The number of catalytic cycles in an enzyme’s lifetime and why it matters to metabolic engineering

Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s

(2020) Jaya Joshi et al.   BIOCHEMICAL JOURNAL

Enzymes as Parts in Need of Replacement – and How to Extend Their Working Life

(2020) Nathan D. Tivendale et al.   TRENDS IN PLANT SCIENCE

Characterization of maximal enzyme catalytic rates in central metabolism of Arabidopsis thaliana

(2020) Anika Küken et al.   PLANT JOURNAL

Engineering Strategies to Boost Crop Productivity by Cutting Respiratory Carbon Loss

(2019) Jeffrey S Amthor et al.   PLANT CELL

The Scope, Functions, and Dynamics of Posttranslational Protein Modifications

(2019) A. Harvey Millar et al.   Annual Review of Plant Biology

The importance of catalytic promiscuity for enzyme design and evolution

(2019) Reuben B. Leveson-Gower et al.   Nature Reviews Chemistry

Regulated Proteolysis in Bacteria

(2018) Samar A. Mahmoud et al.   Annual Review of Biochemistry

Replacement of oxidizable residues predicted by QM-MM simulation of a fungal laccase generates variants with higher operational stability

(2018) Mayra Avelar et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Redesigning thiamin synthesis: Prospects and potential payoffs

(2018) Andrew D. Hanson et al.   PLANT SCIENCE

‘Negative’ and ‘positive catalysis’: complementary principles that shape the catalytic landscape of enzymes

(2018) Bastian Vögeli et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Parts-prospecting For a High-efficiency Thiamin Thiazole Biosynthesis Pathway

(2018) Jiayi Sun et al.   PLANT PHYSIOLOGY

Non-enzymatic molecular damage as a prototypic driver of aging

(2017) Alexey Golubev et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Probing the acetaldehyde-sensitivity of 2-deoxy-ribose-5-phosphate aldolase (DERA) leads to resistant variants

(2017) Julia Bramski et al.   JOURNAL OF BIOTECHNOLOGY

Protein Degradation Rate in Arabidopsis thaliana Leaf Growth and Development

(2017) Lei Li et al.   PLANT CELL

Growth rate correlates negatively with protein turnover in Arabidopsis accessions

(2017) Hirofumi Ishihara et al.   PLANT JOURNAL

Critical structural fluctuations of proteins upon thermal unfolding challenge the Lindemann criterion

(2017) Marina Katava et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Determinants and Regulation of Protein Turnover in Yeast

(2017) Miguel Martin-Perez et al.   Cell Systems

'Nothing of chemistry disappears in biology: the Top 30 damage-prone endogenous metabolites

(2016) C. Lerma-Ortiz et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Opportunities for wheat proteomics to discover the biomarkers for respiration-dependent biomass production, stress tolerance and cytoplasmic male sterility

(2016) Richard P. Jacoby et al.   Journal of Proteomics

BRENDA in 2017: new perspectives and new tools in BRENDA

(2016) Sandra Placzek et al.   NUCLEIC ACIDS RESEARCH

Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitrokcatmeasurements

(2016) Dan Davidi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanism-based inhibition of an aldolase at high concentrations of its natural substrate acetaldehyde: structural insights and protective strategies

(2016) Markus Dick et al.   Chemical Science

The Moderately Efficient Enzyme: Futile Encounters and Enzyme Floppiness

(2015) Arren Bar-Even et al.   BIOCHEMISTRY

Hole hopping through tyrosine/tryptophan chains protects proteins from oxidative damage

(2015) Harry B. Gray et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Version 4.0 of PaxDb: Protein abundance data, integrated across model organisms, tissues, and cell-lines

(2015) Mingcong Wang et al.   PROTEOMICS

Quality Control and Infiltration of Translation by Amino Acids Outside of the Genetic Code

(2014) Tammy Bullwinkle et al.   Annual Review of Genetics

Integrating Biological Redesign: Where Synthetic Biology Came From and Where It Needs to Go

(2014) Jeffrey C. Way et al.   CELL

Accuracy-rate tradeoffs: how do enzymes meet demands of selectivity and catalytic efficiency?

(2014) Dan S Tawfik   CURRENT OPINION IN CHEMICAL BIOLOGY

Protein turnover forms one of the highest maintenance costs in Lactococcus lactis

(2014) P.-J. Lahtvee et al.   MICROBIOLOGY-SGM

Engineering explored

(2014) Pamela A. Silver et al.   NATURE

Realizing the potential of synthetic biology

(2014) George M. Church et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Isotopically nonstationary13C flux analysis of changes inArabidopsis thalianaleaf metabolism due to high light acclimation

(2014) Fangfang Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-scale metabolic model for Lactococcus lactis MG1363 and its application to the analysis of flavor formation

(2013) Nicolas A. L. Flahaut et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Mass Spectrometry of Membrane Proteins: A Focus on Aquaporins

(2013) Kevin L. Schey et al.   BIOCHEMISTRY

Tuning the dials of Synthetic Biology

(2013) J. A. J. Arpino et al.   MICROBIOLOGY-SGM

Escherichia coli achieves faster growth by increasing catalytic and translation rates of proteins

(2013) Kaspar Valgepea et al.   Molecular BioSystems

Dynamic13C-labeling experiments prove important differences in protein turnover rate between twoSaccharomyces cerevisiaestrains

(2012) Kuk-Ki Hong et al.   FEMS YEAST RESEARCH

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

“Turning Over” Definitions in Catalytic Cycles

(2012) Sebastian Kozuch et al.   ACS Catalysis

The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters

(2011) Arren Bar-Even et al.   BIOCHEMISTRY

Improving the iMM904 S. cerevisiae metabolic model using essentiality and synthetic lethality data

(2011) Ali R Zomorrodi et al.   BMC Systems Biology

Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase

(2011) Abhishek Chatterjee et al.   NATURE

High-throughput generation, optimization and analysis of genome-scale metabolic models

(2010) Christopher S Henry et al.   NATURE BIOTECHNOLOGY

Connecting extracellular metabolomic measurements to intracellular flux states in yeast

(2009) Monica L Mo et al.   BMC Systems Biology

Utilizing simple biochemical measurements to predict lifetime output of biocatalysts in continuous isothermal processes

(2009) Thomas A. Rogers et al.   CHEMICAL ENGINEERING SCIENCE

Activity-Based Protein Profiling: New Developments and Directions in Functional Proteomics

(2008) Mahesh Uttamchandani et al.   CHEMBIOCHEM