NMR spectroscopy captures the essential role of dynamics in regulating biomolecular function

Side Chain Hydrogen-Bonding Interactions within Amyloid-like Fibrils Formed by the Low-Complexity Domain of FUS: Evidence from Solid State Nuclear Magnetic Resonance Spectroscopy

(2020) Dylan T. Murray et al.   BIOCHEMISTRY

Abrogation of prenucleation, transient oligomerization of the Huntingtin exon 1 protein by human profilin I

(2020) Alberto Ceccon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A 300-fold enhancement of imino nucleic acid resonances by hyperpolarized water provides a new window for probing RNA refolding by 1D and 2D NMR

(2020) Mihajlo Novakovic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

(2020) Alexander E. Conicella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR

(2020) Or Szekely et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Base-pair conformational switch modulates miR-34a targeting of Sirt1 mRNA

(2020) Lorenzo Baronti et al.   NATURE

A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle

(2020) Gili Abramov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Automated assignment of methyl NMR spectra from large proteins

(2020) Iva Pritišanac et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Concentration‐dependent structural transition of the HIV‐1 gp41 MPER peptide into α‐helical trimers

(2020) Ad Bax et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Conformational states dynamically populated by a kinase determine its function

(2020) Tao Xie et al.   SCIENCE

Conformational landscape alternations promote oncogenic activities of Ras-related C3 botulinum toxin substrate 1 as revealed by NMR

(2019) Yuki Toyama et al.   Science Advances

Local unfolding of the HSP27 monomer regulates chaperone activity

(2019) T. Reid Alderson et al.   Nature Communications

Probing initial transient oligomerization events facilitating Huntingtin fibril nucleation at atomic resolution by relaxation-based NMR

(2019) Samuel A. Kotler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics

(2019) Andras Boeszoermenyi et al.   NATURE METHODS

An NMR View of Protein Dynamics in Health and Disease

(2019) Ashok Sekhar et al.   Annual Review of Biophysics

High-sensitivity protein solid-state NMR spectroscopy

(2019) Venkata S Mandala et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Characterising side chains in large proteins by protonless 13C-detected NMR spectroscopy

(2019) Ruth B. Pritchard et al.   Nature Communications

Using Deep Neural Networks to Reconstruct Non-uniformly Sampled NMR Spectra

(2019) D. Flemming Hansen   JOURNAL OF BIOMOLECULAR NMR

Protein NMR: Boundless opportunities

(2019) Ad Bax et al.   JOURNAL OF MAGNETIC RESONANCE

The roles of structural dynamics in the cellular functions of RNAs

(2019) Laura R. Ganser et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The Hsp70 chaperone network

(2019) Rina Rosenzweig et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid–Liquid Phase Separation in Disease

(2019) Simon Alberti et al.   Annual Review of Genetics

Observation of β-Amyloid Peptide Oligomerization by Pressure-Jump NMR Spectroscopy

(2019) C. Ashley Barnes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Application of millisecond time-resolved solid state NMR to the kinetics and mechanism of melittin self-assembly

(2019) Jaekyun Jeon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems

(2019) Stefan Schütz et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Structural basis for client recognition and activity of Hsp40 chaperones

(2019) Yajun Jiang et al.   SCIENCE

Interplay of disordered and ordered regions of a human small heat shock protein yields an ensemble of 'quasi-ordered' states

(2019) Amanda F Clouser et al.   eLife

Structural mapping of oligomeric intermediates in an amyloid assembly pathway

(2019) Theodoros K Karamanos et al.   eLife

Decreased conformational stability in the oncogenic N92I mutant of Ras-related C3 botulinum toxin substrate 1

(2019) Yuki Toyama et al.   Science Advances

Characterizing Post-Translational Modifications and Their Effects on Protein Conformation Using NMR Spectroscopy

(2019) Ajith Kumar et al.   BIOCHEMISTRY

Perspective: the essential role of NMR in the discovery and characterization of intrinsically disordered proteins

(2019) H. Jane Dyson et al.   JOURNAL OF BIOMOLECULAR NMR

The Ambivalent Role of Proline Residues in an Intrinsically Disordered Protein: From Disorder Promoters to Compaction Facilitators

(2019) Borja Mateos et al.   JOURNAL OF MOLECULAR BIOLOGY

Unraveling the structure and dynamics of the human DNAJB6b chaperone by NMR reveals insights into Hsp40-mediated proteostasis

(2019) Theodoros K. Karamanos et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Atomic-level insight into mRNA processing bodies by combining solid and solution-state NMR spectroscopy

(2019) Reinier Damman et al.   Nature Communications

NPM1 exhibits structural and dynamic heterogeneity upon phase separation with the p14ARF tumor suppressor

(2019) Eric Gibbs et al.   JOURNAL OF MAGNETIC RESONANCE

Protons as Versatile Reporters in Solid-State NMR Spectroscopy

(2018) Suresh K. Vasa et al.   ACCOUNTS OF CHEMICAL RESEARCH

A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing

(2018) Ailin Wang et al.   EMBO JOURNAL

Interaction of Huntingtin Exon-1 Peptides with Lipid-Based Micellar Nanoparticles Probed by Solution NMR and Q-Band Pulsed EPR

(2018) Alberto Ceccon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Probing Conformational Exchange in Weakly Interacting, Slowly Exchanging Protein Systems via Off-Resonance R1ρ Experiments: Application to Studies of Protein Phase Separation

(2018) Tairan Yuwen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Deciphering the Dynamic Interaction Profile of an Intrinsically Disordered Protein by NMR Exchange Spectroscopy

(2018) Elise Delaforge et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Molecular Mechanism of J-Domain-Triggered ATP Hydrolysis by Hsp70 Chaperones

(2018) Roman Kityk et al.   MOLECULAR CELL

Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation

(2018) Veronica H. Ryan et al.   MOLECULAR CELL

Cotranslocational processing of the protein substrate calmodulin by an AAA+ unfoldase occurs via unfolding and refolding intermediates

(2018) Rafal Augustyniak et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Effects of maturation on the conformational free-energy landscape of SOD1

(2018) Robert M. Culik et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Study of protein folding under native conditions by rapidly switching the hydrostatic pressure inside an NMR sample cell

(2018) Cyril Charlier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conserved conformational selection mechanism of Hsp70 chaperone-substrate interactions

(2018) Ashok Sekhar et al.   eLife

Oligomerization of a molecular chaperone modulates its activity

(2018) Tomohide Saio et al.   eLife

Dynamic basis for dG•dT misincorporation via tautomerization and ionization

(2018) Isaac J. Kimsey et al.   NATURE

Characterization of intrinsically disordered proteins and their dynamic complexes: From in vitro to cell-like environments

(2018) Sigrid Milles et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space

(2018) Katharina Weinhäupl et al.   CELL

Cooperative subunit dynamics modulate p97 function

(2018) Rui Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Probing the mechanism of inhibition of amyloid-β(1–42)–induced neurotoxicity by the chaperonin GroEL

(2018) Marielle A. Wälti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Probing conformational dynamics in biomolecules via chemical exchange saturation transfer: a primer

(2017) Pramodh Vallurupalli et al.   JOURNAL OF BIOMOLECULAR NMR

Structure and Dynamics of an Intrinsically Disordered Protein Region That Partially Folds upon Binding by Chemical-Exchange NMR

(2017) Cyril Charlier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An excited state underlies gene regulation of a transcriptional riboswitch

(2017) Bo Zhao et al.   Nature Chemical Biology

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Atomic view of the energy landscape in the allosteric regulation of Abl kinase

(2017) Tamjeed Saleh et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Exploiting conformational plasticity in the AAA+ protein VCP/p97 to modify function

(2017) Anne Kathrin Schütz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct observation of structure and dynamics during phase separation of an elastomeric protein

(2017) Sean E. Reichheld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

(2017) Jacob P. Brady et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformational heterogeneity in the Hsp70 chaperone-substrate ensemble identified from analysis of NMR-detected titration data

(2017) Ashok Sekhar et al.   PROTEIN SCIENCE

The dynamic dimer structure of the chaperone Trigger Factor

(2017) Leonor Morgado et al.   Nature Communications

Atomic view of the energy landscape in the allosteric regulation of Abl kinase

(2017) Tamjeed Saleh et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Promiscuous binding by Hsp70 results in conformational heterogeneity and fuzzy chaperone-substrate ensembles

(2017) Rina Rosenzweig et al.   eLife

Structure of a AAA+ unfoldase in the process of unfolding substrate

(2017) Zev A Ripstein et al.   eLife

Monomeric Aβ1–40 and Aβ1–42 Peptides in Solution Adopt Very Similar Ramachandran Map Distributions That Closely Resemble Random Coil

(2016) Julien Roche et al.   BIOCHEMISTRY

Sparse multidimensional iterative lineshape-enhanced (SMILE) reconstruction of both non-uniformly sampled and conventional NMR data

(2016) Jinfa Ying et al.   JOURNAL OF BIOMOLECULAR NMR

Capturing a Dynamic Chaperone–Substrate Interaction Using NMR-Informed Molecular Modeling

(2016) Loïc Salmon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural disorder of monomeric α-synuclein persists in mammalian cells

(2016) Francois-Xavier Theillet et al.   NATURE

Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study

(2016) Rui Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hsp70 biases the folding pathways of client proteins

(2016) Ashok Sekhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Huntingtin exon 1 fibrils feature an interdigitated β-hairpin–based polyglutamine core

(2016) Cody L. Hoop et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Probing the free energy landscapes of ALS disease mutants of SOD1 by NMR spectroscopy

(2016) Ashok Sekhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Studying dynamics by magic-angle spinning solid-state NMR spectroscopy: Principles and applications to biomolecules

(2016) Paul Schanda et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

In vivo aspects of protein folding and quality control

(2016) D. Balchin et al.   SCIENCE

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

A Dynamic molecular basis for malfunction in disease mutants of p97/VCP

(2016) Anne K Schuetz et al.   eLife

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Methyl-specific isotopic labeling: a molecular tool box for solution NMR studies of large proteins

(2015) Rime Kerfah et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Visualizing transient Watson–Crick-like mispairs in DNA and RNA duplexes

(2015) Isaac J. Kimsey et al.   NATURE

Intrinsically disordered proteins in cellular signalling and regulation

(2015) Peter E. Wright et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Heterogeneous binding of the SH3 client protein to the DnaK molecular chaperone

(2015) Jung Ho Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mapping the conformation of a client protein through the Hsp70 functional cycle

(2015) Ashok Sekhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Altered cofactor regulation with disease-associated p97/VCP mutations

(2015) Xiaoyi Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Visualizing transient dark states by NMR spectroscopy

(2015) Nicholas J. Anthis et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Thermal fluctuations of immature SOD1 lead to separate folding and misfolding pathways

(2015) Ashok Sekhar et al.   eLife

Hsp90-Tau Complex Reveals Molecular Basis for Specificity in Chaperone Action

(2014) G. Elif Karagöz et al.   CELL

Classification of Intrinsically Disordered Regions and Proteins

(2014) Robin van der Lee et al.   CHEMICAL REVIEWS

Many roads lead to Rome? Multiple modes of Cu,Zn superoxide dismutase destabilization, misfolding and aggregation in amyotrophic lateral sclerosis

(2014) Helen R. Broom et al.   Essays in Biochemistry

NMR contributions to structural dynamics studies of intrinsically disordered proteins

(2014) Robert Konrat   JOURNAL OF MAGNETIC RESONANCE

Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch

(2014) Alaji Bah et al.   NATURE

The amyloid state and its association with protein misfolding diseases

(2014) Tuomas P. J. Knowles et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural Basis for Protein Antiaggregation Activity of the Trigger Factor Chaperone

(2014) T. Saio et al.   SCIENCE

Conformation and dynamics of the periplasmic membrane-protein–chaperone complexes OmpX–Skp and tOmpA–Skp

(2013) Björn M Burmann et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Converging Mechanisms in ALS and FTD: Disrupted RNA and Protein Homeostasis

(2013) Shuo-Chien Ling et al.   NEURON

NMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers

(2013) A. Sekhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Probing the transient dark state of substrate binding to GroEL by relaxation-based solution NMR

(2013) D. S. Libich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformational Motions Regulate Phosphoryl Transfer in Related Protein Tyrosine Phosphatases

(2013) S. K. Whittier et al.   SCIENCE

An Interdomain Energetic Tug-of-War Creates the Allosterically Active State in Hsp70 Molecular Chaperones

(2012) Anastasia Zhuravleva et al.   CELL

Protein activity regulation by conformational entropy

(2012) Shiou-Ru Tzeng et al.   NATURE

Structure of an Intermediate State in Protein Folding and Aggregation

(2012) P. Neudecker et al.   SCIENCE

Solution structure of a minor and transiently formed state of a T4 lysozyme mutant

(2011) Guillaume Bouvignies et al.   NATURE

Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy

(2011) Jameson R Bothe et al.   NATURE METHODS

Heat shock protein 70 kDa chaperone/DnaJ cochaperone complex employs an unusual dynamic interface

(2011) A. Ahmad et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An introduction to NMR-based approaches for measuring protein dynamics

(2010) Ian R. Kleckner et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A Transient and Low-Populated Protein-Folding Intermediate at Atomic Resolution

(2010) Dmitry M. Korzhnev et al.   SCIENCE

Hidden alternative structures of proline isomerase essential for catalysis

(2009) James S. Fraser et al.   NATURE

The role of dynamic conformational ensembles in biomolecular recognition

(2009) David D Boehr et al.   Nature Chemical Biology

NMR spectroscopy brings invisible protein states into focus

(2009) Andrew J Baldwin et al.   Nature Chemical Biology

Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate

(2009) E. B. Bertelsen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Observing biological dynamics at atomic resolution using NMR

(2009) Anthony K. Mittermaier et al.   TRENDS IN BIOCHEMICAL SCIENCES

Structures of invisible, excited protein states by relaxation dispersion NMR spectroscopy

(2008) P. Vallurupalli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic equilibrium engagement of a polyvalent ligand with a single-site receptor

(2008) T. Mittag et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recognition Dynamics Up to Microseconds Revealed from an RDC-Derived Ubiquitin Ensemble in Solution

(2008) O. F. Lange et al.   SCIENCE

Fuzzy complexes: polymorphism and structural disorder in protein–protein interactions

(2007) Peter Tompa et al.   TRENDS IN BIOCHEMICAL SCIENCES