Article
Immunology
Ezhaveni Sathiyamoorthi, Jin-Hyung Lee, Yulong Tan, Jintae Lee
Summary: This study investigated the antibacterial and antivirulence effects of chromones and their derivatives against Vibrio parahaemolyticus and Vibrio harveyi. 6-Bromo-3-formylchromone (6B3FC) and 6-chloro-3-formylchromone (6C3FC) were found to be active against these bacteria and inhibited biofilm formation. 6B3FC also showed inhibition of gene expression associated with quorum sensing and biofilm formation in V. parahaemolyticus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Food Science & Technology
Wenxiu Zhu, Jingzhu Gao, Hongli Liu, Jiaxiu Liu, Tong Jin, Ningbo Qin, Xiaomeng Ren, Xiaodong Xia
Summary: Sodium butyrate exhibits antibiofilm effects against V. parahaemolyticus by inhibiting biofilm formation, disrupting cellular metabolism, reducing cell viability within the biofilm, decreasing bacterial motility and surface hydrophobicity, and interfering with quorum sensing. Additionally, sodium butyrate down-regulates genes associated with biofilm formation in V. parahaemolyticus, suggesting its potential as a potent agent in combating biofilm in food industries.
Article
Infectious Diseases
Cuifang Tian, Mengqi Yuan, Qian Tao, Tianming Xu, Jing Liu, Zhenhua Huang, Qian Wu, Yingjie Pan, Yong Zhao, Zhaohuan Zhang
Summary: Improper use of antibiotics leads to the emergence of antibiotic-resistant strains and renders the treatment of infectious diseases ineffective. This study demonstrates that Vibrio parahaemolyticus develops biofilm defense mechanisms in response to aminoglycoside antibiotics, significantly impacting their resistance to these antibiotics.
Article
Microbiology
Ezhaveni Sathiyamoorthi, Olajide Sunday Faleye, Jin-Hyung Lee, Vinit Raj, Jintae Lee
Summary: Researchers found that halogenated indoles effectively inhibited the growth and biofilm formation of Vibrio parahaemolyticus, causing visible damage to the cell membrane, suggesting potential antimicrobial and antivirulence effects against the Vibrio species.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Yawen Liu, Li Wang
Summary: This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. The results showed that PCA can inhibit biofilm formation, reduce polysaccharide production, and prevent the adhesion of V. parahaemolyticus, thereby reducing its virulence.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Xiangpeng Han, Mengyu Zhang, Jiayi Peng, Jinsong Wu, Qingping Zhong
Summary: A novel bacteriocin, plantaricin Z057, was extracted, purified, and identified from Lactiplantibacillus plantarum Z057. Plantaricin Z057 showed broad-spectrum antibacterial activity against both Gram-positive and Gram-negative pathogens, as well as heat tolerance and activity at acidic pH. It disrupted membrane integrity, caused cell deformation and cytoplasmic leakage, and significantly reduced biofilm biomass and metabolic activity of Vibrio parahaemolyticus.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Xiangpeng Han, Qingying Chen, Xingguo Zhang, Xiaolan Chen, Dongsheng Luo, Qingping Zhong
Summary: This study found that Lactiplantibacillus plantarum 2057 can effectively inhibit the formation of Vibrio parahaemolyticus biofilm and interfere with its adhesion ability. In addition, the extract of L. plantarum Z057 significantly reduces the biofilm biomass, metabolic activity, and various important components of V. parahaemolyticus. These findings suggest that L. plantarum 2057 could be a biocontrol strategy alternative to chemical methods against foodborne pathogens.
Article
Biotechnology & Applied Microbiology
Haixi Lu, Yang Sun, Xiaodi Wang, Zhong Lu, Junli Zhu
Summary: This study investigates the inhibitory effects of salinity and citral on the biofilm formation of Vibrio parahaemolyticus (VP) and its potential mechanism. The results show that combining high salinity with citral significantly reduces the biofilm formation of VP and alters the gene expression patterns related to biofilm development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Xingguo Zhang, Wendan Shangguan, Jie Wang, Zhenlin Liao, Xiang Fang, Qingping Zhong
Summary: This study discovered novel anti-biofilm agents from Lacticaseibacillus rhamnosus. The ethyl acetate extract of L. rhamnosus MS1 was found to effectively reduce the motility, extracellular polysaccharide (EPS) content, and biofilm production of Vibrio parahaemolyticus. Transcriptomic analysis revealed significant gene expression changes in V. parahaemolyticus treated with the extract, indicating the regulation of quorum sensing, biofilm formation, virulence factors, and metabolic pathways by L. rhamnosus MS1. This study demonstrates the efficacy of L. rhamnosus MS1 as an anti-biofilm agent.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Kun Chen, Chuantao Peng, Fang Chi, Chundi Yu, Qingli Yang, Zhaojie Li
Summary: Chlorogenic acid (CA), a phenolic acid found in many plants, has been shown to have antibacterial activity. This study tested the antibacterial and antibiofilm activities of CA against Yersinia enterocolitica for the first time and found that CA exhibited outstanding antibacterial activity and could inhibit biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Jingyi Dai, Ritian Jin, Jialong Gao, Jude Juventus Aweya, Rong Lin, Guiling Li, Shen Yang
Summary: The rising threat of antibiotic-resistant pathogens has led to the exploration of naturally-derived antimicrobial peptides (AMPs) as replacements for chemical preservatives in foodborne pathogen control. In this study, ten peptides were discovered from salt-fermented shrimps via ultra-performance liquid chromatography-mass spectrometry. Among them, PV-Q5 exhibited strong antibacterial activity against Vibrio parahaemolyticus and Escherichia coli, with a minimum inhibitory concentration of 31.25 μg/mL. PV-Q5 also demonstrated an ability to increase bacterial cell membrane permeability and rupture bacterial cell membranes. These findings suggest that PV-Q5 could be used in food preservation to effectively control foodborne pathogenic bacteria.
Article
Gastroenterology & Hepatology
Daniel Tchelet, Kinga Keppel, Eran Bosis, Dor Salomon
Summary: In this study, proteomics was used to identify antibacterial effectors in the secretome of Vibrio parahaemolyticus strains. The results revealed two conserved effectors and other effectors that are only present in subsets of strains, suggesting a core and accessory effector repertoire of the T6SS2 system. A conserved Rhs effector was also discovered to be required for T6SS2 activity.
Article
Environmental Sciences
Md. Ashrafudoulla, Kyung Won Na, Md. Iqbal Hossain, Md. Furkanur Rahaman Mizan, Shamsun Nahar, Sazzad Hossen Toushik, Pantu Kumar Roy, Si Hong Park, Sang -Do Ha
Summary: Infections of gastroenteritis in humans are mainly caused by consumption of shellfish contaminated with Vibrio parahaemolyticus, leading to health and economic losses. Investigation on the virulence factor production, antibiotic resistance profile, and biofilm-forming capacity of Vibrio parahaemolyticus isolates showed high resistance to antibiotics and virulence factors in isolates from shellfish in Korea. Monitoring of these factors may help in decreasing V. parahaemolyticus infections and ensuring seafood safety.
MARINE POLLUTION BULLETIN
(2021)
Article
Immunology
Xin Tan, Jun Qiao, Jianli Wang, Hedan Li, Xiaoyuan Wang
Summary: This study characterized the ampicillin-resistant genes in Vibrio parahaemolyticus ATCC33846. Transcriptomic analysis revealed significant differential expression of genes related to various metabolic pathways and bacterial secretion systems. Overexpression of specific genes enhanced V. parahaemolyticus resistance to ampicillin.
MICROBIAL PATHOGENESIS
(2022)
Article
Food Science & Technology
Meimei Fang, Ruifei Wang, Addo Keren Agyekumwaa, Yigang Yu, Xinglong Xiao
Summary: Phenyllactic acid (PLA) showed strong antibacterial effects against Vibrio parahaemolyticus by increasing bacterial membrane permeability and causing potassium leakage. It could also inhibit the motility and biofilm formation of the bacteria. Additionally, PLA was effective in eliminating V. parahaemolyticus contamination on raw salmon fillets, making it a promising candidate for controlling foodborne pathogens in chilled aquatic products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shamsun Nahar, Ha Lim Jeong, Ah Jin Cho, Jun-Ha Park, Sangha Han, Younsoo Kim, Si-Hong Park, Sang-Do Ha
Summary: This study investigated the biofilm reduction effects of a food-grade enzyme (ficin) and a common sanitizer (PAA) on Salmonella. Results showed that ficin could enhance the efficacy of PAA in reducing biofilm. The combined use of enzyme and sanitizer proved promising in addressing food safety issues.
Article
Biotechnology & Applied Microbiology
Kye-Hwan Byun, Kyung Won Na, Md Ashrafudoulla, Min Woo Choi, Sang Ha Han, Iksoon Kang, Si Hong Park, Sang-Do Ha
Summary: The combination treatment of PAA or LA with UV-C significantly reduced Salmonella Enteritidis biofilms on food contact surfaces and chicken skin.
Article
Agriculture, Dairy & Animal Science
Md. Ashrafudoulla, Mevo S. I. Ulrich, Sazzad Hossen Toushik, Shamsun Nahar, Pantu Kumar Roy, Furkanur Rahaman Mizan, Si Hong Park, Sang-Do Ha
Summary: Consumers' increasing awareness of food quality and microbial safety has driven researchers to develop non-conventional processing technologies. This review summarizes the advanced applications of high-pressure processing, ultraviolet, pulsed light, ultrasonic sterilization, pulsed electric field, irradiation, and cold plasma in producing safe and high-quality food products compared to traditional processes. These technologies offer the potential to inactivate foodborne microorganisms without compromising nutritional value, making them promising alternatives for the food industry.
WORLDS POULTRY SCIENCE JOURNAL
(2023)
Review
Food Science & Technology
Sangha Han, Seok-Woo Hyun, Jeong Won Son, Min Su Song, Dong Jae Lim, Changsun Choi, Si Hong Park, Sang-Do Ha
Summary: Various foodborne viruses have caused gastroenteritis and had a significant economic impact globally in the past decade. The emergence of new variants of infectious viruses continues to grow. Inactivating foodborne viruses in the food industry is challenging because they can survive in the food matrix during processing and storage. Conventional methods have limitations, necessitating more effective and environmentally friendly techniques. Nonthermal techniques are considered a new platform for safe and effective treatment to inactivate foodborne viruses.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Review
Food Science & Technology
Md. Anamul Hasan Chowdhury, Md. Ashrafudoulla, Senakpon Isaie Ulrich Mevo, Md. Furkanur Rahaman Mizan, Si Hong Park, Sang-Do Ha
Summary: Poultry, especially chicken meat, is thriving globally but also poses threats to human hygiene through the transmission of infectious diseases caused by major foodborne pathogens. To tackle this issue, innovative strategies such as natural compounds, bacteriophages, nanoparticles, coatings, enzymes, and probiotics are emerging to improve food safety and quality, and extend shelf-life. These strategies show potential antimicrobial activities and can enhance the immune system and poultry health. Lactoferrin, bacteriocin, antimicrobial peptides, cell-free supernatants, and biosurfactants are of considerable interest for their prospective application as natural antimicrobials in the poultry processing industry.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Food Science & Technology
Sazzad Hossen Toushik, Kyeongjun Kim, Park Sang-Hyeok, Park Jun-Ha, Md. Ashrafudoulla, Mevo Senakpon Isaie Ulrich, Md. Furkanur Rahman Mizan, Md. Iqbal Hossain, Shim Won-Bo, Iksoon Kang, Ha Sang-Do
Summary: This study investigated the antimicrobial and antibiofilm efficacy of Lactobacillus curvatus B67-produced postbiotic and quercetin against Listeria monocytogenes and Salmonella enterica ser. Typhimurium. The postbiotic showed strong antimicrobial effects mainly due to organic acids, effectively reducing pathogenic biofilm cells on processed meat surfaces. The combination of postbiotic and quercetin increased the leakage of intracellular metabolites and inhibited biofilm formation. This study suggests that postbiotic and quercetin can be used as alternative bio-protective agents in the meat processing industry.
Article
Biotechnology & Applied Microbiology
Eiseul Kim, Hae-In Jung, Si Hong Park, Hae-Yeong Kim, Soo-Ki Kim
Summary: This study reports the genome characteristics of B. contaminans SK875 for the first time, revealing the presence of virulence and quorum sensing-related genes. Comparative genomic analysis revealed 186 unique genes in SK875, including toxin higB, betA, and hypothetical proteins. These findings provide insights into the pathogenic characteristics of B. contaminans species.
Article
Biotechnology & Applied Microbiology
Jun Haeng Nam, Alyssa Thibodeau, Yanping L. L. Qian, Michael C. C. Qian, Si Hong Park
Summary: This study evaluated the potential role of three plant growth-promoting rhizobacteria (PGPR) - Bacillus subtilis, Bacillus amyloliquefaciens, and Pseudomonas monteilii - as biofertilizers. The results showed that the application of PGPR significantly increased the populations of Bacillus and Pseudomonas and promoted the growth of nitrogen-fixing bacteria. The sensory evaluation did not show significant differences, but TSS and color evaluation suggested that PGPR acted as a ripening enhancer.
Review
Multidisciplinary Sciences
Aar Rafi Mahmud, Tanzila Ismail Ema, Mohd Faijanur - Rob Siddiquee, Asif Shahriar, Hossain Ahmed, Md. Mosfeq-Ul-Hasan, Nova Rahman, Rahatul Islam, Muhammad Ramiz Uddin, Md. Furkanur Rahaman Mizan
Summary: Flavonols are phytoconstituents that have important biological and medicinal properties. They are antioxidants that may have beneficial effects in diabetes, cancer, cardiovascular disease, and viral and bacterial infections. Quercetin, myricetin, kaempferol, and fisetin are the main dietary flavonols, with quercetin having strong antioxidant abilities.
BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES
(2023)
Correction
Biotechnology & Applied Microbiology
Hyunhee Hong, Seung Min Yang, Eiseul Kim, Hyun Jung Kim, Si Hong Park
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hyunhee Hong, Seung Min Yang, Eiseul Kim, Hyun Jung Kim, Si Hong Park
Summary: This study investigates and compares the genetic characteristics between stress-resistant and -sensitive L. monocytogenes using whole genome sequencing (WGS). The functionality of unique genes in stress-resistant L. monocytogenes, such as carbohydrate and nucleotide transport and metabolism, is distinct from the stress-sensitive group. The virulence gene lisR is more prevalent in stress-resistant L. monocytogenes, and five strains possess the tet(M) antibiotic resistance gene.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Food Science & Technology
Md. Ashikur Rahman, Md. Ashrafudoulla, Shirin Akter, Si Hong Park, Sang-Do Ha
Summary: Aquaculture is an important food source with modernization leading to outbreaks of diseases in fish and economic loss. The study focuses on the association between biofilms and probiotics in aquaculture and their role in combating pathogenic bacteria. Bibliometric analysis provides valuable information for researchers and policymakers in determining research priorities.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Younsoo Kim, Shamsun Nahar, Ah Jin Cho, A. G. M. Sofi Uddin Mahamud, Si Hong Park, Sang-Do Ha
Summary: This study investigated the effect of DNase I and eugenol on the control of Salmonella Enteritidis biofilm formation on different surfaces. Results showed that the combined treatment of DNase I and eugenol was more effective in reducing biofilm formation compared to individual treatments. The study also demonstrated the potential of this approach in minimizing health risks in the duck processing plant.
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.
