engineering, and has great potential in fabricating vascularized constructs for tissue engineering. This review summarizes recent advances in 3D bioprinting of vascularized tissues, including the selection of biomaterials and various 3D printing techniques and applications.
MATERIALS & DESIGN
(2021)
Review
Biochemistry & Molecular Biology
Samson Afewerki, Thiago Domingues Stocco, Andre Diniz Rosa da Silva, Andre Sales Aguiar Furtado, Gustavo Fernandes de Sousa, Guillermo U. Ruiz-Esparza, Thomas J. Webster, Fernanda R. Marciano, Maria Stromme, Yu Shrike Zhang, Anderson Oliveira Lobo
Summary: Precision medicine allows for tailored treatments, improving the efficiency and accuracy of disease prevention, diagnosis, and treatment. The combination of organ-on-a-chip and bioprinting can address precision medicine challenges and be utilized in the current COVID-19 pandemic for efficient diagnostics and treatments. This convergence enables advances in tools and in vitro models for personalized and faster screening of therapeutics.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Review
Materials Science, Multidisciplinary
Xuan Mu, Marie Denise Gerhard-Herman, Yu Shrike Zhang
Summary: Blood vessel chips are bioengineered microdevices that mimic essential vascular structure and physiology, allowing for controlled microenvironments and spatial-temporal readouts. They provide promising opportunities to study vascular diseases and offer insights into biological processes and disease pathogenesis. The physiological relevance is crucial and they rely on bioinspired strategies and engineering approaches to replicate vascular physiology.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Chemistry, Multidisciplinary
Carla Spatola Rossi, Frederic Coulon, Shaohua Ma, Yu Shrike Zhang, Zhugen Yang
Summary: Rapid, sensitive, and selective detection of live pathogens is crucial for quality control and risk assessment. Conventional methods are often complex, expensive, time-consuming, and not suitable for field testing or low-resource settings. Microfluidic devices that integrate laboratory functions in a miniaturized manner have emerged as a promising tool for rapid and sensitive pathogen detection.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Editorial Material
Engineering, Biomedical
Yu Shrike Zhang, Mario Moises Alvarez, Grissel Trujillo-de Santiago
Summary: The field of biofabrication has made significant advancements in the past decade, particularly in the generation of biomimetic models of human tissues to study healthy and diseased states. These models have broad applications in fundamental biology research and compound screening, including therapeutic agents. The United States FDA Modernization Act 2.0, which eliminates the requirement for animal tests before human drug trials, is expected to further accelerate the development of this field. This Special Issue emphasizes the latest developments in biofabrication for human disease modeling, including 3D printing, organ-on-a-chip, and their integration.
Article
Pharmacology & Pharmacy
Thuy Giang Nguyen Cao, Ji Hee Kang, Su Jin Kang, Quan Truong Hoang, Han Chang Kang, Won Jong Rhee, Yu Shrike Zhang, Young Tag Ko, Min Suk Shim
Summary: This study utilized brain endothelial cell-derived extracellular vesicles as a nano-carrier to transport photosensitizers into brain tumors, thereby enhancing the efficacy of photodynamic therapy for the treatment of malignant brain tumors.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Engineering, Biomedical
Marcel Alexander Heinrich, Irene Uboldi, Praneeth Reddy Kuninty, Marc J. K. Ankone, Joop van Baarlen, Yu Shrike Zhang, Kartik Jain, Jai Prakash
Summary: A multi-layered vascularized 3D PDAC model was developed to study the compression of blood vessels in pancreatic ductal adenocarcinoma (PDAC) caused by fibrosis. The model mimics the activation of cells and vessel compression observed in fibrotic tumors. In silico and in vivo experiments showed that cancer-associated fibroblast modulation therapy can inhibit the cell-mediated compression of blood vessels in PDAC.
BIOACTIVE MATERIALS
(2023)
Review
Chemistry, Analytical
Manna Dai, Gao Xiao, Ming Shao, Yu Shrike Zhang
Summary: Organs-on-chips (OoCs) are advanced in vitro models that are miniature microfluidic systems. Deep learning, an emerging topic in machine learning, can extract hidden statistical relationships from input data. This review discusses the integration of deep learning with OoCs for drug screening and presents successful use cases. The future prospects, perspectives, and potential challenges of combining OoCs and deep learning for image processing and automation designs are also discussed.
Review
Biochemistry & Molecular Biology
Berivan Cecen, Shabir Hassan, Xin Li, Yu Shrike Zhang
Summary: Smart biomaterials that can alter their properties in response to external stimuli or environmental changes have gained significant attention in the biomedical community. Shape-memory materials, in particular, have been extensively studied for their potential biomedical applications, offering unique structural reconfiguration features and enhanced biocompatibility.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Engineering, Biomedical
Gang Ren, Xia Zhou, Ruimin Long, Maobin Xie, Ranjith Kumar Kankala, Shibin Wang, Yu Shrike Zhang, Yuangang Liu
Summary: In this review, the formation mechanism of magnetosomes and various modification methods are discussed. The biomedical advancements of bacterial magnetosomes in biomedical imaging, drug delivery, anticancer therapy, and biosensors are presented. Future applications and challenges are also discussed. This review summarizes the application of magnetosomes in the biomedical field, highlighting the latest advancements and exploring the future development.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Philipp Weber, Ling Cai, Francisco Javier Aguilar Rojas, Carlos Ezio Garciamendez-Mijares, Maria Celeste Tirelli, Francesco Nalin, Jakub Jaroszewicz, Wojciech Swieszkowski, Marco Costantini, Yu Shrike Zhang
Summary: Three-dimensional (3D) printing has been successful in engineering human tissues, and the integration of a digital light processing (DLP) printing procedure with a microfluidic chip system allows for the production of size-tunable, 3D-printable porosities within a GelMA hydrogel matrix.
Article
Multidisciplinary Sciences
Yunhua Wang, Guoxia Zheng, Nan Jiang, Guoliang Ying, Yiwei Li, Xiaolu Cai, Jiashen Meng, Liqiang Mai, Ming Guo, Yu Shrike Zhang, Xingcai Zhang
Summary: This article discusses the importance of nature inspiration in micropatterned materials and presents the key principles, fabrication methods, and applications of nature-inspired micropatterns. By imitating natural strategies, engineering challenges can be solved and novel functions can be imparted to materials. Nature inspiration is an interdisciplinary field involving various materials and fabrication techniques.
NATURE REVIEWS METHODS PRIMERS
(2023)
Article
Materials Science, Biomaterials
Marcel Alexander Heinrich, Lena Heinrich, Marc J. K. Ankone, Bjorn Vergauwen, Jai Prakash
Summary: Recent developments in biofabrication, especially 3D bioprinting, have highlighted the need for biomaterials that can accurately replicate target tissues. However, the presence of endotoxins in these biomaterials is often overlooked. This study demonstrates that high levels of endotoxins in commercially-available gelatin can significantly impact the behavior of macrophages and cancer cells in a 3D bioprinted co-culture model. The contamination of biomaterials with endotoxins can affect intra- and intercellular communication and drug efficacy, potentially leading to misinterpretation of the tested compounds' potency and safety.
BIOMATERIALS ADVANCES
(2023)
Article
Multidisciplinary Sciences
Riccardo Levato, Oksana Dudaryeva, Carlos Ezio Garciamendez-Mijares, Bruce E. Kirkpatrick, Riccardo Rizzo, Jacob Schimelman, Kristi S. Anseth, Shaochen Chen, Marcy Zenobi-Wong, Yu Shrike Zhang
Summary: Light-based vat-polymerization bioprinting allows computer-aided patterning of 3D cell-laden structures using photoactivatable bioresin in vats. This technology, divided into stereolithography, digital light processing and volumetric additive manufacturing, has been widely developed and applied in biomedicine. This Primer introduces the methodology and variations of light-based vat-polymerization 3D bioprinting, as well as discusses key assessments and future directions of this method.
NATURE REVIEWS METHODS PRIMERS
(2023)
Review
Chemistry, Multidisciplinary
Sajjad Rahmani Dabbagh, Misagh Rezapour Sarabi, Mehmet Tugrul Birtek, Nur Mustafaoglu, Yu Shrike Zhang, Savas Tasoglu
Summary: Organ-on-a-chip (OOC) platforms provide a more realistic environment compared to animal models and conventional cell cultures, offering continuous perfusion of cell cultures, integration of biosensors, and 3D structures fabricated through bioprinting. These platforms have great potential for understanding organ functions, studying disease impacts, and drug screening. This review presents an overview of bioprinting techniques, their advantages and limitations, recent advancements, applications, and future prospects of the 3D-bioprinted OOC platforms, as well as current challenges and future directions.