Article
Biochemistry & Molecular Biology
Nan Wang, Yingshuang Liu, Chaohua Dong, Yugang Zhang, Suhua Bai
Summary: Plant MAPK cascade plays a critical role in regulating plant immunity and disease resistance. This study investigates the function of MAPK cascade in apple resistance to Botryosphaeria dothidea and identifies MdMAPKKK1 as an important regulator. The activation of the MAPK cascade and enhanced expression of MdMAPKKK1 are observed upon B. dothidea infection. Further experiments reveal that MdMAPKKK1 overexpression leads to pathogen-independent cell death, while MdMAPKKK1 silencing decreases apple resistance to B. dothidea. The interaction between MdMAPKKK1 and MdBSK1, as well as their phosphorylation, is found to be critical for resistance to B. dothidea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiang-li Dong, Zi-zhen Cheng, Wei-feng Leng, Bao-hua Li, Xiang-ming Xu, Sen Lian, Cai-xia Wang
Summary: Recent research has found that Botryosphaeria dothidea can cause both cankers and warts on apple trees. Warts are caused by fungal infection inducing hyperplasia and suberization of bark tissues, while cankers result from rapid growth of hyphae.
Article
Biotechnology & Applied Microbiology
Bei Wu, Fei Shen, Xuan Wang, Wen Yan Zheng, Chen Xiao, Yang Deng, Ting Wang, Zhen Yu Huang, Qian Zhou, Yi Wang, Ting Wu, Xue Feng Xu, Zhen Hai Han, Xin Zhong Zhang
Summary: This study aimed to identify gene markers based on QTLs and develop GAP models for predicting flesh firmness and crispness retainability in apples. The study found that genetic variations in genes like MdERF3 and MdERF118 can affect fruit texture attributes.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Han Jiang, Qi-Jun Ma, Ming-Shuang Zhong, Huai-Na Gao, Yuan-Yuan Li, Yu-Jin Hao
Summary: In this study, MdPAT16 was identified as a key player in mediating salt stress response in apple plants through palmitoylation of target proteins. Overexpression of MdPAT16 improved plant salt stress resistance and promoted the accumulation of soluble sugars. Furthermore, MdPAT16 was shown to interact with MdCBL1 and stabilize its protein levels through palmitoylation, suggesting a potential missing link in the plant salt stress response pathway.
Article
Food Science & Technology
Bruna Klein, Quellen Machado Ribeiro, Fabio Rodrigo Thewes, Rogerio de Oliveira Anese, Fernanda de Candido de Oliveira, Ingrid Duarte dos Santos, Stephanie Reis Ribeiro, Jossie Zamperetti Donadel, Auri Brackmann, Juliano Smanioto Barin, Alexandre Jose Cichoski, Roger Wagner
Summary: The study examined the effects of 1-MCP on the wax composition and metabolism of apples, showing that 1-MCP reduced ethylene production and concentrations of fatty acids and terpenoids, with no significant impact on respiratory quotient. Moreover, 1-MCP application led to greater mass loss in apples stored in dynamic controlled atmosphere.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Agronomy
Connor Lane, Yosef Al Shoffe, Jenny Kao-Kniffin, Christopher B. Watkins
Summary: The study investigated the effects of a plant growth regulator and low O2 controlled atmosphere storage on the surface microbiome of 'Gala' apples. It was found that the storage conditions influenced the composition, diversity, and function of the microbiomes inhabiting fruit surfaces. The results provide a greater understanding of biocontrol and pathogen population dynamics.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Plant Sciences
Xingqiang Fan, Yuqi Li, Cecilia H. H. Deng, Shiyao Wang, Zijun Wang, Yi Wang, Changpeng Qiu, Xuefeng Xu, Zhenhai Han, Wei Li
Summary: Propagation through stem cuttings is a popular method for clonal propagation of certain tree species. This study investigates the regulatory mechanisms of adventitious root formation in apple trees. The research identifies the gene MdBRC1 as a key player in inhibiting adventitious root formation. The study also proposes potential target genes and the use of CRISPR technology to enhance adventitious root formation in woody plants.
Article
Food Science & Technology
Caiping Huang, Bangzhu Peng
Summary: A high-performance nitrogen-doped chitosan-TiO2 nanocomposite was successfully prepared by a simple precipitation method and showed significant photocatalytic activities for the degradation of patulin in apple juice. The nanocomposite effectively degraded patulin within 35 minutes under ultraviolet irradiation, with little impact on the quality of apple juice.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Alexandra Buergy, Agnes Rolland-Sabate, Alexandre Leca, Catherine M. G. C. Renard
Summary: The impact of apple cultivars and processing methods on the texture of purees was investigated in this study. Viscosity of purees varied significantly between different cultivars, with Granny Smith cultivar showing the highest viscosity due to its firm fruits and large particles. Mealy Braeburn apples had lower viscosity purees compared to non-mealy ones, attributed to reduced cell adhesion. Processing method also affected the viscosity of purees, with high temperature-low shear process resulting in more viscous purees.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Plant Sciences
Hui Wang, Shuang Wang, Miao-Miao Fan, Shu-Hui Zhang, Lu-Long Sun, Zheng-Yang Zhao
Summary: Bagging is an important technique for producing high-quality fruits, but it can lead to browning in apple peel due to changes in microenvironment, destruction of cell structure, decrease in flavonoids, and increase in triterpenoids.
Article
Biochemistry & Molecular Biology
Yifeng Feng, Shanshan Li, Rongjian Jia, Jie Yang, Qiufang Su, Zhengyang Zhao
Summary: Exposure to high-intensity sunlight can lead to sunburn on fruits, causing damage to peel cell structure and color changes. Fruits initially try to enhance photosynthetic protection ability and antioxidant enzyme activity in response to external injury.
Article
Agronomy
Burak E. Algul, Yosef Al Shoffe, DoSu Park, William B. Miller, Christopher B. Watkins
Summary: Preharvest application of 1-MCP reduced internal ethylene concentrations and starch pattern indices in apples, leading to a faster decrease in stress watercore incidence and severity in treated fruit compared to untreated fruit.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Agronomy
Md Shipon Miah, Chloe Hinson, Macarena Farcuh
Summary: This research evaluated the horticultural performance of 'Buckeye Gala' apple scion grafted onto different rootstocks. The results showed that different rootstocks have significant impacts on tree size, yield, and fruit maturity. Considering the importance of rootstocks in fruit maturity and quality, it is crucial to take them into account when making management decisions in Western Maryland orchards.
Article
Horticulture
Yosef Al Shoffe, DoSu Park, Burak E. Algul, Christopher B. Watkins
Summary: In order to reduce the development of low temperature disorders such as soft scald, 'Honeycrisp' apples are conditioned at 10°C for 7 days before storage at 3°C. However, conditioning can increase the risk of bitter pit development. Previous research showed promise in storing fruit at 0.5°C before transferring to 3°C. This study investigates this strategy over three years.
SCIENTIA HORTICULTURAE
(2023)
Article
Nutrition & Dietetics
Matthew P. G. Barnett, Wayne Young, Kelly Armstrong, Diane Brewster, Janine M. Cooney, Stephanie Ellett, Richard Espley, William Laing, Paul Maclean, Tony McGhie, Greg Pringle, Nicole C. Roy, Lynnette R. Ferguson
Summary: The study suggests that consuming anthocyanin-rich apples may influence immune function compared to control apples, with potential changes associated with differences in the faecal microbiota.
Article
Green & Sustainable Science & Technology
Yushan Li, Ting Wu, Hui Shen, Shiwen Yang, Yi Qin, Zongmin Zhu, Long Zheng, Xianjie Wen, Minggui Xia, Xianze Yin
Summary: A Janus photothermal porous fibrous membrane is developed for efficient solar-driven seawater desalination, showing high salt rejection rate, stable solar-thermal conversion efficiency, and antibacterial activity, with potential applications in environmental protection.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Shuai Wang, Ting Wang, Qiqi Li, Chen Xu, Ji Tian, Yi Wang, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Ting Wu
Summary: This study reveals the molecular mechanisms of Chl degradation and fruit coloration in apple fruits. The transcription factor MdERF17 interacts with and is phosphorylated by MdMPK4, and the phosphorylation of MdERF17 is necessary for its transcriptional regulatory activity and regulation of Chl degradation. MdMPK4 is activated by darkness and is involved in the dark-induced degreening of fruit peels. Variation in Ser repeat insertion in MdERF17 regulates fruit peel degreening through control of the degree of MdERF17 phosphorylation by MdMPK4 during light/dark transitions.
Article
Plant Sciences
Jiaxuan Yu, Kainan Qiu, Wenjing Sun, Tuo Yang, Ting Wu, Tingting Song, Jie Zhang, Yuncong Yao, Ji Tian
Summary: In this study, a long noncoding RNA (lncRNA), MdLNC610, was found to act as a positive regulator in the regulation of ethylene biosynthesis and anthocyanin accumulation in apple fruit under high-light treatment.
Article
Plant Sciences
Jing Yang, Qingbo Zheng, Yi Wang, Ting Wu, Wei Li, Changpeng Qiu, Xuefeng Xu, Xi Zhang, Zhenhai Han, Xinzhong Zhang
Summary: Glutathione dependent protein post-translational modifications play important roles in plant development and stress response. The protein modifications differed considerably between the juvenile and the adult phase in apple, mainly localized in chloroplast and mitochondrion.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biotechnology & Applied Microbiology
Yifan Xing, Wenjing Sun, Yuying Sun, Jialin Li, Jie Zhang, Ting Wu, Tingting Song, Yuncong Yao, Ji Tian
Summary: This study investigated the dynamics of apple fruit phosphoproteome in response to light using high-performance liquid chromatography-tandem mass spectrometry analysis. The findings revealed that mitogen-activated protein kinase MdMPK6 directly interacted with and activated transcription factor MdHY5 through phosphorylation under light conditions, thereby promoting anthocyanin accumulation in apple fruit.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Qiran Sun, Longmei Zhai, Danrui Zhao, Min Gao, Yue Wu, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: The kinase MxMPK4-1 and IQ-motif containing protein MxIQM3 form a complex that enhances PM H+-ATPase activity to improve plant's response to iron deficiency stress.
Article
Plant Sciences
Xiaona Wang, Xiaofen Chai, Beibei Gao, Cecilia Deng, Catrin S. Gunther, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: Nitrogen is essential for plant growth, but increased nitrogen fertilizer application can decrease nitrogen use efficiency and waste resources. In this study, we investigated the mechanism of nitrogen uptake in apple rootstocks under low-nitrogen stress using multi-omics approaches. We found that the transcription factor bHLH130 regulates the flavonoid pathway, thereby influencing nitrogen uptake efficiency.
Article
Plant Sciences
Qinyuan Zhu, Jiahong Lv, Yue Wu, Mengqun Peng, Xinyi Wu, Jie Li, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Yi Wang, Yi Feng
Summary: The MdbZIP74 gene in apple plants negatively regulates osmotic tolerance and its silencing enhances adaptability to moderate drought conditions. It maintains redox balance by regulating cytokinin levels.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Fei Shen, Hao He, Xin Huang, Yang Deng, Xiaozeng Yang
Summary: In this study, we assembled and annotated the chicory genome using multiple sequencing technologies, and scanned its (epi)genomic features. Our results showed that bursts of highly active retrotransposon families played a crucial role in shaping the (epi)genomic characteristics. The highly methylated genome with its unique 3D structure potentially influenced critical biological processes. Comparative genomics analysis revealed the genetic basis for the rich sesquiterpene content in chicory and indicated convergent evolution in fructan biosynthesis in angiosperms.
Article
Biochemistry & Molecular Biology
Cong Liu, Jian-Zhong Lin, Yan Wang, Ye Tian, He-Ping Zheng, Zheng-Kun Zhou, Yan-Biao Zhou, Xiao-Dan Tang, Xin-Hui Zhao, Ting Wu, Shi-Long Xu, Dong-Ying Tang, Ze-Cheng Zuo, Hang He, Lian-Yang Bai, Yuan-Zhu Yang, Xuan-Ming Liu
Summary: A protein phosphatase, PC1, dephosphorylates Catalase C to negatively regulate H2O2 homeostasis in rice. PC1 inhibits the tetramerization and activity of Catalase C, leading to hypersensitivity to salt and oxidative stresses. Knockout of PC1 improves salt tolerance and rice grain yield under salt stress conditions.
Article
Plant Sciences
Qiran Sun, Danrui Zhao, Min Gao, Yue Wu, Longmei Zhai, Shan Sun, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: Iron (Fe) deficiency has a significant impact on the growth, development, fruit yield, and quality of apples. In this study, the researchers found that the plasma membrane H+-ATPase MxHA2 plays a role in promoting H+ secretion and root acidification in apple rootstocks under Fe deficiency stress. The kinase MxMPK6-2 is also involved in Fe absorption and interacts with MxHA2. The researchers discovered that MxMPK6-2 positively regulates PM H+-ATPase activity in apple roots, enhancing root acidification under Fe deficiency stress. Phosphorylation of MxHA2 by MxMPK6-2 and the phosphorylation of the Fe deficiency-induced transcription factor MxbHLH104 play a role in enhancing MxHA2 upregulation and PM H+-ATPase activity.
Article
Biochemistry & Molecular Biology
Jiahong Lv, Yi Feng, Lizhong Jiang, Guibin Zhang, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Yi Wang, Zhenhai Han
Summary: In this study, a genome-wide comprehensive analysis of WOX genes in nine Rosaceae species was conducted, and their potential roles in Malus were investigated. The results revealed significant collinearity in Prunus. domestica among the Rosaceae species. The study also categorized the cis-elements in the WOX gene family into three categories and found that overexpression of MdWOX13-1 increased callus weight and enhanced ROS scavenging against drought stress by directly binding to the MdMnSOD promoter.
Article
Plant Sciences
Keting Li, Longmei Zhai, Lizhong Jiang, Qiran Sun, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: This study found that Fe deficiency induces the expression of MxROP1 in apple at both transcriptional and translational levels. Overexpression of MxROP1, MxROP1DN, and MxROP1CA in apple roots increased ferric chelate reductase activity and the ability to acidify the rhizosphere. MxROP1 negatively regulated the stability of MxZR3.1 protein and affected its interaction with MxbHLH39.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Keting Li, Longmei Zhai, Sitong Fu, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: In this study, six MdZR genes were identified in apple. The MdZRs were classified into three categories, and MdZR2.2 was found to be significantly upregulated under salt and drought stress. Overexpression of MdZR2.2 improved apple's tolerance to drought and salt stress and their ability to scavenge reactive oxygen species (ROS). Silencing MdZR2.2 in transgenic apple roots resulted in reduced growth and decreased ability to scavenge ROS under salt and drought stress.
Article
Plant Sciences
Yimei Huang, Xiaofen Chai, Xiaona Wang, Beibei Gao, Hui Li, Zhenhai Han, Xuefeng Xu, Xinzhong Zhang, Ting Wu, Yi Wang
Summary: Microorganisms play a crucial role in the growth, health, and ecological function of apples. This study used the Illumina Miseq platform to analyze the bacterial communities in different ecological niches of two scion/rootstock combinations of apples. The results showed significant effects of different niches and rootstock genotypes on the diversity and composition of the bacterial community. Soil properties and plant characters were found to influence the structure of the bacterial community. This study provides valuable insights into the microbial-plant interaction of apple rootstocks.
HORTICULTURAL PLANT JOURNAL
(2023)