Genetic engineering of modular PKSs: from combinatorial biosynthesis to synthetic biology

Polyketide synthase and non-ribosomal peptide synthetase thioesterase selectivity: logic gate or a victim of fate?

(2016) Mark E. Horsman et al.   NATURAL PRODUCT REPORTS

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Gene Replacement for the Generation of Designed Novel Avermectin Derivatives with Enhanced Acaricidal and Nematicidal Activities

(2015) Jun Huang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Evaluating Ketoreductase Exchanges as a Means of Rationally Altering Polyketide Stereochemistry

(2015) Thibault Annaval et al.   CHEMBIOCHEM

Freedom and Constraint in Engineered Noncolinear Polyketide Assembly Lines

(2015) Yuki Sugimoto et al.   CHEMISTRY & BIOLOGY

Evolution of Efficient Modular Polyketide Synthases by Homologous Recombination

(2015) Joseph A. Chemler et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the Biologist’s Toolkit with CRISPR-Cas9

(2015) Samuel H. Sternberg et al.   MOLECULAR CELL

Metabolic and evolutionary origin of actin-binding polyketides from diverse organisms

(2015) Reiko Ueoka et al.   Nature Chemical Biology

Discovery of microbial natural products by activation of silent biosynthetic gene clusters

(2015) Peter J. Rutledge et al.   NATURE REVIEWS MICROBIOLOGY

Structural and evolutionary relationships of “AT-less” type I polyketide synthase ketosynthases

(2015) Jeremy R. Lohman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Decoding and reprogramming complex polyketide assembly lines: prospects for synthetic biology

(2015) Christian Hertweck   TRENDS IN BIOCHEMICAL SCIENCES

Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

(2015) Yongxin Li et al.   Scientific Reports

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

(2015) Yaojun Tong et al.   ACS Synthetic Biology

Rational Design of Modular Polyketide Synthases: Morphing the Aureothin Pathway into a Luteoreticulin Assembly Line

(2014) Yuki Sugimoto et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bacteriophage recombination systems and biotechnical applications

(2014) Nafiseh Nafissi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Comparative Analysis of the Substrate Specificity of trans- versus cis-Acyltransferases of Assembly Line Polyketide Synthases

(2014) Briana J. Dunn et al.   BIOCHEMISTRY

Progress challenges and opportunities for the re-engineering of trans-AT polyketide synthases

(2014) M. Till et al.   BIOTECHNOLOGY LETTERS

Predicted Incorporation of Non-native Substrates by a Polyketide Synthase Yields Bioactive Natural Product Derivatives

(2014) Kenny Bravo-Rodriguez et al.   CHEMBIOCHEM

Biosynthesis of Crocacin Involves an Unusual Hydrolytic Release Domain Showing Similarity to Condensation Domains

(2014) Stefan Müller et al.   CHEMISTRY & BIOLOGY

Narrowing the gap between the promise and reality of polyketide synthases as a synthetic biology platform

(2014) Sean Poust et al.   CURRENT OPINION IN BIOTECHNOLOGY

Elucidation of the Cryptic Epimerase Activity of Redox-Inactive Ketoreductase Domains from Modular Polyketide Synthases by Tandem Equilibrium Isotope Exchange

(2014) Ashish Garg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Natural product and natural product derived drugs in clinical trials

(2014) Mark S. Butler et al.   NATURAL PRODUCT REPORTS

Structure of a modular polyketide synthase

(2014) Somnath Dutta et al.   NATURE

Structural rearrangements of a polyketide synthase module during its catalytic cycle

(2014) Jonathan R. Whicher et al.   NATURE

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

A Hybrid Non-Ribosomal Peptide/Polyketide Synthetase Containing Fatty-Acyl Ligase (FAAL) Synthesizes the β-Amino Fatty Acid Lipopeptides Puwainaphycins in the Cyanobacterium Cylindrospermum alatosporum

(2014) Jan Mareš et al.   PLoS One

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

A Systematic Computational Analysis of Biosynthetic Gene Cluster Evolution: Lessons for Engineering Biosynthesis

(2014) Marnix H. Medema et al.   PLoS Computational Biology

Discovering new bioactive molecules from microbial sources

(2014) Paolo Monciardini et al.   Microbial Biotechnology

Multifactorial Control of Iteration Events in a Modular Polyketide Assembly Line

(2013) Benjamin Busch et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Enzymatic Domain for the Formation of Cyclic Ethers in Complex Polyketides

(2013) Petra Pöplau et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Multiplexing of Combinatorial Chemistry in Antimycin Biosynthesis: Expansion of Molecular Diversity and Utility

(2013) Yan Yan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Broad Substrate Specificity of the Loading Didomain of the Lipomycin Polyketide Synthase

(2013) Satoshi Yuzawa et al.   BIOCHEMISTRY

Mechanism and Specificity of an Acyltransferase Domain from a Modular Polyketide Synthase

(2013) Briana J. Dunn et al.   BIOCHEMISTRY

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Structural and Stereochemical Analysis of a Modular Polyketide Synthase Ketoreductase Domain Required for the Generation of a cis-Alkene

(2013) Shilah A. Bonnett et al.   CHEMISTRY & BIOLOGY

Cyanobacterial Polyketide Synthase Docking Domains: A Tool for Engineering Natural Product Biosynthesis

(2013) Jonathan R. Whicher et al.   CHEMISTRY & BIOLOGY

Steps towards the synthetic biology of polyketide biosynthesis

(2013) Matthew Cummings et al.   FEMS MICROBIOLOGY LETTERS

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Computational identification and analysis of orphan assembly-line polyketide synthases

(2013) Robert V O'Brien et al.   JOURNAL OF ANTIBIOTICS

Biosynthesis and pathway engineering of antifungal polyene macrolides in actinomycetes

(2013) Dekun Kong et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Microbial genome mining for accelerated natural products discovery: is a renaissance in the making?

(2013) Brian O. Bachmann et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Coupled Methyl Group Epimerization and Reduction by Polyketide Synthase Ketoreductase Domains. Ketoreductase-Catalyzed Equilibrium Isotope Exchange

(2013) Ashish Garg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering the acyltransferase substrate specificity of assembly line polyketide synthases

(2013) B. J. Dunn et al.   Journal of the Royal Society Interface

Recent advances in the heterologous expression of microbial natural product biosynthetic pathways

(2013) Sarah E. Ongley et al.   NATURAL PRODUCT REPORTS

Vinylogous chain branching catalysed by a dedicated polyketide synthase module

(2013) Tom Bretschneider et al.   NATURE

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Phage P1-Derived Artificial Chromosomes Facilitate Heterologous Expression of the FK506 Gene Cluster

(2013) Adam C. Jones et al.   PLoS One

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

(2013) Z. Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expanding the Fluorine Chemistry of Living Systems Using Engineered Polyketide Synthase Pathways

(2013) M. C. Walker et al.   SCIENCE

Engineered Streptomyces avermitilis Host for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites

(2013) Mamoru Komatsu et al.   ACS Synthetic Biology

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

(2012) Irina Koryakina et al.   ACS Chemical Biology

Minimally Invasive Mutagenesis Gives Rise to a Biosynthetic Polyketide Library

(2012) Susanna Kushnir et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Merging Chemical Synthesis and Biosynthesis: A New Chapter in the Total Synthesis of Natural Products and Natural Product Libraries

(2012) Andreas Kirschning et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Establishing a Toolkit for Precursor-Directed Polyketide Biosynthesis: Exploring Substrate Promiscuities of Acid-CoA Ligases

(2012) Maybelle Kho Go et al.   BIOCHEMISTRY

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

Divergence of multimodular polyketide synthases revealed by a didomain structure

(2012) Jianting Zheng et al.   Nature Chemical Biology

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

β-Glucuronidase as a Sensitive and Versatile Reporter in Actinomycetes

(2011) Maksym Myronovskyi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of doramectin by rational engineering of the avermectin biosynthetic pathway

(2011) Jian-Bo Wang et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Enzymatic Extender Unit Generation for In Vitro Polyketide Synthase Reactions: Structural and Func-tional Showcasing of Streptomyces coelicolor MatB

(2011) Amanda J. Hughes et al.   CHEMISTRY & BIOLOGY

Terminal Alkene Formation by the Thioesterase of Curacin A Biosynthesis

(2011) Jennifer J. Gehret et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Beyond ethylmalonyl-CoA: The functional role of crotonyl-CoAcarboxylase/reductase homologs in expanding polyketide diversity

(2011) Micheal C. Wilson et al.   NATURAL PRODUCT REPORTS

Insights into the stereospecificity of ketoreduction in a modular polyketide synthase

(2011) David H. Kwan et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Identification of a bioactive 51-membered macrolide complex by activation of a silent polyketide synthase in Streptomyces ambofaciens

(2011) L. Laureti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Approaches to Capturing and Designing Biologically Active Small Molecules Produced by Uncultured Microbes

(2010) Jörn Piel   Annual Review of Microbiology

Engineered polyketide biosynthesis and biocatalysis in Escherichia coli

(2010) Xue Gao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

(2010) Jeffrey H. Kim et al.   BIOPOLYMERS

Complete Biosynthesis of Erythromycin A and Designed Analogs Using E. coli as a Heterologous Host

(2010) Haoran Zhang et al.   CHEMISTRY & BIOLOGY

Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters

(2010) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Post-PKS tailoring steps in natural product-producing actinomycetes from the perspective of combinatorial biosynthesis

(2010) Carlos Olano et al.   NATURAL PRODUCT REPORTS

Biosynthesis of polyketides by trans-AT polyketide synthases

(2010) Jörn Piel   NATURAL PRODUCT REPORTS

Methods and options for the heterologous production of complex natural products

(2010) Haoran Zhang et al.   NATURAL PRODUCT REPORTS

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis for Binding Specificity between Subclasses of Modular Polyketide Synthase Docking Domains

(2009) Tonia J. Buchholz et al.   ACS Chemical Biology

Functional Dissection of a Multimodular Polypeptide of the Pikromycin Polyketide Synthase into Monomodules by Using a Matched Pair of Heterologous Docking Domains

(2009) John Yan et al.   CHEMBIOCHEM

The Biochemical Basis for Stereochemical Control in Polyketide Biosynthesis

(2009) Chiara R. Valenzano et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Metamorphic enzyme assembly in polyketide diversification

(2009) Liangcai Gu et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

A Polylinker Approach to Reductive Loop Swaps in Modular Polyketide Synthases

(2008) Laurenz Kellenberger et al.   CHEMBIOCHEM

In Vivo and In Vitro Trans-Acylation by BryP, the Putative Bryostatin Pathway Acyltransferase Derived from an Uncultured Marine Symbiont

(2008) Nicole B. Lopanik et al.   CHEMISTRY & BIOLOGY

Prediction and Manipulation of the Stereochemistry of Enoylreduction in Modular Polyketide Synthases

(2008) David H. Kwan et al.   CHEMISTRY & BIOLOGY

DKxanthene Biosynthesis—Understanding the Basis for Diversity-Oriented Synthesis in Myxobacterial Secondary Metabolism

(2008) Peter Meiser et al.   CHEMISTRY & BIOLOGY

Stereochemical Determination and Complex Biosynthetic Assembly of Etnangien, a Highly Potent RNA Polymerase Inhibitor from the Myxobacterium Sorangium cellulosum

(2008) Dirk Menche et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Isoprenoid-like alkylations in polyketide biosynthesis

(2008) Christopher T. Calderone   NATURAL PRODUCT REPORTS

Exploiting the mosaic structure of trans-acyltransferase polyketide synthases for natural product discovery and pathway dissection

(2008) TuAnh Nguyen et al.   NATURE BIOTECHNOLOGY

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Substrate specificity of the acyl transferase domains of EpoC from the epothilone polyketide synthase

(2007) Hrvoje Petković et al.   ORGANIC & BIOMOLECULAR CHEMISTRY