期刊
JOURNAL OF GENETICS AND GENOMICS
卷 46, 期 11, 页码 523-529出版社
SCIENCE PRESS
DOI: 10.1016/j.jgg.2019.11.003
关键词
CRISPR; Genome editing; Engineered guide RNA; Off-target effects
资金
- National Key Research and Development Program of China [2018YFA0801401, 2019YFA0802801]
- National Natural Science Foundation of China [31871345]
- Young Thousand Talented Program from Wuhan University
- Wuhan University
As versatile and robust genome editing tools, clustered regularly interspaced short palindromic repeats (CRISPR) technologies have been broadly used in basic research, biotechnology, and therapeutic development. Off-target mutagenesis by CRISPR systems has been demonstrated, and various methods have been developed to markedly increase their specificity. In this review, we highlight the efforts of producing and modifying guide RNA (gRNA) to minimize off-target activities, including sequence and structure design, tuning expression and chemical modification. The modalities of gRNA engineering can be applied across CRISPR systems. In conjunction with CRISPR protein effectors, the engineered gRNA enables efficient and precise genome editing. Copyright (C) 2019, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据