Article
Immunology
Sophie Octavia, Ka Lip Chew, Raymond T. P. Lin, Jeanette W. P. Teo
Summary: Global travel has led to the occasional introduction of multidrug-resistant Salmonella Typhi into industrialized countries. Azithromycin-resistant Salmonella Typhi has been detected in Singapore, with 2 isolates likely being locally acquired. Ongoing vigilance and surveillance are necessary to minimize the public health risk posed by this serious pathogen.
EMERGING INFECTIOUS DISEASES
(2021)
Review
Biochemistry & Molecular Biology
Diego Sanchez-Popoca, Isela Serrano-Fujarte, Marcos Fernandez-Mora, Edmundo Calva
Summary: LeuO is a transcriptional regulator in bacteria that plays a role in stress response and virulence. This article discusses four areas of research, including the diversity of the LeuO gene, the involvement of nucleoid-associated proteins in regulation processes, the effector-binding domain of LeuO, and the role of the CRISPR-Cas system in gene regulation in S. Typhi.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Stephy Mol Robinson, Vyshakh Rajachandran, Suchismita Majumdar, Satabdi Saha, Sneha Das, Sujay Chattopadhyay
Summary: Detecting adaptive mutations leading to gene inactivation or loss of function is crucial for understanding the evolution of bacterial virulence and antibiotic resistance. Truncation mutations, which trim the length of encoded proteins, can contribute to gene inactivation. This study analyzed the accumulation of truncation mutations in two serovars of Salmonella, finding distinct sets of core truncated genes in each serovar. These truncation mutations were acquired by different subsets of isolates and were also targeted by convergent amino acid mutations in different serovars, suggesting adaptation. Further research is needed to investigate the impact of adaptive truncation mutations on bacterial virulence evolution.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Nandita Sharma, Ankita Das, Pujitha Raja, Sandhya Amol Marathe
Summary: This study investigated the regulation of biofilm by Salmonella enterica serovar Typhimurium through the CRISPR-Cas system. The results showed that the system positively regulated surface biofilm while inhibiting pellicle biofilm formation. The knockout strains exhibited altered biofilm architecture and upregulated cellulose secretion. The study also found that the CRISPR-Cas system differentially regulated the formation of surface-attached and pellicle biofilm.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Shawn T. Clark, Kirby Cronin, Antoine J. Corbeil, Samir N. Patel
Summary: This work provides an updated summary of the antimicrobial susceptibility patterns among Salmonella Typhi strains isolated from patients in Ontario, Canada. The emergence and spread of resistant strains have complicated the epidemiology and treatment of typhoid fever. A 10-year retrospective survey showed stable susceptibility patterns for certain drugs but an increase in nonsusceptibility to ciprofloxacin and ceftriaxone, indicating the need for healthcare providers to consider current trends in antimicrobial susceptibility for proper therapy selection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien
Summary: Our study aimed to investigate the effects of deleting tolC on the adhesion and invasion of HT-29 epithelial cells and THP-1 macrophages by S. Typhi in vitro. Our results showed that the tolC mutant strain had significantly reduced adhesion and invasion rates compared to the wild-type strain in both cell types. Additionally, we observed downregulation of SPI-1 gene expression in the tolC mutant.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Wan Ratmaazila Wan Wan Makhtar, Izwan Bharudin, Nurul Hidayah Samsulrizal, Nik Yusnoraini Yusof
Summary: The advancement in whole-genome sequencing technology in recent years has revolutionized the study of infectious diseases, providing new insights into various fields related to infectious diseases. By examining the genomes of Salmonella enterica serovar Typhi, researchers have enhanced their understanding of genetic evolution and pathogenic mechanisms, potentially advancing the development of typhoid vaccines and medications.
Review
Microbiology
Maryam Khan, Saba Shamim
Summary: S. Typhi is a pathogen causing typhoid fever, with over 27 million cases and 200,000 deaths globally each year. The pathogen causes infection through various virulence factors, including resistance genes, leading to resistance to multiple broad-spectrum antibiotics. The issue of antibiotic resistance has raised global concern.
Article
Chemistry, Analytical
M. S. Bacchu, M. R. Ali, S. Das, S. Akter, H. Sakamoto, S- Suye, M. M. Rahman, K. Campbell, M. Z. H. Khan
Summary: An electrochemical DNA biosensor was fabricated for the selective identification of S. Typhi in real samples. The biosensor showed a low limit of detection and the ability to be reused multiple times. The experimental results demonstrated the high feasibility and accuracy of using this biosensor for detecting S. Typhi in clinical and food samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Microbiology
Marcos Fernandez-Mora, Diego Sanchez-Popoca, Gloria Altamirano-Cruz, Grecia Lopez-Mendez, Andrea Teresa Tellez-Galicia, Carmen Guadarrama, Edmundo Calva
Summary: The study showed that the P1 promoter of the S. Typhi leuO gene is positively regulated by the (p)ppGpp alarmone, while the P3, P5, P4, P6, and P7 promoters are negatively regulated by it, providing insights into the role of multiple promoters in controlling LeuO expression.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Immunology
Hao Tang, Ziyang Zhan, Xiucheng Liu, Ying Zhang, Xinxiang Huang, Min Xu
Summary: Propionate, a major constituent of short chain fatty acids, has been found to reduce the viability of Salmonella enterica serovar Typhi (S. Typhi) in macrophages through propionylation of PhoP. This modification decreases the phosphorylation and DNA-binding ability of PhoP, resulting in reduced intracellular survival of S. Typhi. These findings provide a potential molecular mechanism by which propionate affects the virulence of S. Typhi.
MICROBIAL PATHOGENESIS
(2023)
Article
Immunology
Debayan Ganguli, Swarnali Chakraborty, Suparna Chakraborty, Ananda Pal, Animesh Gope, Santasabuj Das
Summary: Antibiotic resistance is a major threat to public health worldwide. This study investigates the temporary and reversible resistance induced by antibiotic treatment, specifically focusing on the development of L-form bacteria in response to ampicillin. The researchers cultured ampicillin-sensitive Salmonella enterica serovar Typhi Ty2 strain in increasing concentrations of ampicillin over 14 days, resulting in the development of an L-form strain with altered characteristics and increased pathogenicity. These findings highlight the potential for drug escape and cell-to-cell spread of bacteria during antibiotic treatment and the need for new approaches to reduce infection reservoirs.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
I-Chen Li, Rayean Wu, Chung-Wen Hu, Keh-Ming Wu, Zeng-Weng Chen, Chung-Hsi Chou
Summary: Studies have shown that whole genome sequencing (WGS) has the highest resolution in distinguishing Salmonella enterica serovar Schwarzengrund strains compared to traditional methods like pulsed-field gel electrophoresis (PFGE), clustered regular interspaced short palindromic repeats (CRISPR), and multilocus sequence typing (MLST).
Article
Microbiology
Jessica N. N. Uwanibe, Tolulope A. A. Kayode, Paul E. E. Oluniyi, Kazeem Akano, Idowu B. B. Olawoye, Chinedu A. A. Ugwu, Christian T. T. Happi, Onikepe A. A. Folarin
Summary: This study aims to elucidate the role of asymptomatic carriers in the transmission and persistence of typhoid fever in Nigeria and other endemic countries. The findings show a high seroprevalence of Salmonella Typhi in healthy children, but no carriage, indicating an inability to sustain transmission. It also highlights the insufficiency of using a single technique for typhoid fever surveillance in healthy children living in endemic areas.
Article
Microbiology
Liliana Medina-Aparicio, Sarahi Rodriguez-Gutierrez, Javier E. Rebollar-Flores, Angel G. Martinez-Batallar, Blanca D. Mendoza-Mejia, Eira D. Aguirre-Partida, Alejandra Vazquez, Sergio Encarnacion, Edmundo Calva, Ismael Hernandez-Lucas
Summary: This study reveals that the CRISPR-Cas system acts hierarchically on OmpR to control the synthesis of outer membrane proteins in Salmonella enterica serovar Typhi. The expression of different porins is affected differently in mutants lacking specific genes.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Immunology
Liliana Medina-Aparicio, Sonia Davila, Javier E. Rebollar-Flores, Edmundo Calva, Ismael Hernandez-Lucas
PATHOGENS AND DISEASE
(2018)
Article
Multidisciplinary Sciences
Anaid Meza-Villezcas, Ana L. Gallego-Hernandez, Fitnat H. Yildiz, Oscar E. Jaime-Acuna, Oscar Raymond-Herrera, Alejandro Huerta-Saquero
Article
Multidisciplinary Sciences
Claudia Silva, Edmundo Calva, Marcos Fernandez-Mora, Jose L. Puente, Pablo Vinuesa
Article
Multidisciplinary Sciences
R. Vazquez-Munoz, A. Meza-Villezcas, P. G. J. Fournier, E. Soria-Castro, K. Juarez-Moreno, A. L. Gallego-Hernandez, N. Bogdanchikova, R. Vazquez-Duhalt, A. Huerta-Saquero
Article
Environmental Sciences
Ana L. Gallego-Hernandez, Diana Meza-Figueroa, Judith Tanori, Monica Acosta-Elias, Belem Gonzalez-Grijalva, Juan F. Maldonado-Escalante, Sarai Rochin-Wong, Diego Soto-Puebla, Sofia Navarro-Espinoza, Roberto Ochoa-Contreras, Martin Pedroza-Montero
ENVIRONMENTAL POLLUTION
(2020)
Article
Microbiology
J. E. Rebollar-Flores, L. Medina-Aparicio, V. E. Osio-Becerro, J. M. Villarreal, S. Mayo, B. D. Mendoza, S. Rodriguez-Gutierrez, L. Olvera, S. Davila, S. Encarnacion, A. G. Martinez-Batallar, E. Calva, I Hernandez-Lucas
JOURNAL OF BACTERIOLOGY
(2020)
Article
Environmental Sciences
Diana Meza-Figueroa, Marcelino Barboza-Flores, Francisco M. Romero, Monica Acosta-Elias, Ernesto Hernandez-Mendiola, Francisco Maldonado-Escalante, Efren Perez-Segura, Belem Gonzalez-Grijalva, Mercedes Meza-Montenegro, Leticia Garcia-Rico, Sofia Navarro-Espinoza, Karla Santacruz-Gomez, Ana Gallego-Hernandez, Martin Pedroza-Montero
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Microbiology
Anahi Maldonado-Cabrera, Aracely Angulo-Molina, Ubydul Haque, Carlos Velazquez, Andrea S. Alvarez-Villasenor, Karla J. Santacruz-Gomez, Ana L. Gallego-Hernandez
Summary: A study investigated inflammation and coagulation abnormalities in young adults aged 18-40, finding that increased NMR, LMR, and C-RP levels were associated with disease aggravation. Obesity, arterial hypertension, and type-2 diabetes were related to COVID-19 severity. Additionally, some young adults presented COVID-19-associated coagulopathy.
Article
Microbiology
Isaac B. Olivar-Casique, Liliana Medina-Aparicio, Selena Mayo, Yitzel Gama-Martinez, Javier E. Rebollar-Flores, Gabriel Martinez-Batallar, Sergio Encarnacion, Edmundo Calva, Ismael Hernandez-Lucas
Summary: Salmonella enterica serovar Typhi uses multiple genetic elements to resist the presence of human bile, and enhances energy production to sustain damaged cell envelope components.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Microbiology
Marcos Fernandez-Mora, Diego Sanchez-Popoca, Gloria Altamirano-Cruz, Grecia Lopez-Mendez, Andrea Teresa Tellez-Galicia, Carmen Guadarrama, Edmundo Calva
Summary: The study showed that the P1 promoter of the S. Typhi leuO gene is positively regulated by the (p)ppGpp alarmone, while the P3, P5, P4, P6, and P7 promoters are negatively regulated by it, providing insights into the role of multiple promoters in controlling LeuO expression.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Karla Santacruz-Gomez, Rodrigo Melendrez, Marlen Licerio-Ramirez, Ana L. Gallego-Hernandez, Martin Pedroza-Montero, Ratnesh Lal
Summary: The actin cytoskeleton plays a crucial role in cancer cell metastasis. Plasmonic photothermal therapy mediated by PEGylated gold nanorods can disrupt the cytoskeleton integrity of HeLa cells and induce cell death through apoptosis.
JOURNAL OF NANOPARTICLE RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Diego Sanchez-Popoca, Isela Serrano-Fujarte, Marcos Fernandez-Mora, Edmundo Calva
Summary: LeuO is a transcriptional regulator in bacteria that plays a role in stress response and virulence. This article discusses four areas of research, including the diversity of the LeuO gene, the involvement of nucleoid-associated proteins in regulation processes, the effector-binding domain of LeuO, and the role of the CRISPR-Cas system in gene regulation in S. Typhi.
MOLECULAR MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
A. L. Gallego-Hernandez, W. H. DePas, J. H. Park, J. K. Teschler, R. Hartmann, H. Jeckel, K. Drescher, S. Beyhan, D. K. Newman, F. H. Yildiz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)