标题
R-loop formation and conformational activation mechanisms of Cas9
作者
关键词
-
出版物
NATURE
Volume 609, Issue 7925, Pages 191-196
出版商
Springer Science and Business Media LLC
发表日期
2022-08-25
DOI
10.1038/s41586-022-05114-0
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- Structural basis for mismatch surveillance by CRISPR–Cas9
- (2022) Jack P. K. Bravo et al. NATURE
- CRISPR–Cas9 bends and twists DNA to read its sequence
- (2022) Joshua C. Cofsky et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement
- (2021) Evan A. Boyle et al. Science Advances
- 3D variability analysis: Resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM
- (2021) Ali Punjani et al. JOURNAL OF STRUCTURAL BIOLOGY
- Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells
- (2021) Paul D. Donohoue et al. MOLECULAR CELL
- Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling
- (2020) Ivan E. Ivanov et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes
- (2020) Liyang Zhang et al. NUCLEIC ACIDS RESEARCH
- Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
- (2020) Andrew V. Anzalone et al. NATURE BIOTECHNOLOGY
- CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
- (2020) Cicera R. Lazzarotto et al. NATURE BIOTECHNOLOGY
- Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
- (2020) Stephen K. Jones et al. NATURE BIOTECHNOLOGY
- UCSF ChimeraX : Structure visualization for researchers, educators, and developers
- (2020) Eric F. Pettersen et al. PROTEIN SCIENCE
- Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
- (2020) Yifei Zhang et al. Nature Catalysis
- Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction
- (2020) Ali Punjani et al. NATURE METHODS
- DNA stretching induces Cas9 off-target activity
- (2019) Matthew D. Newton et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Web 3DNA 2.0 for the analysis, visualization, and modeling of 3D nucleic acid structures
- (2019) Shuxiang Li et al. NUCLEIC ACIDS RESEARCH
- Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9
- (2019) Xing Zhu et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix
- (2019) Dorothee Liebschner et al. Acta Crystallographica Section D-Structural Biology
- Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States
- (2019) Wei Sun et al. MOLECULAR CELL
- Target Specificity of Cas9 Nuclease via DNA Rearrangement Regulated by the REC2 Domain
- (2018) Keewon Sung et al. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET
- (2018) Mengyi Yang et al. Cell Reports
- Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
- (2017) Janice S. Chen et al. NATURE
- cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination
- (2017) Ali Punjani et al. NATURE METHODS
- Mapping the genomic landscape of CRISPR–Cas9 cleavage
- (2017) Peter Cameron et al. NATURE METHODS
- Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation
- (2017) Vladimir Mekler et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- MolProbity: More and better reference data for improved all-atom structure validation
- (2017) Christopher J. Williams et al. PROTEIN SCIENCE
- A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9
- (2017) Yavuz S. Dagdas et al. Science Advances
- Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9
- (2016) Carolin Anders et al. MOLECULAR CELL
- High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
- (2016) Benjamin P. Kleinstiver et al. NATURE
- Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
- (2016) John G Doench et al. NATURE BIOTECHNOLOGY
- Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
- (2016) F. Jiang et al. SCIENCE
- Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
- (2016) Digvijay Singh et al. Nature Communications
- Two Distinct DNA Binding Modes Guide Dual Roles of a CRISPR-Cas Protein Complex
- (2015) Timothy R. Blosser et al. MOLECULAR CELL
- Conformational control of DNA target cleavage by CRISPR–Cas9
- (2015) Samuel H. Sternberg et al. NATURE
- A Cas9-guide RNA complex preorganized for target DNA recognition
- (2015) F. Jiang et al. SCIENCE
- Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
- (2014) Hiroshi Nishimasu et al. CELL
- Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
- (2014) Carolin Anders et al. NATURE
- DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
- (2014) Samuel H. Sternberg et al. NATURE
- Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
- (2014) Cem Kuscu et al. NATURE BIOTECHNOLOGY
- GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
- (2014) Shengdar Q Tsai et al. NATURE BIOTECHNOLOGY
- Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes
- (2014) Mark D. Szczelkun et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target
- (2014) S. Mulepati et al. SCIENCE
- DNA targeting specificity of RNA-guided Cas9 nucleases
- (2013) Patrick D Hsu et al. NATURE BIOTECHNOLOGY
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- (2013) L. Cong et al. SCIENCE
- RNA-programmed genome editing in human cells
- (2013) Martin Jinek et al. eLife
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- Data processing and analysis with theautoPROCtoolbox
- (2011) Clemens Vonrhein et al. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
- (2011) Rimantas Sapranauskas et al. NUCLEIC ACIDS RESEARCH
- Features and development ofCoot
- (2010) P. Emsley et al. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
- (2010) Josiane E. Garneau et al. NATURE
- Jalview Version 2--a multiple sequence alignment editor and analysis workbench
- (2009) A. M. Waterhouse et al. BIOINFORMATICS
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