Article
Biology
Alexander S. Hauser, Charlotte Avet, Claire Normand, Arturo Mancini, Asuka Inoue, Michel Bouvier, David E. Gloriam
Summary: Two-thirds of human hormones and one-third of clinical drugs act on membrane receptors coupled to G proteins, but there are differences in the reported G protein couplings among recent large-scale datasets. This study presents a common coupling map that uncovers novel couplings, GPCR-G protein selectivity, and the comparison of co-coupling and co-expression of G proteins with phylogenetic relationships. These findings will advance receptor research and cellular signaling, and contribute to the development of safer drugs.
Review
Pharmacology & Pharmacy
Nathalie Fuentes, Morgan McCullough, Reynold A. Panettieri, Kirk M. Druey
Summary: Asthma is a prevalent disorder characterized by chronic lung inflammation and reversible airways obstruction, with features including increased airway narrowing, excessive mucus secretion, airway remodeling, and airways hyperresponsiveness. Environmental factors such as allergens, viruses, and air pollution can promote asthma, leading to symptoms like cough, wheezing, and shortness of breath. G-protein-coupled receptors play a central role in asthma, with various treatments targeting these receptors to regulate airway smooth muscle tone and lung inflammation.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Argha Mitra, Arijit Sarkar, Marton Richard Szabo, Attila Borics
Summary: Recent advancements in experimental structural biology have provided high-resolution structures of G protein-coupled receptors, shedding light on the mechanisms of transition between active and inactive states. Molecular dynamics simulations demonstrate that external stimulus transmission to the intracellular surface of the receptor involves subtle movements of polar amino acid side chains.
Article
Multidisciplinary Sciences
Rong-Chang Li, Laurie L. Molday, Chih-Chun Lin, Xiaozhi Ren, Alexander Fleischmann, Robert S. Molday, King-Wai Yau
Summary: This study provides evidence that the ligand-triggered GPCR pathway in mammalian olfactory transduction produces less than one downstream effector when an odorant-receptor molecule is complexed with its most effective odorants. This finding suggests a potential paradigm shift in our understanding of GPCR signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Yubo Cao, Wijnand J. C. van der Velden, Yoon Namkung, Anita K. Nivedha, Aaron Cho, Dana Sedki, Brian Holleran, Nicholas Lee, Richard Leduc, Sanychen Muk, Keith Le, Supriyo Bhattacharya, Nagarajan Vaidehi, Stephane A. Laporte
Summary: By studying the AT1R receptor through mutagenesis and molecular dynamics simulations, we found that G protein coupling involves multiple residues spread across the receptor, while beta-arrestin coupling is regulated by fewer structural regions. We also identified stretches of residues in transmembrane domain 4 that confer beta-arrestin bias. Additionally, allosteric communication within AT1R spreads beyond the orthosteric AngII-binding site. These findings provide insights into the structural elements and mechanisms underlying receptor coupling and offer potential for the design of biased receptors and allosteric modulators.
Review
Physiology
Tiantian Su, Qiuyun Guan, Huijuan Cheng, Zhenduo Zhu, Chunru Jiang, Paipai Guo, Yu Tai, Hanfei Sun, Manman Wang, Wei Wei, Qingtong Wang
Summary: GPR56 is a human G protein-coupled receptor that plays important roles in various physiological and pathological processes, including the development of the central nervous system, the development and differentiation of hematopoietic stem cells, and tumor metastasis. Its long N-terminal extracellular region makes it an attractive therapeutic target for neurological and hematopoietic diseases.
Article
Cell Biology
Jakob Mitgau, Julius Franke, Camilla Schinner, Gabriele Stephan, Sandra Berndt, Dimitris G. Placantonakis, Hermann Kalwa, Volker Spindler, Caroline Wilde, Ines Liebscher
Summary: This study provides information on the structural requirements and forces needed for ECM-mediated activation of the GPR126 receptor. The N terminus of GPR126 acts as an allosteric module that can fine-tune receptor activation in a context-specific manner.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Preeti Kumari Chaudhary, Soochong Kim
Summary: GPCRs play a crucial role in cancer progression, but the specific mechanisms of their involvement are still unclear. Studies have revealed the multifaceted impact of GPCRs on cancer development and the signaling pathways involved.
Article
Biochemistry & Molecular Biology
Xue Li, Qi Fu, Fu-Xing Zhao, Yi-Qing Wu, Teng-Yue Zhang, Zhong-Qi Li, Jun-Min He
Summary: This study found that heterotrimeric G proteins play a key role in UV-B and ethylene-induced stomatal closure in plants. GCR1 positively controls stomatal closure by activating GPA1-dependent ROS and NO production in guard cells. Ethylene acts upstream of GCR1 in transducing UV-B signaling in guard cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Reid H. J. Olsen, Justin G. English
Summary: Enzymatic and cellular signaling biosensors are powerful tools for understanding complex biological systems. G protein-coupled receptors (GPCRs) are extensively studied using biosensors, which have expanded our knowledge of this important class of proteins. Transducer biosensors that measure receptor coupling and selectivity, with a focus on receptor association and activation of heterotrimeric signaling complexes, are of particular importance.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ying Liu, Xiaoyun Wang, Danhui Dong, Luqin Guo, Xiaonan Dong, Jing Leng, Bing Zhao, Yang-Dong Guo, Na Zhang
Summary: G-proteins and GPCRs, as crucial signal transducers in signal transduction, have attracted increasing attention in both animal and plant research. The mode of action of G-proteins differs significantly between animals and plants, with plants possessing self-activated G alpha subunits and unique GPCR functions. More research is needed to understand the regulatory mechanisms and roles of uncanonical GPCRs in plant stress resistance and agricultural production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Li-Hua Zhao, Jingyu Lin, Su-Yu Ji, X. Edward Zhou, Chunyou Mao, Dan-Dan Shen, Xinheng He, Peng Xiao, Jinpeng Sun, Karsten Melcher, Yan Zhang, Xiao Yu, H. Eric Xu
Summary: This study presents the structures of CRF2R bound to UCN1 and coupled to G proteins G(11) and G(o), and compares them with the structure of CRF2R bound to G(s), uncovering the structural differences that determine the selective coupling of G protein subtypes by CRF2R.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Sabrina C. Brunetti, Michelle K. M. Arseneault, Justin A. Wright, Zhejun Wang, Mohammad-Reza Ehdaeivand, Michael J. Lowden, Jean Rivoal, Hala B. Khalil, Gajra Garg, Patrick J. Gulick
Summary: Heterotrimeric G proteins and calcium signaling both play roles in plant responses to environmental stress. The interaction between the calcium-binding protein RD20/CLO3 and the G-protein subunit GPA1 influences plant growth and development, affecting processes like hypocotyl length and leaf morphology. RD20/CLO3 acts as a negative regulator of GPA1, providing a novel insight into their functional relationship.
PLANT MOLECULAR BIOLOGY
(2021)
Review
Plant Sciences
Yijie Wang, Jose Ramon Botella
Summary: As sessile organisms, plants have evolved mechanisms to adapt to environmental fluctuations. Heterotrimeric G proteins play essential roles in the response to stress in plants, regulating multiple signaling pathways. Understanding the functions of G proteins in stress reactions can contribute to the development of resilient crops and global food security.
Review
Pharmacology & Pharmacy
Sergi Ferre, Francisco Ciruela, Carmen W. Dessauer, Javier Gonzalez-Maeso, Terence E. Hebert, Ralf Jockers, Diomedes E. Logothetis, Leonardo Pardo
Summary: The study proposes the concept of GPCR-effect assemblies (GEMMAs), which are pre-assembled before receptor activation and allow more efficient interactions between specific signaling components. This offers an alternative model to the conventional collision coupling model and explains the differential properties of GPCRs in different cellular environments.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Medicine, General & Internal
James Meyerhoff, Nabarun Chakraborty, Rasha Hammamieh
Summary: This article investigates the functionality and polarization of the glymphatic system under normal conditions, as well as the effects of traumatic brain injury and seizures. The study found that injury may disrupt the polarization of the glymphatic system, leading to brain edema. Additionally, sleep deprivation, which often occurs during military operations, could compromise glymphatic function. The study suggests further exploration of the effects of military threats on the glymphatic system and the proposal and evaluation of preventive and therapeutic measures.
Article
Environmental Sciences
Anastasia L. Yang, Nandula Raghuram, Tapan Kumar Adhya, Stephen D. Porter, Ananta Narayan Panda, Himadri Kaushik, Anuradha Jayaweera, Sarath Premalal Nissanka, Asif Reza Anik, Sharmin Shifa, Shaima Chowdhury Sharna, Rajendra Joshi, Muhammad Arif Watto, Anju Pokharel, Aminath Shazly, Rifaath Hassan, Sangeeta Bansal, David Kanter, Smriti Das, Roger Jeffery
Summary: Assessing and managing nitrogen sustainably is crucial for achieving the UN Sustainable Development Goals. This paper provides new insights into nitrogen-related policies in South Asia and emphasizes the need for more integrated approaches to address nitrogen pollution.
ENVIRONMENTAL RESEARCH LETTERS
(2022)
Article
Plant Sciences
Vikas Kumar Mandal, Annie Prasanna Jangam, Navjyoti Chakraborty, Nandula Raghuram
Summary: The research expands the repertoire of genes/processes involved in nitrate response in rice and reveals their physiological, phenotypic, and agronomic implications for nitrogen use efficiency (NUE).
Editorial Material
Environmental Sciences
Wilfried Winiwarter, Barbara Amon, Benjamin Leon Bodirsky, Henning Friege, Markus Geupel, Luis Lassaletta, Nandula Raghuram
Summary: This article collects scientific evidence on reactive nitrogen and the UN sustainable development goals, highlighting the relevance of agriculture as a key sector for nitrogen application and its impacts on the environment. The collection provides insight from field studies, laboratory research, and modeling approaches across multiple scales and continents, offering an overview of nitrogen science in the early 21st century. The evidence presented in this collection serves as a valuable basis for a global assessment of reactive nitrogen.
ENVIRONMENTAL RESEARCH LETTERS
(2022)
Article
Environmental Sciences
Sangeeta Bansal, Nandula Raghuram, Tapan Kumar Adhya, Md Mizanur Rahman, Dendup Tshering, Khem Raj Dahal, Abdul Wakeel, Shazly Aminath, Zikrullah Safi, Sarath Nissanka, Himanshu Pathak, Tariq Aziz, Umme Aminum Naher, Warshi Dandeniya, Jatish Chandra Biswas, Jitender Taneja, Ananta Narayan Panda, Himadri Kaushik, Niveta Jain, Ute Skiba, Ramesh Ramachandran, Mark A. Sutton
Summary: The N2O emissions in South Asia, particularly from industries, road transport, and power generation, have significantly increased. Although some measures have been taken to reduce emissions, more efforts are needed to address this issue.
ENVIRONMENTAL RESEARCH LETTERS
(2022)
Editorial Material
Plant Sciences
Nandula Raghuram, Tariq Aziz, Surya Kant, Jianbin Zhou, Susanne Schmidt
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Narendra Sharma, Supriya Kumari, Dinesh Kumar Jaiswal, Nandula Raghuram
Summary: This study investigates the transcriptomic analysis of nitrate response in two contrasting rice genotypes and reveals the processes/functions underlying differential nitrogen response and nitrogen use efficiency. The results show that the two genotypes have both common and unique gene expression patterns and functional categories in response to low nitrogen. The protein-protein interaction networks also differ between the two genotypes, with Nidhi showing involvement in cytoskeleton organization and cell wall processes, while Panvel1 showing involvement in chloroplast development. The study also identifies NUE genes and their differential distribution on the same chromosomes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Public, Environmental & Occupational Health
Meenakshi Sharma, Megha Brijwal, Nabarun Chakraborty, Aashish Choudhary, Arbind Kumar, Sharad Srivastav, Parin Lalwani, Richa Agrawal, Kapil Dev Soni, Nirupam Madaan, Rajesh Malhotra, Purva Mathur, Sanjeev Lalwani, Lalit Dar, Anjan Trikha
Summary: This study investigates the shedding rate of SARS-CoV-2 RNA from cadavers. The results suggest that the real-time RT-PCR positivity of SARS-CoV-2 RNA decreases with time.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Narendra Sharma, Dinesh Kumar Jaiswal, Supriya Kumari, Goutam Kumar Dash, Siddharth Panda, Annamalai Anandan, Nandula Raghuram
Summary: Rice is an ideal crop for improving nitrogen use efficiency (NUE), especially with urea fertilizer. However, there is limited research on contrasting rice genotypes for NUE. In this study, we compared the transcriptomes of low urea-responsive genotypes, Nidhi (low NUE) and Panvel1 (high NUE). Our findings showed differential expression of genes related to nutrient transporters, miRNAs, transcription factors, photosynthesis, water use efficiency, and post-translational modifications in high NUE rice. Furthermore, field evaluations demonstrated the superior agronomic performance of Panvel1, including grain yield and NUE. Our study also revealed common processes between urea-based and nitrate-based transcriptomes, suggesting the involvement of transporters and transcription factors in urea uptake and utilization for high NUE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Narendra Sharma, Bhumika Madan, M. Suhail Khan, Kuljeet S. Sandhu, Nandula Raghuram
Summary: In this study, 3020 common genes, referred to as N-responsive genes, were identified from 16 published N-responsive transcriptomes/microarrays. These genes include various transcription factors, transporters, miRNA targets, kinases, and post-translational modifications. Furthermore, 2298 out of the 3020 N-responsive genes contain G-quadruplex sequences. A comparison with previously identified NUE-related genes revealed that 17% of the NUE-related genes also contain G-quadruplex sequences. Six potential genes were identified as candidates to improve NUE based on network analysis and G4 sequence analysis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Green & Sustainable Science & Technology
Sandeep Gawdiya, Dinesh Kumar, Yashbir S. S. Shivay, Arti Bhatia, Shweta Mehrotra, Mandapelli Sharath Chandra, Anita Kumawat, Rajesh Kumar, Adam H. H. Price, Nandula Raghuram, Himanshu Pathak, Mark A. A. Sutton
Summary: This study aimed to screen rice genotypes with high nitrogen use efficiency under different nitrogen fertilization treatments. The results showed that applying the recommended amount of nitrogen yielded the highest panicles, filled grains panicle, leaf area index, tillers, grain yield, and harvest index. The N-60 and N-120 treatments had a higher effect compared to the N-0 treatment.
Article
Plant Sciences
Jangam Annie Prasanna, Vikas Kumar Mandal, Dinesh Kumar, Navjyoti Chakraborty, Nandula Raghuram
Summary: In this study, the knock-out mutant of RGA1 in rice was characterized to understand its role in nitrogen response and use efficiency. The results showed that RGA1 negatively regulates N-dose sensitivity and NUE in rice, and identified several novel RGA1-regulated genes/processes/traits related to nitrogen use efficiency.
PLANT CELL REPORTS
(2023)
