Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Review
Materials Science, Multidisciplinary
Patrick Imrie, Jianyong Jin
Summary: Hydrogels have become essential materials for 3D printing in recent years, thanks to their printability, unique mechanical properties, network structure, and biocompatibility. The rise of multimaterial 3D printing has brought the field closer than ever to a new industrial revolution. This review investigates the use of hydrogels in multimaterial 3D printing, discussing various printing methods and applications, as well as advancements in hardware and inks.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Engineering, Biomedical
Xin Liu, Xinhuan Wang, Liming Zhang, Lulu Sun, Heran Wang, Hao Zhao, Zhengtao Zhang, Wenli Liu, Yiming Huang, Shen Ji, Jingjinqiu Zhang, Kai Li, Biaobiao Song, Chun Li, Hui Zhang, Song Li, Shu Wang, Xiongfei Zheng, Qi Gu
Summary: This study presents a methodology for fabricating soft vascularized tissue at centimeter scale using multimaterial bioprinting with a customized multistage-temperature-control printer. The printed constructs can support 3D capillary networks to mimic mature and functional liver tissue, and also enable direct surgical anastomosis of blood vessels with the printed pressure-bearing layer. This versatile platform allows for optimal cellularization in engineered tissues through the replication of vasculature networks.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Annan Chen, Wanying Wang, Zhengyi Mao, Yunhu He, Shiting Chen, Guo Liu, Jin Su, Pei Feng, Yusheng Shi, Chunze Yan, Jian Lu
Summary: Additive manufacturing (AM) has significant advantages in the fabrication of tissue engineering implants. However, many tissue constructs have anisotropic heterogeneous structures, which pose challenges for conventional AM processes. Multimaterial 3D and 4D bioprinting offer a promising solution for constructing multifunctional implants with heterogeneous structures that can better mimic the host microenvironment.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Applied
Sushma Kumari, Pritiranjan Mondal, Kaushik Chatterjee
Summary: This study demonstrates the excellent printability of photocurable methacrylate-kappa-carrageenan (MA-kappa-CA) in DLP 3D printing and its suitability for bioprinting highly complex 3D cell-laden scaffolds. Through thorough characterization of its physical and biological properties, this bioink has shown potential as a material for tissue scaffold fabrication.
CARBOHYDRATE POLYMERS
(2022)
Article
Materials Science, Multidisciplinary
Chen Qin, Jingge Ma, Lei Chen, Hongshi Ma, Hui Zhuang, Meng Zhang, Zhiguang Huan, Jiang Chang, Nan Ma, Chengtie Wu
Summary: This study successfully developed a multicellular system based on a bilayered co-culture scaffold for osteochondral regeneration via a 3D bioprinting strategy. The results show the potential of inducing osteochondral tissue regeneration in vivo through the 3D bioprinted co-culture scaffolds.
Article
Engineering, Manufacturing
Renan Roque, Gustavo Franco Barbosa, Antonio Carlos Guastaldi
Summary: This study focused on the design and manufacturing of porous scaffolds using 3D printing technology, aiming to create complex 3D structures with suitable mechanical properties for bone regeneration. The results showed that the designed scaffolds met the required specifications for rigidity and compressive strength, making them a promising innovation for tissue engineering applications.
JOURNAL OF MANUFACTURING PROCESSES
(2021)
Article
Biotechnology & Applied Microbiology
Suihong Liu, Qingxi Hu, Zhipeng Shen, Sasirekha Krishnan, Haiguang Zhang, Murugan Ramalingam
Summary: The study demonstrated the suitability of a 3D printable formulation for self-standing and vascular supportive structures, which has great potential in organ engineering. The formulated hydrogel showed excellent biocompatibility and vascular supportive behavior, making it possible to manufacture organs with complex shapes and structures.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Materials Science, Multidisciplinary
Yi Zhang, Piyush Kumar, Songwei Lv, Di Xiong, Hongbin Zhao, Zhiqiang Cai, Xiubo Zhao
Summary: 3D bioprinting technology combines computing science, biology, and material engineering, and has great potential in fabricating vascularized constructs for tissue engineering. This review summarizes recent advances in 3D bioprinting of vascularized tissues, including the selection of biomaterials and various 3D printing techniques and applications.
MATERIALS & DESIGN
(2021)
Review
Chemistry, Analytical
Shebin Tharakan, Shams Khondkar, Azhar Ilyas
Summary: Bioprinting stem cells into 3D scaffolds offers great potential in regenerative medicine, bone tissue engineering, and biosensor manufacturing, impacting cellular behavior and differentiation. Different printing methods have varying effects on stem cell viability, proliferation, and differentiation potential. The use of suitable biomaterials and printing methods can enhance osteogenesis and promote advancements in bone tissue engineering.
Article
Biotechnology & Applied Microbiology
Christopher David Roche, Poonam Sharma, Anthony Wayne Ashton, Chris Jackson, Meilang Xue, Carmine Gentile
Summary: This study evaluated 3D bioprinted cardiac patches using AlgGel hydrogels, finding key factors affecting durability included the number of layers, movement disturbance during culture, and the addition of extracellular matrix. The cells in the patches remained viable after bioprinting and during culture, with endothelial cells able to self-organize into network-like structures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Chen Qin, Hongjian Zhang, Lei Chen, Meng Zhang, Jingge Ma, Hui Zhuang, Zhiguang Huan, Yin Xiao, Chengtie Wu
Summary: To achieve regeneration of highly vascularized and innervated tissues, a pre-angiogenic cell-laden scaffold with durable angiogenic functions is prepared. The scaffold supports long-term cell survival and growth, and exhibits durable angiogenic capability both in vitro and in vivo. The pre-angiogenic scaffolds can induce the neurogenetic differentiation and osteogenic differentiation, and may represent the future direction of biomaterials for complex tissue/organ regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Jiaqi Xu, Manyue Zhang, Wenzhen Du, Jiuhong Zhao, Guixia Ling, Peng Zhang
Summary: The emerging 3D bioprinting technology has brought hope for the development of tissue engineering and regenerative medicine. Chitosan-based supramolecular hydrogels are considered as ideal materials for 3D bioprinting. However, the mechanical properties of these hydrogels still require further research and exploration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Yuan -Zhu Xin, Xiaoying Li, Seok-Jo Yang, JunHee Lee, Chunbao Liu, Yuqiang Fang
Summary: Scaffolds are crucial for tissue engineering, with their mechanical properties significantly impacting the success rate of regeneration. This study developed semi-analytical solutions to accurately calculate the mechanical properties of porous structures.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Maria C. Teixeira, Nicole S. Lameirinhas, Joao P. F. Carvalho, Bruno F. A. Valente, Jorge Luis, Liliana Pires, Helena Oliveira, Martinho Oliveira, Armando J. D. Silvestre, Carla Vilela, Carmen S. R. Freire
Summary: In this study, alginate nanocomposite hydrogel bioinks reinforced with lysozyme nanofibers were developed. The bioinks showed improved rheological performance, printability, and biological properties, making them a promising solution to overcome the limitations of alginate-based bioinks. The optimized A-LNF inks exhibited good shear-thinning behavior, recovery properties, and noncytotoxicity, providing a solid foundation for bioprinting applications.
Review
Biochemistry & Molecular Biology
Jinghang Li, Xin Yu, Eduardo Enciso Martinez, Jiaqing Zhu, Ting Wang, Shengwei Shi, Su Ryon Shin, Shabir Hassan, Chengchen Guo
Summary: The development of bioadhesives using naturally derived biopolymers has addressed challenges and made significant progress, leading to their wide applications in healthcare and biomedical fields.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Niels G. A. Willemen, Shabir Hassan, Melvin Gurian, Maria Fernanda Jasso-Salazar, Kai Fan, Haihang Wang, Malin Becker, Iris E. Allijn, Ayca Bal-Ozturk, Jeroen Leijten, Su Ryon Shin
Summary: Oxygen releasing biomaterials can enhance the survival of living implants by releasing physiologically relevant amounts of oxygen. Hydrophobic oxygen generating microparticles containing calcium peroxide have been used in tissue engineering to release oxygen, but they generate toxic hydrogen peroxide. This study explores the most effective formulation for incorporating antioxidants to scavenge hydrogen peroxide in oxygen generating biomaterials.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Biomedical
Kamil Elkhoury, Mo Chen, Polen Kocak, Eduardo Enciso-Martinez, Nicole Joy Bassous, Myung Chul Lee, Batzaya Byambaa, Zahra Rezaei, Yang Li, Maria Elizabeth Ubina Lopez, Melvin Gurian, Nebras Sobahi, Mohammad Asif Hussain, Laura Sanchez-Gonzalez, Jeroen Leijten, Shabir Hassan, Elmira Arab-Tehrany, Jennifer Ellis Ward, Su Ryon Shin
Summary: In this study, the inclusion of naturally occurring nanovesicles (EVs) into bioinks was explored to efficiently deliver gene expression regulators in bioprinted tissues. The fusion of EVs with liposomes was used to prolong and control their release, enabling efficient encapsulation and delivery of gene expression regulators.
Article
Chemistry, Multidisciplinary
Tom Kamperman, Niels G. A. Willemen, Cindy Kelder, Michelle Koerselman, Malin Becker, Luanda Lins, Castro Johnbosco, Marcel Karperien, Jeroen Leijten
Summary: This study demonstrates that stimuli-responsive cell-adhesive micromaterials can self-assemble with cells into 3D living composite microtissues and provide control over the biomechanical and biochemical environment, influencing cell behavior.
Article
Chemistry, Multidisciplinary
Malin Becker, Melvin Gurian, Maik Schot, Jeroen Leijten
Summary: Embedded 3D bioprinting using low-viscosity ink 3D (LoV3D) bioprinting based on aqueous two-phase stabilization allows for the fabrication of living constructs at high speeds with high viability. The liquid/liquid interfaces of LoV3D bioprinting offer unique advantages for fusing structures, creating vasculature, and modifying surfaces. The low interfacial tension of LoV3D bioprinting allows for nozzle-independent control over filament diameter, enabling the printing of a wide range of diameters down to the width of a single cell.
Article
Engineering, Biomedical
Maik Schot, Nuno Araujo-Gomes, Bas van Loo, Tom Kamperman, Jeroen Leijten
Summary: Living microtissues are increasingly being used due to their closer resemblance to native tissue physiology compared to 2D cultures. However, their application in industrial and clinical settings has been limited by the lack of scalability in production techniques. Continuous microfluidic processes offer a solution by providing higher throughput production rates and reproducible control over microtissue composition and size. This review provides an overview of current approaches to engineer microtissues, emphasizing the advantages and need for continuous processes to produce microtissues in large quantities.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Lea Andree, Pascal Bertsch, Rong Wang, Malin Becker, Jeroen Leijten, Peter Fischer, Fang Yang, Sander C. G. Leeuwenburgh
Summary: We developed a versatile hydrogel platform using modular building blocks, allowing for the design of customized hydrogels with specific physical architecture and mechanical properties. By assembling different combinations of gelatin methacryloyl (Gel-MA) and gelatin nanoparticles, we demonstrated the versatility of the platform. The incorporation of particles resulted in softer hydrogels with enhanced stress relaxation, and the stiffness of the hydrogels influenced cell behavior.
Article
Chemistry, Multidisciplinary
Shambhavi Pandey, Myungchul Lee, Jaewoon Lim, Sangbae Park, Yun-Hoon Choung, Jae Eun Kim, Pankaj Garg, Jong Hoon Chung
Summary: In this study, a therapy targeting cancer stem cells and non-stem cells in glioblastoma was developed by using CRISPR/Cas9 and siRNA to suppress key signaling pathways. The combined treatment effectively inhibited tumor growth and metastasis.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Deepti Rana, Prasanna Padmanaban, Malin Becker, Fabian Stein, Jeroen Leijten, Bart Koopman, Jeroen Rouwkema
Summary: Given the need for control over evolving biochemical cues within synthetic matrices, this study focused on the incorporation of VEGF165 specific aptamers into cell-instructive polymer networks to regulate VEGF bioactivity. Bicomponent micropatterns were fabricated via photopatterning and demonstrated the retention of aptamers, molecular recognition, and VEGF sequestration. The study also revealed the ability of aptamer-tethered VEGF to spatiotemporally regulate vascular morphogenesis.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Multidisciplinary
Vaishali Krishnadoss, Baishali Kanjilal, Arameh Masoumi, Aihik Banerjee, Iman Dehzangi, Arash Pezhouman, Reza Ardehali, Manuela Martins-Green, Jeroen Leijten, Iman Noshadi
Summary: The demand for personalized wearable and implantable medical devices has led to the development of electronics that interact with living systems. However, current bioelectronics still face challenges in establishing compatibility with biological systems. To address this, a gelatin-based bio-ink functionalized with a choline bio-ionic liquid (BIL) was developed for in situ 3D bioprinting of bioelectronics at the tissue interface. The resulting polymer is programmable, transparent, ion conductive, and flexible, making it suitable for personalized bioelectronics fabrication.
MATERIALS TODAY ADVANCES
(2023)
Article
Engineering, Biomedical
Shabir Hassan, Ting Wang, Kun Shi, Yike Huang, Maria Elizabeth Urbina Lopez, Kaifeng Gan, Mo Chen, Niels Willemen, Haroon Kalam, Eder Luna-Ceron, Berivan Cecen, Gihan Daw Elbait, Jinghang Li, Luis Enrique Garcia-Rivera, Melvin Gurian, Mudassir Meraj Banday, Kisuk Yang, Myung Chul Lee, Weida Zhuang, Castro Johnbosco, Oju Jeon, Eben Alsberg, Jeroen Leijten, Su Ryon Shin
Summary: Oxygenating biomaterials have been found to have beneficial effects on tissue formation, but the impact of oxygen-generating materials on tissue formation is not well understood. This study investigates the effects of calcium peroxide-based oxygen-generating microparticles on the osteogenic fate of human mesenchymal stem cells (hMSCs) under severely oxygen-deficient conditions. The results show that hydrogels containing oxygen-generating microparticles improve osteogenic differentiation of hMSCs under both normal and oxygen-deficient conditions. The study also demonstrates that hydrogels with oxygen-generating microparticles can induce the formation of functional engineered tissues, holding potential for biomedical applications such as tissue regeneration and organ replacement therapy.
Article
Engineering, Biomedical
Bas van Loo, Maik Schot, Melvin Gurian, Tom Kamperman, Jeroen Leijten
Summary: 3D cellular spheroids provide a more biomimetic microenvironment for tissue engineering applications compared to conventional 2D cell culture techniques. However, the clinical translation of spheroid tissue engineering is limited by current methods of spheroid formation. In this study, a clean, ultra-high throughput, single-step in-air microfluidic biofabrication technique for spheroid-forming compartmentalized hydrogels is reported. The technique allows for the production of hollow spheroid-forming compartments with different orientations and outperforms 2D cell cultures in terms of chondrogenic behavior.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Castro Johnbosco, Lisanne Karbaat, Nicoline M. Korthagen, Kelly Warmink, Michelle Koerselman, Katja Coeleveld, Malin Becker, Bas van Loo, Bram Zoetebier, Sanne Both, Harrie Weinans, Marcel Karperien, Jeroen Leijten
Summary: This study investigates the effect of micromaterial composition and concentration on the therapeutic outcome of intra-articular injections of microencapsulated stem cells for the treatment of osteoarthritis. The results demonstrate that different polymer compositions and concentrations can significantly alter the metabolic activity and secretome of MSCs, as well as the retention time and biodistribution within the joint. Furthermore, the study shows that the different microgel formulations have a material-dependent effect on reducing cartilage degradation and matrix loss in rat joints with osteoarthritis.
MATERIALS TODAY BIO
(2023)
Meeting Abstract
Orthopedics
K. Govindaraj, M. Meteling, M. Becker, J. Post, J. Leijten
OSTEOARTHRITIS AND CARTILAGE
(2022)