A clearing protocol for Galleria mellonella larvae: Visualization of internalized fluorescent nanoparticles

Light scattering is a challenge for imaging three-dimensional organisms. A number of new tissue clearing methodologies have been described in recent years, increasing the utilities of clearing techniques to obtain transparent samples. Here, we describe the optimization of a suitable and novel protocol for clearing Galleria mellonella larvae, an alternative infection animal model with a promising potential for the toxicological evaluation of different molecules and materials. This has allowed the visualization of internalised fluorescent nano particles using confocal microscopy, opening the door to a wide range of different applications.

Automated summary

This study presents an optimized and novel protocol for clearing Galleria mellonella larvae, allowing for visualization of internalized fluorescent nanoparticles using confocal microscopy and opening doors to a wide range of applications.