How colonization bottlenecks, tissue niches, and transmission strategies shape protozoan infections

Drug discovery for parasitic diseases: powered by technology, enabled by pharmacology, informed by clinical science

(2023) Srinivasa P.S. Rao et al.   TRENDS IN PARASITOLOGY

Host genetics highlights IFN-γ-dependent Toxoplasma genes encoding secreted and non-secreted virulence factors in in vivo CRISPR screens

(2023) Yuta Tachibana et al.   Cell Reports

A positive feedback loop controls Toxoplasma chronic differentiation

(2023) M. Haley Licon et al.   Nature Microbiology

Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept

(2023) Cécile Minet et al.   EXPERIMENTAL PARASITOLOGY

CD4+ T cells regulate sickness-induced anorexia and fat wasting during a chronic parasitic infection

(2023) Samuel E. Redford et al.   Cell Reports

Intraluminal neutrophils limit epithelium damage by reducing pathogen assault on intestinal epithelial cells during Salmonella gut infection

(2023) Ersin Gül et al.   PLoS Pathogens

Deep Visual Proteomics defines single-cell identity and heterogeneity

(2022) Andreas Mund et al.   NATURE BIOTECHNOLOGY

Microbiota and maintenance of skin barrier function

(2022) Tamia A. Harris-Tryon et al.   SCIENCE

High-resolution Slide-seqV2 spatial transcriptomics enables discovery of disease-specific cell neighborhoods and pathways

(2022) Jamie L. Marshall et al.   iScience

Barcoding Genetically Distinct Plasmodium falciparum Strains for Comparative Assessment of Fitness and Antimalarial Drug Resistance

(2022) Manuela Carrasquilla et al.   mBio

Differential plasticity and fate of brain-resident and recruited macrophages during the onset and resolution of neuroinflammation

(2022) Karen De Vlaminck et al.   IMMUNITY

DNA barcoding, an effective tool for species identification: a review

(2022) Sandeep Antil et al.   MOLECULAR BIOLOGY REPORTS

Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing

(2022) Jocelyn Y. Kishi et al.   NATURE METHODS

Cellular barcoding to decipher clonal dynamics in disease

(2022) Vijay G. Sankaran et al.   SCIENCE

Single cell and spatial transcriptomic analyses reveal microglia-plasma cell crosstalk in the brain during Trypanosoma brucei infection

(2022) Juan F. Quintana et al.   Nature Communications

VSGs Expressed during Natural T. b. gambiense Infection Exhibit Extensive Sequence Divergence and a Subspecies-Specific Bias towards Type B N-Terminal Domains

(2022) Jaime So et al.   mBio

Impact of pulmonary African trypanosomes on the immunology and function of the lung

(2022) Dorien Mabille et al.   Nature Communications

Slow growing behavior in African trypanosomes during adipose tissue colonization

(2022) Sandra Trindade et al.   Nature Communications

Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain

(2022) Michael A Kovacs et al.   eLife

Fatty acid uptake in Trypanosoma brucei: Host resources and possible mechanisms

(2022) Nava Raj Poudyal et al.   Frontiers in Cellular and Infection Microbiology

Imaging host–pathogen interactions using epithelial and bacterial cell infection models

(2021) Amber J. Park et al.   JOURNAL OF CELL SCIENCE

Toxoplasma gondii infection and its implications within the central nervous system

(2021) Sumit K. Matta et al.   NATURE REVIEWS MICROBIOLOGY

Mapping immune variation and var gene switching in naive hosts infected with Plasmodium falciparum

(2021) Kathryn Milne et al.   eLife

Clinical characteristics and outcomes of toxoplasmosis among transplant recipients at two US academic medical centers.

(2021) Ruth O. Adekunle et al.   Transplant Infectious Disease

Diversity of tsetse flies and trypanosome species circulating in the area of Lake Iro in southeastern Chad

(2021) Djoukzoumka Signaboubo et al.   Parasites & Vectors

The gut vascular barrier: a new player in the gut–liver–brain axis

(2021) Paola Brescia et al.   TRENDS IN MOLECULAR MEDICINE

Refined Quantification of Infection Bottlenecks and Pathogen Dissemination with STAMPR

(2021) Karthik Hullahalli et al.   mSystems

Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries

(2021) Gabriela C Olivera et al.   eLife

Pathogen clonal expansion underlies multiorgan dissemination and organ-specific outcomes during murine systemic infection

(2021) Karthik Hullahalli et al.   eLife

The Infectious Dose Shapes Vibrio cholerae Within-Host Dynamics

(2021) Aaron Nicholas Gillman et al.   mSystems

Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression

(2020) Annika Hausmann et al.   Mucosal Immunology

Molecular identification of different trypanosome species in tsetse flies caught in the wildlife reserve of Santchou in the western region of Cameroon

(2020) Cyrille Nguemnang Kamdem et al.   PARASITOLOGY RESEARCH

Systemic infection facilitates transmission of Pseudomonas aeruginosa in mice

(2020) Kelly E. R. Bachta et al.   Nature Communications

Import of Aspartate and Malate by DcuABC Drives H2/Fumarate Respiration to Promote Initial Salmonella Gut-Lumen Colonization in Mice

(2020) Bidong D. Nguyen et al.   Cell Host & Microbe

To the Skin and Beyond: The Immune Response to African Trypanosomes as They Enter and Exit the Vertebrate Host

(2020) Omar A. Alfituri et al.   Frontiers in Immunology

Bar-seq strategies for the LeishGEdit toolbox

(2020) Tom Beneke et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages

(2020) Yifan Wang et al.   Nature Communications

T. gondii infection induces IL-1R dependent chronic cachexia and perivascular fibrosis in the liver and skeletal muscle

(2020) Stephanie J. Melchor et al.   Scientific Reports

Sleeping Sickness: A Tale of Two Clocks

(2020) Filipa Rijo-Ferreira et al.   Frontiers in Cellular and Infection Microbiology

Structural, Functional, and Metabolic Alterations in Human Cerebrovascular Endothelial Cells during Toxoplasma gondii Infection and Amelioration by Verapamil In Vitro

(2020) Alaa T. Al-sandaqchi et al.   Microorganisms

From Entry to Early Dissemination—Toxoplasma gondii's Initial Encounter With Its Host

(2019) Estefania Delgado Betancourt et al.   Frontiers in Cellular and Infection Microbiology

Human African Trypanosomiasis

(2019) Emmanuel Bottieau et al.   INFECTIOUS DISEASE CLINICS OF NORTH AMERICA

Trypanosoma brucei brucei causes a rapid and persistent influx of neutrophils in the spleen of infected mice

(2019) Violette Deleeuw et al.   PARASITE IMMUNOLOGY

Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections

(2019) Tom Beneke et al.   PLoS Pathogens

Salmonella persisters promote the spread of antibiotic resistance plasmids in the gut

(2019) Erik Bakkeren et al.   NATURE

An Open-Format Enteroid Culture System for Interrogation of Interactions Between Toxoplasma gondii and the Intestinal Epithelium

(2019) Lisa Luu et al.   Frontiers in Cellular and Infection Microbiology

A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

(2019) Joanna Young et al.   Nature Communications

Automated Spatially Targeted Optical Microproteomics (autoSTOMP) to Determine Protein Complexity of Subcellular Structures

(2019) Bocheng Yin et al.   ANALYTICAL CHEMISTRY

A Multiorgan Trafficking Circuit Provides Purifying Selection of Listeria monocytogenes Virulence Genes

(2019) Alexander Louie et al.   mBio

Neutral barcoding of genomes reveals the dynamics of Salmonella colonization in cattle and their peripheral lymph nodes

(2018) Steffen Porwollik et al.   VETERINARY MICROBIOLOGY

The Neurotropic Parasite Toxoplasma gondii Induces Sustained Neuroinflammation with Microvascular Dysfunction in Infected Mice

(2018) Vanessa Estato et al.   AMERICAN JOURNAL OF PATHOLOGY

Neutrophils enhance early Trypanosoma brucei infection onset

(2018) Guy Caljon et al.   Scientific Reports

Trypanosoma brucei triggers a marked immune response in male reproductive organs

(2018) Tânia Carvalho et al.   PLoS Neglected Tropical Diseases

Cellular barcoding: lineage tracing, screening and beyond

(2018) Justus M. Kebschull et al.   NATURE METHODS

Within-host spatiotemporal dynamics of systemic Salmonella infection during and after antimicrobial treatment

(2017) O Rossi et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Human African trypanosomiasis

(2017) Philippe Büscher et al.   LANCET

Delineating neuroinflammation, parasite CNS invasion, and blood-brain barrier dysfunction in an experimental murine model of human African trypanosomiasis

(2017) Jean Rodgers et al.   METHODS

Deciphering the landscape of host barriers toListeria monocytogenesinfection

(2017) Ting Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Brains and Brawn: Toxoplasma Infections of the Central Nervous System and Skeletal Muscle

(2017) Elizabeth A. Wohlfert et al.   TRENDS IN PARASITOLOGY

RNAi screening identifies Trypanosoma brucei stress response protein kinases required for survival in the mouse

(2017) Fernando Fernandez-Cortes et al.   Scientific Reports

A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes

(2016) Saima M. Sidik et al.   CELL

Trypanosoma brucei Parasites Occupy and Functionally Adapt to the Adipose Tissue in Mice

(2016) Sandra Trindade et al.   Cell Host & Microbe

Visualization and analysis of gene expression in tissue sections by spatial transcriptomics

(2016) P. L. Stahl et al.   SCIENCE

Whole-organism lineage tracing by combinatorial and cumulative genome editing

(2016) Aaron McKenna et al.   SCIENCE

Trypanosoma brucei gambiense Infections in Mice Lead to Tropism to the Reproductive Organs, and Horizontal and Vertical Transmission

(2016) Nicolas Biteau et al.   PLoS Neglected Tropical Diseases

Trypanosoma brucei Invasion and T-Cell Infiltration of the Brain Parenchyma in Experimental Sleeping Sickness: Timing and Correlation with Functional Changes

(2016) Claudia Laperchia et al.   PLoS Neglected Tropical Diseases

Neurons are the Primary Target Cell for the Brain-Tropic Intracellular Parasite Toxoplasma gondii

(2016) Carla M. Cabral et al.   PLoS Pathogens

The Dermis as a Delivery Site of Trypanosoma brucei for Tsetse Flies

(2016) Guy Caljon et al.   PLoS Pathogens

Species-Specific Adaptations of Trypanosome Morphology and Motility to the Mammalian Host

(2016) Joel L. Bargul et al.   PLoS Pathogens

The skin is a significant but overlooked anatomical reservoir for vector-borne African trypanosomes

(2016) Paul Capewell et al.   eLife

Endothelial cells are a replicative niche for entry of Toxoplasma gondii to the central nervous system

(2016) Christoph Konradt et al.   Nature Microbiology

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

Single passage in mouse organs enhances the survival and spread ofSalmonella enterica

(2015) Richard Dybowski et al.   Journal of the Royal Society Interface

Sequence tag–based analysis of microbial population dynamics

(2015) Sören Abel et al.   NATURE METHODS

Light-sheet fluorescence microscopy for quantitative biology

(2015) Ernst H K Stelzer   NATURE METHODS

PlasmoGEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites

(2015) Frank Schwach et al.   NUCLEIC ACIDS RESEARCH

Toxoplasma gondiidissemination: a parasite's journey through the infected host

(2015) K. S. Harker et al.   PARASITE IMMUNOLOGY

The in vivo dynamics of antigenic variation in Trypanosoma brucei

(2015) M. R. Mugnier et al.   SCIENCE

A Pre-clinical Animal Model of Trypanosoma brucei Infection Demonstrating Cardiac Dysfunction

(2015) Charlotte S. McCarroll et al.   PLoS Neglected Tropical Diseases

Analysis of Bottlenecks in Experimental Models of Infection

(2015) Sören Abel et al.   PLoS Pathogens

Cyclical Appearance of African Trypanosomes in the Cerebrospinal Fluid: New Insights in How Trypanosomes Enter the CNS

(2014) Stefan Mogk et al.   PLoS One

Population Bottlenecks during the Infectious Cycle of the Lyme Disease Spirochete Borrelia burgdorferi

(2014) Ryan O. M. Rego et al.   PLoS One

Cecum Lymph Node Dendritic Cells Harbor Slow-Growing Bacteria Phenotypically Tolerant to Antibiotic Treatment

(2014) Patrick Kaiser et al.   PLOS BIOLOGY

Granulocytes Impose a Tight Bottleneck upon the Gut Luminal Pathogen Population during Salmonella Typhimurium Colitis

(2014) Lisa Maier et al.   PLoS Pathogens

Intraspecies Competition for Niches in the Distal Gut Dictate Transmission during Persistent Salmonella Infection

(2014) Lilian H. Lam et al.   PLoS Pathogens

Intraluminal Containment of Commensal Outgrowth in the Gut during Infection-Induced Dysbiosis

(2013) Michael J. Molloy et al.   Cell Host & Microbe

Replication and Distribution of Toxoplasma gondii in the Small Intestine after Oral Infection with Tissue Cysts

(2013) Beth Gregg et al.   INFECTION AND IMMUNITY

Within-Host Selection Is Limited by an Effective Population of Streptococcus pneumoniae during Nasopharyngeal Colonization

(2013) Yuan Li et al.   INFECTION AND IMMUNITY

Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread

(2013) J. L. Coombes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Concepts and Mechanisms: Crossing Host Barriers

(2013) K. S. Doran et al.   Cold Spring Harbor Perspectives in Medicine

Lymph Node Colonization Dynamics after Oral Salmonella Typhimurium Infection in Mice

(2013) Patrick Kaiser et al.   PLoS Pathogens

Early Invasion of Brain Parenchyma by African Trypanosomes

(2012) Ute Frevert et al.   PLoS One

Acute Gastrointestinal Infection Induces Long-Lived Microbiota-Specific T Cell Responses

(2012) T. W. Hand et al.   SCIENCE

Kinetics of Uropathogenic Escherichia coli Metapopulation Movement during Urinary Tract Infection

(2012) M. S. Walters et al.   mBio

Oral Infection with Signature-Tagged Listeria monocytogenes Reveals Organ-Specific Growth and Dissemination Routes in Guinea Pigs

(2011) Jody A. Melton-Witt et al.   INFECTION AND IMMUNITY

Intracellular transport of Toxoplasma gondii through the blood–brain barrier

(2010) Sabrina M. Lachenmaier et al.   JOURNAL OF NEUROIMMUNOLOGY

Bottlenecks and the Maintenance of Minor Genotypes during the Life Cycle of Trypanosoma brucei

(2010) Michael Oberle et al.   PLoS Pathogens

Gut Commensal Bacteria Direct a Protective Immune Response against Toxoplasma gondii

(2009) Alicia Benson et al.   Cell Host & Microbe

Quantitative phenotyping via deep barcode sequencing

(2009) A. M. Smith et al.   GENOME RESEARCH

Effective population size and patterns of molecular evolution and variation

(2009) Brian Charlesworth   NATURE REVIEWS GENETICS

Modelling within-Host Spatiotemporal Dynamics of Invasive Bacterial Disease

(2008) Andrew J Grant et al.   PLOS BIOLOGY