A Novel and Efficient Genome Editing Tool Assisted by CRISPR-Cas12a/Cpf1 for Pichia pastoris

Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

(2021) Jiaoqi Gao et al.   iScience

High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris

(2020) Yankun Yang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Development and Application of CRISPR/Cas in Microbial Biotechnology

(2020) Wentao Ding et al.   Frontiers in Bioengineering and Biotechnology

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

(2019) Yueping Zhang et al.   Nature Communications

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications

(2019) Peng Cai et al.   Microbial Cell Factories

Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris

(2019) Yang Gu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast

(2019) Yiqi Liu et al.   METABOLIC ENGINEERING

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

(2019) Qi Liu et al.   Microbial Cell Factories

Production of lycopene by metabolically engineered Pichia pastoris

(2019) Xinying Zhang et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

(2019) Neil C. Dalvie et al.   ACS Synthetic Biology

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Advances in Industrial Biotechnology Using CRISPR-Cas Systems

(2018) Paul D. Donohoue et al.   TRENDS IN BIOTECHNOLOGY

Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis

(2018) Chuixing Kong et al.   Scientific Reports

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system

(2017) Feng Peng et al.   Microbial Cell Factories

FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae

(2017) Michal A. Świat et al.   NUCLEIC ACIDS RESEARCH

Automated multiplex genome-scale engineering in yeast

(2017) Tong Si et al.   Nature Communications

Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

(2017) Cheng Li et al.   Scientific Reports

Large fragment deletion using a CRISPR/Cas9 system in Saccharomyces cerevisiae

(2016) Huanhuan Hao et al.   ANALYTICAL BIOCHEMISTRY

Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris

(2016) Chengcheng Zhao et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2016) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Metabolic engineering ofPichia pastoristo produce ricinoleic acid, a hydroxy fatty acid of industrial importance

(2015) Dauenpen Meesapyodsuk et al.   JOURNAL OF LIPID RESEARCH

Bacteriophage T7 RNA polymerase-based expression in Pichia pastoris

(2013) Birgit Hobl et al.   PROTEIN EXPRESSION AND PURIFICATION

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH