Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening

Active droplet sorting in microfluidics: a review

(2017) Heng-Dong Xi et al.   LAB ON A CHIP

Passive and active droplet generation with microfluidics: a review

(2017) Pingan Zhu et al.   LAB ON A CHIP

Hydrogel Droplet Microfluidics for High-Throughput Single Molecule/Cell Analysis

(2016) Zhi Zhu et al.   ACCOUNTS OF CHEMICAL RESEARCH

Dodecylresorufin (C12R) Outperforms Resorufin in Microdroplet Bacterial Assays

(2016) Ott Scheler et al.   ACS Applied Materials & Interfaces

Substrate Engineering Enabling Fluorescence Droplet Entrapment for IVC-FACS-Based Ultrahigh-Throughput Screening

(2016) Fuqiang Ma et al.   ANALYTICAL CHEMISTRY

hνSABR: Photochemical Dose–Response Bead Screening in Droplets

(2016) Alexander K. Price et al.   ANALYTICAL CHEMISTRY

Electrical lysis of cells for detergent-free droplet assays

(2016) N. de Lange et al.   Biomicrofluidics

High-throughput strategies for the discovery and engineering of enzymes for biocatalysis

(2016) Philippe Jacques et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Beyond the outer limits of nature by directed evolution

(2016) Patricia Molina-Espeja et al.   BIOTECHNOLOGY ADVANCES

Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants

(2016) Olaf Wagner et al.   LAB ON A CHIP

Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

(2016) Nachiket Shembekar et al.   LAB ON A CHIP

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase

(2016) Richard Obexer et al.   Nature Chemistry

iSpinach: a fluorogenic RNA aptamer optimized forin vitroapplications

(2016) Alexis Autour et al.   NUCLEIC ACIDS RESEARCH

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

(2016) Fabrice Gielen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Controlling molecular transport in minimal emulsions

(2016) Philipp Gruner et al.   Nature Communications

A general strategy for expanding polymerase function by droplet microfluidics

(2016) Andrew C. Larsen et al.   Nature Communications

Copper-free click chemistry for microdroplet's W/O interface engineering

(2016) S. Ursuegui et al.   RSC Advances

High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics

(2016) Thomas Beneyton et al.   Scientific Reports

Key Factors for Stable Retention of Fluorophores and Labeled Biomolecules in Droplet-Based Microfluidics

(2015) Jan-Willi Janiesch et al.   ANALYTICAL CHEMISTRY

Fluorinated Pickering Emulsions with Nonadsorbing Interfaces for Droplet-based Enzymatic Assays

(2015) Ming Pan et al.   ANALYTICAL CHEMISTRY

A Barcode-Free Combinatorial Screening Platform for Matrix Metalloproteinase Screening

(2015) Tushar D. Rane et al.   ANALYTICAL CHEMISTRY

Discovery in Droplets

(2015) Alexander K. Price et al.   ANALYTICAL CHEMISTRY

High-Throughput, Quantitative Enzyme Kinetic Analysis in Microdroplets Using Stroboscopic Epifluorescence Imaging

(2015) David Hess et al.   ANALYTICAL CHEMISTRY

Single-cell screening of photosynthetic growth and lactate production by cyanobacteria

(2015) Petter Hammar et al.   Biotechnology for Biofuels

Activity-Fed Translation (AFT) Assay: A New High-Throughput Screening Strategy for Enzymes in Droplets

(2015) Gabrielle Woronoff et al.   CHEMBIOCHEM

Directed Evolution of Enzymes for Industrial Biocatalysis

(2015) Joanne L. Porter et al.   CHEMBIOCHEM

Improving and repurposing biocatalysts via directed evolution

(2015) Carl A Denard et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Stabilisers for water-in-fluorinated-oil dispersions: Key properties for microfluidic applications

(2015) Philipp Gruner et al.   CURRENT OPINION IN COLLOID & INTERFACE SCIENCE

Enzyme engineering in biomimetic compartments

(2015) Pierre-Yves Colin et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Accurate microfluidic sorting of droplets at 30 kHz

(2015) Adam Sciambi et al.   LAB ON A CHIP

Dissecting enzyme function with microfluidic-based deep mutational scanning

(2015) Philip A. Romero et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

(2015) Mingtao Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions

(2015) Michael Ryckelynck et al.   RNA

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

(2015) Pierre-Yves Colin et al.   Nature Communications

Fluorinated Pickering Emulsions Impede Interfacial Transport and Form Rigid Interface for the Growth of Anchorage-Dependent Cells

(2014) Ming Pan et al.   ACS Applied Materials & Interfaces

CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics

(2014) Thomas Beneyton et al.   ANALYST

Droplet Electrospray Ionization Mass Spectrometry for High Throughput Screening for Enzyme Inhibitors

(2014) Shuwen Sun et al.   ANALYTICAL CHEMISTRY

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

(2014) Anastasia Zinchenko et al.   ANALYTICAL CHEMISTRY

A high-throughput cellulase screening system based on droplet microfluidics

(2014) Raluca Ostafe et al.   Biomicrofluidics

Droplet-Based Microfluidics Platform for Ultra-High-Throughput Bioprospecting of Cellulolytic Microorganisms

(2014) Majdi Najah et al.   CHEMISTRY & BIOLOGY

Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase

(2014) Raluca Ostafe et al.   CHEMISTRY & BIOLOGY

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

(2014) Han Xiao et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Enzyme Promiscuity: Engine of Evolutionary Innovation

(2014) Chetanya Pandya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

(2014) Lothar Schmid et al.   LAB ON A CHIP

Generating electric fields in PDMS microfluidic devices with salt water electrodes

(2014) Adam Sciambi et al.   LAB ON A CHIP

Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems

(2014) Minsoung Rhee et al.   LAB ON A CHIP

Sugar Additives Improve Signal Fidelity for Implementing Two-Phase Resorufin-Based Enzyme Immunoassays

(2014) Patrick A. Sandoz et al.   LANGMUIR

Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption

(2014) Benjamin L Wang et al.   NATURE BIOTECHNOLOGY

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

An Improved Single Cell Ultrahigh Throughput Screening Method Based on In Vitro Compartmentalization

(2014) Fuqiang Ma et al.   PLoS One

One drop at a time: toward droplet microfluidics as a versatile tool for single-cell analysis

(2014) Agata Rakszewska et al.   NPG Asia Materials

Analytical detection techniques for droplet microfluidics—A review

(2013) Ying Zhu et al.   ANALYTICA CHIMICA ACTA

A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition

(2013) Fabrice Gielen et al.   ANALYTICAL CHEMISTRY

New Glycosidase Substrates for Droplet-Based Microfluidic Screening

(2013) Majdi Najah et al.   ANALYTICAL CHEMISTRY

Creating Biocompatible Oil–Water Interfaces without Synthesis: Direct Interactions between Primary Amines and Carboxylated Perfluorocarbon Surfactants

(2013) Cheryl J. DeJournette et al.   ANALYTICAL CHEMISTRY

Synthesis of Nanostructured and Biofunctionalized Water-in-Oil Droplets as Tools for Homing T Cells

(2013) Ilia Platzman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

High-throughput screening for industrial enzyme production hosts by droplet microfluidics

(2013) Staffan L. Sjostrom et al.   LAB ON A CHIP

Automated analysis of dynamic behavior of single cells in picoliter droplets

(2013) Mohammad Ali Khorshidi et al.   LAB ON A CHIP

Engineering microfluidic concentration gradient generators for biological applications

(2013) Alicia G. G. Toh et al.   Microfluidics and Nanofluidics

Droplet microfluidics driven by gradients of confinement

(2013) R. Dangla et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Flow cytometry-based ultra-high-throughput screening assay for cellulase activity

(2012) Raluca Ostafe et al.   ANALYTICAL BIOCHEMISTRY

Biocatalyst development by directed evolution

(2012) Meng Wang et al.   BIORESOURCE TECHNOLOGY

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

(2012) Balint Kintses et al.   CHEMISTRY & BIOLOGY

Directed evolution: tailoring biocatalysts for industrial applications

(2012) Ashwani Kumar et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Directed enzyme evolution: beyond the low-hanging fruit

(2012) Moshe Goldsmith et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets

(2012) Miriam Kaltenbach et al.   LAB ON A CHIP

Characterization of sensitivity and specificity in leaky droplet-based assays

(2012) Yunhan Chen et al.   LAB ON A CHIP

Selective droplet coalescence using microfluidic systems

(2012) Linas Mazutis et al.   LAB ON A CHIP

Droplet microfluidics for high-throughput biological assays

(2012) Mira T. Guo et al.   LAB ON A CHIP

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

(2012) Ali Fallah-Araghi et al.   LAB ON A CHIP

Functional single-cell hybridoma screening using droplet-based microfluidics

(2012) B. E. Debs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamics of molecular transport by surfactants in emulsions

(2012) Yousr Skhiri et al.   Soft Matter

Creation of Stepwise Concentration Gradient in Picoliter Droplets for Parallel Reactions of Matrix Metalloproteinase II and IX

(2011) Sachin Jambovane et al.   ANALYTICAL CHEMISTRY

Enzyme Kinetic Measurements Using a Droplet-Based Microfluidic System with a Concentration Gradient

(2011) Minh-Phuong Ngoc Bui et al.   ANALYTICAL CHEMISTRY

Droplet-Based Microfluidic Flow Injection System with Large-Scale Concentration Gradient by a Single Nanoliter-Scale Injection for Enzyme Inhibition Assay

(2011) Long-Fei Cai et al.   ANALYTICAL CHEMISTRY

New Generation of Amino Coumarin Methyl Sulfonate-Based Fluorogenic Substrates for Amidase Assays in Droplet-Based Microfluidic Applications

(2011) Gabrielle Woronoff et al.   ANALYTICAL CHEMISTRY

Teaching Single-Cell Digital Analysis Using Droplet-Based Microfluidics

(2011) Majdi Najah et al.   ANALYTICAL CHEMISTRY

Self-Regulated, Droplet-Based Sample Chopper for Microfluidic Absorbance Detection

(2011) Kennon S. Deal et al.   ANALYTICAL CHEMISTRY

The great screen anomaly—a new frontier in product discovery through functional metagenomics

(2011) David Matthias Ekkers et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

On-demand electrostatic droplet charging and sorting

(2011) Byungwook Ahn et al.   Biomicrofluidics

Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator

(2011) Chia-Hung Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Quantitative and sensitive detection of rare mutations using droplet-based microfluidics

(2011) Deniz Pekin et al.   LAB ON A CHIP

Surfactants in droplet-based microfluidics

(2011) Jean-Christophe Baret   LAB ON A CHIP

Directed evolution of hydrolases for prevention of G-type nerve agent intoxication

(2011) Rinkoo D Gupta et al.   Nature Chemical Biology

Ribozyme-Catalyzed Transcription of an Active Ribozyme

(2011) A. Wochner et al.   SCIENCE

Generation of Picoliter Droplets with Defined Contents and Concentration Gradients from the Separation of Chemical Mixtures

(2010) Ashleigh B. Theberge et al.   ANALYTICAL CHEMISTRY

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

Microfluidic sorting with high-speed single-layer membrane valves

(2010) Adam R. Abate et al.   APPLIED PHYSICS LETTERS

Advanced microfluidic droplet manipulation based on piezoelectric actuation

(2010) Jonathan Shemesh et al.   BIOMEDICAL MICRODEVICES

Strategies for culture of ‘unculturable’ bacteria

(2010) Sonia R. Vartoukian et al.   FEMS MICROBIOLOGY LETTERS

Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

(2010) Jian Pei et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

High-throughput sample introduction for droplet-based screening with an on-chip integrated sampling probe and slotted-vial array

(2010) Meng Sun et al.   LAB ON A CHIP

Dynamics of microfluidic droplets

(2010) Charles N. Baroud et al.   LAB ON A CHIP

High-throughput injection with microfluidics using picoinjectors

(2010) A. R. Abate et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays

(2009) Fabienne Courtois et al.   ANALYTICAL CHEMISTRY

Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis

(2009) Linas Mazutis et al.   ANALYTICAL CHEMISTRY

Directed Evolution of a Thermophilic β-glucosidase for Cellulosic Bioethanol Production

(2009) Elizabeth Hardiman et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

Novel trends in high-throughput screening

(2009) Lorenz M Mayr et al.   CURRENT OPINION IN PHARMACOLOGY

Laterally Mobile, Functionalized Self-Assembled Monolayers at the Fluorous−Aqueous Interface in a Plug-Based Microfluidic System: Characterization and Testing with Membrane Protein Crystallization

(2009) Jason E. Kreutz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices

(2009) Thomas Franke et al.   LAB ON A CHIP

Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme

(2009) Linas Mazutis et al.   LAB ON A CHIP

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Monodisperse Colloids Synthesized with Nanofluidic Technology

(2009) Florent Malloggi et al.   LANGMUIR

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Impact of inlet channel geometry on microfluidic drop formation

(2009) A. R. Abate et al.   PHYSICAL REVIEW E

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets

(2008) Margaret Macris Kiss et al.   ANALYTICAL CHEMISTRY

Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

(2008) Yolanda Schaerli et al.   ANALYTICAL CHEMISTRY

From Microdroplets to Microfluidics: Selective Emulsion Separation in Microfluidic Devices

(2008) Luis M. Fidalgo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Droplet-Based Microreactors for the Synthesis of Magnetic Iron Oxide Nanoparticles

(2008) Lucas Frenz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Integrated Device for Monitoring Time-Dependent in vitro Expression From Single Genes in Picolitre Droplets

(2008) Fabienne Courtois et al.   CHEMBIOCHEM

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

(2008) Jenifer Clausell-Tormos et al.   CHEMISTRY & BIOLOGY

Pillar-induced droplet merging in microfluidic circuits

(2008) Xize Niu et al.   LAB ON A CHIP

Biocompatible surfactants for water-in-fluorocarbon emulsions

(2008) C. Holtze et al.   LAB ON A CHIP

Reliable microfluidic on-chip incubation of droplets in delay-lines

(2008) Lucas Frenz et al.   LAB ON A CHIP

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

(2008) Ansgar Huebner et al.   LAB ON A CHIP

Decompressing Emulsion Droplets Favors Coalescence

(2008) Nicolas Bremond et al.   PHYSICAL REVIEW LETTERS

Drop Mixing in a Microchannel for Lab-on-a-Chip Platforms

(2007) Minsoung Rhee et al.   LANGMUIR