Cryo-EM structure of the spliceosome immediately after branching

Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites

(2016) Daniel R. Semlow et al.   CELL

CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast

(2016) Thi Hoang Duong Nguyen et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution

(2016) Thi Hoang Duong Nguyen et al.   NATURE

The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis

(2016) R. Wan et al.   SCIENCE

113Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p

(2015) Anne-Marie M. van Roon et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Semi-automated selection of cryo-EM particles in RELION-1.3

(2015) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

The architecture of the spliceosomal U4/U6.U5 tri-snRNP

(2015) Thi Hoang Duong Nguyen et al.   NATURE

The I-TASSER Suite: protein structure and function prediction

(2015) Jianyi Yang et al.   NATURE METHODS

The exon junction complex as a node of post-transcriptional networks

(2015) Hervé Le Hir et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET

(2015) John W. Hardin et al.   NUCLEIC ACIDS RESEARCH

Structure of a yeast spliceosome at 3.6-angstrom resolution

(2015) Chuangye Yan et al.   SCIENCE

Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions

(2015) Alan Brown et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Evidence for a group II intron–like catalytic triplex in the spliceosome

(2014) Sebastian M Fica et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RNA polymerase II termination involves C-terminal-domain tyrosine dephosphorylation by CPF subunit Glc7

(2014) Amelie Schreieck et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information

(2014) Marco Biasini et al.   NUCLEIC ACIDS RESEARCH

A homolog of lariat-debranching enzyme modulates turnover of branched RNA

(2014) Stephen M. Garrey et al.   RNA

Endogenous U2{middle dot}U5{middle dot}U6 snRNA complexes in S. pombe are intron lariat spliceosomes

(2014) W. Chen et al.   RNA

Conformation-independent structural comparison of macromolecules withProSMART

(2014) Robert A. Nicholls et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Crystal structure of Prp8 reveals active site cavity of the spliceosome

(2013) Wojciech P. Galej et al.   NATURE

Quantifying the local resolution of cryo-EM density maps

(2013) Alp Kucukelbir et al.   NATURE METHODS

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing

(2013) Ramya Krishnan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system

(2013) T. Ohrt et al.   RNA

Structural Basis of Brr2-Prp8 Interactions and Implications for U5 snRNP Biogenesis and the Spliceosome Active Site

(2013) Thi Hoang Duong Nguyen et al.   STRUCTURE

High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy

(2013) Shaoxia Chen et al.   ULTRAMICROSCOPY

Visualizing Group II Intron Catalysis through the Stages of Splicing

(2012) Marco Marcia et al.   CELL

Structure, function and regulation of spliceosomal RNA helicases

(2012) Olivier Cordin et al.   CURRENT OPINION IN CELL BIOLOGY

Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre

(2012) Nicolas Rasche et al.   EMBO JOURNAL

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Link of NTR-Mediated Spliceosome Disassembly with DEAH-Box ATPases Prp2, Prp16, and Prp22

(2012) Hsin-Chou Chen et al.   MOLECULAR AND CELLULAR BIOLOGY

Prevention of overfitting in cryo-EM structure determination

(2012) Sjors H W Scheres et al.   NATURE METHODS

Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly

(2012) Isabelle Barbosa et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Human spliceosomal protein CWC22 plays a role in coupling splicing to exon junction complex deposition and nonsense-mediated decay

(2012) A. Alexandrov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CWC22 Connects Pre-mRNA Splicing and Exon Junction Complex Assembly

(2012) Anna-Lena Steckelberg et al.   Cell Reports

Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition

(2011) Peilong Lu et al.   BIOCHEMICAL JOURNAL

Cross-linking, ligation, and sequencing of hybrids reveals RNA-RNA interactions in yeast

(2011) G. Kudla et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Prp43p contains a processive helicase structural architecture with a specific regulatory domain

(2010) Hélène Walbott et al.   EMBO JOURNAL

Conformational dynamics of single pre-mRNA molecules during in vitro splicing

(2010) John Abelson et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps

(2010) C.-K. Tseng et al.   RNA

Cwc25 Is a Novel Splicing Factor Required after Prp2 and Yju2 To Facilitate the First Catalytic Reaction

(2009) Y.-F. Chiu et al.   MOLECULAR AND CELLULAR BIOLOGY

The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome

(2009) Patrizia Fabrizio et al.   MOLECULAR CELL

Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components

(2009) Zbigniew Warkocki et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome

(2009) Rebecca M Voorhees et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome

(2009) R. J. Grainger et al.   RNA

“Nought May Endure but Mutability”: Spliceosome Dynamics and the Regulation of Splicing

(2008) Duncan J. Smith et al.   MOLECULAR CELL

A Conformational Rearrangement in the Spliceosome Sets the Stage for Prp22-Dependent mRNA Release

(2008) Beate Schwer   MOLECULAR CELL

Isolation of an active step I spliceosome and composition of its RNP core

(2008) Sergey Bessonov et al.   NATURE