Article
Biochemistry & Molecular Biology
Nancy B. Andrioli, Mariela Nieves, Mariel Poltronieri, Chiara Bonzon, Gabriela Chaufan
Summary: In this study, the genotoxicity response of HEp-2 cell line to sub-cytotoxic concentrations of tebuconazole (TB) was evaluated. It was found that TB induced negative centromere micronuclei (MNC-) and DNA damage, suggesting a clastogenic effect. Furthermore, the comet assay results showed that DNA damage in the HEp-2 cell line could be repaired in the presence of low TB concentrations. Therefore, the safety of applying sub-cytotoxic concentrations of TB should be assessed.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Review
Cell Biology
Davide Cinat, Robert P. Coppes, Lara Barazzuol
Summary: This review discusses how DNA damage affects the maintenance and function of stem cells in adult tissues and neoplasms, leading to inflammatory responses and changes in the microenvironment. DNA damage can induce cell senescence, increase pro-inflammatory factors release, and modulate immune responses, potentially impacting tissue homeostasis and cancer development. Understanding the DNA damage-induced immunomodulatory responses on stem cell microenvironment may provide insights into age-related diseases and cancer, offering novel treatment strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Eliana M. Barros, Stuart A. McIntosh, Kienan I. Savage
Summary: Genomic instability is a hallmark of tumourigenesis, and defects in the DNA damage response (DDR) play a crucial role in shaping therapeutic response. The cGAS/STING signalling pathway has been identified as a key mediator of inflammation in response to DNA damage. Multiple DNA repair defects and DNA damage induced by conventional chemotherapy/radiation can also activate the innate immune response through cGAS/STING activation. Combining DNA damaging agents with immunotherapies has potential therapeutic opportunities.
Review
Biochemistry & Molecular Biology
Jolanta Kwasniewska, Adrianna Wiktoria Bara
Summary: Cytogenetic approaches are important for evaluating the genetic effects of mutagenic treatments, and although they are time-consuming, they are essential for studying cytotoxic and genotoxic effects and environmental monitoring. The micronucleus test is an important method for studying genotoxicity in plants, and recent advancements have expanded the scope of cytogenetic techniques beyond the detection of chromosome aberrations. However, there is limited knowledge about micronuclei in plants compared to humans and animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Fany Renata Aguilera-Rodriguez, Ana Lourdes Zamora-Perez, Clara Luz Galvan-Moreno, Rosalinda Gutierrez-Hernandez, Claudia Araceli Reyes Estrada, Edgar L. Esparza-Ibarra, Blanca Patricia Lazalde-Ramos
Summary: The cytotoxicity and genotoxicity of aqueous and hydroalcoholic leaf and bark extracts of Crataegus oxyacantha were evaluated using the micronucleus test in a murine model. Results showed genotoxic effects for both leaf and bark extracts, while only the aqueous leaf extract exhibited cytotoxic effects.
Article
Biology
He Ren, Chaobing Ma, Haoran Peng, Bo Zhang, Lulin Zhou, Yan Su, Xiaoyan Gao, Hongyan Huang
Summary: Research showed that SARS-CoV-2 infection could lead to the formation of micronuclei within cells, activating DNA damage response and cGAS-STING signaling, providing insights into the pathological effects of the infection and potential targets for COVID-19 therapy.
Review
Oncology
Nura Lutfi, Miguel Alejandro Galindo-Campos, Jose Yelamos
Summary: Targeting tumour-specific DDR defects for new therapeutic approaches may impact the crosstalk between tumour cells and the immune system, potentially influencing the antitumour immune response and providing new clues for developing cancer therapies.
Review
Cell Biology
Huizhi Lu, Yuejin Hua
Summary: DDR pathways are crucial for maintaining genome integrity against various damaging events. The newly identified PprI-DdrO system in Deinococcus plays a key role in DNA damage response, with PprI mediating extreme resistance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Thomas J. Hayman, Peter M. Glazer
Summary: Research on disease treatment has focused on DNA repair pathways, with potential for enhanced efficacy with novel immunotherapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Tamara Cetkovic Pecar, Anja Haveric, Lejla Caluk Klacar, Sanin Haveric, Alen Dzaferspahic, Mahira Mehanovic, Irma Durmisevic, Selma Dzaferspahic, Maida Hadzic Omanovic
Summary: This study found that DNA damage in oral leukocytes and buccal cells was more severe in young waterpipe smokers from Bosnia and Herzegovina compared to non-smokers.
Article
Nanoscience & Nanotechnology
Tao Wu, Shengmin Xu, Biao Chen, Lingzhi Bao, Jie Ma, Wei Han, An Xu, Kwan Ngok Yu, Lijun Wu, Shaopeng Chen
Summary: This study demonstrates that PM2.5 exposure aggravates cellular senescence and reveals the crucial role of micronuclei. The results indicate that the nuclear envelope of PM2.5-induced micronuclei is ruptured, leading to the activation of the cGAS-STING pathway, which is associated with cellular senescence.
Article
Chemistry, Medicinal
Zhongren Xu, Qiuyue Lu, Min Shan, Guizhi Jiang, Yuanhao Liu, Zhibin Yang, Yunlong Lu, Wukun Liu
Summary: Inflammation promotes ovarian cancer development, and chemoresistance is a major challenge in its treatment. A series of gold(I) complexes derived from NSAIDs were synthesized, and complex B3 (Npx-Au) exhibited higher antitumor activity than cisplatin and other gold(I) complexes. Npx-Au induced oxidative stress and the DAMPs process by inhibiting TrxR activity. Mechanistic studies showed simultaneous downregulation of COX-2 and PD-L1 after Npx-Au treatment. Interestingly, in vivo experiments demonstrated that Npx-Au treatment stimulated immune response by reducing PD-L1 expression, inducing DC maturation, and increasing T cell infiltration. Overall, Npx-Au elicited immunogenic cell death and showed promise as a strategy for chemotherapy combined with immunotherapy in treating ovarian cancer.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Environmental Sciences
Yan Jin, Yiting Li, Shiyi He, Yijun Ge, Yun Zhao, Ke Zhu, Andong He, Siyu Li, Siyu Yan, Chao Cao
Summary: PM2.5 induces airway inflammation and DNA damage, and the ATM gene plays a role in promoting this inflammation.
ENVIRONMENTAL TOXICOLOGY
(2023)
Review
Oncology
Congqi Shi, Kaiyu Qin, Anqi Lin, Aimin Jiang, Quan Cheng, Zaoqu Liu, Jian Zhang, Peng Luo
Summary: This study summarizes currently identified and promising biomarkers for predicting the response of oncology patients to immune checkpoint inhibitors, and explores the mechanism of combination therapy with immune checkpoint inhibitors and DNA damage repair inhibitors.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Review
Immunology
Wen-shan Li, Qing-qing Zhang, Qiao Li, Shang-yu Liu, Guo-qiang Yuan, Ya-wen Pan
Summary: The imbalance of immune response is crucial in disease development, including glioblastoma. Understanding how the innate immune system detects tumors and pathogens is essential. This review emphasizes the similarity in RNA and DNA sensing mechanisms in the innate immunity of both tumors and pathogens, with the aim of enhancing the anti-tumor innate immune response and exploring new targets to improve the response to conventional tumor therapy and immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Saskia F. Erttmann, Anetta Hartlova, Marta Sloniecka, Faizal A. M. Raffi, Ava Hosseinzadeh, Tomas Edgren, Reza Rofougaran, Ulrike Resch, Maria Fallman, Torben Ek, Nelson O. Gekara
Article
Immunology
Chaitanya Kurhade, Loreen Zegenhagen, Elvira Weber, Sharmila Nair, Kristin Michaelsen-Preusse, Julia Spanier, Nelson O. Gekara, Andrea Kroeger, Anna K. Overby
JOURNAL OF NEUROINFLAMMATION
(2016)
Article
Immunology
Richard Lindqvist, Filip Mundt, Jonathan D. Gilthorpe, Silke Woelfel, Nelson O. Gekara, Andrea Kroeger, Anna K. Overby
JOURNAL OF NEUROINFLAMMATION
(2016)
Correction
Immunology
Saskia F. Erttmann, Anetta Hartlova, Marta Sloniecka, Faizal A. M. Raffi, Ava Hosseinzadeh, Tomas Edgren, Reza Rofougaran, Ulrike Resch, Maria Fallman, Torben Ek, Nelson O. Gekara
Article
Microbiology
Steve J. Webster, Sven Brode, Lou Ellis, Timothy J. Fitzmaurice, Matthew J. Elder, Nelson O. Gekara, Panagiotis Tourlomousis, Clare Bryant, Simon Clare, Ronnie Chee, Hill J. S. Gaston, Jane C. Goodall
Article
Multidisciplinary Sciences
Swarupa Panda, Nelson O. Gekara
NATURE COMMUNICATIONS
(2018)
Editorial Material
Biochemistry & Molecular Biology
Nelson O. Gekara, Hui Jiang
Article
Biochemistry & Molecular Biology
Hui Jiang, Xiaoyu Xue, Swarupa Panda, Ajinkya Kawale, Richard M. Hooy, Fengshan Liang, Jungsan Sohn, Patrick Sung, Nelson O. Gekara
Article
Multidisciplinary Sciences
Saskia F. Erttmann, Nelson O. Gekara
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Pilar Samperio Ventayol, Petra Geiser, Maria Letizia Di Martino, Alexandra Florbrant, Stefan A. Fattinger, Naemi Walder, Eduardo Sima, Feng Shao, Nelson O. Gekara, Magnus Sundbom, Wolf-Dietrich Hardt, Dominic-Luc Webb, Per M. Hellstrom, Jens Eriksson, Mikael E. Sellin
Summary: The study found that infection with Salmonella Typhimurium triggers focal contractions in intestinal epithelial cells, which are initiated by the NAIP/NLRC4 inflammasome and can prevent infected cell death and expulsion, thereby protecting the integrity of the intestinal epithelium.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Hui Jiang, Patrycja Swacha, Nelson O. Gekara
Summary: Mice lacking ALRs are resistant to radiation-induced BM injury, as nuclear ALRs act as inhibitors of DNA repair, accelerating genome destabilization, micronuclei generation, and cell death. The study uncovers ALRs as potential targets for interventions against genotoxic tissue injury and as potential biomarkers for predicting the outcome of radio/chemotherapy.
Article
Biochemical Research Methods
Saskia F. Erttmann, Nelson O. Gekara
Summary: This article describes a protocol for enriching bacterial membrane vesicles from serum and colon, which can be adapted for other tissues. The protocol involves pre-clearing serum or colon fluids using 0.2-mm syringe filters, concentrating the vesicles using centrifugal filter devices, isolating the vesicles with qEV size exclusion columns, and concentrating the isolated vesicle fractions for downstream analyses. For more detailed information on the use and execution of this protocol, please refer to Erttmann et al. (2022).
Letter
Cell Biology
Hui Jiang, Patrycja Swacha, Kyaw Min Aung, Nelson O. Gekara
Article
Cell Biology
Daniela Putzova, Swarupa Panda, Anetta Hartlova, Jiri Stulik, Nelson O. Gekara
CELLULAR MICROBIOLOGY
(2017)
