Coupled catalytic states and the role of metal coordination in Cas9

Structural principles of CRISPR-Cas enzymes used in nucleic acid detection

(2022) Anuska Das et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structural basis for mismatch surveillance by CRISPR–Cas9

(2022) Jack P. K. Bravo et al.   NATURE

R-loop formation and conformational activation mechanisms of Cas9

(2022) Martin Pacesa et al.   NATURE

Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR–Cas9

(2022) Łukasz Nierzwicki et al.   Nature Catalysis

Catalytically Enhanced Cas9 Through Directed Protein Evolution

(2021) Travis H. Hand et al.   CRISPR Journal

New tools for automated cryo-EM single-particle analysis in RELION-4.0

(2021) Dari Kimanius et al.   BIOCHEMICAL JOURNAL

Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2

(2020) Xue Huang et al.   Nature Communications

The molecular basis for recognition of 5′-NNNCC-3′ PAM and its methylation state by Acidothermus cellulolyticus Cas9

(2020) Anuska Das et al.   Nature Communications

Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9

(2019) Xing Zhu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix

(2019) Dorothee Liebschner et al.   Acta Crystallographica Section D-Structural Biology

Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States

(2019) Wei Sun et al.   MOLECULAR CELL

Functional Insights Revealed by the Kinetic Mechanism of CRISPR/Cas9

(2018) Austin T. Raper et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

DNA Unwinding Is the Primary Determinant of CRISPR-Cas9 Activity

(2018) Shanzhong Gong et al.   Cell Reports

Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing

(2018) Daan C. Swarts et al.   Wiley Interdisciplinary Reviews-RNA

Phosphate Lock Residues of Acidothermus cellulolyticus Cas9 Are Critical to Its Substrate Specificity

(2018) Travis H. Hand et al.   ACS Synthetic Biology

Time-resolved cryo-electron microscopy: Recent progress

(2017) Joachim Frank   JOURNAL OF STRUCTURAL BIOLOGY

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

The Impact of DNA Topology and Guide Length on Target Selection by a Cytosine-Specific Cas9

(2017) Tsz Kin Martin Tsui et al.   ACS Synthetic Biology

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9

(2017) Yavuz S. Dagdas et al.   Science Advances

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes

(2015) Enbo Ma et al.   MOLECULAR CELL

Expanding the Biologist’s Toolkit with CRISPR-Cas9

(2015) Samuel H. Sternberg et al.   MOLECULAR CELL

Conformational control of DNA target cleavage by CRISPR–Cas9

(2015) Samuel H. Sternberg et al.   NATURE

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Nucleases: diversity of structure, function and mechanism

(2010) Wei Yang   QUARTERLY REVIEWS OF BIOPHYSICS

An equivalent metal ion in one- and two-metal-ion catalysis

(2008) Wei Yang   NATURE STRUCTURAL & MOLECULAR BIOLOGY