A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes

Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

(2016) Heeseon An et al.   CHEMICAL COMMUNICATIONS

A Stable Chemical SUMO1-Ubc9 Conjugate Specifically Binds as a Thioester Mimic to the RanBP2-E3 Ligase Complex

(2015) Stefanie Sommer et al.   CHEMBIOCHEM

Structure of a RING E3 Trapped in Action Reveals Ligation Mechanism for the Ubiquitin-like Protein NEDD8

(2014) Daniel C. Scott et al.   CELL

Catching a DUB in the act: novel ubiquitin-based active site directed probes

(2014) Reggy Ekkebus et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Characterization of Ubiquitin-Activating Enzyme Uba1 in the Nucleus by Its Mammalian Temperature-Sensitive Mutant

(2014) Kimihiko Sugaya et al.   PLoS One

Structure of the ubiquitin-activating enzyme loaded with two ubiquitin molecules

(2014) Antje Schäfer et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated TNFα production

(2013) Manoj B. Menon et al.   BIOCHEMICAL JOURNAL

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L

(2013) R. van der Kant et al.   JOURNAL OF CELL SCIENCE

Exploring a New Frontier in Cancer Treatment: Targeting the Ubiquitin and Ubiquitin-like Activating Enzymes

(2013) Sara R. da Silva et al.   JOURNAL OF MEDICINAL CHEMISTRY

Development of Activity-Based Probes for Ubiquitin and Ubiquitin-like Protein Signaling Pathways

(2013) Heeseon An et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases

(2013) Reggy Ekkebus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structure of a Ubiquitin E1-E2 Complex: Insights to E1-E2 Thioester Transfer

(2013) Shaun K. Olsen et al.   MOLECULAR CELL

The Ubiquitin Code

(2012) David Komander et al.   Annual Review of Biochemistry

Structural Insights into the Conformation and Oligomerization of E2∼Ubiquitin Conjugates

(2012) Richard C. Page et al.   BIOCHEMISTRY

Detection of ubiquitin–proteasome enzymatic activities in cells: Application of activity-based probes to inhibitor development

(2012) Holger B. Kramer et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

(2012) Kyle J. Roux et al.   JOURNAL OF CELL BIOLOGY

Structure of an E3:E2∼Ub Complex Reveals an Allosteric Mechanism Shared among RING/U-box Ligases

(2012) Jonathan N. Pruneda et al.   MOLECULAR CELL

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis

(2012) Anna Plechanovová et al.   NATURE

The mechanism of OTUB1-mediated inhibition of ubiquitination

(2012) Reuven Wiener et al.   NATURE

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Ubiquitin in Motion: Structural Studies of the Ubiquitin-Conjugating Enzyme∼Ubiquitin Conjugate

(2011) Jonathan N. Pruneda et al.   BIOCHEMISTRY

Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment

(2011) Jürgen Cox et al.   JOURNAL OF PROTEOME RESEARCH

The Phenix software for automated determination of macromolecular structures

(2011) Paul D. Adams et al.   METHODS

Methods for converting cysteine to dehydroalanine on peptides and proteins

(2011) Justin M. Chalker et al.   Chemical Science

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin

(2010) Farid El Oualid et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Recovering lost magnetization: polarization enhancement in biomolecular NMR

(2010) Adrien Favier et al.   JOURNAL OF BIOMOLECULAR NMR

Designed Semisynthetic Protein Inhibitors of Ub/Ubl E1 Activating Enzymes

(2010) Xuequan Lu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Active site remodelling accompanies thioester bond formation in the SUMO E1

(2010) Shaun K. Olsen et al.   NATURE

Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1

(2010) Shinichiro Nakada et al.   NATURE

Exploitation of the Ubiquitin System by Invading Bacteria

(2010) Olivia Steele-Mortimer   TRAFFIC

The family of ubiquitin-conjugating enzymes (E2s): deciding between life and death of proteins

(2009) Sjoerd J. L. van Wijk et al.   FASEB JOURNAL

Insights into Ubiquitin Transfer Cascades from a Structure of a UbcH5B∼Ubiquitin-HECTNEDD4L Complex

(2009) Hari B. Kamadurai et al.   MOLECULAR CELL

Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways

(2009) Brenda A. Schulman et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Physiological functions of the HECT family of ubiquitin ligases

(2009) Daniela Rotin et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Activity-Based Protein Profiling: From Enzyme Chemistry to Proteomic Chemistry

(2008) Benjamin F. Cravatt et al.   Annual Review of Biochemistry

Final Stages of Cytokinesis and Midbody Ring Formation Are Controlled by BRUCE

(2008) Christian Pohl et al.   CELL

Facile Conversion of Cysteine and Alkyl Cysteines to Dehydroalanine on Protein Surfaces: Versatile and Switchable Access to Functionalized Proteins

(2008) Gonçalo J. L. Bernardes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

(2008) Jürgen Cox et al.   NATURE BIOTECHNOLOGY