Article
Materials Science, Multidisciplinary
Riccardo Rizzo, Dominic Ruetsche, Hao Liu, Parth Chansoria, Anny Wang, Amelia Hasenauer, Marcy Zenobi-Wong
Summary: Multiscale printing of 3D perfusable geometries is achieved by combining volumetric printing (VP) and high-resolution two-photon ablation (2PA), overcoming the challenge of generating freeform designs with features ranging from centimeter to micrometer scales. Optical tuning of the photoresin's refractive index is used to eliminate micrometer-size defects generated during VP, allowing for defect-free printing that can be combined with 2PA. A protein-based photoclick photoresin is introduced to meet the requirements of the 2PA process and facilitate VP. This hybrid strategy enables the generation of complex organotypic 3D vasculature-like constructs with multiscale features.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Engineering, Biomedical
Margaret E. Prendergast, Jason A. Burdick
Summary: The study examines the characteristics of hydrogel inks in suspension bath printing through rheological, computational, and experimental analyses, emphasizing the importance of key rheological properties and print parameters in the printing process.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Biomedical
Joshua W. Tashman, Daniel J. Shiwarski, Brian Coffin, Alexander Ruesch, Frederick Lanni, Jana M. Kainerstorfer, Adam W. Feinberg
Summary: As 3D bioprinting continues to advance as a fabrication technology, there is a growing need for better analytical methods to characterize engineered constructs. This study presents a multimaterial bioprinting platform with integrated optical coherence tomography for in situ volumetric imaging, error detection, and 3D reconstruction. By improving the collagen bioink compositions, gelatin microparticle support bath optical clearing, and optimizing the machine pathing, the quality assessment of bioprinted constructs is enhanced.
Review
Chemistry, Multidisciplinary
Hossein Ravanbakhsh, Vahid Karamzadeh, Guangyu Bao, Luc Mongeau, David Juncker, Yu Shrike Zhang
Summary: Bioprinting aims at creating functional biomimetic constructs, and multi-material bioprinting allows for better replication of cellular diversity and tissue complexity, opening up new opportunities for tissue engineering, tissue model engineering, therapeutics development, and personalized medicine.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Gabriel Grossbacher, Michael Bartolf-Kopp, Csaba Gergely, Paulina Nunez Bernal, Sammy Florczak, Mylene de Ruijter, Nuria Gines Rodriguez, Juergen Groll, Jos Malda, Tomasz Jungst, Riccardo Levato
Summary: The major challenge in biofabrication is capturing the complex composition of native tissues. Current 3D printing techniques have limited capacity to produce composite biomaterials. Volumetric bioprinting, a light-based technique, allows for the fabrication of 3D structures with enhanced design freedom, but the prints have low mechanical stability.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Bram G. Soliman, Alessia Longoni, Mian Wang, Wanlu Li, Paulina N. Bernal, Alessandro Cianciosi, Gabriella C. J. Lindberg, Jos Malda, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jelena Rnjak-Kovacina, Tim B. F. Woodfield, Yu Shrike Zhang, Khoon S. Lim
Summary: Sacrificial printing involves using 3D-printed sacrificial ink to create open-channels in hydrogel constructs. Traditional sacrificial inks lack the ability to mimic tissue development dynamics. To overcome this, a new class of sacrificial inks was developed with tailorable and programmable delayed dissolution profiles. These inks showed compatibility with various biofabrication technologies and allowed precise control over the introduction of architectural features into cell-laden hydrogel constructs.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Polymer Science
Quinten Thijssen, Joseph Toombs, Chi Chung Li, Hayden Taylor, Sandra Van Vlieberghe
Summary: This review comprehensively discusses the potential of volumetric 3D-printing, summarizing the theoretical framework of photopolymerization and highlighting the advancements in light-based 3D-printing techniques. Special attention is given to the subfield of volumetric bioprinting. The review provides important discussions and prospects for those interested in the field of volumetric 3D-printing.
PROGRESS IN POLYMER SCIENCE
(2023)
Article
Engineering, Biomedical
Wei Long Ng, Xi Huang, Viktor Shkolnikov, Guo Liang Goh, Ratima Suntornnond, Wai Yee Yeong
Summary: A study utilizing a thermal inkjet system to dispense sub-nanoliter cell-laden droplets identified droplet impact velocity and droplet volume as significant factors affecting the viability and proliferation of printed cells. Increasing cell concentration leads to slower impact velocity, improving cell viability, while a minimum droplet volume of 20 nL helps mitigate evaporation-induced cell damage. Control of droplet impact velocity and volume is crucial for viability and proliferation of printed human primary cells in sub-nanoliter bioprinting.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Materials Science, Multidisciplinary
Marc Falandt, Paulina Nunez Bernal, Oksana Dudaryeva, Sammy Florczak, Gabriel Grossbacher, Matthias Schweiger, Alessia Longoni, Coralie Greant, Marisa Assuncao, Olaf Nijssen, Sandra van Vlierberghe, Jos Malda, Tina Vermonden, Riccardo Levato
Summary: This study presents a new approach using light-based volumetric printing to spatially pattern any biomolecule of interest on custom-designed hydrogels. By developing a gelatin norbornene resin with tunable mechanical properties, the resin can be quickly printed at high resolution. Thiol-ene click chemistry allows for on-demand photografting of thiolated compounds postprinting, enabling the precise biofunctionalization and modification of (bio)printed constructs to guide cell behavior and create bioactive cue gradients. This technology also opens possibilities for 4D printing to mimic the dynamic changes in morphogen presentation in biological tissues.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Computer Science, Artificial Intelligence
Xi Huang, Wei Long Ng, Wai Yee Yeong
Summary: In this study, a machine learning approach was used to predict the number of cells within printed droplets based on droplet velocity at two different points along the nozzle-substrate distance. A contactless method combining an optical system and machine learning algorithms was employed for cell detection within single droplets and prediction of the total number of printed cells within multiple droplets. Various machine learning algorithms were compared, and the random forest regressor algorithm achieved the highest accuracy in cell prediction within single droplets and the extra tree regressor algorithm demonstrated the lowest mean error in predicting the number of printed cells within multiple droplets. Incorporating these models in a droplet monitoring system enables live assessment of the number of printed cells during an inkjet-based bioprinting process.
JOURNAL OF INTELLIGENT MANUFACTURING
(2023)
Article
Chemistry, Multidisciplinary
Paulina Nunez Bernal, Manon Bouwmeester, Jorge Madrid-Wolff, Marc Falandt, Sammy Florczak, Nuria Gines Rodriguez, Yang Li, Gabriel Groessbacher, Roos-Anne Samsom, Monique van Wolferen, Luc J. W. van der Laan, Paul Delrot, Damien Loterie, Jos Malda, Christophe Moser, Bart Spee, Riccardo Levato
Summary: Organ- and tissue-level biological functions are closely related to microscale cell-cell interactions and tissue architecture. Biofabrication and organoid technologies have the potential to engineer multi-scale living constructs. This study introduces a volumetric bioprinting technique that captures key liver functions. The bioprinting process shapes organoid-laden gelatin hydrogels into complex 3D structures, allowing for rapid printing. Optically tuned bioresins and low stiffness gelatins enhance the viability and metabolism of the bioprinted constructs. This technology opens up new possibilities for regenerative medicine and personalized drug testing.
ADVANCED MATERIALS
(2022)
Article
Engineering, Biomedical
Daniel J. Shiwarski, Andrew R. Hudson, Joshua W. Tashman, Adam W. Feinberg
Summary: This Perspective discusses the challenges of 3D printing soft and liquid-like bioinks and the emergence of FRESH as a solution. The focus is on the customizability of the FRESH printing technique and its advantages in biofabrication. The future of FRESH printing is also considered, including both challenges and opportunities in the field of biofabrication.
APL BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Ke-Ning Chen, Bin-Guang Ma
Summary: The optoCRISPRi-HD system is a green-light activated CRISPRi system with a high dynamic range (40 fold) and the flexibility of changing targets. It can efficiently repress essential genes, nonessential genes, or inhibit the initiation of DNA replication. This study provides a regulative system with high resolution over space-time and extensive targets for further research involving complex gene networks, metabolic flux redirection, or bioprinting.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Engineering, Biomedical
Wei Long Ng, Teck Choon Ayi, Yi-Chun Liu, Swee Leong Sing, Wai Yee Yeong, Boon-Huan Tan
Summary: In this study, human triple-layered alveolar lung models were fabricated using the drop-on-demand (DOD) 3D bioprinting technique, which improved repeatability by achieving consistent cell output over time with different human alveolar cells. The 3D bioprinting platform offers an attractive tool for highly repeatable and scalable fabrication of 3D in-vitro human alveolar lung models.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Article
Engineering, Biomedical
Ada Frias-Sanchez, Diego A. Quevedo-Moreno, Mohamadmahdi Samandari, Jorge A. Tavares-Negrete, Victor Hugo Sanchez-Rodriguez, Ivonne Gonzalez-Gamboa, Fernando Ponz, Mario M. Alvarez, Grissel Trujillo-de Santiago
Summary: This study presents a method using chaotic flow to fabricate GelMA fibers, integrated with Turnip mosaic virus, for enhanced muscle tissue engineering. Experimental results demonstrate accurate fiber fabrication, improved cell attachment, and enhanced cell structure generation.
Review
Engineering, Biomedical
Arwa Daghrery, Isaac J. de Souza Araujo, Miguel Castilho, Jos Malda, Marco C. Bottino
Summary: For almost three decades, tissue engineering strategies have been used to create effective therapies for dental, oral, and craniofacial regenerative medicine by treating permanent deformities caused by debilitating health conditions. Additive manufacturing techniques, such as melt electrowriting (MEW), enable the creation of personalized scaffolds that can replicate native tissue characteristics using 3D printing technology. Recent advancements have shown that combining MEW with other biofabrication techniques can overcome some limitations and offer promising opportunities for tissue regeneration.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Leanne de Silva, Paulina N. Bernal, A. J. W. Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Summary: The development of tissue engineering strategies for treatment of large bone defects is crucial, and creating a vascular network is vital for tissue engineered bone constructs.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Arwa Daghrery, Jessica A. Ferreira, Jinping Xu, Nasim Golafshan, Darnell Kaigler, Sarit B. Bhaduri, Jos Malda, Miguel Castilho, Marco C. Bottino
Summary: This study engineered scaffolds with specific fiber morphology and structures to guide the differentiation of periodontal ligament stem cells and mediate macrophage polarization. The results showed that aligned fiber scaffolds can promote the formation of periodontal ligament, while fluorinated calcium phosphate-coated fibers with specific strand spacings can enhance bone tissue regeneration.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Gabriel Grossbacher, Michael Bartolf-Kopp, Csaba Gergely, Paulina Nunez Bernal, Sammy Florczak, Mylene de Ruijter, Nuria Gines Rodriguez, Juergen Groll, Jos Malda, Tomasz Jungst, Riccardo Levato
Summary: The major challenge in biofabrication is capturing the complex composition of native tissues. Current 3D printing techniques have limited capacity to produce composite biomaterials. Volumetric bioprinting, a light-based technique, allows for the fabrication of 3D structures with enhanced design freedom, but the prints have low mechanical stability.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Marc Falandt, Paulina Nunez Bernal, Oksana Dudaryeva, Sammy Florczak, Gabriel Grossbacher, Matthias Schweiger, Alessia Longoni, Coralie Greant, Marisa Assuncao, Olaf Nijssen, Sandra van Vlierberghe, Jos Malda, Tina Vermonden, Riccardo Levato
Summary: This study presents a new approach using light-based volumetric printing to spatially pattern any biomolecule of interest on custom-designed hydrogels. By developing a gelatin norbornene resin with tunable mechanical properties, the resin can be quickly printed at high resolution. Thiol-ene click chemistry allows for on-demand photografting of thiolated compounds postprinting, enabling the precise biofunctionalization and modification of (bio)printed constructs to guide cell behavior and create bioactive cue gradients. This technology also opens possibilities for 4D printing to mimic the dynamic changes in morphogen presentation in biological tissues.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Engineering, Biomedical
Jonathan H. Galarraga, Hannah M. Zlotnick, Ryan C. Locke, Sachin Gupta, Natalie L. Fogarty, Kendall M. Masada, Brendan D. Stoeckl, Lorielle Laforest, Miguel Castilho, Jos Malda, Riccardo Levato, James L. Carey, Robert L. Mauck, Jason A. Burdick
Summary: Soft norbornene-modified hyaluronic acid (NorHA) hydrogels were reinforced with polycaprolactone using melt electrowriting (MEW) to fabricate composite scaffolds. These scaffolds supported chondrogenesis and cartilage formation of encapsulated porcine mesenchymal stromal cells (pMSC) and exhibited suitable mechanical properties. Fixation of the composites with biodegradable pins or fibrin glue ensured implant retention, but the use of pins resulted in inferior cartilage repair compared to the use of glue.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Orthopedics
Jasper G. Steverink, Floris R. van Tol, Suzanne Bruins, Andre J. Smorenburg, Marianna A. Tryfonidou, Bas J. Oosterman, Marijke R. van Dijk, Jos Malda, Jorrit-Jan Verlaan
Summary: This study investigates the local toxic effects of highly concentrated (5%) bupivacaine compared to clinically used concentrations in vivo following skeletal surgery. The results show that bupivacaine concentration is negatively correlated with local tissue damage, and the mode of administration and implantation site have no significant effect on tissue damage.
JOURNAL OF EXPERIMENTAL ORTHOPAEDICS
(2023)
Article
Nanoscience & Nanotechnology
Abdel H. Mahmoud, Yuanyuan Han, Renan Dal-Fabbro, Arwa Daghrery, Jinping Xu, Darnell Kaigler, Sarit B. Bhaduri, Jos Malda, Marco C. Bottino
Summary: In this study, highly innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes were fabricated via electrospinning for guided bone regeneration (GBR). The composite microfibers showed a uniform porous network and successful integration of β-TCP particles. The GelMA/PCL-TCP membranes exhibited enhanced cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs) compared to pure PCL and GelMA/PCL. Furthermore, the GelMA/PCL-TCP membrane promoted robust bone regeneration in rat calvarial critical-size defects, demonstrating remarkable osteogenesis compared to PCL and GelMA/PCL groups. Overall, the electrospun GelMA/PCL-TCP composite showed great potential as a promising membrane for guided bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Anna Abbadessa, Paulina Nunez Bernal, Giorgio Buttitta, Alfredo Ronca, Ugo D'Amora, Carla Zihlmann, Niklaus Stiefel, Luigi Ambrosio, Jos Malda, Riccardo Levato, Jose Crecente-Campo, Maria Jose Alonso
Summary: Pathological angiogenesis plays a pivotal role in chronic diseases such as cancer, age-related macular degeneration, and osteoarthritis (OA). In this study, 3D printing was used to functionalize medical grade collagen with BVZ-loaded PLGA microparticles to target pathological angiogenesis. The encapsulation and release of BVZ were controlled by adjusting formulation parameters, and the anti-angiogenic activity of released BVZ was confirmed in a 3D cell model. Moreover, the collagen ink with embedded BVZ microparticles demonstrated excellent shape fidelity in 3D printed structures, such as meniscus, nose, and auricle-like structures.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Orthopedics
Andrea Schwab, Marinus A. Wesdorp, Jietao Xu, Florencia Abinzano, Claudia Loebel, Marc Falandt, Riccardo Levato, David Eglin, Roberto Narcisi, Martin J. Stoddart, Jos Malda, Jason A. Burdick, Matteo D'Este, Gerjo J. V. M. Van Osch
Summary: This study investigates the influence of various physicochemical properties of hydrogels on cell migration and related tissue formation using in vitro, ex vivo and in vivo models.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Multidisciplinary Sciences
Francesca Cialdai, Austin M. Brown, Cory W. Baumann, Debora Angeloni, Sarah Baatout, Alexandra Benchoua, Juergen Bereiter-Hahn, Daniele Bottai, Judith-Irina Buchheim, Marco Calvaruso, Eugenie Carnero-Diaz, Sara Castiglioni, Duccio Cavalieri, Gabriele Ceccarelli, Alexander Chouker, Gianni Ciofani, Giuseppe Coppola, Gabriella Cusella, Andrea Degl'Innocenti, Jean-Francois Desaphy, Jean-Pol Frippiat, Michael Gelinsky, Giada Genchi, Maria Grano, Daniela Grimm, Alain Guignandon, Christiane Hahn, Jason Hatton, Raul Herranz, Christine E. Hellweg, Carlo Saverio Iorio, Thodoris Karapantsios, Jack van Loon, Matteo Lulli, Jeanette Maier, Jos Malda, Emina Mamaca, Lucia Morbidelli, Angelique van Ombergen, Andreas Osterman, Aleksandr Ovsianikov, Francesco Pampaloni, Elizabeth Pavezlorie, Veronica Pereda-Campos, Cyrille Przybyla, Christopher Puhl, Petra Rettberg, Chiara Risaliti, Angela Maria Rizzo, Kate Robson-Brown, Leonardo Rossi, Giorgio Russo, Alessandra Salvetti, Daniela Santucci, Matthias Sperl, Felice Strollo, Kevin Tabury, Sara Tavella, Christiane Thielemann, Ronnie Willaert, Nathaniel J. Szewczyk, Monica Monici
Summary: This white paper presents the indications and recommendations of the SciSpacE Science Community on filling the gaps of knowledge regarding the effects of gravity alterations on animal and human systems at a cellular and tissue level. Despite previous studies, a comprehensive integrated model of the changes occurring at different system and functional levels is still lacking, making it difficult to predict the long-term consequences of human adaptation to the space environment and implement effective medical support plans.
Article
Biotechnology & Applied Microbiology
Mylene de Ruijter, Paweena Diloksumpan, Inge Dokter, Harold Brommer, Ineke H. Smit, Riccardo Levato, P. Rene van Weeren, Miguel Castilho, Jos Malda
Summary: A bioprinted osteochondral implant, based on gelatin methacryloyl cartilage phase and reinforced with precisely patterned melt electrowritten polycaprolactone fibers, can provide long-term mechanically stable neo-tissue in an animal model. Mechanical stability is more important than the presence of cells and pre-cultured extracellular matrix for the successful survival of the implant.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)