Directed evolution: tailoring biocatalysts for industrial applications

Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase

(2011) Eva Garcia-Ruiz et al.   BIOCHEMICAL JOURNAL

Directed evolution of a pyruvate aldolase to recognize a long chain acyl substrate

(2011) Manoj Cheriyan et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Strategy and success for the directed evolution of enzymes

(2011) Paul A Dalby   CURRENT OPINION IN STRUCTURAL BIOLOGY

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries

(2011) Burckhard Seelig   Nature Protocols

Recent progress in industrial biocatalysis

(2010) Bettina M Nestl et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Protein engineering of microbial enzymes

(2010) Dominique Böttcher et al.   CURRENT OPINION IN MICROBIOLOGY

A Semi-Rational Approach to Engineering Laccase Enzymes

(2010) Annalisa Miele et al.   MOLECULAR BIOTECHNOLOGY

Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination

(2010) Pete Heinzelman et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Converting an Esterase into an Epoxide Hydrolase

(2009) Helge Jochens et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Construction and engineering of a thermostable self-sufficient cytochrome P450

(2009) Takao Mandai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Directed evolution: new parts and optimized function

(2009) Michael J Dougherty et al.   CURRENT OPINION IN BIOTECHNOLOGY

Directed enzyme evolution: climbing fitness peaks one amino acid at a time

(2009) Cara A Tracewell et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Error-prone PCR of a fungal xylanase for improvement of its alkaline and thermal stability

(2009) Dawn Elizabeth Stephens et al.   FEMS MICROBIOLOGY LETTERS

A green-by-design biocatalytic process for atorvastatin intermediate

(2009) Steven K. Ma et al.   GREEN CHEMISTRY

SCHEMA Recombination of a Fungal Cellulase Uncovers a Single Mutation That Contributes Markedly to Stability

(2009) Pete Heinzelman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Expression of an alkalo-tolerant fungal xylanase enhanced by directed evolution in Pichia pastoris and Escherichia coli

(2009) Nokuthula Peace Mchunu et al.   JOURNAL OF BIOTECHNOLOGY

Starch and α-glucan acting enzymes, modulating their properties by directed evolution

(2009) Ronan M. Kelly et al.   JOURNAL OF BIOTECHNOLOGY

Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series

(2009) Olga Khersonsky et al.   JOURNAL OF MOLECULAR BIOLOGY

Technical methods to improve yield, activity and stability in the development of microbial lipases

(2009) Zheng-Yu Shu et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Highly Enantioselective Reduction of a Small Heterocyclic Ketone: Biocatalytic Reduction of Tetrahydrothiophene-3-one to the Corresponding (R)-Alcohol

(2009) Jack Liang et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A family of thermostable fungal cellulases created by structure-guided recombination

(2009) P. Heinzelman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improving low-temperature activity ofSulfolobus acidocaldarius2-keto-3-deoxygluconate aldolase

(2009) Suzanne Wolterink-van Loo et al.   ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL

Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension

(2008) Raj Kumar et al.   ANALYTICAL BIOCHEMISTRY

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Improving the thermostability of N-carbamyl-d-amino acid amidohydrolase by error-prone PCR

(2008) Hong Yu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed evolution of a Baeyer–Villiger monooxygenase to enhance enantioselectivity

(2008) Anett Kirschner et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution

(2008) Ronan M. Kelly et al.   BIOCHEMICAL JOURNAL

Laboratory evolution of Pyrococcus furiosus alcohol dehydrogenase to improve the production of (2S,5S)-hexanediol at moderate temperatures

(2008) Ronnie Machielsen et al.   EXTREMOPHILES

Alteration of coenzyme specificity in halophilic NAD(P)+ glucose dehydrogenase by site-directed mutagenesis

(2008) C. Pire et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution

(2008) Aurelio Hidalgo et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Combinatorial engineering to enhance thermostability of amylosucrase

(2008) Stéphane Emond et al.   PROTEIN SCIENCE

Directed evolution of a bacterial α-amylase: Toward enhanced pH-performance and higher specific activity

(2003) Cornelius Bessler et al.   PROTEIN SCIENCE