A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris

Engineering Pichia pastoris to improve S‐adenosyl‐ l ‐methionine production using systems metabolic strategies

(2020) Xiulin Qin et al.   BIOTECHNOLOGY AND BIOENGINEERING

Pichia pastoris : A highly successful expression system for optimal synthesis of heterologous proteins

(2020) Mohsen Karbalaei et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

(2020) Nicholas S. McCarty et al.   Nature Communications

Guide RNA Engineering Enables Dual Purpose CRISPR-Cpf1 for Simultaneous Gene Editing and Gene Regulation in Yarrowia lipolytica

(2020) Adithya Ramesh et al.   ACS Synthetic Biology

High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris

(2020) Yankun Yang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Metabolic engineering of Pichia pastoris for malic acid production from methanol

(2020) Feng Guo et al.   BIOTECHNOLOGY AND BIOENGINEERING

A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae

(2020) Chang Dong et al.   ACS Synthetic Biology

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

(2019) Yueping Zhang et al.   Nature Communications

Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris

(2019) Xihao Liao et al.   Biotechnology for Biofuels

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

(2019) Qi Liu et al.   Microbial Cell Factories

Combined genome editing and transcriptional repression for metabolic pathway engineering in Corynebacterium glutamicum using a catalytically active Cas12a

(2019) Wei Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Current advances in engineering tools for Pichia pastoris

(2019) Jasmin E Fischer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

(2019) Neil C. Dalvie et al.   ACS Synthetic Biology

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris : A review

(2018) Zhiliang Yang et al.   BIOTECHNOLOGY ADVANCES

A CRISPR–dCas Toolbox for Genetic Engineering and Synthetic Biology

(2018) Xiaoshu Xu et al.   JOURNAL OF MOLECULAR BIOLOGY

Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol

(2018) Yiqi Liu et al.   METABOLIC ENGINEERING

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

(2018) Tianmin Wang et al.   Nature Communications

A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA

(2018) Yasuyuki Nakamura et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts

(2018) Laiyou Wang et al.   Biotechnology for Biofuels

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

A Modular Toolkit for Generating Pichia pastoris Secretion Libraries

(2017) Ulrike Obst et al.   ACS Synthetic Biology

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

(2015) James E Dahlman et al.   NATURE BIOTECHNOLOGY

Cas9 gRNA engineering for genome editing, activation and repression

(2015) Samira Kiani et al.   NATURE METHODS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115

(2013) Min Wu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Direct selection ofPichia pastoris expression strains using new G418 resistance vectors

(2008) Joan Lin-Cereghino et al.   YEAST