Heterologous Single-Strand DNA-Annealing and Binding Protein Enhance CRISPR-Based Genome Editing Efficiency in Komagataella phaffii

A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris

(2023) Xiang Wang et al.   Synthetic and Systems Biotechnology

An improved CRISPRi system in Pichia pastoris

(2023) Shujing Qiao et al.   Synthetic and Systems Biotechnology

Efficient Bioproduction of Human Milk Alpha-Lactalbumin in Komagataella phaffii

(2022) Mengting Deng et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms

(2022) Jucan Gao et al.   ACS Synthetic Biology

A programmable high-expression yeast platform responsive to user-defined signals

(2022) Qi Liu et al.   Science Advances

Can recombinant milk proteins replace those produced by animals?

(2022) Kasper Hettinga et al.   CURRENT OPINION IN BIOTECHNOLOGY

Going beyond the limit: Increasing global translation activity leads to increased productivity of recombinant secreted proteins in Pichia pastoris

(2022) Jennifer Staudacher et al.   METABOLIC ENGINEERING

Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

(2022) Kun Zhang et al.   Microbial Cell Factories

Yeast transcriptional device libraries enable precise synthesis of value-added chemicals from methanol

(2022) Qiaoyun Zhu et al.   NUCLEIC ACIDS RESEARCH

Characterizing the portability of phage-encoded homologous recombination proteins

(2021) Gabriel T. Filsinger et al.   Nature Chemical Biology

Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens

(2021) Maierdan Palihati et al.   CURRENT GENETICS

Efficient Secretory Expression and Purification of Food-Grade Porcine Myoglobin in Komagataella phaffii

(2021) Bohan Zhang et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

(2021) Peng Cai et al.   NUCLEIC ACIDS RESEARCH

A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris

(2021) Xihao Liao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A Novel and Efficient Genome Editing Tool Assisted by CRISPR-Cas12a/Cpf1 for Pichia pastoris

(2021) Xinying Zhang et al.   ACS Synthetic Biology

Improved bacterial recombineering by parallelized protein discovery

(2020) Timothy M. Wannier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR-derived genome editing technologies for metabolic engineering

(2020) Keiji Nishida et al.   METABOLIC ENGINEERING

Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

(2020) Yoichiro Ito et al.   NUCLEIC ACIDS RESEARCH

Microbial single-strand annealing proteins enable CRISPR gene-editing tools with improved knock-in efficiencies and reduced off-target effects

(2020) Chengkun Wang et al.   NUCLEIC ACIDS RESEARCH

Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris

(2019) Yang Gu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Tailoring the Saccharomyces cerevisiae endoplasmic reticulum for functional assembly of terpene synthesis pathway

(2019) Jae-Eung Kim et al.   METABOLIC ENGINEERING

The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2

(2019) Thomas Gassler et al.   NATURE BIOTECHNOLOGY

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Effect of Plasmid Design and Type of Integration Event on Recombinant Protein Expression in Pichia pastoris

(2018) Thomas Vogl et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Role of homologous recombination genes RAD51 , RAD52 , and RAD59 in the repair of lesions caused by γ-radiation to cycling and G2/M-arrested cells of Candida albicans

(2018) Alberto Bellido et al.   CELLULAR MICROBIOLOGY

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Compact multi-enzyme pathways in P. pastoris

(2015) Martina Geier et al.   CHEMICAL COMMUNICATIONS

Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination

(2015) Gur Pines et al.   ACS Synthetic Biology

Applications of recombinant Pichia pastoris in the healthcare industry

(2014) Daniel Weinacker et al.   BRAZILIAN JOURNAL OF MICROBIOLOGY

Recombinants proteins for industrial uses: utilization of Pichia pastoris expression system

(2013) Claudia Rabert et al.   BRAZILIAN JOURNAL OF MICROBIOLOGY

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology

Engineering of protein secretion in yeast: strategies and impact on protein production

(2010) Alimjan Idiris et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response

(2009) Sebastian Schuck et al.   JOURNAL OF CELL BIOLOGY

Dimerization of yeast transcription factors Ino2 and Ino4 is regulated by precursors of phospholipid biosynthesis mediated by Opi1 repressor

(2008) Jacqueline Kumme et al.   CURRENT GENETICS