Article
Engineering, Biomedical
Liliang Ouyang, Jonathan P. Wojciechowski, Jiaqing Tang, Yuzhi Guo, Molly M. Stevens
Summary: This study introduces a method to engineer controlled microporosity in 3D bioprinted hydrogels using microgel-templated porogel (MTP) bioinks. By fabricating templated gelatin microgels with different sizes and mixing them with photo-crosslinkable formulations, the researchers achieved consistent printability and the formation of micropores in situ. This approach provides a facile strategy to compensate for the limited resolution of current bioprinting approaches and engineer precise micropores in 3D printed structures.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Analytical
Bo Yang, Tianqi Liu, Ge Gao, Xianglin Zhang, Bin Wu
Summary: This study explores the GelMA/Agarose suspension printing system and defines a suitable printing window. The resulting scaffolds have good water absorption and elasticity but exhibit significant deformation during printing.
Article
Chemistry, Multidisciplinary
Malin Becker, Melvin Gurian, Maik Schot, Jeroen Leijten
Summary: Embedded 3D bioprinting using low-viscosity ink 3D (LoV3D) bioprinting based on aqueous two-phase stabilization allows for the fabrication of living constructs at high speeds with high viability. The liquid/liquid interfaces of LoV3D bioprinting offer unique advantages for fusing structures, creating vasculature, and modifying surfaces. The low interfacial tension of LoV3D bioprinting allows for nozzle-independent control over filament diameter, enabling the printing of a wide range of diameters down to the width of a single cell.
Article
Nanoscience & Nanotechnology
Yongcong Fang, Mengke Ji, Bingyan Wu, Xinxin Xu, Ge Wang, Yanmei Zhang, Yingkai Xia, Zhe Li, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: The study developed a GAP bioink for engineering highly vascularized bone tissues using prevascularized mesenchymal spheroids. The prevascularized spheroids formed an interconnected vascular network through angiogenic sprouting after printing. The results showed that prevascularized spheroids were more conducive to osteogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Engineering, Biomedical
Chengcheng Du, Wei Huang, Yiting Lei
Summary: Three-dimensional (3D) bioprinting technology is an advanced technique in tissue engineering and regenerative medicine. Microgels, a new type of bioink, have adjustable pore structures and excellent mechanical and biological properties, making them great candidates for 3D bioprinting applications. This review discusses the application and future development of microgels in biomedicine and 3D bioprinting.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Chemistry, Multidisciplinary
Alessandra Dellaquila, Chau Le Bao, Didier Letourneur, Teresa Simon-Yarza
Summary: The study discusses the importance of vascularization techniques in tissue engineering and the current research progress. By using different strategies and materials, more biologically relevant and organ-specific 3D models can be established, which are expected to be used for regenerative medicine and drug development in the future.
Article
Engineering, Biomedical
Yongcong Fang, Mengke Ji, Yi Yang, Yihan Guo, Ruobin Sun, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: Three-dimensional bioprinting has been hindered by a lack of suitable bioinks for printing functional tissues. We developed a granular cell aggregate-based biphasic (GCAB) bioink that exhibited desired properties for extrusion bioprinting and modeling the mechanical characteristics of soft tissues. By preorganizing GCAB bioink and printing it with an endothelial cell-laden gelatin bioink, we successfully created functional hepatic tissues with high cell density and a perfusable vascular network. This GCAB bioink design has potential for creating functional tissues for therapeutic applications.
Article
Chemistry, Multidisciplinary
Zeyu Luo, Liming Lian, Thiago Stocco, Jie Guo, Xuan Mei, Ling Cai, Syed Muntazir Andrabi, Yajuan Su, Guosheng Tang, Hossein Ravanbakhsh, Wanlu Li, Mian Wang, Xiao Kuang, Carlos Ezio Garciamendez-Mijares, Di Wang, Zongliang Wang, Junlong Liao, Maobin Xie, Jingwei Xie, Heemin Kang, Anderson Oliveira Lobo, Zongke Zhou, Yu Shrike Zhang
Summary: A cryo(bio)printing-based method is developed to fabricate shelf-ready, storable modules for scalable tissue assembly. The method involves assembling cryo(bio)printed modular scaffolds in their frozen state and subsequently bonding them together into an integral piece during the melting and photocrosslinking processes. This method overcomes the height limitation of cryo(bio)printing and allows the generation of tissue constructs of same or dissimilar materials for more precise treatment.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Isabel Orellano, Alexander Thomas, Aaron Herrera, Erik Brauer, Dag Wulsten, Ansgar Petersen, Lutz Kloke, Georg N. Duda
Summary: The research introduces a bioengineering approach that utilizes 3D bioprinting to control the formation of microvascular structures by guiding cellular self-assembly. The constructed vascular networks display regulated distribution, network orientation, vessel length and branching behavior, as well as developed lumens and signs of vascular stabilization. This novel biofabrication approach shows the capability to generate distinctly vascularized constructs with controlled microvascular network formation, which could be significant in the development of vascularized implants or in vitro screening models.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Ariel A. Szklanny, Majd Machour, Idan Redenski, Vaclav Chochola, Idit Goldfracht, Ben Kaplan, Mark Epshtein, Haneen Simaan Yameen, Uri Merdler, Adam Feinberg, Dror Seliktar, Netanel Korin, Josef Jaros, Shulamit Levenberg
Summary: Creating engineered hierarchical vasculatures is crucial for implantable functional thick tissues. This study introduces a combined approach using millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues to achieve fully engineered hierarchical vascular constructs for implantation. The use of sacrificial molds, endothelial cells, and vascularizing hydrogels enables the formation of functional vessels within the engineered tissue, promoting tissue perfusion and host vasculature ingrowth.
ADVANCED MATERIALS
(2021)
Article
Engineering, Biomedical
Gagan K. Jalandhra, Thomas G. Molley, Tzong-tyng Hung, Iman Roohani, Kristopher A. Kilian
Summary: This study developed a unique printing platform that integrates soft and hard materials concurrently through freeform printing of mineralized constructs within tunable micro-gel suspensions containing living cells. By tuning the microgel stiffness and filler content, chondrogenesis and osteogenesis can be differentially directed within the same construct, enabling the fabrication of osteochondral interfaces in a single step. This versatile one-pot biofabrication approach has the potential to aid in bone disease modeling and tissue engineering.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Xin Liu, Xinhuan Wang, Liming Zhang, Lulu Sun, Heran Wang, Hao Zhao, Zhengtao Zhang, Wenli Liu, Yiming Huang, Shen Ji, Jingjinqiu Zhang, Kai Li, Biaobiao Song, Chun Li, Hui Zhang, Song Li, Shu Wang, Xiongfei Zheng, Qi Gu
Summary: This study presents a methodology for fabricating soft vascularized tissue at centimeter scale using multimaterial bioprinting with a customized multistage-temperature-control printer. The printed constructs can support 3D capillary networks to mimic mature and functional liver tissue, and also enable direct surgical anastomosis of blood vessels with the printed pressure-bearing layer. This versatile platform allows for optimal cellularization in engineered tissues through the replication of vasculature networks.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Review
Chemistry, Multidisciplinary
Maurice N. Collins, Guang Ren, Kieran Young, S. Pina, Rui L. Reis, J. Miguel Oliveira
Summary: Bone tissue engineering aims to create biofunctional tissue for treating diseases, with scaffold fabrication techniques and biomaterial selection being crucial. Vascularization and immunomodulation are major challenges in this field.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
Yang Wu, Ming Li, Hao Su, Huaying Chen, Yonggang Zhu
Summary: This review discusses the major considerations, challenges, and potential strategies for bone bioprinting, and summarizes the latest progress in this field. It uncovers the immense potential of bioprinting in re-establishing the 3D dynamic microenvironment of the native bone, and can assist researchers in reconstructing clinically relevant bone tissues with appropriate mechanical properties and precisely regulated biological behaviors.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Letter
Biochemistry & Molecular Biology
Yubing Liu, Xinmei Lu, Min Ye, Ling Wang, Rongxin Tang, Zhiyong Yang, Bongkoch Turathum, Chenchen Liu, Yujing Xue, Menghua Wu, Yannan Yang, Ermeng Gao, Di Zhang, Feng Yang, Keh-Kooi Kee, Xingxu Huang, Guanglei Li, Ri-Cheng Chian
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Rehabilitation
Zi-Bo Liu, Lu-yi Wang, Long Zhao, Ya-Tao Pang, Yan-song Liu, Wei Xu, Hong-Ling Li
Summary: This study investigated the effect of pulmonary rehabilitation combined with diaphragm pacemaker therapy on severely ill mechanically ventilated patients. The results showed that this combined treatment improved physiological scores, reduced diaphragm mobility and thickness, and shortened hospital stays. This treatment approach is safe and effective.
INTERNATIONAL JOURNAL OF REHABILITATION RESEARCH
(2022)
Article
Behavioral Sciences
Yan-Song Liu, Zi-bo Liu, Zhe Yang, Long Zhao, Hong-Ling Li
Summary: The study demonstrated that hyperbaric oxygen combined with RMNES can improve the state of consciousness and promote the recovery of consciousness for patients with consciousness disorders caused by brain injury. The clinical efficacy of test group 2 was higher than that of test group 1 and the control group.
BRAIN AND BEHAVIOR
(2022)
Review
Physiology
Qianglin Liu, Anqi Zeng, Zibo Liu, Chunjie Wu, Linjiang Song
Summary: The liver, as the largest internal organ, plays a crucial role in multiple physiological processes. Previous liver research was mainly conducted on animal models and cell lines, lacking in species variability and heritable material retention. Primary hepatocytes also struggle to maintain metabolic functions after in vitro expansion. With the global burden of liver diseases increasing, 3D in vitro liver models, known as liver organoids, are in high demand. Liver organoid generation relies on various initiation cells and eventually forms three-dimensional structures in the presence of cytokines. Liver organoids have shown promising applications in drug screening, disease modeling, and individualized medicine. This review summarizes the generation process and clinical applications of liver organoids, providing new perspectives for liver physiology and disease research.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Medicine, Research & Experimental
Xun Ye, Jinhu Li, Zibo Liu, Xue Sun, Daneng Wei, Linjiang Song, Chunjie Wu
Summary: Fibrosis is a disease characterized by excessive accumulation of extracellular matrix, which can lead to organ failure and death. Activated myofibroblasts have been identified as the main cellular elements responsible for the replacement of normal tissues with nonfunctional fibrotic tissue. The transforming growth factor-beta pathway plays a significant role in fibrosis. Although there is no specific drug for fibrosis treatment, various peptides have shown promise in anti-fibrosis therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Physical
Yujin Wang, Haolei Dai, Zibo Liu, Dameng Liu
Summary: Monolayer molybdenum disulfide (MoS2), a two-dimensional transition-metal dichalcogenide material, is used extensively in various industries due to its unique material properties compared to its bulk form. This study investigates the effects of temperature and defect concentration on phonon scattering in MoS2, using temperature-dependent Raman spectroscopy. A predictive model is then developed to estimate phonon lifetime under different defect concentrations, providing a useful tool for defect/photon engineering. The study contributes to a better understanding of MoS2's phonon behavior and its potential applications.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Engineering, Biomedical
Bingchuan Lu, Min Ye, Jingjing Xia, Zhenrui Zhang, Zhuo Xiong, Ting Zhang
Summary: The formation of multiscale vascular networks is essential for the construction of large-scale biomimetic cardiac tissues/organs in vitro. Electrical stimulation is applied to promote the elongation and connection of endothelial cells in engineered cardiac tissue (ECT) and achieve vascularization. The results show that electrical stimulation significantly enhances the elongation, migration, and interconnection of endothelial cells in ECT and increases the expression of related genes. Electrical stimulation also enhances the secretion of signal factors between cardiomyocytes and endothelial cells and contributes to the contractility of cardiac tissue.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yongcong Fang, Chengjin Wang, Zibo Liu, Jeonghoon Ko, Li Chen, Ting Zhang, Zhuo Xiong, Lei Zhang, Wei Sun
Summary: In this study, a conductive multiscale filled NGC (MF-NGC) is developed, which significantly enhances peripheral nerve regeneration. The printed MF-NGCs possess good permeability, mechanical stability, and electrical conductivity, promoting the elongation and growth of Schwann cells and neurite outgrowth. Animal studies demonstrate that the MF-NGCs promote neovascularization and M2 transition, leading to improved peripheral nerve regeneration. This study demonstrates the feasibility of using 3D-printed conductive MF-NGCs with hierarchically oriented fibers as functional conduits.
Article
Biotechnology & Applied Microbiology
Chuqiao Pan, Peng Xu, Yi Zheng, Yikai Wang, Chuhsin Chen, Shibo Fu, Zibo Liu, Yahong Chen, Ke Xue, Qimin Zhou, Kai Liu
Summary: A study found that therapeutic extracellular vesicles (EVs) can be extracted from adipose tissue using a mechanical method, which has potential application in treating diabetic wounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Min Ye, Yiran Shan, Bingchuan Lu, Hao Luo, Binhan Li, Yanmei Zhang, Zixuan Wang, Yuzhi Guo, Liliang Ouyang, Jin Gu, Zhuo Xiong, Ting Zhang
Summary: The bio-engineered ovary is a crucial technology for treating female infertility. The development of in vitro models, such as the micro-cavity ovary (MCO), provides a promising approach for studying drug effects. The MCO can support follicle growth and mimic ovarian functions, making it suitable for investigating ovarian toxicity and potential clinical research in the future.
BIOACTIVE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Yi Zheng, Peng Xu, Chuqiao Pan, Yikai Wang, Zibo Liu, Yahong Chen, Chuhsin Chen, Shibo Fu, Ke Xue, Qimin Zhou, Kai Liu
Summary: Low-intensity ultrasound stimulation enhances the secretion of extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) for diabetic wound treatment. The study identifies the optimal ultrasound stimulation parameter that significantly increases the quantity of ADSC-EVs, which are enriched with wound healing-related miRNAs. Furthermore, these ultrasound-stimulated EVs promote cellular functions and facilitate diabetic wound healing through various processes in vitro and in vivo.
STEM CELL REVIEWS AND REPORTS
(2023)