期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 78, 期 6, 页码 2683-2708出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-020-03725-2
关键词
CRISPR; Cas; Gene editing; Gene delivery; Gene therapy
资金
- National Health and Medical Research Council of Australia [1185600, 1123329]
- Ophthalmic Research Institute of Australia
- Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation [ZDSYS20190902093409851]
- National Health and Medical Research Council of Australia [1123329, 1185600] Funding Source: NHMRC
The CRISPR/Cas system is a groundbreaking genetic technology that allows gene modification by targeting specific genomic sites. Despite its versatility, the translation of CRISPR/Cas system still faces hurdles that need to be overcome, especially in improving delivery efficiency for potential applications in various fields.
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system provides a groundbreaking genetic technology that allows scientists to modify genes by targeting specific genomic sites. Due to the relative simplicity and versatility of the CRISPR/Cas system, it has been extensively applied in human genetic research as well as in agricultural applications, such as improving crops. Since the gene editing activity of the CRISPR/Cas system largely depends on the efficiency of introducing the system into cells or tissues, an efficient and specific delivery system is critical for applying CRISPR/Cas technology. However, there are still some hurdles remaining for the translatability of CRISPR/Cas system. In this review, we summarized the approaches used for the delivery of the CRISPR/Cas system in mammals, plants, and aquacultures. We further discussed the aspects of delivery that can be improved to elevate the potential for CRISPR/Cas translatability
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据