Controlling and enhancing CRISPR systems

Structure and Mechanism of a Cyclic Trinucleotide-Activated Bacterial Endonuclease Mediating Bacteriophage Immunity

(2020) Rebecca K. Lau et al.   MOLECULAR CELL

Multiple Input Sensing and Signal Integration Using a Split Cas12a System

(2020) Hannah R. Kempton et al.   MOLECULAR CELL

Clades of huge phages from across Earth’s ecosystems

(2020) Basem Al-Shayeb et al.   NATURE

An anti-CRISPR viral ring nuclease subverts type III CRISPR immunity

(2020) Januka S. Athukoralage et al.   NATURE

Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate

(2020) Stephen A. McMahon et al.   Nature Communications

Critical Anti-CRISPR Locus Repression by a Bi-functional Cas9 Inhibitor

(2020) Beatriz A. Osuna et al.   Cell Host & Microbe

CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells

(2020) Ping Lin et al.   MOLECULAR CELL

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

(2020) Nicole D. Marino et al.   NATURE METHODS

Fuse to defuse: a self-limiting ribonuclease-ring nuclease fusion for type III CRISPR defence

(2020) Aleksei Samolygo et al.   NUCLEIC ACIDS RESEARCH

Potent CRISPR-Cas9 inhibitors fromStaphylococcusgenomes

(2020) Kyle E. Watters et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity

(2020) Alexander J. Meeske et al.   SCIENCE

Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

(2020) Carmela Garcia-Doval et al.   Nature Communications

Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9

(2020) Roman S. Iwasaki et al.   Nature Communications

The dynamic interplay of host and viral enzymes in type III CRISPR-mediated cyclic nucleotide signalling

(2020) Januka S Athukoralage et al.   eLife

Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer

(2020) Caroline Mahendra et al.   Nature Microbiology

Bacterial alginate regulators and phage homologs repress CRISPR–Cas immunity

(2020) Adair L. Borges et al.   Nature Microbiology

Tetramerisation of the CRISPR ring nuclease Crn3/Csx3 facilitates cyclic oligoadenylate cleavage

(2020) Januka S Athukoralage et al.   eLife

CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification

(2019) Benjamin L. Oakes et al.   CELL

Non-specific degradation of transcripts promotes plasmid clearance during type III-A CRISPR–Cas immunity

(2019) Jakob T. Rostøl et al.   Nature Microbiology

Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits

(2019) Irmantas Mogila et al.   Cell Reports

Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry

(2019) MaryClare F. Rollins et al.   MOLECULAR CELL

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

(2019) Yalan Zhu et al.   MOLECULAR CELL

A singular system with precise dosing and spatiotemporal control of CRISPR-Cas9

(2019) Amit Choudhary et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9

(2019) Basudeb Maji et al.   CELL

Cas13-induced cellular dormancy prevents the rise of CRISPR-resistant bacteriophage

(2019) Alexander J. Meeske et al.   NATURE

Broad-spectrum enzymatic inhibition of CRISPR-Cas12a

(2019) Gavin J. Knott et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An anti-CRISPR protein disables type V Cas12a by acetylation

(2019) Liyong Dong et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cell-specific CRISPR–Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins

(2019) Mareike D Hoffmann et al.   NUCLEIC ACIDS RESEARCH

A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity

(2019) Lucy Chou-Zheng et al.   eLife

CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4 Cleavage with ApA>p Formation Terminating RNase Activity

(2019) Ning Jia et al.   MOLECULAR CELL

Structural insights into the modulatory role of the accessory protein WYL1 in the Type VI-D CRISPR-Cas system

(2019) Heng Zhang et al.   NUCLEIC ACIDS RESEARCH

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

(2019) Kale Kundert et al.   Nature Communications

Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2

(2019) Annoj Thavalingam et al.   Nature Communications

Switching the activity of Cas12a using guide RNA strand displacement circuits

(2019) Lukas Oesinghaus et al.   Nature Communications

Cell-Type-Specific CRISPR Activation with MicroRNA-Responsive AcrllA4 Switch

(2019) Moe Hirosawa et al.   ACS Synthetic Biology

Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology

(2019) Mikhail H. Hanewich-Hollatz et al.   ACS Central Science

Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription

(2019) Sabrina Y. Stanley et al.   CELL

Cyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defence

(2019) Sabine Grüschow et al.   NUCLEIC ACIDS RESEARCH

The autoregulator Aca2 mediates anti-CRISPR repression

(2019) Nils Birkholz et al.   NUCLEIC ACIDS RESEARCH

Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins

(2019) Jooyoung Lee et al.   RNA

Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a

(2019) Gavin J Knott et al.   eLife

Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas

(2019) Rafael Molina et al.   Nature Communications

Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing

(2019) Bianca Garcia et al.   Cell Reports

Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6

(2019) Olivier Fuchsbauer et al.   MOLECULAR CELL

A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases

(2019) Senén D. Mendoza et al.   NATURE

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

A jumbo phage that forms a nucleus-like structure evades CRISPR–Cas DNA targeting but is vulnerable to type III RNA-based immunity

(2019) Lucia M. Malone et al.   Nature Microbiology

Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR–Cas Systems

(2018) Mitchell R. O'Connell   JOURNAL OF MOLECULAR BIOLOGY

Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae

(2018) Erianna M. Basgall et al.   MICROBIOLOGY-SGM

Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein

(2018) Winston X. Yan et al.   MOLECULAR CELL

Systematic prediction of genes functionally linked to CRISPR-Cas systems by gene neighborhood analysis

(2018) Sergey A. Shmakov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate

(2018) Januka S. Athukoralage et al.   NATURE

Phage AcrIIA2 DNA Mimicry: Structural Basis of the CRISPR and Anti-CRISPR Arms Race

(2018) Liang Liu et al.   MOLECULAR CELL

Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function

(2018) Ka-Hei Siu et al.   Nature Chemical Biology

Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in Pseudomonas aeruginosa

(2018) Nina Molin Høyland-Kroghsbo et al.   mBio

Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28

(2017) Aaron A. Smargon et al.   MOLECULAR CELL

Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

(2017) Ole Niewoehner et al.   NATURE

Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus

(2017) Tao Liu et al.   NUCLEIC ACIDS RESEARCH

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

(2017) Migle Kazlauskiene et al.   SCIENCE

Assembly of a nucleus-like structure during viral replication in bacteria

(2017) Vorrapon Chaikeeratisak et al.   SCIENCE

Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors

(2017) Dirk A. Kleinjan et al.   Nature Communications

Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors

(2017) Toni A. Baeumler et al.   Cell Reports

Disabling Cas9 by an anti-CRISPR DNA mimic

(2017) Jiyung Shin et al.   Science Advances

Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR–Cas system

(2016) Joshua R. Elmore et al.   GENES & DEVELOPMENT

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

(2016) Adrian G. Patterson et al.   MOLECULAR CELL

Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition

(2016) Migle Kazlauskiene et al.   MOLECULAR CELL

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

Multidimensional chemical control of CRISPR–Cas9

(2016) Basudeb Maji et al.   Nature Chemical Biology

Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3

(2016) Xiaofei Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Quorum sensing controls thePseudomonas aeruginosaCRISPR-Cas adaptive immune system

(2016) Nina M. Høyland-Kroghsbo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6

(2016) Ole Niewoehner et al.   RNA

Continuous genetic recording with self-targeting CRISPR-Cas in human cells

(2016) S. D. Perli et al.   SCIENCE

Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3

(2016) Xiaofei Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity

(2015) Poulami Samai et al.   CELL

Cas9 function and host genome sampling in Type II-A CRISPR–Cas adaptation

(2015) Yunzhou Wei et al.   GENES & DEVELOPMENT

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Regulation of the Type I-F CRISPR-Cas system by CRP-cAMP and GalM controls spacer acquisition and interference

(2015) Adrian G. Patterson et al.   NUCLEIC ACIDS RESEARCH

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks

(2014) A. A. Nielsen et al.   Molecular Systems Biology

CRISPR transcriptional repression devices and layered circuits in mammalian cells

(2014) Samira Kiani et al.   NATURE METHODS

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

The CRISPR/Cas Immune System Is an Operon Regulated by LeuO, H-NS, and Leucine-Responsive Regulatory Protein in Salmonella enterica Serovar Typhi

(2011) L. Medina-Aparicio et al.   JOURNAL OF BACTERIOLOGY

Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli

(2010) Ritsdeliz Perez-Rodriguez et al.   MOLECULAR MICROBIOLOGY

H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO

(2010) Edze R. Westra et al.   MOLECULAR MICROBIOLOGY