Genome editing approaches to augment livestock breeding programs

Spermatogonial Stem Cell Transplantation: Insights and Outlook for Domestic Animals

(2019) Mariana I. Giassetti et al.   Annual Review of Animal Biosciences

Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction

(2019) Yanxia Luo et al.   Stem Cell Research & Therapy

Diversifying the structure of zinc finger nucleases for high-precision genome editing

(2019) David E. Paschon et al.   Nature Communications

Comparison of gene editing versus conventional breeding to introgress the POLLED allele into the US dairy cattle population

(2019) M.L. Mueller et al.   JOURNAL OF DAIRY SCIENCE

Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes

(2019) Maki Hirata et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Simplified pipelines for genetic engineering of mammalian embryos by CRISPR-Cas9 electroporation†

(2019) Deqiang Miao et al.   BIOLOGY OF REPRODUCTION

Targeted gene insertion into Z chromosome of chicken primordial germ cells for avian sexing model development

(2019) Hong Jo Lee et al.   FASEB JOURNAL

Dnd1 Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production

(2019) Abdul Rasheed Baloch et al.   Animals

CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus

(2018) Huaqiang Yang et al.   ANTIVIRAL RESEARCH

An FBXO40 knockout generated by CRISPR/Cas9 causes muscle hypertrophy in pigs without detectable pathological effects

(2018) Yunlong Zou et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Establishment of protocol for preparation of gene-edited bovine ear-derived fibroblasts for somatic cell nuclear transplantation

(2018) Takeshi ISHINO et al.   BIOMEDICAL RESEARCH-TOKYO

CRISPR/Cas9-mediated specific integration of fat-1 at the goat MSTN locus

(2018) Ju Zhang et al.   FEBS Journal

Efficient genome editing in cultured cells and embryos of Debao pig and swamp buffalo using the CRISPR/Cas9 system

(2018) Xiaoping Su et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

(2018) Simon E. Tröder et al.   PLoS One

Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

(2018) Yanina Soledad Bogliotti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient generation of goats with defined point mutation (I397V) in GDF9 through CRISPR/Cas9

(2018) Yiyuan Niu et al.   REPRODUCTION FERTILITY AND DEVELOPMENT

Production of microhomologous-mediated site-specific integrated LacS gene cow using TALENs

(2018) Xiaohu Su et al.   THERIOGENOLOGY

Cattle with a precise, zygote-mediated deletion safely eliminate the major milk allergen beta-lactoglobulin

(2018) Jingwei Wei et al.   Scientific Reports

Site-Specific Fat-1 Knock-In Enables Significant Decrease of n-6PUFAs/n-3PUFAs Ratio in Pigs

(2018) Mengjing Li et al.   G3-Genes Genomes Genetics

Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

(2018) Natasa Savic et al.   eLife

Using CRISPR/Cas9 Technology Efficiently targeted of Goat Myostatin through Zygotes Microinjection result in double-muscled phenotype in goats

(2018) Zhengyi He et al.   BIOSCIENCE REPORTS

Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs

(2018) Guanghai Xiang et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Disruption of G0/G1 switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken

(2018) Tae Sub Park et al.   FASEB JOURNAL

In Vivo Genome Editing as a Therapeutic Approach

(2018) Beatrice Ho et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR/Cas9-mediated sheep MSTN gene knockout and promote sSMSCs differentiation

(2018) Yani Zhang et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

CRISPR plants now subject to tough GM laws in European Union

(2018) Ewen Callaway   NATURE

In vivo CRISPR editing with no detectable genome-wide off-target mutations

(2018) Pinar Akcakaya et al.   NATURE

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

(2018) Fuminori Tanihara et al.   PLoS One

In vitro breeding: application of embryonic stem cells to animal production†

(2018) Daniel E Goszczynski et al.   BIOLOGY OF REPRODUCTION

Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing

(2018) He Zhang et al.   DEVELOPMENT

Strict EU ruling on gene-edited crops squeezes science

(2018) Andrew J. Wight   NATURE

Harnessing genomic information for livestock improvement

(2018) Michel Georges et al.   NATURE REVIEWS GENETICS

Disruption of the ZBED6 binding site in intron 3 of IGF2 by CRISPR/Cas9 leads to enhanced muscle development in Liang Guang Small Spotted pigs

(2018) Xiaofeng Liu et al.   TRANSGENIC RESEARCH

Resistance to coronavirus infection in amino peptidase N-deficient pigs

(2018) Kristin M. Whitworth et al.   TRANSGENIC RESEARCH

A Strategy To Exploit Surrogate Sire Technology in Livestock Breeding Programs

(2018) Paolo Gottardo et al.   G3-Genes Genomes Genetics

Genetically modified pigs are protected from classical swine fever virus

(2018) Zicong Xie et al.   PLoS Pathogens

1000 Bull Genomes Project to Map Simple and Complex Genetic Traits in Cattle: Applications and Outcomes

(2018) Ben J. Hayes et al.   Annual Review of Animal Biosciences

Revolutionize livestock breeding in the future: an animal embryo-stem cell breeding system in a dish

(2018) Zhuocheng Hou et al.   Journal of Animal Science and Biotechnology

CRISPR/Cas9-mediated loss of FGF5 function increases wool staple length in sheep

(2017) Wen-Rong Li et al.   FEBS Journal

RAPID COMMUNICATION: Generation of FGF5 knockout sheep via the CRISPR/Cas9 system12

(2017) R. Hu et al.   JOURNAL OF ANIMAL SCIENCE

Deciphering, Communicating, and Engineering the CRISPR PAM

(2017) Ryan T. Leenay et al.   JOURNAL OF MOLECULAR BIOLOGY

An AANAT/ASMT transgenic animal model constructed with CRISPR/Cas9 system serving as the mammary gland bioreactor to produce melatonin-enriched milk in sheep

(2017) Teng Ma et al.   JOURNAL OF PINEAL RESEARCH

Bones Morphogenic Protein-4 and retinoic acid combined treatment comparative analysis for in vitro differentiation potential of murine mesenchymal stem cells derived from bone marrow and adipose tissue into germ cells

(2017) Maryam H. Shirzeyli et al.   MICROSCOPY RESEARCH AND TECHNIQUE

Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research

(2017) Moon-Soo Kim et al.   MOLECULES AND CELLS

The control of DNA repair by the cell cycle

(2017) Nicole Hustedt et al.   NATURE CELL BIOLOGY

Reconstitution ofUCP1using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity

(2017) Qiantao Zheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas9-mediated knockout of myostatin in Chinese indigenous Erhualian pigs

(2017) Kankan Wang et al.   TRANSGENIC RESEARCH

Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle

(2017) Mitsumi Ikeda et al.   Scientific Reports

Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene

(2017) Ki-Eun Park et al.   Scientific Reports

In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells

(2017) Ekaterina Shlush et al.   Stem Cell Research & Therapy

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

(2017) Christine Burkard et al.   PLoS Pathogens

Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule

(2017) Haroon Butt et al.   Frontiers in Plant Science

Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9

(2017) Wenjun Zhou et al.   PLoS One

Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis)

(2017) Zhongkai Cui et al.   Scientific Reports

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes

(2017) Ki-Eun Park et al.   Scientific Reports

Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

(2017) Xuemei Zhang et al.   Scientific Reports

Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System

(2017) Karim Khalil et al.   Scientific Reports

Efficient TALEN-mediated myostatin gene editing in goats

(2016) Baoli Yu et al.   BMC DEVELOPMENTAL BIOLOGY

Primordial germ cell-like cells derived from canine adipose mesenchymal stem cells

(2016) Yudong Wei et al.   CELL PROLIFERATION

Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro

(2016) Quan Zhou et al.   Cell Stem Cell

Promotion of alleles by genome editing in livestock breeding programmes

(2016) J.M. Hickey et al.   JOURNAL OF ANIMAL BREEDING AND GENETICS

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Identification of a nonsense mutation in APAF1 that is likely causal for a decrease in reproductive efficiency in Holstein dairy cattle

(2016) Heather A. Adams et al.   JOURNAL OF DAIRY SCIENCE

Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation

(2016) Zhenkui Qin et al.   MARINE BIOTECHNOLOGY

Reconstitution in vitro of the entire cycle of the mouse female germ line

(2016) Orie Hikabe et al.   NATURE

Production of hornless dairy cattle from genome-edited cell lines

(2016) Daniel F Carlson et al.   NATURE BIOTECHNOLOGY

Changes in genetic selection differentials and generation intervals in US Holstein dairy cattle as a result of genomic selection

(2016) Adriana García-Ruiz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gene targeting, genome editing: from Dolly to editors

(2016) Wenfang Tan et al.   TRANSGENIC RESEARCH

Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP

(2016) Yanzhen Bi et al.   Scientific Reports

Generation and evaluation of Myostatin knock-out rabbits and goats using CRISPR/Cas9 system

(2016) Rihong Guo et al.   Scientific Reports

Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells

(2016) Hanning Wang et al.   Scientific Reports

Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep

(2016) Xiaolong Wang et al.   Scientific Reports

Somatic cell reprogramming-free generation of genetically modified pigs

(2016) F. Tanihara et al.   Science Advances

Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells into the Testes of Infertile Male Rats and New Germ Cell Formation

(2016) Mohammad Ghasemzadeh-Hasankolaei et al.   International Journal of Stem Cells

Targeting Human α-Lactalbumin Gene Insertion into the Goat β-Lactoglobulin Locus by TALEN-Mediated Homologous Recombination

(2016) Hongmei Zhu et al.   PLoS One

Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp

(2016) Zhaomin Zhong et al.   Scientific Reports

Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon

(2016) Anna Wargelius et al.   Scientific Reports

Mammalian interspecies substitution of immune modulatory alleles by genome editing

(2016) Simon G. Lillico et al.   Scientific Reports

Targeted mutagenesis in chicken using CRISPR/Cas9 system

(2016) Isao Oishi et al.   Scientific Reports

Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer

(2016) Honghui Li et al.   Scientific Reports

Updating the reference population to achieve constant genomic prediction reliability across generations

(2015) M. Pszczola et al.   Animal

Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells

(2015) Kotaro Sasaki et al.   Cell Stem Cell

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes

(2015) Xiaoyang Zhou et al.   HUMAN MUTATION

Derivation of male germ cells from ram bone marrow mesenchymal stem cells by three different methods and evaluation of their fate after transplantation into the testis

(2015) Mohammad Ghasemzadeh-Hasankolaei et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets

(2015) Shengbin Rao et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes

(2015) M. Crispo et al.   PLoS One

Artificial cloning of domestic animals

(2015) Carol L. Keefer   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TALE nickase-mediatedSP110knockin endows cattle with increased resistance to tuberculosis

(2015) Haibo Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs

(2015) Lili Qian et al.   Scientific Reports

Production of Human Albumin in Pigs Through CRISPR/Cas9-Mediated Knockin of Human cDNA into Swine Albumin Locus in the Zygotes

(2015) Jin Peng et al.   Scientific Reports

Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System

(2015) Kankan Wang et al.   Scientific Reports

Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system

(2015) Xiaolong Wang et al.   Scientific Reports

Deletions, Inversions, Duplications: Engineering of Structural Variants using CRISPR/Cas in Mice

(2015) Katerina Kraft et al.   Cell Reports

Cell-specific ablation in the testis: what have we learned?

(2015) L. B. Smith et al.   Andrology

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Regulatory framework for gene editing and other new breeding techniques (NBTs) in Argentina

(2015) Agustina I Whelan et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

Gene targeting by TALEN-induced homologous recombination in goats directs production of β-lactoglobulin-free, high-human lactoferrin milk

(2015) Chenchen Cui et al.   Scientific Reports

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

Ectopic expression ofDAZLgene in goat bone marrow-derived mesenchymal stem cells enhances the trans-differentiation to putative germ cells compared to the exogenous treatment of retinoic acid or bone morphogenetic protein 4 signalling molecules

(2014) Guangyao Yan et al.   CELL BIOLOGY INTERNATIONAL

diRNA-Ago2-RAD51 complexes at double-strand break sites

(2014) Soichiro Yamanaka et al.   CELL RESEARCH

Potential Spermatogenesis Recovery with Bone Marrow Mesenchymal Stem Cells in an Azoospermic Rat Model

(2014) Deying Zhang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Factors Affecting the Development of Somatic Cell Nuclear Transfer Embryos in Cattle

(2014) Satoshi AKAGI et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Efficient Generation of Myostatin (MSTN) Biallelic Mutations in Cattle Using Zinc Finger Nucleases

(2014) Junjie Luo et al.   PLoS One

Efficient Gene Knockout in Goats Using CRISPR/Cas9 System

(2014) Wei Ni et al.   PLoS One

Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 20051

(2014) M. J. Zuidhof et al.   POULTRY SCIENCE

Generation of mastitis resistance in cows by targeting human lysozyme gene to  -casein locus using zinc-finger nucleases

(2014) X. Liu et al.   PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Genome edited sheep and cattle

(2014) Chris Proudfoot et al.   TRANSGENIC RESEARCH

Generation of Myostatin b Knockout Yellow Catfish (Tachysurus Fulvidraco) Using Transcription Activator-Like Effector Nucleases

(2014) Zhangji Dong et al.   Zebrafish

Performance of the Cas9 Nickase System in Drosophila melanogaster

(2014) Xingjie Ren et al.   G3-Genes Genomes Genetics

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Efficient nonmeiotic allele introgression in livestock using custom endonucleases

(2013) W. Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A new method for producing transgenic birds via direct in vivo transfection of primordial germ cells

(2013) Scott G. Tyack et al.   TRANSGENIC RESEARCH

Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned cows

(2013) Xu Liu et al.   Nature Communications

Live pigs produced from genome edited zygotes

(2013) Simon G. Lillico et al.   Scientific Reports

Accelerating Improvement of Livestock with Genomic Selection

(2013) Theo Meuwissen et al.   Annual Review of Animal Biosciences

Applied Animal Genomics: Results from the Field

(2013) Alison L. Van Eenennaam et al.   Annual Review of Animal Biosciences

Recovery of Fertility in Azoospermia Rats after Injection of Adipose-Tissue-Derived Mesenchymal Stem Cells: The Sperm Generation

(2013) Cihangir Cakici et al.   Biomed Research International

Comparison of the efficacy of three concentrations of retinoic acid for transdifferentiation induction in sheep marrow-derived mesenchymal stem cells into male germ cells

(2012) M. Ghasemzadeh-Hasankolaei et al.   ANDROLOGIA

Evaluation of Bovine Embryo Biopsy Techniques according to Their Ability to Preserve Embryo Viability

(2012) M. Cenariu et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Bovine Polledness – An Autosomal Dominant Trait with Allelic Heterogeneity

(2012) Ivica Medugorac et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The Role of Productivity in Improving the Environmental Sustainability of Ruminant Production Systems

(2012) Judith L. Capper et al.   Annual Review of Animal Biosciences

Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells

(2011) Katsuhiko Hayashi et al.   CELL

Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle

(2011) Shengli Yu et al.   CELL RESEARCH

Transgenic salmon: a final leap to the grocery shelf?

(2011) Alison L Van Eenennaam et al.   NATURE BIOTECHNOLOGY

Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases

(2011) Zhangji Dong et al.   PLoS One

Primary broiler breeding--Striking a balance between economic and well-being traits1

(2010) M. N. Katanbaf et al.   POULTRY SCIENCE

Derivation of male germ cell-like lineage from human fetal bone marrow stem cells

(2010) Jinlian Hua et al.   REPRODUCTIVE BIOMEDICINE ONLINE

Culture Conditions and Signalling Networks Promoting the Establishment of Cell Lines from Parthenogenetic and Biparental Pig Embryos

(2010) Tiziana A. L. Brevini et al.   Stem Cell Reviews and Reports

Establishment and characterization of embryonic stem-like cells from porcine somatic cell nuclear transfer blastocysts

(2010) S. Kim et al.   ZYGOTE