Article
Multidisciplinary Sciences
Thilo Kohler, Alexandre Luscher, Lena Falconnet, Gregory Resch, Robert McBride, Quynh-Anh Mai, Juliette L. Simonin, Marc Chanson, Bohumil Maco, Raphael Galiotto, Arnaud Riat, Shawna McCallin, Benjamin Chan, Christian van Delden
Summary: The study documents the successful treatment of a male patient with life-threatening multi-drug resistant Pseudomonas aeruginosa infection using personalized phage therapy and intravenous antibiotics. This indicates that phage therapy can be an alternative or complementary strategy for multidrug resistant bacterial infections.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
M. Di Vito, S. Garzoli, R. Rosato, M. Mariotti, J. Gervasoni, L. Santucci, E. Ovidi, M. Cacaci, G. Lombarini, R. Torelli, A. Urbani, M. Sanguinetti, F. Bugli
Summary: This study aims to identify an essential oil (EO) effective against C. auris. The results show that Cinnamomum zeylanicum EO has strong antifungal activity against C. auris. Further research has found that Cinnamomum zeylanicum EO can synergize with fluconazole and overcome the resistance of C. auris. In vivo experiments on Galleria mellonella larvae demonstrate the safety and efficacy-enhancing ability of Cinnamomum zeylanicum EO.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Medicinal
Ayan Mukherjee, Danyel Ramirez, Rajat Arora, Gilbert Arthur, Frank Schweizer
Summary: This study describes the synthesis and biological properties of amphiphilic tribasic galactosamines as potential rifampicin (RIF) potentiators. The results demonstrate that these compounds enhance the effectiveness of RIF against multidrug-resistant Acinetobacter baumannii and Escherichia coli in low salt-containing media, but not against Pseudomonas aeruginosa. The potentiating effect of RIF was reduced when bivalent Mg++ or Ca++ ions were added at physiological concentrations.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Orlando Poma, Betty Ricce, Jeyson Beraun, Jackson Edgardo Perez Carpio, Hugo Fernandez, Juan Soria
Summary: Plastic pollution, resulting from the long decomposition time of most single-use plastics, is a grave environmental problem. This study evaluates the efficacy of G. mellonella in biodegrading LDPE in the district of Pangoa, Junin, Peru. The results suggest that the best treatment is conditioning G. mellonella with beeswax and carrying out the treatment for 36 hours.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Enas Mamdouh Hefzy, Mahmoud A. F. Khalil, Amal A. Ibrahim Amin, Hossam M. Ashour, Yasser Fathy Abdelaliem
Summary: The study showed that BLISs from lactobacilli and streptococci have inhibitory effects on Candida species, with good stability against heat, pH, and surfactants but sensitivity to proteases. In vivo testing using Galleria mellonella larvae model demonstrated that BLISs significantly improved survival rates when treated with Candida isolates, suggesting the potential of BLISs as biotherapeutic agents in vulvovaginal candidiasis.
Article
Microbiology
Dan-Tiberiu Furnica, Silke Dittmer, Maike Isabell Sanders, Joerg Steinmann, Peter-Michael Rath, Lisa Kirchhoff
Summary: Aspergillus fumigatus has become a significant threat in clinical settings, with increasing cases of invasive infections caused by azole-resistant A. fumigatus isolates (ARAF). This study investigated the in-vitro and in-vivo activity of the imidazole luliconazole (LLCZ) against clinical ARAF. The results showed that LLCZ has inhibitory effects on the planktonic cells and early biofilms of ARAF.
Article
Immunology
Nathalia Ferreira Fregonezi, Lariane Teodoro Oliveira, Junya de Lacorte Singulani, Caroline Maria Marcos, Claudia Tavares dos Santos, Maria Lucia Taylor, Maria Jose Soares Mendes-Giannini, Haroldo Cesar de Oliveira, Ana Marisa Fusco-Almeida
Summary: This study investigated the influence of Hsp60 on biofilm features of H. capsulatum and its effect in the G. mellonella model. The results showed that the Hsp60 antibody (mAb 7B6) was effective in reducing metabolic activity and biomass of the fungus, resulting in thinner biofilms with lower cell and matrix content, as well as increased survival of infected larvae. The findings suggest a different pattern of fungus-host interaction in the invertebrate model compared to a murine model, highlighting the importance of Hsp60 in biofilm establishment and larval infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Laura Moya-Anderico, Joana Admella, Eduard Torrents
Summary: This study presents an optimized and novel protocol for clearing Galleria mellonella larvae, allowing for visualization of internalized fluorescent nanoparticles using confocal microscopy and opening doors to a wide range of applications.
Article
Biotechnology & Applied Microbiology
Adam J. Mulkern, Linda B. Oyama, Alan R. Cookson, Christopher J. Creevey, Toby J. Wilkinson, Hamza Olleik, Marc Maresca, Giarla C. da Silva, Patricia P. Fontes, Denise M. S. Bazzolli, Hilario C. Mantovani, Bamu F. Damaris, Luis A. J. Mur, Sharon A. Huws
Summary: This study evaluated the therapeutic potential of antimicrobial peptides (AMPs) from the rumen microbiome against Pseudomonas aeruginosa infections. All AMPs exhibited antimicrobial activity against the strains tested, with no decrease in activity. The AMPs Lynronne 1 and 2 showed efficacy in an in vivo infection model. These findings highlight the potential of microbiome-derived AMPs for the treatment of Pseudomonas aeruginosa infections.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Engineering, Environmental
Shuai Wang, Wei Shi, Zhichu Huang, Nihong Zhou, Yanling Xie, Yu Tang, Fuliang Hu, Guangxu Liu, Huoqing Zheng
Summary: Plastic-eating insects like the greater wax moth show promise in reducing plastic pollution through biodegradation, with the potential for enhanced metabolism with the involvement of gut microbiota. This study found that polystyrene microplastics can be depolymerized and digested in greater wax moths independently of gut microbiota, indicating efficient biodegradation by the insects' enzymes. Proposed potential metabolic pathways for polystyrene degradation in the insects' intestines provide insight for further research on enzyme identification and catalytic mechanisms.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Lynn El Haddad, Georgios Angelidakis, Justin R. Clark, Jesus F. Mendoza, Austen L. Terwilliger, Christopher P. Chaftari, Mark Duna, Serena T. Yusuf, Cynthia P. Harb, Mark Stibich, Anthony Maresso, Roy F. Chemaly
Summary: Phages specific to Vancomycin-resistant Enterococci were successfully isolated from sewage samples and found to be safe and effective in the Galleria mellonella model, making them potential agents for therapy against multidrug-resistant bacterial infections.
Article
Biochemistry & Molecular Biology
Joana Admella, Eduard Torrents
Summary: Galleria mellonella is an advantageous animal model for infection research, as it is easy and inexpensive to maintain and rear. Its use is ethically acceptable and it has a conveniently manipulable size. Additionally, its immune system shares similarities with mammalian immune systems. Hemocytes, the immune cells, play a crucial role in encapsulating and eliminating pathogens and foreign particles. However, culturing G. mellonella hemocytes in vitro presents challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Javier A. Garcia, Kiem Vu, George R. Thompson, Angie Gelli
Summary: This study examined the growth and morphology of a Coccidioides posadasii strain and revealed unexpected cellular changes. The findings suggest that this strain is a useful model for studying the mechanisms underlying the transition from parasitic to saprophytic growth.
Article
Immunology
Vanessa Rafaela Milhomem Cruz-Leite, Mariana Vieira Tomazett, Juliana Santana de Curcio, Nicolau Sbaraini, Alexandre Melo Bailao, Relber Aguiar Goncales, Dayane Moraes, Maristela Pereira, Marilene Henning Vainstein, Augusto Schrank, Roberta Peres da Silva, Matthias Brock, Celia Maria de Almeida Soares
Summary: The study aimed to generate bioluminescent reporter strains of Paracoccidioides spp. by incorporating a thermostable luciferase gene under the control of different promoters. The transformation experiments and detection of luciferase confirmed the successful construction of the strains. Additionally, an in vivo model was proposed for the systemic study of paracoccidioidomycoses.
MICROBES AND INFECTION
(2022)
Article
Immunology
Guillaume Menard, Astrid Rouillon, Gevorg Ghukasyan, Mathieu Emily, Brice Felden, Pierre-Yves Donnio
Summary: Utilizing a G. mellonella infection model allows for rapid and effective research on the roles of Staphylococcus aureus sRNAs during infection, providing important insights into the involvement of sRNAs as transcriptional regulators in staphylococcal virulence.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Temilolu Idowu, Pranati Samadder, Gilbert Arthur, Frank Schweizer
CHEMISTRY AND PHYSICS OF LIPIDS
(2016)
Article
Biochemistry & Molecular Biology
Makanjuola Ogunsina, Pranati Samadder, Temilolu Idowu, Gilbert Arthur, Frank Schweizer
Article
Chemistry, Medicinal
Temilolu Idowu, Pranati Samadder, Gilbert Arthur, Frank Schweizer
JOURNAL OF MEDICINAL CHEMISTRY
(2017)
Article
Chemistry, Medicinal
Makanjuola Ogunsina, Pranati Samadder, Temilolu Idowu, Gilbert Arthur, Frank Schweizer
JOURNAL OF MEDICINAL CHEMISTRY
(2017)
Article
Chemistry, Medicinal
Yinfeng Lyu, Xuan Yang, Sudeep Goswami, Bala Kishan Gorityala, Temilolu Idowu, Ronald Domalaon, George G. Zhanel, Anshan Shan, Frank Schweizer
JOURNAL OF MEDICINAL CHEMISTRY
(2017)
Review
Microbiology
Ronald Domalaon, Temilolu Idowu, George G. Zhanel, Frank Schweizer
CLINICAL MICROBIOLOGY REVIEWS
(2018)
Review
Pharmacology & Pharmacy
George G. Zhanel, Alyssa R. Golden, Sheryl Zelenitsky, Karyn Wiebe, Courtney K. Lawrence, Heather J. Adam, Temilolu Idowu, Ronald Domalaon, Frank Schweizer, Michael A. Zhanel, Philippe R. S. Lagace-Wiens, Andrew J. Walkty, Ayman Noreddin, Joseph P. Lynch, James A. Karlowsky
Article
Infectious Diseases
Temilolu Idowu, Derek Ammeter, Gilbert Arthur, George G. Zhanel, Frank Schweizer
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Chemistry, Medicinal
Xuan Yang, Derek Ammeter, Temilolu Idowu, Ronald Domalaon, Marc Brizuela, Oreofe Okunnu, Liting Bi, Yanelis Acebo Guerrero, George G. Zhanel, Ayush Kumar, Frank Schweizer
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Derek Ammeter, Temilolu Idowu, George G. Zhanel, Frank Schweizer
JOURNAL OF ANTIBIOTICS
(2019)
Article
Chemistry, Medicinal
Temilolu Idowu, Derek Ammeter, Heather Rossong, George G. Zhanel, Frank Schweizer
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Microbiology
Temilolu Idowu, George G. Zhanel, Frank Schweizer
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Biochemistry & Molecular Biology
Makanjuola Ogunsina, Pranati Samadder, Temilolu Idowu, Mark Nachtigal, Frank Schweizer, Gilbert Arthur
Review
Infectious Diseases
Temilolu Idowu, Frank Schweizer
Article
Chemistry, Medicinal
Shuang Mei, Su Jiang, Yuting Wang, Han Jing, Peng Yang, Miao-Miao Niu, Jindong Li, Kai Yuan, Yan Zhang
Summary: This study identifies a dual-targeting peptide, AP-1, that effectively inhibits variants of concern (VOCs) of SARS-CoV-2 without impairing host cell viability. The findings suggest that AP-1 could be a promising broad-spectrum agent for treating emerging VOCs of SARS-CoV-2.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Hyeonjun Lee, Ju Yeon Lee, Hyunsoo Jang, Hye Young Cho, Minhee Kang, Sang Hyun Bae, Suin Kim, Eunji Kim, Jaebong Jang, Jin Young Kim, Young Ho Jeon
Summary: By using liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance experiments, we identified new chemical moieties that bind to the target sites of the protein of interest, allowing for reversible binding and protein degradation. This method has the potential to expand the application of PROTAC technology.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Yingying Li, Xiyou Du, Xinru Kong, Yuelin Fang, Zhijing He, Dongzhu Liu, Hang Wu, Jianbo Ji, Xiaoye Yang, Lei Ye, Guangxi Zhai
Summary: This study proposes a novel nanoplatform based on the autophagy cascade to overcome the obstacles in chemo-immunotherapy. The platform combines chemotherapy and starvation therapy to initiate pro-death autophagy and enhance antigen presentation, while also remodeling the immunosuppressive tumor microenvironment. Furthermore, the study discovers a new therapeutic direction for the respiration inhibitor 3-bromopyruvic acid (3BP) in cancer treatment. Overall, this study offers an opportunity to improve antitumor efficacy and boost immune responses.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Bingsi Wang, Mingxu Ma, Yusen Dai, Pengfei Yu, Liang Ye, Wenyan Wang, Chunjie Sha, Huijie Yang, Yingjie Yang, Yunjing Zhu, Lin Dong, Shujuan Wei, Linlin Wang, Jingwei Tian, Hongbo Wang
Summary: Breast cancer is a common malignant tumor in women, and drug resistance remains a clinical challenge. In this study, a novel compound, G-5b, was developed with potent antagonistic and degradation activities comparable to the current drug fulvestrant. G-5b also showed improved stability and solubility. Mechanistically, G-5b engages the proteasome pathway to degrade ER, inhibiting the ER signaling pathway and inducing apoptosis and cell cycle arrest. In animal models, G-5b exhibited superior pharmacokinetics and pharmacodynamics properties. Overall, G-5b is a promising long-acting SERD worthy of further investigation and optimization.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Karoline B. Waitman, Larissa C. de Almeida, Marina C. Primi, Jorge A. E. G. Carlos, Claudia Ruiz, Thales Kronenberger, Stefan Laufer, Marcia Ines Goettert, Antti Poso, Sandra V. Vassiliades, Vinicius A. M. de Souza, Monica F. Z. J. Toledo, Neuza M. A. Hassimotto, Michael D. Cameron, Thomas D. Bannister, Leticia Costa-Lotufo, Joa o A. Machado-Neto, Mauricio T. Tavares, Roberto Parise-Filho
Summary: A series of hybrid inhibitors combining pharmacophores of known kinase inhibitors and benzohydroxamate HDAC inhibitors were synthesized and evaluated for their anticancer activity and pharmacokinetic properties. Compounds 4d-f exhibited promising cytotoxicity against hematological cells and moderate activity against solid tumor models. Compound 4d showed potent inhibition of multiple kinase targets and had stable interactions with HDAC and members of the JAK family. These compounds showed selective cytotoxicity with minimal effects on non-tumorigenic cells and favorable pharmacokinetic profiles.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Michal Sulik, Diana Fontinha, Dietmar Steverding, Szymon Sobczak, Michal Antoszczak, Miguel Prudencio, Adam Huczynski
Summary: This study describes the synthesis of the first-in-class ivermectin derivatives obtained through derivatization of the C13 position, along with the unexpected rearrangement of the macrolide ring. These derivatives show potential for antiparasitic activity and are important for the development of new antiparasitic agents.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Jun Liu, Qiu-Xian Chen, Wen-Fu Wu, Dong Wang, Si -Yu Zhao, Jia-Hao Li, Yi-Qun Chang, Shao-Gao Zeng, Jia-Yi Hu, Yu-Jie Li, Jia-Xin Du, Shu-Meng Jiao, Hai-Chuan Xiao, Qiang Zhang, Jun Xu, Jian-Fu Zhao, Hai -Bo Zhou, Yong-Heng Wang, Jian Zou, Ping-Hua Sun
Summary: A new anti-infective drug strategy has been discovered to attenuate virulence and modulate inflammation caused by drug-resistant Pseudomonas aeruginosa infections. Compound 5f inhibits biofilm formation, macrophage migration, and inflammatory response induced by P. aeruginosa, showing potential as a novel candidate against drug-resistant infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Liuzeng Chen, Ke Wang, Lingyun Wang, Wei Wang, Lifan Wang, Jia Li, Xiaohan Liu, Mengya Wang, Banfeng Ruan
Summary: In this study, a series of novel anti-inflammatory compounds were designed and synthesized based on the natural product pterostilbene skeleton. Among them, compound 8 showed the highest activity and exhibited its effects through inhibition of pro-inflammatory cytokines by blocking the NF-KB/MAPK signaling pathway. Compound 8 also demonstrated a good relieving effect on acute colitis in mice and showed good safety in acute toxicity experiments.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Si-Min Liang, Gui-Bin Liang, Hui-Ling Wang, Hong Jiang, Xian-Li Ma, Jian-Hua Wei, Ri-Zhen Huang, Ye Zhang
Summary: A series of novel multi-target antitumor agents were designed, synthesized, and evaluated. Some compounds exhibited significant antitumor activity and one compound showed excellent efficacy, limited toxicity, and low resistance. Further mechanism studies revealed that the compound exerted antitumor effects through multiple pathways.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)