Quantitative and sensitive detection of rare mutations using droplet-based microfluidics

Reply to D. Santini et al

(2011) Leonor Benhaim et al.   JOURNAL OF CLINICAL ONCOLOGY

Should Oncologists Be Aware in Their Clinical Practice ofKRASMolecular Analysis?

(2011) Daniele Santini et al.   JOURNAL OF CLINICAL ONCOLOGY

Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR

(2011) Qun Zhong et al.   LAB ON A CHIP

Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology

(2010) Ashleigh B. Theberge et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Circulating free tumor DNA and colorectal cancer

(2010) T. Lecomte et al.   GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE

A Commercial Real-Time PCR Kit Provides Greater Sensitivity than Direct Sequencing to Detect KRAS Mutations

(2010) Bárbara Angulo et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Comparison of Sanger Sequencing, Pyrosequencing, and Melting Curve Analysis for the Detection of KRAS Mutations

(2010) Athanasios C. Tsiatis et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Digital PCR on a SlipChip

(2010) Feng Shen et al.   LAB ON A CHIP

High-throughput fluorescence detection using an integrated zone-plate array

(2010) Ethan Schonbrun et al.   LAB ON A CHIP

Detection ofKRASmutations in colorectal carcinoma patients with an integrated PCR/sequencing and real-time PCR approach

(2010) Pietro Carotenuto et al.   PHARMACOGENOMICS

Pyrosequencing method to detect KRAS mutation in formalin-fixed and paraffin-embedded tumor tissues

(2009) Sandrine Dufort et al.   ANALYTICAL BIOCHEMISTRY

Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis

(2009) Linas Mazutis et al.   ANALYTICAL CHEMISTRY

COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing

(2009) Jin Li et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Performance of nanoliter-sized droplet-based microfluidic PCR

(2009) Fang Wang et al.   BIOMEDICAL MICRODEVICES

Single-Molecule Detection of Epidermal Growth Factor Receptor Mutations in Plasma by Microfluidics Digital PCR in Non-Small Cell Lung Cancer Patients

(2009) T. K.F. Yung et al.   CLINICAL CANCER RESEARCH

PCR-Based Methods for the Enrichment of Minority Alleles and Mutations

(2009) C. A. Milbury et al.   CLINICAL CHEMISTRY

Beyond the KRAS test

(2009) Pierre Laurent-Puig et al.   EUROPEAN JOURNAL OF CANCER

A fast and efficient microfluidic system for highly selective one-to-one droplet fusion

(2009) Linas Mazutis et al.   LAB ON A CHIP

Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme

(2009) Linas Mazutis et al.   LAB ON A CHIP

The cancer genome

(2009) Michael R. Stratton et al.   NATURE

Microdroplet-based PCR enrichment for large-scale targeted sequencing

(2009) Ryan Tewhey et al.   NATURE BIOTECHNOLOGY

Sensitive digital quantification of DNA methylation in clinical samples

(2009) Meng Li et al.   NATURE BIOTECHNOLOGY

Predictive value of KRAS mutations in chemoresistant CRC

(2009) Astrid Lièvre et al.   Nature Reviews Clinical Oncology

Alterations in Genes of the EGFR Signaling Pathway and Their Relationship to EGFR Tyrosine Kinase Inhibitor Sensitivity in Lung Cancer Cell Lines

(2009) Jeet Gandhi et al.   PLoS One

Oncogene Mutations, Copy Number Gains and Mutant Allele Specific Imbalance (MASI) Frequently Occur Together in Tumor Cells

(2009) Junichi Soh et al.   PLoS One

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets

(2008) N. Reginald Beer et al.   ANALYTICAL CHEMISTRY

High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets

(2008) Palani Kumaresan et al.   ANALYTICAL CHEMISTRY

High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets

(2008) Margaret Macris Kiss et al.   ANALYTICAL CHEMISTRY

Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

(2008) Yolanda Schaerli et al.   ANALYTICAL CHEMISTRY

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

(2008) Jenifer Clausell-Tormos et al.   CHEMISTRY & BIOLOGY

Analysis of Mutations in DNA Isolated From Plasma and Stool of Colorectal Cancer Patients

(2008) Frank Diehl et al.   GASTROENTEROLOGY

KRASMutations As an Independent Prognostic Factor in Patients With Advanced Colorectal Cancer Treated With Cetuximab

(2008) Astrid Lièvre et al.   JOURNAL OF CLINICAL ONCOLOGY

Biocompatible surfactants for water-in-fluorocarbon emulsions

(2008) C. Holtze et al.   LAB ON A CHIP

Absolute quantitative detection of ABL tyrosine kinase domain point mutations in chronic myeloid leukemia using a novel nanofluidic platform and mutation-specific PCR

(2008) V G Oehler et al.   LEUKEMIA

The cancer biomarker problem

(2008) Charles L. Sawyers   NATURE

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing

(2008) Jin Li et al.   NATURE MEDICINE

Studying copy number variations using a nanofluidic platform

(2008) Jian Qin et al.   NUCLEIC ACIDS RESEARCH

High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array

(2008) Sandra L. Spurgeon et al.   PLoS One

Digital PCR: a powerful new tool for noninvasive prenatal diagnosis?

(2008) Bernhard G. Zimmermann et al.   PRENATAL DIAGNOSIS