MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosis

Knocking ’em Dead: Pore-Forming Proteins in Immune Defense

(2020) Xing Liu et al.   Annual Review of Immunology

Necroptosis-blocking compound NBC1 targets heat shock protein 70 to inhibit MLKL polymerization and necroptosis

(2020) Andrea N. Johnston et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of MLKL membrane translocation as a checkpoint in necroptotic cell death using Monobodies

(2020) Emma J. Petrie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distinct pseudokinase domain conformations underlie divergent activation mechanisms among vertebrate MLKL orthologues

(2020) Katherine A. Davies et al.   Nature Communications

A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction

(2020) Joanne M. Hildebrand et al.   Nature Communications

Flotillin-mediated endocytosis and ALIX–syntenin-1–mediated exocytosis protect the cell membrane from damage caused by necroptosis

(2019) Weiliang Fan et al.   Science Signaling

TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL

(2019) Ayaz Najafov et al.   MOLECULAR CELL

The AMPK–Parkin axis negatively regulates necroptosis and tumorigenesis by inhibiting the necrosome

(2019) Seung Baek Lee et al.   NATURE CELL BIOLOGY

Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins

(2019) Junghyun Lim et al.   eLife

Pannexin‐1 limits the production of proinflammatory cytokines during necroptosis

(2019) Tiphaine Douanne et al.   EMBO REPORTS

Cleavage of RIPK1 by caspase-8 is crucial for limiting apoptosis and necroptosis

(2019) Kim Newton et al.   NATURE

Viral MLKL Homologs Subvert Necroptotic Cell Death by Sequestering Cellular RIPK3

(2019) Emma J. Petrie et al.   Cell Reports

The Killer Pseudokinase Mixed Lineage Kinase Domain-Like Protein (MLKL)

(2019) James M. Murphy   Cold Spring Harbor Perspectives in Biology

K63-linked ubiquitination regulates RIPK1 kinase activity to prevent cell death during embryogenesis and inflammation

(2019) Yong Tang et al.   Nature Communications

Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease

(2019) Najoua Lalaoui et al.   NATURE

A dominant autoinflammatory disease caused by non-cleavable variants of RIPK1

(2019) Panfeng Tao et al.   NATURE

MLKL Requires the Inositol Phosphate Code to Execute Necroptosis

(2018) Cole M. Dovey et al.   MOLECULAR CELL

BiallelicRIPK1mutations in humans cause severe immunodeficiency, arthritis, and intestinal inflammation

(2018) Delphine Cuchet-Lourenço et al.   SCIENCE

Conformational switching of the pseudokinase domain promotes human MLKL tetramerization and cell death by necroptosis

(2018) Emma J. Petrie et al.   Nature Communications

Human RIPK1 deficiency causes combined immunodeficiency and inflammatory bowel diseases

(2018) Yue Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs

(2018) Shin Murai et al.   Nature Communications

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation

(2017) Seongmin Yoon et al.   IMMUNITY

TrackMate: An open and extensible platform for single-particle tracking

(2017) Jean-Yves Tinevez et al.   METHODS

MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death

(2017) Isabel Jaco et al.   MOLECULAR CELL

Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis

(2017) Jiefei Geng et al.   Nature Communications

EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation

(2017) Jaclyn S. Pearson et al.   Nature Microbiology

Short form FLICE-inhibitory protein promotes TNFα-induced necroptosis in fibroblasts derived from CFLARs transgenic mice

(2016) Ryodai Shindo et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

RIPK3 deficiency or catalytically inactive RIPK1 provides greater benefit than MLKL deficiency in mouse models of inflammation and tissue injury

(2016) K Newton et al.   CELL DEATH AND DIFFERENTIATION

Bax assembly into rings and arcs in apoptotic mitochondria is linked to membrane pores

(2016) R. Salvador-Gallego et al.   EMBO JOURNAL

Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores

(2016) Xing Liu et al.   NATURE

Tight junctions: from simple barriers to multifunctional molecular gates

(2016) Ceniz Zihni et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death

(2016) A V Jacobsen et al.   Cell Death & Disease

The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance

(2016) Jérémie Gautheron et al.   Nature Communications

Necroptosis Promotes Staphylococcus aureus Clearance by Inhibiting Excessive Inflammatory Signaling

(2016) Kipyegon Kitur et al.   Cell Reports

Necroptosis signalling is tuned by phosphorylation of MLKL residues outside the pseudokinase domain activation loop

(2015) M. C. Tanzer et al.   BIOCHEMICAL JOURNAL

Analysis of the N-terminal region of human MLKL, as well as two distinct MLKL isoforms, reveals new insights into necroptotic cell death

(2015) K. H. Arne  et al.   BIOSCIENCE REPORTS

Larazotide Acetate for Persistent Symptoms of Celiac Disease Despite a Gluten-Free Diet: A Randomized Controlled Trial

(2015) Daniel A. Leffler et al.   GASTROENTEROLOGY

BigDataViewer: visualization and processing for large image data sets

(2015) Tobias Pietzsch et al.   NATURE METHODS

A cytosolic heat shock protein 90 and cochaperone CDC37 complex is required for RIP3 activation during necroptosis

(2015) Dianrong Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The potential utility of tight junction regulation in celiac disease: focus on larazotide acetate

(2015) Shahryar Khaleghi et al.   Therapeutic Advances in Gastroenterology

RIPK1 Regulates RIPK3-MLKL-Driven Systemic Inflammation and Emergency Hematopoiesis

(2014) James A. Rickard et al.   CELL

Mixed Lineage Kinase Domain-like Protein MLKL Causes Necrotic Membrane Disruption upon Phosphorylation by RIP3

(2014) Huayi Wang et al.   MOLECULAR CELL

Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death

(2014) Joanne M. Hildebrand et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

(2014) Bi-Chang Chen et al.   SCIENCE

TNFR1-dependent cell death drives inflammation in Sharpin-deficient mice

(2014) James A Rickard et al.   eLife

Necroptosis Is Active in Children With Inflammatory Bowel Disease and Contributes to Heighten Intestinal Inflammation

(2013) Maria Pierdomenico et al.   AMERICAN JOURNAL OF GASTROENTEROLOGY

Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death

(2013) Xin Chen et al.   CELL RESEARCH

The Pseudokinase MLKL Mediates Necroptosis via a Molecular Switch Mechanism

(2013) James M. Murphy et al.   IMMUNITY

Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis

(2013) Zhenyu Cai et al.   NATURE CELL BIOLOGY

Mixed Lineage Kinase Domain-like Protein Mediates Necrosis Signaling Downstream of RIP3 Kinase

(2012) Liming Sun et al.   CELL

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

Larazotide acetate regulates epithelial tight junctions in vitro and in vivo

(2012) Shobha Gopalakrishnan et al.   PEPTIDES

A practical guide to evaluating colocalization in biological microscopy

(2011) Kenneth W. Dunn et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis

(2011) Claudia Günther et al.   NATURE

Virus Inhibition of RIP3-Dependent Necrosis

(2010) Jason W. Upton et al.   Cell Host & Microbe

The structural basis for membrane binding and pore formation by lymphocyte perforin

(2010) Ruby H. P. Law et al.   NATURE

Phosphorylation-Driven Assembly of the RIP1-RIP3 Complex Regulates Programmed Necrosis and Virus-Induced Inflammation

(2009) YoungSik Cho et al.   CELL

Receptor Interacting Protein Kinase-3 Determines Cellular Necrotic Response to TNF-α

(2009) Sudan He et al.   CELL

RIP3, an Energy Metabolism Regulator That Switches TNF-Induced Cell Death from Apoptosis to Necrosis

(2009) D.-W. Zhang et al.   SCIENCE

Reducing small intestinal permeability attenuates colitis in the IL10 gene-deficient mouse

(2008) M C Arrieta et al.   GUT

Identification of RIP1 kinase as a specific cellular target of necrostatins

(2008) Alexei Degterev et al.   Nature Chemical Biology