Ubiquitin ligation to F-box protein targets by SCF–RBR E3–E3 super-assembly

NEDD8 nucleates a multivalent cullin–RING–UBE2D ubiquitin ligation assembly

(2020) Kheewoong Baek et al.   NATURE

ARIH2 Is a Vif-Dependent Regulator of CUL5-Mediated APOBEC3G Degradation in HIV Infection

(2019) Ruth Hüttenhain et al.   Cell Host & Microbe

Robust cullin-RING ligase function is established by a multiplicity of poly-ubiquitylation pathways

(2019) Spencer Hill et al.   eLife

Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression

(2018) Xing Liu et al.   MOLECULAR CELL

Mechanism of parkin activation by PINK1

(2018) Christina Gladkova et al.   NATURE

Mechanism of parkin activation by phosphorylation

(2018) Véronique Sauvé et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanism of parkin activation by phosphorylation

(2018) Véronique Sauvé et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

New tools for the analysis and validation of cryo-EM maps and atomic models

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

Synergistic recruitment of UbcH7~Ub and phosphorylated Ubl domain triggers parkin activation

(2018) Tara EC Condos et al.   EMBO JOURNAL

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

Activity-based probes for the ubiquitin conjugation-deconjugation machinery: new chemistries, new tools, and new insights

(2017) David S. Hewings et al.   FEBS Journal

Parkin–phosphoubiquitin complex reveals cryptic ubiquitin-binding site required for RBR ligase activity

(2017) Atul Kumar et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Two functionally distinct E2/E3 pairs coordinate sequential ubiquitination of a common substrate inCaenorhabditis elegansdevelopment

(2017) Katja K. Dove et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Studies of HHARI/UbcH7∼Ub Reveal Unique E2∼Ub Conformational Restriction by RBR RING1

(2017) Katja K. Dove et al.   STRUCTURE

Parkin–phosphoubiquitin complex reveals cryptic ubiquitin-binding site required for RBR ligase activity

(2017) Atul Kumar et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation

(2016) Daniel C. Scott et al.   CELL

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation

(2016) Bernhard C. Lechtenberg et al.   NATURE

A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes

(2016) Monique P C Mulder et al.   Nature Chemical Biology

High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities

(2015) Traver Hart et al.   CELL

Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis

(2015) A. Kumar et al.   EMBO JOURNAL

Mechanism of phospho-ubiquitin-induced PARKIN activation

(2015) Tobias Wauer et al.   NATURE

Identification and characterization of essential genes in the human genome

(2015) T. Wang et al.   SCIENCE

Structure of a RING E3 Trapped in Action Reveals Ligation Mechanism for the Ubiquitin-like Protein NEDD8

(2014) Daniel C. Scott et al.   CELL

A Native Chemical Ligation Handle that Enables the Synthesis of Advanced Activity-Based Probes: Diubiquitin as a Case Study

(2014) Monique P. C. Mulder et al.   CHEMBIOCHEM

RING-type E3 ligases: Master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination

(2013) Meredith B. Metzger et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Cand1 Promotes Assembly of New SCF Complexes through Dynamic Exchange of F Box Proteins

(2013) Nathan W. Pierce et al.   CELL

Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes

(2013) Guorui Li et al.   CHEMICAL COMMUNICATIONS

TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes

(2013) Ian R Kelsall et al.   EMBO JOURNAL

Structure of the human Parkin ligase domain in an autoinhibited state

(2013) Tobias Wauer et al.   EMBO JOURNAL

Building and remodelling Cullin-RING E3 ubiquitin ligases

(2013) John R Lydeard et al.   EMBO REPORTS

SCFFBXL3 ubiquitin ligase targets cryptochromes at their cofactor pocket

(2013) Weiman Xing et al.   NATURE

Structural basis for ligase-specific conjugation of linear ubiquitin chains by HOIP

(2013) Benjamin Stieglitz et al.   NATURE

Structure of Parkin Reveals Mechanisms for Ubiquitin Ligase Activation

(2013) J.-F. Trempe et al.   SCIENCE

Structure of HHARI, a RING-IBR-RING Ubiquitin Ligase: Autoinhibition of an Ariadne-Family E3 and Insights into Ligation Mechanism

(2013) David M. Duda et al.   STRUCTURE

CSN- and CAND1-dependent remodelling of the budding yeast SCF complex

(2013) Aleksandra Zemla et al.   Nature Communications

Structure and function of Parkin E3 ubiquitin ligase reveals aspects of RING and HECT ligases

(2013) B.E. Riley et al.   Nature Communications

Ubiquitin-Based Probes Prepared by Total Synthesis To Profile the Activity of Deubiquitinating Enzymes

(2012) Annemieke de Jong et al.   CHEMBIOCHEM

Selective Recruitment of an E2∼Ubiquitin Complex by an E3 Ubiquitin Ligase

(2012) Donald E. Spratt et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Deconjugation of Nedd8 from Cul1 Is Directly Regulated by Skp1-F-box and Substrate, and the COP9 Signalosome Inhibits Deneddylated SCF by a Noncatalytic Mechanism

(2012) Ethan D. Emberley et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of a Glomulin-RBX1-CUL1 Complex: Inhibition of a RING E3 Ligase through Masking of Its E2-Binding Surface

(2012) David M. Duda et al.   MOLECULAR CELL

Structure of an E3:E2∼Ub Complex Reveals an Allosteric Mechanism Shared among RING/U-box Ligases

(2012) Jonathan N. Pruneda et al.   MOLECULAR CELL

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis

(2012) Anna Plechanovová et al.   NATURE

BIRC7–E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural Basis for a Reciprocal Regulation between SCF and CSN

(2012) Radoslav I. Enchev et al.   Cell Reports

The Molecular Basis of CRL4DDB2/CSA Ubiquitin Ligase Architecture, Targeting, and Activation

(2011) Eric S. Fischer et al.   CELL

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids

(2011) Dawn M. Wenzel et al.   NATURE

Methods for converting cysteine to dehydroalanine on peptides and proteins

(2011) Justin M. Chalker et al.   Chemical Science

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Priming and Extending: A UbcH5/Cdc34 E2 Handoff Mechanism for Polyubiquitination on a SCF Substrate

(2010) Kenneth Wu et al.   MOLECULAR CELL

Detection of sequential polyubiquitylation on a millisecond timescale

(2009) Nathan W. Pierce et al.   NATURE

An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer

(2009) Teresa A. Soucy et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation

(2008) David M. Duda et al.   CELL

Multimodal Activation of the Ubiquitin Ligase SCF by Nedd8 Conjugation

(2008) Anjanabha Saha et al.   MOLECULAR CELL