Current state-of-the-art in plant-based antibody production systems

Enhancing the Secretion of a Glyco-Engineered Anti-CD20 scFv-Fc Antibody in Hairy Root Cultures

(2018) Chiara Lonoce et al.   Biotechnology Journal

CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose

(2018) Julia Jansing et al.   PLANT BIOTECHNOLOGY JOURNAL

Enhancing the Secretion of a Glyco-Engineered Anti-CD20 scFv-Fc Antibody in Hairy Root Cultures

(2018) Chiara Lonoce et al.   Biotechnology Journal

Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins

(2017) Uri Hanania et al.   PLANT BIOTECHNOLOGY JOURNAL

N-Glycosylation of an IgG antibody secreted by Nicotiana tabacum BY-2 cells can be modulated through co-expression of human β-1,4-galactosyltransferase

(2017) Catherine Navarre et al.   TRANSGENIC RESEARCH

Plant-made virus-like particle vaccines bearing the hemagglutinin of either seasonal (H1) or avian (H5) influenza have distinct patterns of interaction with human immune cells in vitro

(2017) Hilary E. Hendin et al.   VACCINE

Alga-Made Anti-Hepatitis B Antibody Binds to Human Fcγ Receptors

(2017) Gaëtan Vanier et al.   Biotechnology Journal

Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans

(2017) Sébastien Mercx et al.   Frontiers in Plant Science

Heterologous expression of the N-acetylglucosaminyltransferase I dictates a reinvestigation of the N-glycosylation pathway in Chlamydomonas reinhardtii

(2017) Gaëtan Vanier et al.   Scientific Reports

Transient Production of Recombinant Pharmaceutical Proteins in Plants: Evolution and Perspectives

(2017) Lilya Kopertekh et al.   CURRENT MEDICINAL CHEMISTRY

Antibody Production in Plants and Green Algae

(2016) Vidadi Yusibov et al.   Annual Review of Plant Biology

Outlook in the application of Chlamydomonas reinhardtii chloroplast as a platform for recombinant protein production

(2016) Shabnam Shamriz et al.   Biotechnology & Genetic Engineering Reviews

Production of a tumour-targeting antibody with a human-compatible glycosylation profile inN. benthamianahairy root cultures

(2016) Chiara Lonoce et al.   Biotechnology Journal

Recombinant biologic products versus nutraceuticals from plants - a regulatory choice?

(2016) Pascal M. W. Drake et al.   BRITISH JOURNAL OF CLINICAL PHARMACOLOGY

A plant-derived quadrivalent virus like particle influenza vaccine induces cross-reactive antibody and T cell response in healthy adults

(2016) Stéphane Pillet et al.   CLINICAL IMMUNOLOGY

A Randomized, Controlled Trial of ZMapp for Ebola Virus Infection

(2016)   NEW ENGLAND JOURNAL OF MEDICINE

Plant-produced biopharmaceuticals: A case of technical developments driving clinical deployment

(2016) G. P. Lomonossoff et al.   SCIENCE

Production of a tumour-targeting antibody with a human-compatible glycosylation profile inN. benthamianahairy root cultures

(2016) Chiara Lonoce et al.   Biotechnology Journal

Transgene Expression in Microalgae—From Tools to Applications

(2016) Lior Doron et al.   Frontiers in Plant Science

Putting the Spotlight Back on Plant Suspension Cultures

(2016) Rita B. Santos et al.   Frontiers in Plant Science

Overcoming low yields of plant-made antibodies by a protein engineering approach

(2015) Julia Zischewski et al.   Biotechnology Journal

Molecular pharming’s foot in the FDA’s door: Protalix’s trailblazing story

(2015) Tsafrir S. Mor   BIOTECHNOLOGY LETTERS

High-value products from plants: the challenges of process optimization

(2015) Rainer Fischer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Plants as Factories for Human Pharmaceuticals: Applications and Challenges

(2015) Jian Yao et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Mannose receptor-mediated delivery of moss-made α-galactosidase A efficiently corrects enzyme deficiency in Fabry mice

(2015) Jin-Song Shen et al.   JOURNAL OF INHERITED METABOLIC DISEASE

Characterization of a chemically modified plant cell culture expressed human α-Galactosidase-A enzyme for treatment of Fabry disease

(2015) Tali Kizhner et al.   MOLECULAR GENETICS AND METABOLISM

Large-scale production of pharmaceutical proteins in plant cell culture-the protalix experience

(2015) Yoram Tekoah et al.   PLANT BIOTECHNOLOGY JOURNAL

Moss-made pharmaceuticals: from bench to bedside

(2015) Ralf Reski et al.   PLANT BIOTECHNOLOGY JOURNAL

Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants

(2015) Julian K-C. Ma et al.   PLANT BIOTECHNOLOGY JOURNAL

Commercial-scale biotherapeutics manufacturing facility for plant-made pharmaceuticals

(2015) Barry R. Holtz et al.   PLANT BIOTECHNOLOGY JOURNAL

Hairy root biotechnology—indicative timeline to understand missing links and future outlook

(2015) Shakti Mehrotra et al.   PROTOPLASMA

Clinical Safety and Immunogenicity of Tumor-Targeted, Plant-Made Id-KLH Conjugate Vaccines for Follicular Lymphoma

(2015) Daniel Tusé et al.   Biomed Research International

Overcoming low yields of plant-made antibodies by a protein engineering approach

(2015) Julia Zischewski et al.   Biotechnology Journal

Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor

(2014) Nicole Raven et al.   BIOTECHNOLOGY AND BIOENGINEERING

The therapeutic monoclonal antibody market

(2014) Dawn M Ecker et al.   mAbs

Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp

(2014) Xiangguo Qiu et al.   NATURE

Glyco-engineering for biopharmaceutical production in moss bioreactors

(2014) Eva L. Decker et al.   Frontiers in Plant Science

Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals

(2014) Elodie Mathieu-Rivet et al.   Frontiers in Plant Science

Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody

(2013) Suvi T. Häkkinen et al.   BIOTECHNOLOGY AND BIOENGINEERING

Molecular Farming of Pharmaceutical Proteins Using Plant Suspension Cell and Tissue Cultures

(2013) Stefan Schillberg et al.   CURRENT PHARMACEUTICAL DESIGN

N-Glycosylation of Plant-produced Recombinant Proteins

(2013) Dirk Bosch et al.   CURRENT PHARMACEUTICAL DESIGN

Accumulation of secreted antibodies in plant cell cultures varies according to the isotype, host species and culture conditions

(2013) Bertrand Magy et al.   PLANT BIOTECHNOLOGY JOURNAL

Optimization of BY-2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response surface method

(2013) Nikolay Vasilev et al.   PLANT BIOTECHNOLOGY JOURNAL

Correlation of ADCC activity with cytokine release induced by the stably expressed, glyco-engineered humanized Lewis Y-specific monoclonal antibody MB314

(2012) Ralf Kircheis et al.   mAbs

An engineered diatom acting like a plasma cell secreting human IgG antibodies with high efficiency

(2012) Franziska Hempel et al.   Microbial Cell Factories

Green factory: Plants as bioproduction platforms for recombinant proteins

(2011) Jianfeng Xu et al.   BIOTECHNOLOGY ADVANCES

Strategies for high-level recombinant protein expression in transgenic microalgae: A review

(2010) Gabriel Potvin et al.   BIOTECHNOLOGY ADVANCES

Transient expression systems for plant-derived biopharmaceuticals

(2010) Tatiana V Komarova et al.   Expert Review of Vaccines

Industrialization of mAb production technology: The bioprocessing industry at a crossroads

(2010) Brian Kelley   mAbs

Seed-based expression systems for plant molecular farming

(2010) Joseph Boothe et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of antibodies in plants: status after twenty years

(2010) Benoit De Muynck et al.   PLANT BIOTECHNOLOGY JOURNAL

Rapid Transient Production in Plants by Replicating and Non-Replicating Vectors Yields High Quality Functional Anti-HIV Antibody

(2010) Frank Sainsbury et al.   PLoS One

Viral vectors for production of recombinant proteins in plants

(2008) Chiara Lico et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure

(2008) Richard Strasser et al.   PLANT BIOTECHNOLOGY JOURNAL

Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans

(2007) Thomas Rademacher et al.   PLANT BIOTECHNOLOGY JOURNAL