Extending the application of biocatalysis to meet the challenges of drug development

Mapping the substrate scope of monoamine oxidase (MAO-N) as a synthetic tool for the enantioselective synthesis of chiral amines

(2018) Susanne Herter et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Biocatalysts for the pharmaceutical industry created by structure-guided directed evolution of stereoselective enzymes

(2018) Guangyue Li et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Hydrogen-Borrowing Alcohol Bioamination with Coimmobilized Dehydrogenases

(2018) Wesley Böhmer et al.   ChemCatChem

Whole-Cell Cascade Biotransformations for One-Pot Multistep Organic Synthesis

(2018) Shuke Wu et al.   ChemCatChem

Editorial overview: Biocatalysis and biotransformation: The golden age of biocatalysis

(2018) Nicholas J Turner et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

(De)hydratases — recent developments and future perspectives

(2018) Rebecca M Demming et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Enzymatic amidation for industrial applications

(2018) Brent M Dorr et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Halogenases: powerful tools for biocatalysis (mechanisms applications and scope)

(2018) Danai S Gkotsi et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Carboxylic acid reductase enzymes (CARs)

(2018) Margit Winkler   CURRENT OPINION IN CHEMICAL BIOLOGY

Lead Diversification at the Nanomole Scale Using Liver Microsomes and Quantitative Nuclear Magnetic Resonance Spectroscopy: Application to Phosphodiesterase 2 Inhibitors

(2018) R. Scott Obach et al.   JOURNAL OF MEDICINAL CHEMISTRY

Enantioselective Desymmetrization of Methylenedianilines via Enzyme-Catalyzed Remote Halogenation

(2018) James T. Payne et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Organic synthesis provides opportunities to transform drug discovery

(2018) David C. Blakemore et al.   Nature Chemistry

Getting Momentum: From Biocatalysis to Advanced Synthetic Biology

(2018) Christoffel P.S. Badenhorst et al.   TRENDS IN BIOCHEMICAL SCIENCES

Flow Bioreactors as Complementary Tools for Biocatalytic Process Intensification

(2018) Lucia Tamborini et al.   TRENDS IN BIOTECHNOLOGY

Late-Stage Microsomal Oxidation Reduces Drug–Drug Interaction and Identifies Phosphodiesterase 2A Inhibitor PF-06815189

(2018) Antonia F. Stepan et al.   ACS Medicinal Chemistry Letters

A Rational Active-Site Redesign Converts a Decarboxylase into a C═C Hydratase: “Tethered Acetate” Supports Enantioselective Hydration of 4-Hydroxystyrenes

(2018) Stefan E. Payer et al.   ACS Catalysis

P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping

(2018) Carlos G. Acevedo-Rocha et al.   ACS Catalysis

Planning chemical syntheses with deep neural networks and symbolic AI

(2018) Marwin H. S. Segler et al.   NATURE

Vicinal Diamines as Smart Cosubstrates in the Transaminase-Catalyzed Asymmetric Amination of Ketones

(2017) Stefan E. Payer et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction

(2017) Matthew Wilding et al.   GREEN CHEMISTRY

Real-Time Screening of Biocatalysts in Live Bacterial Colonies

(2017) Cunyu Yan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enantioselective Hydrogen Atom Transfer: Discovery of Catalytic Promiscuity in Flavin-Dependent ‘Ene’-Reductases

(2017) Braddock A. Sandoval et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

DNA sequencing at 40: past, present and future

(2017) Jay Shendure et al.   NATURE

Structural and functional insights into asymmetric enzymatic dehydration of alkenols

(2017) Bettina M Nestl et al.   Nature Chemical Biology

Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis

(2017) Deepankar Gahloth et al.   Nature Chemical Biology

A fully automated flow-based approach for accelerated peptide synthesis

(2017) Alexander J Mijalis et al.   Nature Chemical Biology

Enantioselective, intermolecular benzylic C–H amination catalysed by an engineered iron-haem enzyme

(2017) Christopher K. Prier et al.   Nature Chemistry

A reductive aminase from Aspergillus oryzae

(2017) Godwin A. Aleku et al.   Nature Chemistry

Directing evolution: the next revolution in drug discovery?

(2017) Andrew M. Davis et al.   NATURE REVIEWS DRUG DISCOVERY

Simple and Versatile Laboratory Scale CSTR for Multiphasic Continuous-Flow Chemistry and Long Residence Times

(2017) Michael R. Chapman et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Automated glycan assembly using the Glyconeer 2.1 synthesizer

(2017) Heung Sik Hahm et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Anti-Markovnikov alkene oxidation by metal-oxo–mediated enzyme catalysis

(2017) Stephan C. Hammer et al.   SCIENCE

Design of a synthetic yeast genome

(2017) Sarah M. Richardson et al.   SCIENCE

Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

(2017) Randall A. Hughes et al.   Cold Spring Harbor Perspectives in Biology

Biocatalysis in the Pharmaceutical Industry: The Need for Speed

(2017) Matthew D. Truppo   ACS Medicinal Chemistry Letters

On the design of complex drug candidate syntheses in the pharmaceutical industry

(2017) Martin D. Eastgate et al.   Nature Reviews Chemistry

Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes

(2017) Nicholas J. Weise et al.   Scientific Reports

Gram-Scale Synthesis of Chiral Cyclopropane-Containing Drugs and Drug Precursors with Engineered Myoglobin Catalysts Featuring Complementary Stereoselectivity

(2016) Priyanka Bajaj et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Myoglobin-Catalyzed Olefination of Aldehydes

(2016) Vikas Tyagi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Asymmetric Ketone Reduction by Imine Reductases

(2016) Maike Lenz et al.   CHEMBIOCHEM

A New Generation of Smart Amine Donors for Transaminase-Mediated Biotransformations

(2016) Andrew Gomm et al.   CHEMISTRY-A EUROPEAN JOURNAL

Squalene-hopene cyclases—evolution, dynamics and catalytic scope

(2016) Per-Olof Syrén et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The evolution of drug design at Merck Research Laboratories

(2016) Frank K. Brown et al.   JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN

Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases

(2016) Titu Devamani et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Abiological catalysis by artificial haem proteins containing noble metals in place of iron

(2016) Hanna M. Key et al.   NATURE

Accessing non-natural reactivity by irradiating nicotinamide-dependent enzymes with light

(2016) Megan A. Emmanuel et al.   NATURE

Directed evolution of artificial metalloenzymes for in vivo metathesis

(2016) Markus Jeschek et al.   NATURE

The coming of age of de novo protein design

(2016) Po-Ssu Huang et al.   NATURE

Identification of (S)-selective transaminases for the asymmetric synthesis of bulky chiral amines

(2016) Ioannis V. Pavlidis et al.   Nature Chemistry

Case Studies Illustrating a Science and Risk-Based Approach to Ensuring Drug Quality When Using Enzymes in the Manufacture of Active Pharmaceuticals Ingredients for Oral Dosage Form

(2016) Andrew S. Wells et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

An artificial metalloenzyme with the kinetics of native enzymes

(2016) P. Dydio et al.   SCIENCE

Directed evolution of cytochrome c for carbon–silicon bond formation: Bringing silicon to life

(2016) S. B. Jennifer Kan et al.   SCIENCE

Integrated catalysis opens new arylation pathways via regiodivergent enzymatic C–H activation

(2016) Jonathan Latham et al.   Nature Communications

Synthesis of Imidazolidin-4-ones via a Cytochrome P450-Catalyzed Intramolecular C–H Amination

(2016) Xinkun Ren et al.   ACS Catalysis

One-Pot Cascade Synthesis of Mono- and Disubstituted Piperidines and Pyrrolidines using Carboxylic Acid Reductase (CAR), ω-Transaminase (ω-TA), and Imine Reductase (IRED) Biocatalysts

(2016) Scott P. France et al.   ACS Catalysis

Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways

(2016) Scott P. France et al.   ACS Catalysis

When directed evolution met ancestral enzyme resurrection

(2016) Miguel Alcalde   Microbial Biotechnology

Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution

(2015) Hans Renata et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution of Enzymes for Industrial Biocatalysis

(2015) Joanne L. Porter et al.   CHEMBIOCHEM

Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions

(2015) Rohana Abu et al.   ChemCatChem

Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries

(2015) Erica Elisa Ferrandi et al.   FEBS Journal

Scaling-Up of “Smart Cosubstrate” 1,4-Butanediol Promoted Asymmetric Reduction of Ethyl-4,4,4-trifluoroacetoacetate in Organic Media

(2015) Ralf Zuhse et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades

(2015) F. G. Mutti et al.   SCIENCE

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

(2015) Pierre-Yves Colin et al.   Nature Communications

Chiral Amine Synthesis Using ω-Transaminases: An Amine Donor that Displaces Equilibria and Enables High-Throughput Screening

(2014) Anthony P. Green et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables the Enantioselective Formal Synthesis of Levomilnacipran

(2014) Z. Jane Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enzymatic Halogenation of Tryptophan on a Gram Scale

(2014) Marcel Frese et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Localized Tolerance in the Substrate Specificity of the Fluorinase Enzyme enables “Last-Step”18F Fluorination of a RGD Peptide under Ambient Aqueous Conditions

(2014) Stephen Thompson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Artificial metalloenzymes for enantioselective catalysis

(2014) Jeffrey Bos et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A microbial biomanufacturing platform for natural and semisynthetic opioids

(2014) Kate Thodey et al.   Nature Chemical Biology

Correction: Chylomicrons Promote Intestinal Absorption and Systemic Dissemination of Dietary Antigen (Ovalbumin) in Mice

(2014) Yuehui Wang et al.   PLoS One

Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations

(2014) Georg Steinkellner et al.   Nature Communications

Discovery of Potent Parthenolide-Based Antileukemic Agents Enabled by Late-Stage P450-Mediated C—H Functionalization

(2013) Joshua N. Kolev et al.   ACS Chemical Biology

Enantioselective Intramolecular CH Amination Catalyzed by Engineered Cytochrome P450 Enzymes In Vitro and In Vivo

(2013) John A. McIntosh et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biocatalytic retrosynthesis

(2013) Nicholas J Turner et al.   Nature Chemical Biology

A supramolecular approach to combining enzymatic and transition metal catalysis

(2013) Z. Jane Wang et al.   Nature Chemistry

Preparative access to medicinal chemistry related chiral alcohols using carbonyl reductase technology

(2013) Andrew S. Rowan et al.   TETRAHEDRON-ASYMMETRY

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Synthetic cascades are enabled by combining biocatalysts with artificial metalloenzymes

(2012) V. Köhler et al.   Nature Chemistry

Use of Enzymes in the Manufacture of Active Pharmaceutical Ingredients—A Science and Safety-Based Approach To Ensure Patient Safety and Drug Quality

(2012) Andrew S. Wells et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures

(2012) Eva Chovancova et al.   PLoS Computational Biology

Coupled chemo(enzymatic) reactions in continuous flow

(2011) Ruslan Yuryev et al.   Beilstein Journal of Organic Chemistry

Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution

(2011) Julie B. Rannes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Asymmetric synthesis of chiral cyclic amine from cyclic imine by bacterial whole-cell catalyst of enantioselective imine reductase

(2010) Koichi Mitsukura et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

MISER chromatography (multiple injections in a single experimental run): the chromatogram is the graph

(2010) Christopher J. Welch et al.   TETRAHEDRON-ASYMMETRY

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Chemo-enzymatic fluorination of unactivated organic compounds

(2008) Andrea Rentmeister et al.   Nature Chemical Biology