Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization

Mechanism of ribosome shutdown by RsfS in Staphylococcus aureus revealed by integrative structural biology approach

(2020) Iskander Khusainov et al.   Nature Communications

Loss of a single methylation in 23S rRNA delays 50S assembly at multiple late stages and impairs translation initiation and elongation

(2020) Wei Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly

(2020) Shijie Huang et al.   Nature Communications

Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome

(2019) Yi Zhou et al.   Nature Communications

Mechanism of completion of peptidyltransferase centre assembly in eukaryotes

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Structural consequences of the interaction of RbgA with a 50S ribosomal subunit assembly intermediate

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Structural Visualization of the Formation and Activation of the 50S Ribosomal Subunit during In Vitro Reconstitution

(2018) Rainer Nikolay et al.   MOLECULAR CELL

Modular assembly of the nucleolar pre-60S ribosomal subunit

(2018) Zahra Assur Sanghai et al.   NATURE

cisTEM, user-friendly software for single-particle image processing

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Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors

(2018) Daichi Tamaru et al.   RNA

UniProt: a worldwide hub of protein knowledge

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eggNOG 5.0: a hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses

(2018) Jaime Huerta-Cepas et al.   NUCLEIC ACIDS RESEARCH

tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis

(2018) Julia Flis et al.   Cell Reports

Structural and biochemical analysis ofEscherichia coliObgE, a central regulator of bacterial persistence

(2017) Sotirios Gkekas et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Ensemble cryo-EM elucidates the mechanism of translation fidelity

(2017) Anna B. Loveland et al.   NATURE

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Structures of the human mitochondrial ribosome in native states of assembly

(2017) Alan Brown et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

A Puzzle of Life: Crafting Ribosomal Subunits

(2017) Dieter Kressler et al.   TRENDS IN BIOCHEMICAL SCIENCES

Recurring RNA structural motifs underlie the mechanics of L1 stalk movement

(2017) Srividya Mohan et al.   Nature Communications

Structures of the human mitochondrial ribosome in native states of assembly

(2017) Alan Brown et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Role of protein L25 and its contact with protein L16 in maintaining the active state of Escherichia coli ribosomes in vivo

(2016) A. Y. Anikaev et al.   BIOCHEMISTRY-MOSCOW

Modular Assembly of the Bacterial Large Ribosomal Subunit

(2016) Joseph H. Davis et al.   CELL

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Diverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomes

(2016) Shan Wu et al.   NATURE

YphC and YsxC GTPases assist the maturation of the central protuberance, GTPase associated region and functional core of the 50S ribosomal subunit

(2016) Xiaodan Ni et al.   NUCLEIC ACIDS RESEARCH

The impact of recent improvements in cryo-electron microscopy technology on the understanding of bacterial ribosome assembly

(2016) Aida Razi et al.   NUCLEIC ACIDS RESEARCH

70S-scanning initiation is a novel and frequent initiation mode of ribosomal translation in bacteria

(2016) Hiroshi Yamamoto et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

(2016) Dari Kimanius et al.   eLife

Fluorescence-based monitoring of ribosome assembly landscapes

(2015) Rainer Nikolay et al.   BMC MOLECULAR BIOLOGY

Obg and Membrane Depolarization Are Part of a Microbial Bet-Hedging Strategy that Leads to Antibiotic Tolerance

(2015) Natalie Verstraeten et al.   MOLECULAR CELL

Single methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly

(2015) Taiga Arai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of Ribosomal Silencing Factor Bound to Mycobacterium tuberculosis Ribosome

(2015) Xiaojun Li et al.   STRUCTURE

Activation of GTP hydrolysis in mRNA-tRNA translocation by elongation factor G

(2015) W. Li et al.   Science Advances

Regulation of the Mammalian Elongation Cycle by Subunit Rolling: A Eukaryotic-Specific Ribosome Rearrangement

(2014) Tatyana V. Budkevich et al.   CELL

Quantitative Analysis of rRNA Modifications Using Stable Isotope Labeling and Mass Spectrometry

(2014) Anna M. Popova et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Validation of a fluorescence-based screening concept to identify ribosome assembly defects inEscherichia coli

(2014) Rainer Nikolay et al.   NUCLEIC ACIDS RESEARCH

Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly

(2014) Xiaoxiao Zhang et al.   NUCLEIC ACIDS RESEARCH

Structural and Functional Insights into the Mode of Action of a Universally Conserved Obg GTPase

(2014) Boya Feng et al.   PLOS BIOLOGY

Bacterial Obg proteins: GTPases at the nexus of protein and DNA synthesis

(2013) Cyrielle Kint et al.   CRITICAL REVIEWS IN MICROBIOLOGY

Functional domains of the 50S subunit mature late in the assembly process

(2013) A. Jomaa et al.   NUCLEIC ACIDS RESEARCH

Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit

(2013) N. Li et al.   NUCLEIC ACIDS RESEARCH

Crystal Structures of EF-G-Ribosome Complexes Trapped in Intermediate States of Translocation

(2013) J. Zhou et al.   SCIENCE

Control of Ribosomal Subunit Rotation by Elongation Factor G

(2013) A. Pulk et al.   SCIENCE

Elongation Factor G Bound to the Ribosome in an Intermediate State of Translocation

(2013) D. S. Tourigny et al.   SCIENCE

High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy

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Gateway Role for rRNA Precursors in Ribosome Assembly

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Biochemical Characterization of Ribosome Assembly GTPase RbgA inBacillus subtilis

(2012) David Achila et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Characterization of the Ribosome Biogenesis Landscape in E. coli Using Quantitative Mass Spectrometry

(2012) Stephen S. Chen et al.   JOURNAL OF MOLECULAR BIOLOGY

Measuring the dynamics of E. coli ribosome biogenesis using pulse-labeling and quantitative mass spectrometry

(2012) Stephen S. Chen et al.   Molecular BioSystems

Protein L5 is crucial for in vivo assembly of the bacterial 50S ribosomal subunit central protuberance

(2012) Alexey P. Korepanov et al.   NUCLEIC ACIDS RESEARCH

RsfA (YbeB) Proteins Are Conserved Ribosomal Silencing Factors

(2012) Roman Häuser et al.   PLoS Genetics

Assembly of Bacterial Ribosomes

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The structure of helix 89 of 23S rRNA is important for peptidyl transferase function ofEscherichia coliribosome

(2011) Dmitry E. Burakovsky et al.   FEBS LETTERS

Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment

(2011) Jürgen Cox et al.   JOURNAL OF PROTEOME RESEARCH

The Universally Conserved Prokaryotic GTPases

(2011) N. Verstraeten et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Excited states of ribosome translocation revealed through integrative molecular modeling

(2011) P. C. Whitford et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of the Bacterial Ribosome in Classical and Hybrid States of tRNA Binding

(2011) J. A. Dunkle et al.   SCIENCE

Crystal Structure of the Eukaryotic 60S Ribosomal Subunit in Complex with Initiation Factor 6

(2011) S. Klinge et al.   SCIENCE

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Subribosomal particle analysis reveals the stages of bacterial ribosome assembly at which rRNA nucleotides are modified

(2010) T. Siibak et al.   RNA

The Mechanism for Activation of GTP Hydrolysis on the Ribosome

(2010) R. M. Voorhees et al.   SCIENCE

GTPase activation of elongation factor EF-Tu by the ribosome during decoding

(2009) Jan-Christian Schuette et al.   EMBO JOURNAL

Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli

(2009) Nobutaka Nakashima et al.   NUCLEIC ACIDS RESEARCH

Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis

(2009) E. Villa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

(2008) Jürgen Cox et al.   NATURE BIOTECHNOLOGY

The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit

(2008) L. Lancaster et al.   RNA