An improved CRISPRi system in Pichia pastoris

Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms

(2022) Jucan Gao et al.   ACS Synthetic Biology

A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli

(2022) Ming Zhao et al.   Synthetic and Systems Biotechnology

Biosynthesis of tetrahydropapaverine and semisynthesis of papaverine in yeast

(2022) Osman K. Jamil et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Inducible expression of large gRNA arrays for multiplexed CRISPRai applications

(2022) William M. Shaw et al.   Nature Communications

Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol

(2022) Rui Hou et al.   Synthetic and Systems Biotechnology

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

(2021) Peng Cai et al.   NUCLEIC ACIDS RESEARCH

Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids

(2021) Lixia Fang et al.   Nature Communications

A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris

(2021) Xihao Liao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

(2020) Nicholas S. McCarty et al.   Nature Communications

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

(2020) Lun Yao et al.   Nature Communications

CRISPR-based gene expression control for synthetic gene circuits

(2020) Javier Santos-Moreno et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Expression of recombinant EGFP provides insight into foreign gene‐expression differences between Mut+ and MutS strains of Pichia pastoris

(2019) Chrispian W. Theron et al.   YEAST

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications

(2019) Peng Cai et al.   Microbial Cell Factories

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

(2019) Neil C. Dalvie et al.   ACS Synthetic Biology

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris : A review

(2018) Zhiliang Yang et al.   BIOTECHNOLOGY ADVANCES

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays

(2018) Morito Kurata et al.   PLoS One

Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

(2018) Thomas Vogl et al.   Nature Communications

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation Using Csy4 in Saccharomyces cerevisiae

(2017) Raphael Ferreira et al.   ACS Synthetic Biology

High-efficiency CRISPR/Cas9 multiplex gene editing using the glycine tRNA-processing system-based strategy in maize

(2016) Weiwei Qi et al.   BMC BIOTECHNOLOGY

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

(2016) Aliaksandra Radzisheuskaya et al.   NUCLEIC ACIDS RESEARCH

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

(2016) Cory M. Schwartz et al.   ACS Synthetic Biology

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

(2015) Levi G. Lowder et al.   PLANT PHYSIOLOGY

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

RNA-guided transcriptional regulationin plantavia synthetic dCas9-based transcription factors

(2014) Agnieszka Piatek et al.   PLANT BIOTECHNOLOGY JOURNAL

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology

Csy4 relies on an unusual catalytic dyad to position and cleave CRISPR RNA

(2012) Rachel E Haurwitz et al.   EMBO JOURNAL

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease

(2010) Rachel E. Haurwitz et al.   SCIENCE

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH